Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 304,834 A→G R124R (CGT→CGC ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913304,8340AG98.8% 286.1 / ‑3.7 80R124R (CGT→CGCecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (42/37);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

TTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTC  >  NC_000913/304688‑304979
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ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCg                                                                                                                                                 >  2:397318/1‑149 (MQ=255)
   ccAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTgg                                                                                                                                            <  1:316667/151‑1 (MQ=255)
       tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGAc                                                                                                                                        >  2:373382/1‑151 (MQ=255)
             ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTcgcg                                                                                                                                  >  2:1374676/1‑151 (MQ=255)
              tacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTcgc                                                                                                                                   <  1:654536/149‑1 (MQ=255)
                 cGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAAt                                                                                                                              >  1:606738/1‑151 (MQ=255)
                  gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             <  2:930799/151‑1 (MQ=255)
                  gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             >  1:588041/1‑151 (MQ=255)
                  gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             >  1:159838/1‑151 (MQ=255)
                   ttCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             >  1:1025072/1‑150 (MQ=255)
                    tCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             >  1:291026/1‑149 (MQ=255)
                     ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATg                                                                                                                             >  1:596639/1‑148 (MQ=255)
                      cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGAc                                                                                                                         >  1:323207/1‑151 (MQ=255)
                       tGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGAcc                                                                                                                        >  1:201165/1‑151 (MQ=255)
                             cTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGgt                                                                                                                    >  1:642465/1‑149 (MQ=255)
                               tGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGgtag                                                                                                                  <  2:1368956/149‑1 (MQ=255)
                               tGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGgtag                                                                                                                  <  2:702884/149‑1 (MQ=255)
                                gTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGgtagt                                                                                                                 >  2:19416/1‑149 (MQ=255)
                                   gAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgcgc                                                                                                            >  2:486475/1‑151 (MQ=255)
                                   gAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgcgc                                                                                                            >  1:932751/1‑151 (MQ=255)
                                      ggACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTcg                                                                                                         >  1:1417946/1‑151 (MQ=255)
                                       gACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTcgc                                                                                                        >  2:417612/1‑151 (MQ=255)
                                             ttAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTa                                                                                                   <  1:1083848/150‑1 (MQ=255)
                                               aaaCTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATcc                                                                                                >  2:67465/1‑151 (MQ=255)
                                                aaCTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATccc                                                                                               >  1:484047/1‑151 (MQ=255)
                                                  cTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCg                                                                                              <  2:1468113/150‑1 (MQ=255)
                                                    gAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTgg                                                                                           <  1:927829/151‑1 (MQ=255)
                                                    gAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTgg                                                                                           <  2:323167/151‑1 (MQ=255)
                                                    gAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTg                                                                                            <  1:1090796/150‑1 (MQ=255)
                                                     aaCCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTgg                                                                                           >  1:1024570/1‑150 (MQ=255)
                                                       ccTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGAcc                                                                                        >  2:1472886/1‑151 (MQ=255)
                                                         tGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                                                                      >  2:1309934/1‑151 (MQ=255)
                                                         tGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                                                                      >  1:1300498/1‑151 (MQ=255)
                                                          gACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCa                                                                                       <  1:691821/149‑1 (MQ=255)
                                                          gACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                                                                      <  1:937509/150‑1 (MQ=255)
                                                          gACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATg                                                                                     <  2:1337877/151‑1 (MQ=255)
                                                           aCGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATg                                                                                     >  2:518365/1‑150 (MQ=255)
                                                             gTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGAt                                                                                   >  1:1359647/1‑150 (MQ=255)
                                                             gTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATa                                                                                  <  2:1422212/151‑1 (MQ=255)
                                                               ggTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAg                                                                                 <  2:1351267/150‑1 (MQ=255)
                                                                gTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTa                                                                               <  2:660676/151‑1 (MQ=255)
                                                                  cGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaaa                                                                             <  2:558509/151‑1 (MQ=255)
                                                                       tacCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAttt                                                                         >  1:1194003/1‑150 (MQ=255)
                                                                        acCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                                                                       <  1:906196/151‑1 (MQ=255)
                                                                        acCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                                                                       <  1:223643/151‑1 (MQ=255)
                                                                         ccAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaaa                                                                      <  1:702444/151‑1 (MQ=255)
                                                                         ccAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTaa                                                                       <  1:935823/150‑1 (MQ=255)
                                                                           aGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAAt                                                                    >  1:576011/1‑151 (MQ=255)
                                                                            gAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATa                                                                   <  2:1428625/151‑1 (MQ=255)
                                                                             aaTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATAc                                                                  <  2:606666/151‑1 (MQ=255)
                                                                              aTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACt                                                                 >  1:1144532/1‑151 (MQ=255)
                                                                              aTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACt                                                                 <  2:1258431/151‑1 (MQ=255)
                                                                              aTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACt                                                                 >  1:876940/1‑151 (MQ=255)
                                                                                ggTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCg                                                               <  2:159817/151‑1 (MQ=255)
                                                                                   aTCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGct                                                             <  2:175241/150‑1 (MQ=255)
                                                                                      cATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCa                                                         <  1:618731/151‑1 (MQ=255)
                                                                                           ccaccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAg                                                     <  2:527772/150‑1 (MQ=255)
                                                                                            caccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc                                                   >  2:339166/1‑151 (MQ=255)
                                                                                              ccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTCTCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTg                                                 >  2:319739/1‑151 (MQ=255)
                                                                                               caccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGc                                                >  1:35335/1‑151 (MQ=255)
                                                                                                 ccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCa                                               >  1:607538/1‑150 (MQ=255)
                                                                                                  cGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAAc                                             >  2:71678/1‑151 (MQ=255)
                                                                                                  cGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAAc                                             >  1:238506/1‑151 (MQ=255)
                                                                                                      tCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGcc                                          >  1:1285208/1‑150 (MQ=255)
                                                                                                         gTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCt                                      >  2:629130/1‑151 (MQ=255)
                                                                                                           gCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTg                                     <  2:116640/150‑1 (MQ=255)
                                                                                                           gCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTg                                     <  1:914492/150‑1 (MQ=255)
                                                                                                                  aCGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCgggggg                             >  2:409811/1‑151 (MQ=255)
                                                                                                                  aCGCCGCCGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAATAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCgggggg                             <  2:364445/151‑1 (MQ=255)
                                                                                                                    gccgccGGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCg                           <  2:596568/151‑1 (MQ=255)
                                                                                                                     ccgccgGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGa                          <  1:457119/151‑1 (MQ=255)
                                                                                                                      cgccgGTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGa                          >  2:984995/1‑150 (MQ=255)
                                                                                                                          ggTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGc                     >  1:304924/1‑151 (MQ=255)
                                                                                                                           gTTGGGCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGc                     >  2:765939/1‑150 (MQ=255)
                                                                                                                              gggCTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGt                  <  1:1069998/150‑1 (MQ=255)
                                                                                                                                 cTGCGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCAc              >  2:368754/1‑151 (MQ=255)
                                                                                                                                    cGTCTTGTATGGTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCAt           <  2:874856/151‑1 (MQ=255)
                                                                                                                                    cGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCa            >  2:726352/1‑150 (MQ=255)
                                                                                                                                          gTATGGTTGCGAGTGGGGACCTCGGGAAATGAGACCCGGTAGTAGCGCTCCCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACACCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATcc         <  1:490887/147‑1 (MQ=255)
                                                                                                                                              ggTTGCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTc  <  2:1288690/150‑1 (MQ=255)
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TTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTC  >  NC_000913/304688‑304979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: