Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,136,817	T→C	Q569R (CAA→CGA)	gss ←	glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,136,817	0	T	C	100.0%	348.7 / NA	93	Q569R (CAA→CGA)	gss	glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/53);  total (40/53)

TCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACAG  >  NC_000913/3136671‑3136964                                                                                                                                                   |                                                                                                                                                    tCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTg                                                                                                                                                 >  1:748875/1‑151 (MQ=255)    ccacAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGAt                                                                                                                                               >  1:310780/1‑150 (MQ=255)     cacaATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAg                                                                                                                                             <  2:76480/151‑1 (MQ=255)       caATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGa                                                                                                                                            <  1:1278960/150‑1 (MQ=255)        aaTTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATg                                                                                                                                          >  2:1068887/1‑151 (MQ=255)          ttAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGtt                                                                                                                                        >  2:1431876/1‑151 (MQ=255)           tAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGttt                                                                                                                                       >  2:37364/1‑151 (MQ=255)           tAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGttt                                                                                                                                       >  1:1004097/1‑151 (MQ=255)           tAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGttt                                                                                                                                       >  2:710513/1‑151 (MQ=255)            aaCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGtttt                                                                                                                                      <  2:333192/151‑1 (MQ=255)              cGGATCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTc                                                                                                                                    >  2:755892/1‑151 (MQ=255)               ggTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCTCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCt                                                                                                                                   >  1:1073636/1‑151 (MQ=255)               ggTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCt                                                                                                                                   <  1:1445349/151‑1 (MQ=255)                    aaTATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCg                                                                                                                              <  1:213252/151‑1 (MQ=255)                    aaTATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCg                                                                                                                              <  1:518670/151‑1 (MQ=255)                    aaTATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCg                                                                                                                              >  1:491660/1‑151 (MQ=255)                      tatCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGc                                                                                                                            >  1:684136/1‑151 (MQ=255)                      tatCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGc                                                                                                                            >  1:206368/1‑151 (MQ=255)                       atCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCa                                                                                                                           <  2:634242/151‑1 (MQ=255)                         cACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCaaa                                                                                                                         <  2:1357137/151‑1 (MQ=255)                             ctctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAAtt                                                                                                                       <  1:1147792/149‑1 (MQ=255)                               ctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACTGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTAcc                                                                                                                   >  1:1468744/1‑151 (MQ=255)                               ctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTa                                                                                                                     <  2:410591/149‑1 (MQ=255)                                 tttttGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTAcc                                                                                                                   <  2:161223/149‑1 (MQ=255)                                   tttGATGACCAGTCATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGACTGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTg                                                                                                               >  2:645256/1‑151 (MQ=255)                                    ttGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTgg                                                                                                              >  2:314396/1‑151 (MQ=255)                                     tgatgaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGt                                                                                                             >  2:1094035/1‑151 (MQ=255)                                     tgatgaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGt                                                                                                             >  1:339343/1‑151 (MQ=255)                                         gaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGtttt                                                                                                          <  2:737821/150‑1 (MQ=255)                                            cAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTcc                                                                                                      >  1:1471353/1‑151 (MQ=255)                                             aGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTcc                                                                                                      >  1:800401/1‑150 (MQ=255)                                              gTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAg                                                                                                    <  2:1083209/151‑1 (MQ=255)                                               tGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGc                                                                                                   >  2:280402/1‑151 (MQ=255)                                                    catcaCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACctc                                                                                              <  1:989934/151‑1 (MQ=255)                                                      tcaCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCtt                                                                                            >  2:300713/1‑151 (MQ=255)                                                         ccGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCat                                                                                         <  2:518370/151‑1 (MQ=255)                                                         ccGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCAGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCa                                                                                          <  1:404389/150‑1 (MQ=255)                                                          cgcgCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCatg                                                                                        >  2:988271/1‑151 (MQ=255)                                                             gcAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCatgatg                                                                                     <  2:1306168/151‑1 (MQ=255)                                                               aaaCACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGc                                                                                   >  2:452790/1‑151 (MQ=255)                                                               aaaCACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGc                                                                                   >  1:1209112/1‑151 (MQ=255)                                                               aaaCACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGc                                                                                   <  1:1125449/151‑1 (MQ=255)                                                                     gTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGa                                                                             <  2:427589/151‑1 (MQ=255)                                                                      tCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACg                                                                              >  2:998841/1‑149 (MQ=255)                                                                         cACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTc                                                                         <  2:661969/151‑1 (MQ=255)                                                                         cACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTc                                                                         <  1:36018/151‑1 (MQ=255)                                                                            cGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGat                                                                      <  2:766469/151‑1 (MQ=255)                                                                             gTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGat                                                                      >  1:715523/1‑150 (MQ=255)                                                                              tAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGatat                                                                    >  2:1034291/1‑151 (MQ=255)                                                                                 ttGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATtgc                                                                 >  1:356882/1‑151 (MQ=255)                                                                                 ttGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTAGATATtg                                                                  <  1:904267/150‑1 (MQ=255)                                                                                   gccgccAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATtgctg                                                               <  1:119809/151‑1 (MQ=255)                                                                                   gccgccAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATtgct                                                                <  1:811336/150‑1 (MQ=255)                                                                                        cAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCcac                                                           >  2:901928/1‑150 (MQ=255)                                                                                               gAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCg                                                   <  2:552196/151‑1 (MQ=255)                                                                                                 aGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGc                                                 >  2:1473086/1‑151 (MQ=255)                                                                                                 aGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGc                                                 <  2:206345/151‑1 (MQ=255)                                                                                                 aGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGc                                                 <  1:1300422/151‑1 (MQ=255)                                                                                                     aCATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGAt                                              <  2:565546/150‑1 (MQ=255)                                                                                                     aCATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATc                                             >  1:1280643/1‑151 (MQ=255)                                                                                                       aTACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCgg                                           >  1:1284448/1‑151 (MQ=255)                                                                                                             gAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTc                                       <  2:851394/149‑1 (MQ=255)                                                                                                             gAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCa                                      <  1:782872/150‑1 (MQ=255)                                                                                                             gAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCa                                      <  2:88505/150‑1 (MQ=255)                                                                                                              aaTGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACt                                    <  2:748798/151‑1 (MQ=255)                                                                                                              aaTGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACt                                    >  2:723106/1‑151 (MQ=255)                                                                                                               aTGTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTg                                   <  1:1315252/151‑1 (MQ=255)                                                                                                                 gTATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGc                                  <  1:1250191/150‑1 (MQ=255)                                                                                                                  tATTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGt                                >  1:214313/1‑151 (MQ=255)                                                                                                                   aTTTACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTa                               <  1:1319141/151‑1 (MQ=255)                                                                                                                      tACCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAAcc                            >  2:255790/1‑151 (MQ=255)                                                                                                                       aCCGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCt                           <  2:660843/151‑1 (MQ=255)                                                                                                                        ccGTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCt                           <  1:1068988/150‑1 (MQ=255)                                                                                                                          gTCCACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTGACCTGtt                        <  2:1314784/151‑1 (MQ=255)                                                                                                                            ccACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGtttt                      <  2:1351580/151‑1 (MQ=255)                                                                                                                            ccACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGtttt                      >  1:1251981/1‑151 (MQ=255)                                                                                                                             cACTTTCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTc                     <  1:255815/151‑1 (MQ=255)                                                                                                                                tttCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCAcc                  >  1:646385/1‑151 (MQ=255)                                                                                                                                 ttCGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCAcc                  >  2:939203/1‑150 (MQ=255)                                                                                                                                   cGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGt               <  1:739705/151‑1 (MQ=255)                                                                                                                                   cGGCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTCTCACCAg                <  1:1094133/150‑1 (MQ=255)                                                                                                                                    ggCAAACACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGtt              <  1:314433/151‑1 (MQ=255)                                                                                                                                       aaaCACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTtcat           <  1:1431981/151‑1 (MQ=255)                                                                                                                                       aaaCACCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTtcat           >  1:490565/1‑151 (MQ=255)                                                                                                                                         acacCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTtcatca         <  2:339301/151‑1 (MQ=255)                                                                                                                                         acacCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTtcatca         <  1:357306/151‑1 (MQ=255)                                                                                                                                         acacCACAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTtcatca         <  1:718946/151‑1 (MQ=255)                                                                                                                                          caccacAGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTcatcat        <  2:1036814/151‑1 (MQ=255)                                                                                                                                             cacaGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTaa     <  1:194764/151‑1 (MQ=255)                                                                                                                                             cacaGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTaa     <  1:710592/151‑1 (MQ=255)                                                                                                                                             cacaGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTa      >  1:1178324/1‑150 (MQ=255)                                                                                                                                               caGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACa   <  2:578018/151‑1 (MQ=255)                                                                                                                                                aGTCGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACAg  <  2:780728/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACAG  >  NC_000913/3136671‑3136964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑