Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,550	C→T	G423G (GGC→GGT)	garD →	D‑galactarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,550	0	C	T	100.0%	389.1 / NA	95	G423G (GGC→GGT)	garD	D‑galactarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/51);  total (44/51)

CGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCG  >  NC_000913/3276403‑3276697                                                                                                                                                    |                                                                                                                                                    cGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACg                                                                                                                                                  <  1:182216/151‑1 (MQ=255) cGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACg                                                                                                                                                  <  1:1308116/151‑1 (MQ=255)      tGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAAc                                                                                                                                              >  2:1326224/1‑150 (MQ=255)      tGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACACCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAAc                                                                                                                                              >  1:140679/1‑150 (MQ=255)       ggCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAg                                                                                                                                            <  1:1409205/151‑1 (MQ=255)        gCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAgg                                                                                                                                           >  2:148670/1‑151 (MQ=255)          aaaCGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTg                                                                                                                                         >  1:827367/1‑151 (MQ=255)          aaaCGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTg                                                                                                                                         <  2:473921/151‑1 (MQ=255)              gTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCtt                                                                                                                                     >  2:1242874/1‑151 (MQ=255)              gTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCtt                                                                                                                                     >  1:1232750/1‑151 (MQ=255)              gTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCtt                                                                                                                                     <  2:218363/151‑1 (MQ=255)                ggTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTc                                                                                                                                    <  1:1390630/150‑1 (MQ=255)                ggTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTc                                                                                                                                    <  1:99717/150‑1 (MQ=255)                ggTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTc                                                                                                                                    <  1:451333/150‑1 (MQ=255)                   agagAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGt                                                                                                                                <  2:266944/151‑1 (MQ=255)                     agaAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTAt                                                                                                                              >  1:1306882/1‑151 (MQ=255)                         ggCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATcaca                                                                                                                          <  1:277755/151‑1 (MQ=255)                         ggCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATcaca                                                                                                                          <  2:1460927/151‑1 (MQ=255)                          gCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATcac                                                                                                                           >  2:1463816/1‑149 (MQ=255)                              tCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTTCGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTg                                                                                                                       <  2:1094697/149‑1 (MQ=255)                                   tCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAgtgt                                                                                                                >  1:464754/1‑151 (MQ=255)                                    ccATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGtt                                                                                                               <  2:884302/151‑1 (MQ=255)                                     cATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGttt                                                                                                              <  1:78916/151‑1 (MQ=255)                                         gCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGa                                                                                                          <  1:852229/151‑1 (MQ=255)                                         gCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGa                                                                                                          <  2:737188/151‑1 (MQ=255)                                          cTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                                                                                                         >  1:143313/1‑151 (MQ=255)                                          cTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                                                                                                         >  1:892038/1‑151 (MQ=255)                                          cTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                                                                                                         >  1:729822/1‑151 (MQ=255)                                            aaaTCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAcc                                                                                                        <  1:1417393/150‑1 (MQ=255)                                            aaaTCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCg                                                                                                       >  1:1429488/1‑151 (MQ=255)                                              aTCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCGGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGt                                                                                                     <  2:709865/151‑1 (MQ=255)                                              aTCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGt                                                                                                     <  1:1116113/151‑1 (MQ=255)                                               tCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTc                                                                                                    >  1:1385562/1‑151 (MQ=255)                                                cGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCg                                                                                                   >  1:799374/1‑151 (MQ=255)                                                 gggTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCg                                                                                                   >  2:217539/1‑150 (MQ=255)                                                     aaaaGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTa                                                                                              <  2:755362/151‑1 (MQ=255)                                                      aaaGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTAc                                                                                             >  1:302609/1‑151 (MQ=255)                                                       aaGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACg                                                                                            <  1:237101/151‑1 (MQ=255)                                                          cgcAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCg                                                                                         <  2:1182600/151‑1 (MQ=255)                                                           gcAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGt                                                                                        >  1:664349/1‑151 (MQ=255)                                                            cAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGt                                                                                        >  2:519852/1‑150 (MQ=255)                                                            cAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGt                                                                                        >  2:273846/1‑150 (MQ=255)                                                             aaTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTAc                                                                                      >  2:21390/1‑151 (MQ=255)                                                              aTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACg                                                                                     <  2:319304/151‑1 (MQ=255)                                                                tgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACgg                                                                                    <  1:291831/150‑1 (MQ=255)                                                                  ttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCt                                                                                 >  1:1291071/1‑151 (MQ=255)                                                                   tgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCt                                                                                 <  1:201701/150‑1 (MQ=255)                                                                   tgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTg                                                                                >  2:1377224/1‑151 (MQ=255)                                                                   tgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTg                                                                                >  2:355287/1‑151 (MQ=255)                                                                    gAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTg                                                                                <  2:1064236/150‑1 (MQ=255)                                                                        tGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGc                                                                           >  2:493427/1‑151 (MQ=255)                                                                         gCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCg                                                                          <  2:1429383/151‑1 (MQ=255)                                                                           tGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGt                                                                        >  1:837776/1‑151 (MQ=255)                                                                             tCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTAc                                                                      >  2:933425/1‑151 (MQ=255)                                                                                 ccGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGt                                                                  >  2:488841/1‑151 (MQ=255)                                                                                 ccGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGt                                                                  <  1:1338229/151‑1 (MQ=255)                                                                                  cGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTc                                                                 >  2:55807/1‑151 (MQ=255)                                                                                    gTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCAt                                                               <  2:859488/151‑1 (MQ=255)                                                                                         cGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTaaa                                                           >  1:533244/1‑150 (MQ=255)                                                                                           cccGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAAt                                                         >  2:1199993/1‑150 (MQ=255)                                                                                           cccGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATg                                                        <  1:1328600/151‑1 (MQ=255)                                                                                               aCTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCa                                                     >  1:995813/1‑150 (MQ=255)                                                                                                cTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAAc                                                   >  1:175553/1‑151 (MQ=255)                                                                                                 tAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAAcc                                                  >  1:356731/1‑151 (MQ=255)                                                                                                   aaCGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCg                                                <  2:82152/151‑1 (MQ=255)                                                                                                       cgGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCAcc                                            <  2:934697/151‑1 (MQ=255)                                                                                                         gATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGa                                          <  1:1082388/151‑1 (MQ=255)                                                                                                          aTTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCg                                           >  2:1134025/1‑149 (MQ=255)                                                                                                             aaTTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCt                                       <  2:895013/150‑1 (MQ=255)                                                                                                             aaTTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCt                                       <  1:975308/150‑1 (MQ=255)                                                                                                             aaTTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTg                                      <  2:371533/151‑1 (MQ=255)                                                                                                                ttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGCCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCg                                   >  2:1321901/1‑151 (MQ=255)                                                                                                                 tACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGTTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGc                                    <  1:411332/149‑1 (MQ=255)                                                                                                                 tACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCg                                   <  1:858688/150‑1 (MQ=255)                                                                                                                  aCGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGa                                  <  1:1257154/150‑1 (MQ=255)                                                                                                                  aCGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGa                                  >  1:719017/1‑150 (MQ=255)                                                                                                                   cgccgcGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAAc                                >  1:215601/1‑151 (MQ=255)                                                                                                                   cgccgcGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAAc                                >  2:726695/1‑151 (MQ=255)                                                                                                                     ccgcGACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCg                              >  2:381279/1‑151 (MQ=255)                                                                                                                        cgACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTg                           >  2:685816/1‑151 (MQ=255)                                                                                                                         gACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTgg                          <  1:21392/151‑1 (MQ=255)                                                                                                                         gACGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTgg                          <  2:694692/151‑1 (MQ=255)                                                                                                                          aCGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGt                         <  2:1358222/151‑1 (MQ=255)                                                                                                                           cGCCAGCCAGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGtt                        <  2:1291339/151‑1 (MQ=255)                                                                                                                            gccagccaGCGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGttt                       <  1:55814/151‑1 (MQ=255)                                                                                                                                gccagcGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGAtt                   <  2:302574/151‑1 (MQ=255)                                                                                                                                  cagcGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGAttt                  >  2:1016784/1‑150 (MQ=255)                                                                                                                                  cagcGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                 <  1:1435303/151‑1 (MQ=255)                                                                                                                                  cagcGATTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                 <  1:379764/151‑1 (MQ=255)                                                                                                                                       aTTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATgg             >  1:300473/1‑150 (MQ=255)                                                                                                                                       aTTTTGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGa            <  2:1381531/151‑1 (MQ=255)                                                                                                                                         tttGTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGat           <  2:140659/150‑1 (MQ=255)                                                                                                                                            gTCTGTGGTACGCACCAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCGGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGAGATTa       <  2:827287/151‑1 (MQ=255)                                                                                                                                            gTCTGTGGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATAtt        <  2:799296/150‑1 (MQ=255)                                                                                                                                                 tgGTACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCg  >  1:1246324/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCG  >  NC_000913/3276403‑3276697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑