Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,234	0	G	T	49.4%	96.4 / 49.7	81	L25F (TTG→TTT)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (10/31);  new base T (40/0);  total (50/31)
Fisher's exact test for biased strand distribution p-value = 6.02e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245086‑1245384                                                                                                                                                      |                                                                                                                                                        tCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTg                                                                                                                                                         <  2:1178011/149‑1 (MQ=255)      atCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCt                                                                                                                                                      >  2:497323/1‑147 (MQ=255)        ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCt                                                                                                                                                 <  1:1143881/150‑1 (MQ=255)          gATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTAt                                                                                                                                              >  2:239754/1‑151 (MQ=255)            tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTAtt                                                                                                                                             >  1:351262/1‑150 (MQ=255)                gTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACa                                                                                                                                        <  1:157195/151‑1 (MQ=255)                   ggTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTTTTTAACAttt                                                                                                                                     >  2:1106075/1‑151 (MQ=255)                     ttCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCCTTTTTTACAtttt                                                                                                                                    >  2:730500/1‑149 (MQ=255)                          ggTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTg                                                                                                                              <  1:1369428/151‑1 (MQ=255)                           gTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGGTTTTTGGAAATTTATAAtt                                                                                                                               >  1:394652/1‑149 (MQ=255)                           gTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGa                                                                                                                             <  1:708328/151‑1 (MQ=255)                            tAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑GTTTGGTGGGGCTTATTTAACAATTAAAATTGAt                                                                                                                            >  1:286285/1‑151 (MQ=255)                                  caacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATcc                                                                                                                      <  2:486254/151‑1 (MQ=255)                                      aGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTATACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTTAAAATTTAGAATTTATCAATCCTTCt                                                                                                                  >  1:535712/1‑151 (MQ=255)                                          tCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAACATTTTAAATTGATCAATccct                                                                                                                    >  2:1383418/1‑143 (MQ=255)                                          tCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTTAAAATTTAGAATTGATTAAACCTTCt                                                                                                                  >  2:885447/1‑147 (MQ=255)                                            cTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTGGGCTTATTTAACATTTTGAAATGATTAATCCtt                                                                                                                    >  1:465518/1‑143 (MQ=255)                                             ttttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTGAACATTTAGAATTTATCAATCCTTCttg                                                                                                                >  2:404175/1‑144 (MQ=255)                                              tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCGAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGa                                                                                                            <  1:978295/149‑1 (MQ=255)                                               ttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCGTTTTGAAAATTTTGAATTGATCAAAACTTCTTGCAgggt                                                                                                          >  2:1078786/1‑147 (MQ=255)                                               ttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGAtt                                                                                                          <  1:1465395/150‑1 (MQ=255)                                                tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTa                                                                                                         <  1:229518/150‑1 (MQ=255)                                                 ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTaa                                                                                                        <  1:375921/150‑1 (MQ=255)                                                  tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAg                                                                                                       <  1:1189379/150‑1 (MQ=255)                                                    attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCCTATTGAAAATTTAGAATTGATCAATTCTTTTTGCAGATTAAGcc                                                                                                     >  1:171807/1‑150 (MQ=255)                                                      tattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaa                                                                                                   <  2:1121755/150‑1 (MQ=255)                                                       attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaaa                                                                                                  <  2:258884/150‑1 (MQ=255)                                                       attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaaa                                                                                                  <  1:1227381/150‑1 (MQ=255)                                                         tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaaaa                                                                                                 >  1:386367/1‑149 (MQ=255)                                                             tCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTTTGGCAGATTAAGCCAAAATAacc                                                                                            >  1:409496/1‑150 (MQ=255)                                                              cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGt                                                                                          <  1:1261599/151‑1 (MQ=255)                                                               gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGtt                                                                                         <  1:57679/151‑1 (MQ=255)                                                                 tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGGGCCTATTTTACATTTAGAATTGATTAATCCTTCTTGCCGGTTAAGGCAAAAAACCCGtt                                                                                         >  2:530528/1‑149 (MQ=255)                                                                  gttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTGCGCTTTTTTAACATTTAGAATTGTTCAAACCTTCTGGCAGAATAAACCAAAATACCCGTTCg                                                                                       >  1:973237/1‑150 (MQ=255)                                                                     tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTATTTAAAATTTAGAATTGATTAATCCTTCTGGCAGATTAAGACAAAAAAACCGTTCGGCa                                                                                    >  2:1312080/1‑150 (MQ=255)                                                                     tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTGCGCTTTTTTAACATTTAGAATTGGTCAAACCTTCTGGCAGATTAAGACAAAATAACCGTTCGGc                                                                                     >  1:1247491/1‑149 (MQ=255)                                                                           gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTc                                                                             <  1:409058/151‑1 (MQ=255)                                                                                tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGtt                                                                         <  1:165767/150‑1 (MQ=255)                                                                                 cAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTa                                                                        <  2:1451374/150‑1 (MQ=255)                                                                                   gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                                                     >  2:698842/1‑151 (MQ=255)                                                                                    aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAACATTTTTAAATTATCAATCCTTCTTGCAGATTTAAGCAAAAAACCCGTTTGGCAAATCTTCAGTTAtgcg                                                                    >  1:1039223/1‑151 (MQ=255)                                                                                    aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTGCGCCTATTGAAAATTTAGAATTTATCAAAACCTCTTGCAGATTAAGCCAAAAAACCCCTTCGGCAATGCTTTAGtt                                                                         >  2:218046/1‑146 (MQ=255)                                                                                       ataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTGAAAATTTAGAATTTATCAATCCTTCTTGCAGATTAAGCCAAAATAACCGTTCGGCAATGCTTCAGtt                                                                         >  2:292352/1‑143 (MQ=255)                                                                                        taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGCGCGCTTATTGAACATTTAGAATTTATCAATCCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCAATTCTTCAGTTAAGCGaa                                                                  >  2:470742/1‑149 (MQ=255)                                                                                          aataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTATTGAACATTTAGAATTTATCAATCCTTCTGGGAGATTAAGCCAAAATAACCGTTCGGGAATGCTTCAGTTACGCGGAGAgg                                                              >  1:96382/1‑151 (MQ=255)                                                                                             atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTTCGCTTTTTTAACATTTTTAATTGATCAATCCTTCTTGCCGATTAAGCCCAAATACCCGTTCGGCAATTCTTCAGTTTCGCCGagag                                                               >  1:143389/1‑147 (MQ=255)                                                                                              ttttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCCGCGCTTATTGAACAATTAGAATTTGTCAATCCTTCTGGGCGATTAAACCAAAATAACCCTTCGGCAATGCCTCAGtttccccgagagg                                                              >  1:664594/1‑136 (MQ=255)                                                                                               tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCCTTTTTAAAATTTTGAATTGATCAATCCTTCTTGCAGATTAAACCCAAAAACCCCTTCGGCAATGCTTTAGTTAAGCGGAGAggga                                                            >  2:355254/1‑146 (MQ=255)                                                                                               tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATg                                                          <  2:351216/150‑1 (MQ=255)                                                                                               tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCCTATTTAAAATTTAGAATTGATCAATCCTTCTGGGAGATTAAGGCAAAATAACCGTTCGGGAATGCTTTAGTTACGCGGAGAGGGATgg                                                         >  2:154342/1‑151 (MQ=255)                                                                                                  aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTTAAAATTTAGAATTGATCAATCCCTCTTGCAGATTAAGCCAAAAAACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGGATGGGCt                                                      >  2:33487/1‑151 (MQ=255)                                                                                                  aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCt                                                      <  2:273962/151‑1 (MQ=255)                                                                                                    aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTGAAAATTTATAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCAATGCTTCAGTTACGCGGAGAGGGATgg                                                         >  1:532419/1‑146 (MQ=255)                                                                                                    aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCCATGCTTCAGTTAAGCGGAGAGGGATGGTCTg                                                     >  2:1429313/1‑150 (MQ=255)                                                                                                     cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTTCGCCTATTGAACATTTAGAATTTATTAAAACTTTTGGGAGATTAAGCCAAAAAACCCGTTCGGCCAAGCTTCTGTTACGCGAAGAGGAATGGTCt                                                      >  2:777920/1‑148 (MQ=255)                                                                                                      aGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCGGCGCTTTTTTAACATTTAAAATTGATCAATCCTTCTTGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTCCGTTACGCGGAGAGGAATGGGCTGaa                                                   >  2:69877/1‑150 (MQ=255)                                                                                                       gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCGGCGGCTATTGGAAAATTTGAATTGATCAATACTTTTGGCAGATTAAGCCAAAAAAACCGTTCGGCAATGGTTCAGTTACGCGAAGAgggaagggatgaa                                                   >  2:639941/1‑138 (MQ=255)                                                                                                       gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtt                                                 <  1:530690/151‑1 (MQ=255)                                                                                                        tAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑ATTTTGCCGGGCTTATTGAACATTTTAAAGTTGTCAAATCTTCTTGCCGATTTAGCCAAAAAACCCGTTCGGCGATGCTTCAGTTTCGCGAAGAGGGATgg                                                         >  2:455694/1‑142 (MQ=255)                                                                                                         aGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTTTTGAAAATTTAAAATTGATTAAACCCTTTTGCAGATTAAGCCAAAAAACCCCTTCGGCAATGCTTCAGTTACGCGGAGGGGGAAGGGCTGAAtt                                                 >  2:34269/1‑149 (MQ=255)                                                                                                         aGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAACATTTAGAATTTATCAATCCTTCTGGCAGATTAAACCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAAAGGGCTGAATTC‑t                                              >  1:399964/1‑151 (MQ=255)                                                                                                         aGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTATTTAACATTTAGAATTGATCAAACCTTCTTGCAGATTAAACCAAAATACCCCTTCGGCCATTCTTCAATTACGCGAAGAGGAATGGGCTGAAtt                                                 >  1:1003235/1‑149 (MQ=255)                                                                                                          gCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑tt                                             <  2:383176/151‑1 (MQ=255)                                                                                                            cAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTGCGCTTTTTGAACATTTAGAATTGATCAAACCTTCTGGCAGATTAAACCAAAATAACCGTTCGGCAATGCTTCAGTTACGCGAAGAGGGAAGGGCTGAATtt‑tttt                                           >  1:151878/1‑151 (MQ=255)                                                                                                            cAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑tttt                                           <  1:1044277/151‑1 (MQ=255)                                                                                                             aGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑ttttt                                          <  2:344444/151‑1 (MQ=255)                                                                                                                cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGGGCGCTTATTGAACATTTAGAAATGATCAAACCTTCTGGCAGAGTAAGCCAAAAAAAACCGTCGGGAAAAGTTCAGTTAAGGGCAGAGGAATGGAGTGAAttt‑ttttttt                                        >  1:1156365/1‑150 (MQ=255)                                                                                                                cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑ttttttt                                        <  1:1292323/150‑1 (MQ=255)                                                                                                                    cGTTAAAAATGGGGCGGATAGTGAGGCGGATTTTGGC‑GCGCCTTTTGCACAATTTTAATAGAATCATTCTACTTGCAGATTAAGCAAAAAAACACGTTTCGCCATGCTTCTGTTACCCGGAGAGGAGTGGTGTGAATTC‑TTTTTTTTGt                                     >  2:1117549/1‑149 (MQ=255)                                                                                                                    cGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAAAATTTAGAAATGATCAATTCTTCTGGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTCAGTTACGCGGAGAGGAATGGGCTGAATTC‑ttttttttggt                                    >  2:74529/1‑146 (MQ=255)                                                                                                                      ttAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGc                                  <  1:885535/150‑1 (MQ=255)                                                                                                                        aaaaaTGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTaa                               <  1:530588/151‑1 (MQ=255)                                                                                                                             tGGGGCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTTAACATTTAGAATTGATTAATCCTTCTGGCAGATTAAGCCCAAAAAACCCTTCGGGCAAGCTTCAGTTTAGCGGAGAGGGATGGTCTGAATTT‑TTTTTTTTGTTGCTTAAtttt                          >  2:1397024/1‑149 (MQ=255)                                                                                                                              ggggCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTATTTAACATTTAGAAATGATCAATCCTTCTGGCAGATTAAGCCAAAAAAACCCTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTT‑TTTTTTTTGTTGGTAAAtt                            >  1:838632/1‑148 (MQ=255)                                                                                                                              ggggCGGATAGTGAGGCGG‑TTTTTGCTGCGCTTTTTGTACATTTTGAATTGATTAATCCTTCTGGCAGATTAAGCCAAAAAAACCGTTTGGCAAAGCTTCAGTTACGCGGAGAGGAATGGTCTGAATTC‑TTTTTTTTGTTTCTaaaattt                          >  2:107435/1‑146 (MQ=255)                                                                                                                                 gCGGATAGTGAGGCGG‑TTTTTGGTGCGCTTATTTAACATTTAGAATTTATCAAACCTTCTGGCAGATTTAACCCAAAAACCCGTTCGGCAATGCTTCAGTTACGCGGAGAGGGAAGGTCTGAATTC‑TTTTTTTTGTTGGTAAATTTTCTgg                      >  2:48938/1‑151 (MQ=255)                                                                                                                                    gATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAACTCTCTGGAgt                   <  1:239777/151‑1 (MQ=255)                                                                                                                                      tAGTGAGGCGG‑TTTTTGCTTCGCTTATTGAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAAACCCGTTCGGCAATGCTTCAGTTAGGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTTTCTgggg                    >  2:497607/1‑146 (MQ=255)                                                                                                                                        gTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCt               <  1:355302/151‑1 (MQ=255)                                                                                                                                                   tttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgt      >  2:431984/1‑151 (MQ=255)                                                                                                                                                    ttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg     <  1:497381/150‑1 (MQ=255)                                                                                                                                                     tGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGa    <  1:1383522/150‑1 (MQ=255)                                                                                                                                                      ggCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  <  1:730579/151‑1 (MQ=255)                                                                                                                                                      ggCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat   <  1:395871/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        TCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTC‑TTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245086‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑