Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,560	A→G	T73A (ACG→GCG)	gspJ →	putative general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,560	0	A	G	100.0%	279.9 / NA	72	T73A (ACG→GCG)	gspJ	putative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/29);  total (43/29)

TAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTG  >  NC_000913/3462412‑3462707                                                                                                                                                     |                                                                                                                                                    tAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCg                                                                                                                                                   >  1:353008/1‑151 (MQ=255)    cGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGa                                                                                                                                                 <  2:714759/150‑1 (MQ=255)    cGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGAc                                                                                                                                                >  2:137491/1‑151 (MQ=255)      tGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACa                                                                                                                                               >  1:619580/1‑150 (MQ=255)       gCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACaa                                                                                                                                              >  2:441625/1‑150 (MQ=255)          ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAAt                                                                                                                                           <  2:352731/150‑1 (MQ=255)          ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATc                                                                                                                                          <  2:779376/151‑1 (MQ=255)            cGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCAt                                                                                                                                        <  2:945276/151‑1 (MQ=255)             gTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATg                                                                                                                                       <  2:1287858/151‑1 (MQ=255)              tttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATgg                                                                                                                                      >  2:58235/1‑151 (MQ=255)               ttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATgg                                                                                                                                      <  2:110671/150‑1 (MQ=255)                tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGgt                                                                                                                                     <  1:360329/150‑1 (MQ=255)                 aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGgtgt                                                                                                                                   >  2:504889/1‑151 (MQ=255)                    aTTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTgg                                                                                                                                <  2:1416946/151‑1 (MQ=255)                      tttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGt                                                                                                                              >  2:1468691/1‑151 (MQ=255)                             cAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGaagaag                                                                                                                       <  2:475303/151‑1 (MQ=255)                                gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                                                                    <  1:36811/151‑1 (MQ=255)                                gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                                                                    <  2:668116/151‑1 (MQ=255)                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTAtt                                                                                                                  >  1:126281/1‑151 (MQ=255)                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTAtt                                                                                                                  >  1:1410098/1‑151 (MQ=255)                                   tCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTAttt                                                                                                                 >  2:774641/1‑151 (MQ=255)                                    ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTAtttt                                                                                                                >  1:798092/1‑151 (MQ=255)                                    ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTAttt                                                                                                                 <  1:788031/150‑1 (MQ=255)                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                                                                             >  1:401109/1‑151 (MQ=255)                                               cAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCaa                                                                                                      <  2:167923/150‑1 (MQ=255)                                                aaCGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCaa                                                                                                      >  2:581656/1‑149 (MQ=255)                                                aaCGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAg                                                                                                    <  2:120367/151‑1 (MQ=255)                                                      cacaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgcg                                                                                               <  1:630575/150‑1 (MQ=255)                                                      cacaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgcg                                                                                               >  2:1442797/1‑150 (MQ=255)                                                            aagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCt                                                                                        <  2:1063211/151‑1 (MQ=255)                                                                    cagcagTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcctc                                                                                >  2:927821/1‑151 (MQ=255)                                                                        agTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCtctc                                                                              >  1:88000/1‑149 (MQ=255)                                                                           tCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTg                                                                          >  2:948014/1‑150 (MQ=255)                                                                           tCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTg                                                                          >  2:1206241/1‑150 (MQ=255)                                                                              aTCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGc                                                                      >  2:632565/1‑151 (MQ=255)                                                                               tCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGc                                                                      >  2:667409/1‑150 (MQ=255)                                                                                cAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac                                                                    >  2:176551/1‑151 (MQ=255)                                                                                 aGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac                                                                    >  2:1337633/1‑150 (MQ=255)                                                                                        gcgcACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCtt                                                             >  2:601837/1‑150 (MQ=255)                                                                                            accatcaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTc                                                         >  2:510931/1‑150 (MQ=255)                                                                                            accatcaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTc                                                         >  1:593784/1‑150 (MQ=255)                                                                                                   ccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTc                                                  <  2:1239686/150‑1 (MQ=255)                                                                                                   ccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTc                                                  >  2:1086061/1‑150 (MQ=255)                                                                                                   ccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTc                                                  >  1:85527/1‑150 (MQ=255)                                                                                                    catTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCAc                                                >  2:429146/1‑151 (MQ=255)                                                                                                      ttCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTg                                              >  2:1356561/1‑151 (MQ=255)                                                                                                         ttGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGt                                            >  1:36153/1‑150 (MQ=255)                                                                                                             tAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTg                                       >  1:149710/1‑151 (MQ=255)                                                                                                             tAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTg                                       >  2:1230401/1‑151 (MQ=255)                                                                                                              aaCGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTg                                       <  1:141151/150‑1 (MQ=255)                                                                                                                 gATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGa                                    >  2:100925/1‑150 (MQ=255)                                                                                                                  aTCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGaa                                   >  1:253845/1‑150 (MQ=255)                                                                                                                   tctcCTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAAt                                 >  1:737399/1‑151 (MQ=255)                                                                                                                       cTGCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCAGTGTTCACTGGTATCTGCGaaa                                  <  2:401053/146‑1 (MQ=255)                                                                                                                         gCAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACg                           >  1:248072/1‑151 (MQ=255)                                                                                                                          cAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGc                          <  2:337257/151‑1 (MQ=255)                                                                                                                          cAACTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGc                          >  2:1230246/1‑151 (MQ=255)                                                                                                                           aaCTGGTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGc                          >  2:523573/1‑150 (MQ=255)                                                                                                                               ggTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGctct                       <  2:85516/149‑1 (MQ=255)                                                                                                                               ggTCGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTAc                     <  1:250566/151‑1 (MQ=255)                                                                                                                                  cGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCg                   <  1:504946/150‑1 (MQ=255)                                                                                                                                  cGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGt                  >  1:1276641/1‑151 (MQ=255)                                                                                                                                  cGCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGt                  <  2:1244827/151‑1 (MQ=255)                                                                                                                                   gCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTg                 <  2:477345/151‑1 (MQ=255)                                                                                                                                   gCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTg                 >  2:1379678/1‑151 (MQ=255)                                                                                                                                   gCCCGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTg                 >  2:920507/1‑151 (MQ=255)                                                                                                                                      cGGAGGAATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTg              <  1:614388/151‑1 (MQ=255)                                                                                                                                        gaggaATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGtt            <  2:126264/151‑1 (MQ=255)                                                                                                                                          ggaATCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCg          >  2:511165/1‑151 (MQ=255)                                                                                                                                            aaTCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGt         >  2:153134/1‑150 (MQ=255)                                                                                                                                            aaTCGGAGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTa        <  2:1409976/151‑1 (MQ=255)                                                                                                                                                  aGTGCGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCtg  <  2:36148/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTG  >  NC_000913/3462412‑3462707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑