Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,140,495	T→C	D576G (GAT→GGT)	frwA ←	putative PTS enzyme: Hpr, enzyme I and II components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,140,495	0	T	C	100.0%	271.5 / NA	76	D576G (GAT→GGT)	frwA	putative PTS enzyme: Hpr, enzyme I and II components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/44);  total (32/44)

CGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG  >  NC_000913/4140347‑4140641                                                                                                                                                     |                                                                                                                                                   cGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCCCGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGAccc                                                                                                                                                  >  2:1357245/1‑151 (MQ=255)  ggCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCa                                                                                                                                                 <  2:242606/151‑1 (MQ=255)    cagcaCGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCAcc                                                                                                                                               <  2:616366/151‑1 (MQ=255)      gcaCGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGc                                                                                                                                             >  1:916763/1‑151 (MQ=255)      gcaCGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGc                                                                                                                                             >  2:401258/1‑151 (MQ=255)            aaCCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCcagca                                                                                                                                        <  1:883013/150‑1 (MQ=255)                   cGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTg                                                                                                                                 <  1:480680/150‑1 (MQ=255)                     cTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGt                                                                                                                              >  1:337722/1‑151 (MQ=255)                      tCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTc                                                                                                                             >  1:900820/1‑151 (MQ=255)                       cccAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGt                                                                                                                              <  1:162038/149‑1 (MQ=255)                           gCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTc                                                                                                                        <  2:41211/151‑1 (MQ=255)                            cTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTc                                                                                                                        <  2:833727/150‑1 (MQ=255)                             tCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCAt                                                                                                                      >  2:1023948/1‑151 (MQ=255)                                ccGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATtac                                                                                                                   >  2:1008691/1‑151 (MQ=255)                                ccGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTCAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATta                                                                                                                    <  2:530126/150‑1 (MQ=255)                                  gCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATtact                                                                                                                  >  2:314825/1‑150 (MQ=255)                                     caGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCa                                                                                                              <  1:335905/151‑1 (MQ=255)                                       gACCAATCCATTTGCCCTGGCGAGGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTAGCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATtacta                                                                                                                 <  2:153692/146‑1 (MQ=255)                                        aCCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAAt                                                                                                            >  1:412222/1‑150 (MQ=255)                                           aaTCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACt                                                                                                         >  1:115355/1‑150 (MQ=255)                                           aaTCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTa                                                                                                        <  2:861991/151‑1 (MQ=255)                                              ccgtttGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTaa                                                                                                       >  2:2380/4‑149 (MQ=255)                                                ctttGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGa                                                                                                    >  2:384927/2‑150 (MQ=255)                                                aTTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGaa                                                                                                   <  2:866619/151‑1 (MQ=255)                                                   tGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatc                                                                                                >  2:147391/1‑151 (MQ=255)                                                    gCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatc                                                                                                <  2:37389/150‑1 (MQ=255)                                                     cccTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatca                                                                                               <  2:572479/150‑1 (MQ=255)                                                      ccTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatcaat                                                                                             >  2:1467773/1‑151 (MQ=255)                                                        tGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAAtct                                                                                           >  2:761239/1‑151 (MQ=255)                                                         ggCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatcaatc                                                                                            <  1:689508/149‑1 (MQ=255)                                                         ggCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAaatcaatc                                                                                            <  1:219475/149‑1 (MQ=255)                                                         ggCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAAtct                                                                                           <  1:1295969/150‑1 (MQ=255)                                                          gCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATctct                                                                                         <  2:452272/151‑1 (MQ=255)                                                               gcgccGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCgc                                                                                     <  2:127067/147‑1 (MQ=255)                                                               gCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCgca                                                                                    <  2:670608/151‑1 (MQ=255)                                                               gCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCgca                                                                                    <  2:79649/151‑1 (MQ=255)                                                                  ccGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCgcagca                                                                                 <  2:646963/151‑1 (MQ=255)                                                                    gCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGCCCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCAtt                                                                               <  2:803367/151‑1 (MQ=255)                                                                    gCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCAtt                                                                               >  1:166688/1‑151 (MQ=255)                                                                      ttGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTg                                                                              >  2:68657/1‑150 (MQ=255)                                                                       tGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTg                                                                              <  2:1286218/149‑1 (MQ=255)                                                                           cGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCgat                                                                        <  1:500719/151‑1 (MQ=255)                                                                             gCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCgatga                                                                      <  1:91139/151‑1 (MQ=255)                                                                              cGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCgatgat                                                                     <  1:760755/151‑1 (MQ=255)                                                                               gTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCgatgat                                                                     >  2:891199/1‑150 (MQ=255)                                                                                 aaTCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGAtgatga                                                                   >  2:494364/1‑150 (MQ=255)                                                                                   tCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAAc                                                                 >  2:257806/1‑150 (MQ=255)                                                                                   tCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAAc                                                                 >  1:1053239/1‑150 (MQ=255)                                                                                     gAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACAt                                                               >  2:281013/1‑150 (MQ=255)                                                                                         gcCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCg                                                          >  2:770870/1‑151 (MQ=255)                                                                                           ccGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGAc                                                        <  1:326667/151‑1 (MQ=255)                                                                                           ccGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGAc                                                        >  2:918235/1‑151 (MQ=255)                                                                                            cGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCGCCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCg                                                          <  2:916676/148‑1 (MQ=255)                                                                                            cGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACg                                                       <  2:762854/151‑1 (MQ=255)                                                                                                  aaTGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACtt                                                 >  2:1375247/1‑151 (MQ=255)                                                                                                  aaTGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACtt                                                 <  1:286210/151‑1 (MQ=255)                                                                                                  aaTGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACtt                                                 >  1:287389/1‑151 (MQ=255)                                                                                                      ccGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTcc                                               >  1:746676/1‑149 (MQ=255)                                                                                                          aTTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCatg                                         <  1:596838/151‑1 (MQ=255)                                                                                                               ggCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGcc                                    >  1:810279/1‑151 (MQ=255)                                                                                                                   gttgtAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGc                                >  1:1075902/1‑151 (MQ=255)                                                                                                                     tgtAGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCt                               <  2:184829/150‑1 (MQ=255)                                                                                                                        aGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGa                            <  1:321511/150‑1 (MQ=255)                                                                                                                        aGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAt                           <  2:1250603/151‑1 (MQ=255)                                                                                                                        aGTGACGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAt                           >  2:898269/1‑151 (MQ=255)                                                                                                                            aCGAGTAACCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAtttt                        <  1:1262987/150‑1 (MQ=255)                                                                                                                                   aCCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtcatca                 >  2:1381946/1‑150 (MQ=255)                                                                                                                                   aCCTTAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaa                <  1:1311540/151‑1 (MQ=255)                                                                                                                                    ccTTAGCGTTAGCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAACCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaaa               <  2:466959/151‑1 (MQ=255)                                                                                                                                       tAGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACg             <  2:70873/150‑1 (MQ=255)                                                                                                                                        aGCGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGCTTTTCTCATCAAACgg            >  2:1013306/1‑150 (MQ=255)                                                                                                                                          cGTTATCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAt         <  1:588969/151‑1 (MQ=255)                                                                                                                                              aTCGCGACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtgtg      <  1:401313/150‑1 (MQ=255)                                                                                                                                                cgcgACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTc   <  1:426260/151‑1 (MQ=255)                                                                                                                                                 gcgACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg  <  2:412167/151‑1 (MQ=255)                                                                                                                                                 gcgACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg  >  2:435481/1‑151 (MQ=255)                                                                                                                                                  cgACCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg  <  1:1325534/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGTAATCGAGCGCCCGCAAGAATGCCGGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG  >  NC_000913/4140347‑4140641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑