Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,691,676 T→C Y311C (TAC→TGC)  bcsB ← regulator of cellulose synthase, cyclic di‑GMP binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,691,6760TC99.0% 396.6 / NA 104Y311C (TAC→TGC) bcsBregulator of cellulose synthase, cyclic di‑GMP binding
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (47/56);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CGTACGGCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGTAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTCTGC  >  NC_000913/3691533‑3691822
                                                                                                                                               |                                                                                                                                                  
cGTACGGCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGAtt                                                                                                                                             <  2:560606/151‑1 (MQ=255)
cGTACGGCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGAt                                                                                                                                              <  1:168610/150‑1 (MQ=255)
      gCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCgg                                                                                                                                       <  2:1282756/151‑1 (MQ=255)
      gCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCgg                                                                                                                                       <  1:914521/151‑1 (MQ=255)
          aCGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATgg                                                                                                                                   <  2:1347148/151‑1 (MQ=255)
             cgGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGtt                                                                                                                                 >  1:168666/1‑150 (MQ=255)
              ggTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTaa                                                                                                                               <  2:588603/151‑1 (MQ=255)
                 agcagcGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAatc                                                                                                                             >  2:801150/1‑150 (MQ=255)
                       ggTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCa                                                                                                                       >  2:299396/1‑150 (MQ=255)
                       ggTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCa                                                                                                                       >  1:1035997/1‑150 (MQ=255)
                        gTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAAt                                                                                                                     >  1:644851/1‑151 (MQ=255)
                         tttCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATc                                                                                                                    >  2:1143118/1‑151 (MQ=255)
                           tCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCa                                                                                                                   <  1:229931/150‑1 (MQ=255)
                           tCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCAc                                                                                                                  >  1:1122111/1‑151 (MQ=255)
                            cACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCAc                                                                                                                  <  2:887674/150‑1 (MQ=255)
                              cTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCgg                                                                                                               >  1:248043/1‑151 (MQ=255)
                                tcattcaCTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCgg                                                                                                               <  2:52549/149‑1 (MQ=255)
                                 cattcaCTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCgggg                                                                                                             <  1:1292258/150‑1 (MQ=255)
                                  attcaCTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCt                                                                                                           <  2:1441219/151‑1 (MQ=255)
                                  attcaCTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCt                                                                                                           <  1:801229/151‑1 (MQ=255)
                                     caCTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCtttt                                                                                                        <  2:1360890/151‑1 (MQ=255)
                                        tACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTAcc                                                                                                     >  2:403808/1‑151 (MQ=255)
                                           cACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTAccgcc                                                                                                  <  1:486718/151‑1 (MQ=255)
                                           cACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTAccgcc                                                                                                  >  1:633288/1‑151 (MQ=255)
                                              gCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCgg                                                                                                >  2:58147/1‑150 (MQ=255)
                                              gCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCgg                                                                                                >  2:975272/1‑150 (MQ=255)
                                               cTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGga                                                                                               <  2:977562/150‑1 (MQ=255)
                                                tttCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatg                                                                                             >  2:601972/1‑151 (MQ=255)
                                                 ttCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatg                                                                                             >  2:377531/1‑150 (MQ=255)
                                                 ttCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatg                                                                                             <  2:436288/150‑1 (MQ=255)
                                                 ttCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgat                                                                                              <  1:1046041/149‑1 (MQ=255)
                                                  tCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatga                                                                                            <  1:1061911/150‑1 (MQ=255)
                                                   caccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatgat                                                                                           <  2:1364034/150‑1 (MQ=255)
                                                   caccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGgatgat                                                                                           <  1:98244/150‑1 (MQ=255)
                                                     ccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATcgc                                                                                        <  2:835442/151‑1 (MQ=255)
                                                     ccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATcgc                                                                                        >  2:1220682/1‑151 (MQ=255)
                                                     ccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATcgc                                                                                        <  1:810995/151‑1 (MQ=255)
                                                     ccacGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATcgc                                                                                        <  2:1303801/151‑1 (MQ=255)
                                                         ggAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCgcgc                                                                                      >  1:619413/1‑149 (MQ=255)
                                                           aaCAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAgg                                                                                   >  1:878777/1‑150 (MQ=255)
                                                               gAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTc                                                                              >  2:423621/1‑151 (MQ=255)
                                                                aaTGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTcc                                                                             >  1:526555/1‑151 (MQ=255)
                                                                   gTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGc                                                                          <  2:587124/151‑1 (MQ=255)
                                                                   gTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGc                                                                          >  1:312878/1‑151 (MQ=255)
                                                                         cTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTg                                                                    >  1:373359/1‑151 (MQ=255)
                                                                           gAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTg                                                                    <  1:284576/149‑1 (MQ=255)
                                                                            aGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCg                                                                 >  1:1291357/1‑151 (MQ=255)
                                                                            aGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCg                                                                 >  2:1368659/1‑151 (MQ=255)
                                                                               gATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCgttg                                                              >  1:669072/1‑151 (MQ=255)
                                                                                 tACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCgttgg                                                             >  2:186722/1‑150 (MQ=255)
                                                                                        cGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCaa                                                       >  1:528205/1‑149 (MQ=255)
                                                                                        cGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCaa                                                       >  2:782820/1‑149 (MQ=255)
                                                                                         gCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCaa                                                       >  2:1242497/1‑148 (MQ=255)
                                                                                         gCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGa                                                    <  1:412120/151‑1 (MQ=255)
                                                                                          ctgcctgcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGAc                                                   >  2:725161/1‑151 (MQ=255)
                                                                                            gcctgcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACa                                                  >  1:644775/1‑150 (MQ=255)
                                                                                              ctgcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATg                                               >  1:10423/1‑151 (MQ=255)
                                                                                               tgcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATgg                                              >  1:1209670/1‑151 (MQ=255)
                                                                                                gcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATg                                               <  2:14331/149‑1 (MQ=255)
                                                                                                gcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGc                                             <  2:644778/151‑1 (MQ=255)
                                                                                                gcAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGc                                             >  1:432057/1‑151 (MQ=255)
                                                                                                 cAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGc                                             >  1:758203/1‑150 (MQ=255)
                                                                                                 cAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGc                                             >  2:1364743/1‑150 (MQ=255)
                                                                                                 cAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCa                                            <  1:58156/151‑1 (MQ=255)
                                                                                                 cAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCa                                            <  1:1416865/151‑1 (MQ=255)
                                                                                                  aaCAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCa                                            <  2:426320/150‑1 (MQ=255)
                                                                                                  aaCAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCAt                                           >  1:181804/1‑151 (MQ=255)
                                                                                                  aaCAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCAt                                           >  1:1381391/1‑151 (MQ=255)
                                                                                                    cAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTg                                         >  1:36430/1‑151 (MQ=255)
                                                                                                     aGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGc                                        <  1:299429/151‑1 (MQ=255)
                                                                                                      ggTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCg                                       <  2:1335441/151‑1 (MQ=255)
                                                                                                       gTCTTTGTCGTCACGACCAAACGCCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGa                                      <  1:100977/151‑1 (MQ=255)
                                                                                                          tttGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGa                                      <  2:1291252/148‑1 (MQ=255)
                                                                                                           ttGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGAt                                     <  2:852966/148‑1 (MQ=255)
                                                                                                           ttGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGAt                                     <  1:588141/148‑1 (MQ=255)
                                                                                                           ttGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATcc                                   <  2:168644/150‑1 (MQ=255)
                                                                                                            tGTCGTCACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCg                                  <  2:413591/150‑1 (MQ=255)
                                                                                                              tcgtcACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGc                                >  2:313047/1‑150 (MQ=255)
                                                                                                                  cACGACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGtt                            <  2:703172/150‑1 (MQ=255)
                                                                                                                   tcGACCAAACACCACCAGCAGTTTGACGTAAGGCTTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGGAGTCCGGCCGTTTGTCGTTGGGTGCAAAGACAATGGCATTGAGATCTGGCAGTTgg                          >  2:59422/2‑151 (MQ=255)
                                                                                                                     ggccAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCCGCAGTTGGtt                        <  2:1192102/149‑1 (MQ=255)
                                                                                                                     gACCAAACGCCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGAACGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGt                         <  1:1337702/150‑1 (MQ=255)
                                                                                                                     gACCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGtt                        >  2:489006/1‑151 (MQ=255)
                                                                                                                      aCCAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTta                       <  2:868794/151‑1 (MQ=255)
                                                                                                                       ccAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTtat                      <  1:403099/151‑1 (MQ=255)
                                                                                                                        cAAACACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTtata                     <  2:1039505/151‑1 (MQ=255)
                                                                                                                         aaaCACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATag                    <  2:1139251/151‑1 (MQ=255)
                                                                                                                          aaCACCGCCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATaga                   <  2:291128/151‑1 (MQ=255)
                                                                                                                          aaCACCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTtat                      >  1:97432/1‑148 (MQ=255)
                                                                                                                           acacCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATagag                  <  2:1197376/151‑1 (MQ=255)
                                                                                                                           acacCACCAGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATag                    >  1:1011798/1‑149 (MQ=255)
                                                                                                                              ccaccaGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTAc               >  1:983324/1‑151 (MQ=255)
                                                                                                                              ccaccaGCAGTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTAc               >  1:898612/1‑151 (MQ=255)
                                                                                                                                   agcagTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGa          <  1:1189080/151‑1 (MQ=255)
                                                                                                                                       gTTTGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTAACGGGAAGtt      <  2:1122013/151‑1 (MQ=255)
                                                                                                                                        tttGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGtt      <  2:782906/150‑1 (MQ=255)
                                                                                                                                        tttGACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTc     >  1:851468/1‑151 (MQ=255)
                                                                                                                                           gACGCAAGGATTCTGCGTATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTCTGc  <  2:1035897/151‑1 (MQ=255)
                                                                                                                                           gACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTCTg   <  2:474824/150‑1 (MQ=255)
                                                                                                                                           gACGCAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTCTg   <  1:1143214/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGTACGGCTTACGCGGTAGCAGCGGTTTCACTTCATTCACTACCACGCTTTCACCACGGAACAGAATGTTACCCTGAGCGATACCTTTCGCTGCCTGCAACAGGTCTTTGTCGTCACGACCAAACACCACCAGCAGTTTGACGTAAGGATTCTGCGGATGGTTAATCATCTCAATCACCGGGGCTTTTACCGCCGGATGATCGCGCAGGAAGTCCGGCCGTTTGTCGTTGGTTGCAAAGACAATGGCATTGCGATCCGGCAGTTGGTTATAGAGTACCGGGAAGTTCTGC  >  NC_000913/3691533‑3691822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: