Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,605,363 A→G M83T (ATG→ACG)  yhhM ← DUF2500 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,605,3630AG100.0% 364.2 / NA 96M83T (ATG→ACG) yhhMDUF2500 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/38);  total (58/38)

TGGCAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCATTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTGCTGGAGCGGAGCCATAT  >  NC_000913/3605219‑3605510
                                                                                                                                                |                                                                                                                                                   
tggcAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTtcctc                                                                                                                                               >  2:738825/1‑151 (MQ=255)
 ggcAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTtcct                                                                                                                                                <  2:753720/149‑1 (MQ=255)
  gcAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCg                                                                                                                                             <  2:1080740/151‑1 (MQ=255)
   cAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGc                                                                                                                                            >  2:917045/1‑151 (MQ=255)
      aagaaATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCttt                                                                                                                                         <  1:1053445/151‑1 (MQ=255)
        gaaATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGc                                                                                                                                       <  1:1184602/151‑1 (MQ=255)
         aaaTTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCg                                                                                                                                      >  2:900936/1‑151 (MQ=255)
         aaaTTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCg                                                                                                                                      >  2:662704/1‑151 (MQ=255)
          aaTTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCgg                                                                                                                                     >  2:1325838/1‑151 (MQ=255)
           aTTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGt                                                                                                                                    >  1:1415298/1‑151 (MQ=255)
            tttAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGtt                                                                                                                                   >  2:1262938/1‑151 (MQ=255)
             ttAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGttt                                                                                                                                  >  2:514232/1‑151 (MQ=255)
             ttAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGttt                                                                                                                                  >  2:1443201/1‑151 (MQ=255)
             ttAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGGTCCTCCGCTTTGCGGgtt                                                                                                                                  >  1:277811/1‑151 (MQ=255)
             gtAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGttt                                                                                                                                  >  2:900573/2‑151 (MQ=255)
              tAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTg                                                                                                                                 >  1:89863/1‑151 (MQ=255)
              tAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTg                                                                                                                                 >  1:550356/1‑151 (MQ=255)
               aaaGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTg                                                                                                                                 >  2:1445412/1‑150 (MQ=255)
               aaaGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTg                                                                                                                                 >  1:387868/1‑150 (MQ=255)
               aaaGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTg                                                                                                                                 >  1:1023638/1‑150 (MQ=255)
                   ttAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCt                                                                                                                            >  1:1150188/1‑151 (MQ=255)
                   ttAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCt                                                                                                                            >  1:20468/1‑151 (MQ=255)
                     aaaaaaTAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCtt                                                                                                                           <  1:231357/150‑1 (MQ=255)
                     aaaaaaTAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCtt                                                                                                                           <  1:1330071/150‑1 (MQ=255)
                        aaaTAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCt                                                                                                                       >  2:175811/1‑151 (MQ=255)
                         aaTAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTc                                                                                                                      >  2:1170819/1‑151 (MQ=255)
                           tAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCAt                                                                                                                    >  1:828854/1‑151 (MQ=255)
                           tAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCAt                                                                                                                    >  1:661724/1‑151 (MQ=255)
                            aaCCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCAt                                                                                                                    <  1:367949/150‑1 (MQ=255)
                                ttATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGt                                                                                                               >  2:1151816/1‑151 (MQ=255)
                                 tATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGt                                                                                                               >  1:533792/1‑150 (MQ=255)
                                     gTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACt                                                                                                          >  1:441785/1‑151 (MQ=255)
                                     gTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACt                                                                                                          >  1:386863/1‑151 (MQ=255)
                                        cGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGt                                                                                                       >  1:1171422/1‑151 (MQ=255)
                                         gCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGtg                                                                                                      >  2:836806/1‑151 (MQ=255)
                                           cTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGtgcc                                                                                                    >  1:996831/1‑151 (MQ=255)
                                            tACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGtgcct                                                                                                   >  2:1424872/1‑151 (MQ=255)
                                             aCAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGtgcctg                                                                                                  <  1:976610/151‑1 (MQ=255)
                                               aaaGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGtgcctgcc                                                                                                >  2:279456/1‑151 (MQ=255)
                                                     gACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGt                                                                                           <  1:1394900/150‑1 (MQ=255)
                                                     gACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTc                                                                                          <  1:791757/151‑1 (MQ=255)
                                                          aGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTc                                                                                     <  2:359620/151‑1 (MQ=255)
                                                           gcgcGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTcc                                                                                    <  1:1447675/151‑1 (MQ=255)
                                                                ttCCTTTAAAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCctgctg                                                                               >  2:543094/1‑151 (MQ=255)
                                                                 tCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCctgctg                                                                               <  2:1328544/150‑1 (MQ=255)
                                                                  ccTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGgc                                                                             >  1:913076/1‑151 (MQ=255)
                                                                      tataGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGcgcg                                                                          <  2:1193324/150‑1 (MQ=255)
                                                                      tataGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGcgcg                                                                          <  2:1379184/150‑1 (MQ=255)
                                                                      tataGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGcgcg                                                                          <  1:165989/150‑1 (MQ=255)
                                                                       ataGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGaa                                                                        <  1:900659/151‑1 (MQ=255)
                                                                       ataGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGa                                                                         >  1:539929/1‑150 (MQ=255)
                                                                         aGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACg                                                                      >  1:229196/1‑151 (MQ=255)
                                                                           cTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGc                                                                    >  1:1427747/1‑151 (MQ=255)
                                                                            tCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCg                                                                   >  1:724836/1‑151 (MQ=255)
                                                                                cGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCg                                                               <  1:831869/151‑1 (MQ=255)
                                                                                cGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCg                                                               >  1:821855/1‑151 (MQ=255)
                                                                                cGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCg                                                               >  1:551702/1‑151 (MQ=255)
                                                                                 gttccTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCGTGCCTCATAGCTTATACTGATGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGt                                                              <  1:751548/148‑1 (MQ=255)
                                                                                 gTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGt                                                              >  1:132822/1‑151 (MQ=255)
                                                                                 gTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGt                                                              <  2:339399/151‑1 (MQ=255)
                                                                                  tACCTTTATCGCCCACTGTCAGGGCGTGGTATTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGtt                                                             >  2:1314687/1‑151 (MQ=255)
                                                                                  tACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGtt                                                             >  2:805968/1‑151 (MQ=255)
                                                                                    ccTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTaa                                                           <  2:1420412/151‑1 (MQ=255)
                                                                                    ccTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTa                                                            <  2:807445/150‑1 (MQ=255)
                                                                                     cTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAAt                                                          >  1:1386916/1‑151 (MQ=255)
                                                                                       ttATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAAt                                                          <  1:645101/149‑1 (MQ=255)
                                                                                       ttATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAAt                                                          <  1:512394/149‑1 (MQ=255)
                                                                                       ttATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATc                                                         <  2:89852/150‑1 (MQ=255)
                                                                                          tCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGtt                                                     >  1:980933/1‑151 (MQ=255)
                                                                                          tCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGtt                                                     >  1:1288319/1‑151 (MQ=255)
                                                                                            gCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGtttt                                                   <  1:796828/151‑1 (MQ=255)
                                                                                            gCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAAAGGCGATCGTTAATCGGtttt                                                   >  1:8826/1‑151 (MQ=255)
                                                                                               cACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTcc                                                >  2:829890/1‑151 (MQ=255)
                                                                                               cACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTcc                                                <  1:928948/151‑1 (MQ=255)
                                                                                                   gTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCt                                            <  2:522856/151‑1 (MQ=255)
                                                                                                     cAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCttg                                          <  2:1362229/151‑1 (MQ=255)
                                                                                                     cAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCtt                                           <  1:1356436/150‑1 (MQ=255)
                                                                                                        ggCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCttgttg                                       >  1:232223/1‑151 (MQ=255)
                                                                                                        ggCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCttgttg                                       <  1:279488/151‑1 (MQ=255)
                                                                                                          cGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCt                                     <  2:550292/151‑1 (MQ=255)
                                                                                                           gTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCt                                     >  2:375734/1‑150 (MQ=255)
                                                                                                           gTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTc                                    <  1:732771/151‑1 (MQ=255)
                                                                                                               tACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcacc                                 >  2:293905/1‑150 (MQ=255)
                                                                                                               tACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcacc                                 >  1:394669/1‑150 (MQ=255)
                                                                                                                attgctgGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcaccac                               <  1:1314794/149‑1 (MQ=255)
                                                                                                                aCTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcaccac                               >  2:324923/1‑151 (MQ=255)
                                                                                                                aCTGCTGGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcaccac                               >  1:1023035/1‑151 (MQ=255)
                                                                                                                   gctgGGCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTcaccaccac                            <  1:738904/151‑1 (MQ=255)
                                                                                                                        gCGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCGCCACCAGCt                       <  1:326124/151‑1 (MQ=255)
                                                                                                                         cGTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCtt                      <  2:643869/151‑1 (MQ=255)
                                                                                                                          gTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCttt                     >  1:555672/1‑151 (MQ=255)
                                                                                                                          gTCGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCttt                     >  2:1471068/1‑151 (MQ=255)
                                                                                                                            cGAGGCGAAACGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTg                   <  2:1358874/151‑1 (MQ=255)
                                                                                                                                      cGTCTGCTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTGCTggagcgg          >  2:157849/1‑150 (MQ=255)
                                                                                                                                            cTCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTGCTGGAGCGGAGCCat    >  2:982207/1‑150 (MQ=255)
                                                                                                                                             tCCGTTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTGCTGGAGCGGAGCCatat  >  1:208256/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGGCAGAAGAAATTTAAAGTTAAAAAATAACCTTATTGTTCGCCTACAAAGCTGACAAAGCGCGTTCCTTTATAGCTCAGCGTACCTTTATCGCCCACTGTCAGGGCGTGGTACTGCTGGGCGTCGAGGCGAAACGTCTGCTCCATTCCTCCGCTTTGCGGTTTGAAGCTTGCCTCATAGCGTATACTGGTGCCTGCCGGAGTCACTTCCTGCTGGCGCGAACGGCGATCGTTAATCGGTTTTTCCCGCTTGTTGCTCACCACCACCAGCTTTTGCTGGAGCGGAGCCATAT  >  NC_000913/3605219‑3605510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: