Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	16,804	T→C	I34V (ATC→GTC)	hokC ←	toxic membrane protein, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	16,804	0	T	C	100.0%	312.1 / NA	94	I34V (ATC→GTC)	hokC	toxic membrane protein, small
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/47);  total (47/47)

CGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATG  >  NC_000913/16656‑16950                                                                                                                                                      |                                                                                                                                                    cgAGGCATCGTTTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAcgt                                                                                                                                                   >  1:1010776/1‑151 (MQ=255) cgAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAcgt                                                                                                                                                   >  2:378221/1‑151 (MQ=255) cgAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAcgt                                                                                                                                                   >  1:1296236/1‑151 (MQ=255)  gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAcgtg                                                                                                                                                  >  1:133041/1‑151 (MQ=255)   tgccATCTTTTTAACGAGCCCCCGCGGCGTCGCATTCTCAGGATGTTTCACCCCTTAATTTACCCCTTTTGGTAT‑CCCTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAcgtg                                                                                                                                                  <  2:421838/147‑1 (MQ=255)    ggCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGa                                                                                                                                                 >  1:1032026/1‑150 (MQ=255)      cATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAAcc                                                                                                                                              >  2:110210/1‑151 (MQ=255)       aTCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCt                                                                                                                                             >  1:1303487/1‑151 (MQ=255)            cTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTcac                                                                                                                                          >  2:1467332/1‑149 (MQ=255)              tAACGAGGCACCGAGGCGTCGCTTTCTTCAGATGGTTCACCCCTTAAGTTAGCGCTTATGGGCT‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACag                                                                                                                                        <  1:314206/149‑1 (MQ=37)                  gAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCt                                                                                                                                  >  2:1179439/1‑151 (MQ=255)                      cACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTtc                                                                                                                              >  2:667495/1‑151 (MQ=255)                        cggAGGCGTCGCATTCTTCTGATGGTTCACCCCTAAATTTAGCGCTTAGGGAAT‑CACCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCtttt                                                                                                                               <  2:1104529/146‑1 (MQ=255)                         cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCg                                                                                                                           >  2:1380888/1‑151 (MQ=255)                          gAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGt                                                                                                                          >  2:674796/1‑151 (MQ=255)                          gAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGt                                                                                                                          >  1:1373778/1‑151 (MQ=255)                            gggggcGCATTCTTCAGATGTTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CTCTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGtt                                                                                                                         <  2:443620/145‑1 (MQ=255)                            ggCGTCGCATTCTTCGGATGGTTAACCCCTTAATTTAGCTTTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGtt                                                                                                                         <  2:425558/150‑1 (MQ=255)                             gcgcCGCTTTCTTAGGATGGTTCACCCCTTAAGTTAGCCCTAAGGGGAT‑CACCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTtctc                                                                                                                            <  1:769890/146‑1 (MQ=255)                             gCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGt                                                                                                                          <  2:899253/148‑1 (MQ=255)                               gTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTAcc                                                                                                                      >  2:543489/1‑150 (MQ=255)                                 cGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAgc                                                                                                                   >  2:367818/1‑151 (MQ=255)                                  gctttcttcAGATGGTTCACCCCTAAAGTTAGCGCTTATGGGAT‑CCCTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAg                                                                                                                    <  1:873419/146‑1 (MQ=255)                                    aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGcgcc                                                                                                                >  2:368856/1‑151 (MQ=255)                                    aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGcgcc                                                                                                                >  2:495356/1‑151 (MQ=255)                                     ttcttcAGATGTTTCACCCCTTAATTTAGCTCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAgc                                                                                                                   <  2:1145695/147‑1 (MQ=255)                                     ttcttcAGATGGTTCAACCCTTAATTTAGCCTTTAGGGGAT‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAgcg                                                                                                                  <  2:939612/148‑1 (MQ=255)                                     ttcttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGcgccg                                                                                                               >  2:115613/1‑151 (MQ=255)                                           aGATGTTTCACCCCTTAATTTAGCGCTTATGGAAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCgccgcc                                                                                                             <  1:1187938/147‑1 (MQ=255)                                           aGATGGTTCACCCCTTAATTAAGCGCTTATGGGAT‑CCCTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCa                                                                                                            <  2:108576/148‑1 (MQ=255)                                           aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACt                                                                                                          >  2:792825/1‑150 (MQ=255)                                             aTGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTAcg                                                                                                       >  1:427874/1‑151 (MQ=255)                                                gTTCCACCCTTAAAGTAGCCCTTTTGGGATCCCCCCCCCGCCGTTGCTCTTCCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTAct                                                                                                       <  2:414464/149‑2 (MQ=255)                                                 ttCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTAcggcgg                                                                                                   >  2:1170379/1‑151 (MQ=255)                                                      cccTTAATTTAGCTTTTAGGGGCC‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGa                                                                                                <  2:536152/149‑1 (MQ=255)                                                      cccTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTTCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGa                                                                                                >  2:938330/1‑149 (MQ=255)                                                      cccTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTAcggcgg                                                                                                   >  1:1010138/1‑146 (MQ=255)                                                       ccTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATa                                                                                              >  2:1330168/1‑150 (MQ=255)                                                         ttaatttggcttttAGGGGAC‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATAc                                                                                             <  1:1080647/137‑1 (MQ=255)                                                         ttAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATAc                                                                                             >  2:845647/1‑149 (MQ=255)                                                              tgggcgtTTATGGACC‑CACCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCTGCCACTACGGCGGTGATACAGATg                                                                                        <  1:205536/146‑1 (MQ=255)                                                                aGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATAAAGATGACg                                                                                     >  2:811806/1‑150 (MQ=255)                                                                  cgcTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGGTACAGATGACg                                                                                     >  2:1407133/1‑148 (MQ=255)                                                                  cctttATGGAAT‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACTTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGAt                                                                                   <  1:207273/147‑1 (MQ=255)                                                                   gcTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATc                                                                                  >  2:1404974/1‑150 (MQ=255)                                                                     ttAGGGGAC‑CCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCa                                                                                 <  1:1324895/149‑1 (MQ=255)                                                                      ttagggaacccccccccGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGAt                                                                                   <  1:503554/134‑1 (MQ=255)                                                                      tATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAggg                                                                              >  2:865061/1‑151 (MQ=255)                                                                       tgtggaacccccccccGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCa                                                                                 <  1:1473024/136‑1 (MQ=255)                                                                           ggacccccccccGCCGTTGCTCTTCCTCGGATTCGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGAc                                                                          <  1:989553/143‑1 (MQ=255)                                                                                 cTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAatcat                                                                    >  1:1234775/1‑151 (MQ=255)                                                                                  tCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAatca                                                                     >  1:209797/1‑149 (MQ=255)                                                                                  cccccGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAatcatc                                                                   <  2:895174/150‑1 (MQ=255)                                                                                    cccGCCGTTGCTCTTACTCGTTTTCGTAAGCCGTAAAAACAGCATCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACa                                                                         <  1:1191640/143‑1 (MQ=255)                                                                                      cgccgTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACCACTGACGAACAAGGCGACCATCATCTcc                                                                >  2:988377/1‑150 (MQ=255)                                                                                      cgccgTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGcc                                                                >  2:1160009/1‑150 (MQ=255)                                                                                      cgccgTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGcc                                                                >  1:552389/1‑150 (MQ=255)                                                                                        ccgTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCCCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCtt                                                              <  1:666440/150‑1 (MQ=255)                                                                                           ttGCTCTTCCTGGGATTCGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑At                                                           <  2:376165/149‑1 (MQ=255)                                                                                           ttGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATTGCGATCAGGGCGAGAATCATCGCCTT‑Atg                                                          >  1:68099/1‑150 (MQ=255)                                                                                           tcgCTCTTCCTCGTATCCGTAAGCCGTAAAAACACCACCCGCCGCCTGGCCAGTGCGGACGTAACCCTCACAGGGGTATTTTCTCGTTACCGGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCtt                                                              <  2:711061/145‑1 (MQ=255)                                                                                               tctTACTCGGATTCGTAACCCGTGAAAACAGCACCCCCCGTCTGGCCAGTCCGGACGTGACCCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑Atgct                                                        <  1:729009/148‑1 (MQ=255)                                                                                                  tACTCGGTTTCGTAAGCCGTAAAAACAGCAACCTCCGTCTGGCCAGTCCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATgctgct                                                     <  1:316491/148‑1 (MQ=255)                                                                                                   aCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCa                                                  >  1:754371/1‑150 (MQ=255)                                                                                                    cTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCAt                                                 <  1:593428/150‑1 (MQ=255)                                                                                                      cGGATTCGTAGGCCGTAAAAACAGCAACCCCCGTCTGGCCAGTTCGGACGTGAACCCCATAGATGTGTTTTCTCGTTACCCGCGCCGCCACTACGGCGGTGATACAGATGACGAGCAGGGCGACAATCATCGCCTT‑a                                                            <  1:204663/137‑1 (MQ=255)                                                                                                      cGGATTCGTAAGCCGTGAAAACAGCACCCCCCGTCTGGCCAGTTCGGACGTGACCCTCACAGAGGTCTTTTCTCTTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTg                                               <  2:1031927/150‑1 (MQ=255)                                                                                                       ggATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGc                                              >  1:727979/1‑150 (MQ=255)                                                                                                           tCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGctctc                                          <  1:51499/150‑1 (MQ=255)                                                                                                            cGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGctctct                                         <  1:94063/150‑1 (MQ=255)                                                                                                               aaGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCCCAGAGGTCTTTTCTCTTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCtcttc                                       <  2:15473/149‑1 (MQ=255)                                                                                                               aaGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGGGACAATCATCGCCCT‑ATGCTGCTTCATTGCTCtctt                                        >  2:1457057/1‑148 (MQ=255)                                                                                                               aaGCCGTAAAACCAGCACCCCCCGTCTGGCCAGTTCGGCCGTGAACCTCACAGAGGTCTTTTCCCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTtctc                                     <  1:1423424/151‑1 (MQ=255)                                                                                                                aGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGGGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTcc                                    >  2:100219/1‑151 (MQ=255)                                                                                                                 gCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGGTTAAGGCGACAACAATCGCCTT‑TTGCTGCTTTAATGcccttctttcc                                    >  2:641871/1‑143 (MQ=255)                                                                                                                 gCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGAACAGGGCGACAATCATCGCCTT‑TTGCTGCTTCATTGCTCTCTTCTcc                                    >  2:978124/1‑150 (MQ=255)                                                                                                                  ccGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCtt                                  <  1:253119/151‑1 (MQ=255)                                                                                                                  ccGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCtt                                  <  1:900502/151‑1 (MQ=255)                                                                                                                      gAAAACAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGGTCAGGGCGACAATCATCGCCTT‑ATTCTGCTTCATTGCTCTCTTCTCCTTGAcc                              >  1:1355810/1‑151 (MQ=255)                                                                                                                       aaaaCAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGAcc                              >  1:597448/1‑150 (MQ=255)                                                                                                                        aaaCAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTTTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCtt                            <  1:1330276/151‑1 (MQ=255)                                                                                                                        aaaCAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCtt                            <  1:777206/151‑1 (MQ=255)                                                                                                                        aaaCAGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACCAACAGGGCGGCAATCATTGCCTTCTTACTG‑TTTATTGCTCTCTTTTTCTTGAcc                              >  1:34975/1‑149 (MQ=255)                                                                                                                         aaCAGCACCCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTa                           <  1:1170475/151‑1 (MQ=255)                                                                                                                           cAGCACCCCCCGCCGGGCCAGTTCGAACGTGACCCCCACAGAGGTCTTTCCTCTTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACg                         <  2:52619/151‑1 (MQ=255)                                                                                                                            aGCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTTCgg                        >  2:747179/1‑151 (MQ=255)                                                                                                                             gCAACCTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACg                         <  1:206301/149‑1 (MQ=255)                                                                                                                               acccTCCGTCTGGCCAGTTCGGACGTGACCTGCACAGAGGTCTTTTCTCTTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTTACCTTACGGTc                      <  2:1027849/148‑1 (MQ=37)                                                                                                                                 ccTCCGTCTGGCCAGTTCGGACGTGAACCTCACAGAGGTTTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTc                      <  2:1010680/148‑1 (MQ=255)                                                                                                                                    ccGTCTGGCCAGTTCGGACGTGACCCTCACAGAGGTTTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAag                <  2:1030543/151‑1 (MQ=255)                                                                                                                                          ggCCAGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCAc          >  2:690772/1‑151 (MQ=255)                                                                                                                                              aGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTAAGGGCAGTAAGAGGCAccct       >  1:1242283/1‑150 (MQ=255)                                                                                                                                              aGTTCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACAAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCActct       <  1:115630/150‑1 (MQ=255)                                                                                                                                                ttCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTAc     >  2:475693/1‑150 (MQ=255)                                                                                                                                                ttCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTAc     >  2:938071/1‑150 (MQ=255)                                                                                                                                                ttCGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGGGATACAGATGACGGTCTGGGCGACAATCACCGCCTT‑ATGCTGCTTCATTTCTTTCTTCTCCTTGACCTTACGGTCAGTAAGGGGCAc          >  2:333384/1‑145 (MQ=255)                                                                                                                                                  cGGACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGAAACAGATTACGATCAGGGCGACAAATATCGCCTT‑ATGCTGCCTCATTGCTCTCTTCCCCCTGACCTTACGGTCAGTAAGAGGCACTCTAc     >  1:773161/1‑148 (MQ=255)                                                                                                                                                   ggACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACAtg  <  2:788842/150‑1 (MQ=255)                                                                                                                                                   ggACGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACAtg  <  1:1179535/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAT‑CACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTT‑ATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATG  >  NC_000913/16656‑16950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑