Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,185,098	A→G	Y1285C (TAC→TGC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,185,098	0	A	G	100.0%	378.4 / NA	97	Y1285C (TAC→TGC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (57/40);  total (57/40)

AACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTG  >  NC_000913/4184950‑4185243                                                                                                                                                     |                                                                                                                                                  aaCCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATgca                                                                                                                                                 <  2:753905/151‑1 (MQ=255) aaCCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATgca                                                                                                                                                 <  2:248496/151‑1 (MQ=255)    caccTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCAccc                                                                                                                                              >  2:485503/1‑151 (MQ=255)       cTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGc                                                                                                                                           >  2:476772/1‑151 (MQ=255)         ggTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCa                                                                                                                                          <  1:809080/150‑1 (MQ=255)         ggTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAg                                                                                                                                         <  1:192255/151‑1 (MQ=255)                 acAAGATGCACGCGCGTTCCTCCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCt                                                                                                                                 <  1:1199064/151‑1 (MQ=255)                  cAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTc                                                                                                                                <  1:341191/151‑1 (MQ=255)                   aaGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCa                                                                                                                               <  1:552619/151‑1 (MQ=255)                    aGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCa                                                                                                                               <  1:1063094/150‑1 (MQ=255)                        gCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGt                                                                                                                           <  1:834529/150‑1 (MQ=255)                          aCGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTa                                                                                                                         >  1:1074774/1‑150 (MQ=255)                           cgcgcgTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAg                                                                                                                       <  2:1075314/151‑1 (MQ=255)                            gcgcgTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGt                                                                                                                      >  1:549983/1‑151 (MQ=255)                             cgcgTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTc                                                                                                                     >  1:1140983/1‑151 (MQ=255)                               cgTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCtg                                                                                                                   >  1:1130971/1‑151 (MQ=255)                               cgTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCtg                                                                                                                   <  1:666767/151‑1 (MQ=255)                                  tCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCtgatg                                                                                                                >  2:780097/1‑151 (MQ=255)                                    cACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGAc                                                                                                              >  1:893881/1‑151 (MQ=255)                                    cACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGAc                                                                                                              <  1:1209863/151‑1 (MQ=255)                                      ccGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGt                                                                                                            >  1:104745/1‑151 (MQ=255)                                         gTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGa                                                                                                          >  1:1263324/1‑150 (MQ=255)                                          ttCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAAc                                                                                                        >  1:687664/1‑151 (MQ=255)                                          ttCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAAc                                                                                                        >  2:1030812/1‑151 (MQ=255)                                            cTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACgg                                                                                                      >  1:1300291/1‑151 (MQ=255)                                            cTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACgg                                                                                                      >  1:122706/1‑151 (MQ=255)                                              tACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACg                                                                                                       <  1:895575/148‑1 (MQ=255)                                              tACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTc                                                                                                    >  2:985507/1‑151 (MQ=255)                                               aCAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTc                                                                                                    <  1:1343963/150‑1 (MQ=255)                                                cAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGt                                                                                                  >  1:1117149/1‑151 (MQ=255)                                                cAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGt                                                                                                  <  2:1472652/151‑1 (MQ=255)                                                    cTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTAcc                                                                                               >  2:459545/1‑150 (MQ=255)                                                       gTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGa                                                                                           <  2:552689/151‑1 (MQ=255)                                                         tACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATg                                                                                         >  2:744267/1‑151 (MQ=255)                                                              agcagcCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGtata                                                                                     >  1:1193201/1‑150 (MQ=255)                                                                  gcCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAAc                                                                                >  2:1244131/1‑151 (MQ=255)                                                                  gcCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAAc                                                                                >  1:1417221/1‑151 (MQ=255)                                                                    cGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACAt                                                                              >  1:975368/1‑151 (MQ=255)                                                                    cGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACAt                                                                              >  1:482727/1‑151 (MQ=255)                                                                       tGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGt                                                                           >  1:819423/1‑151 (MQ=255)                                                                        gggTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCg                                                                            <  2:1122093/149‑1 (MQ=255)                                                                        gggTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGt                                                                           <  2:1386103/150‑1 (MQ=255)                                                                        gggTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTg                                                                          >  1:191099/1‑151 (MQ=255)                                                                          gtggtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGa                                                                        <  2:235924/151‑1 (MQ=255)                                                                          gtggtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGa                                                                        <  1:619161/151‑1 (MQ=255)                                                                           tggtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGAc                                                                       >  2:273849/1‑151 (MQ=255)                                                                            ggtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACg                                                                      <  1:1138787/151‑1 (MQ=255)                                                                             gtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACgg                                                                     <  1:114128/151‑1 (MQ=255)                                                                             gtAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACgg                                                                     >  2:770423/1‑151 (MQ=255)                                                                                   cacaGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc                                                                >  2:1161990/1‑150 (MQ=255)                                                                                    acaGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAAcc                                                                >  2:1458043/1‑149 (MQ=255)                                                                                    acaGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCAt                                                              >  1:889628/1‑151 (MQ=255)                                                                                    acaGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGAGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCAt                                                              >  1:412424/1‑151 (MQ=255)                                                                                      aGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCa                                                            <  1:1396681/151‑1 (MQ=255)                                                                                      aGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCa                                                            <  1:1166269/151‑1 (MQ=255)                                                                                          cGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATg                                                        <  2:242830/151‑1 (MQ=255)                                                                                           ggtggtCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATgg                                                       <  1:655289/151‑1 (MQ=255)                                                                                              ggtCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGc                                                    >  1:1471926/1‑151 (MQ=255)                                                                                                tCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCg                                                  >  1:1012359/1‑151 (MQ=255)                                                                                                 cAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCgg                                                 <  1:476827/151‑1 (MQ=255)                                                                                                  aGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCggg                                                >  2:745487/1‑151 (MQ=255)                                                                                                   gCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGc                                               <  1:1449377/151‑1 (MQ=255)                                                                                                    cGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGc                                               >  2:801483/1‑150 (MQ=255)                                                                                                    cGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGc                                               >  2:330973/1‑150 (MQ=255)                                                                                                     gTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCAt                                             <  2:889540/151‑1 (MQ=255)                                                                                                     gTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCAt                                             >  1:1162738/1‑151 (MQ=255)                                                                                                     gTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCAt                                             >  2:1359707/1‑151 (MQ=255)                                                                                                        tCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGcc                                          >  2:715351/1‑151 (MQ=255)                                                                                                        tCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGc                                           <  2:143498/150‑1 (MQ=255)                                                                                                         cGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCa                                         <  1:342197/151‑1 (MQ=255)                                                                                                          ggggAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCATACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAg                                        <  2:1261809/151‑1 (MQ=255)                                                                                                             gagaTGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAAt                                     >  2:1191193/1‑151 (MQ=255)                                                                                                             gagaTGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAAt                                     >  1:14204/1‑151 (MQ=255)                                                                                                              agaTGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATc                                    >  1:693078/1‑151 (MQ=255)                                                                                                               gaTGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATcc                                   <  2:752204/151‑1 (MQ=255)                                                                                                                    aaGTGTGGGCGCTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCaa                              <  2:975273/151‑1 (MQ=255)                                                                                                                            gcgcTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgt                      >  2:955945/1‑151 (MQ=255)                                                                                                                            gcgcTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgt                      <  2:1467832/151‑1 (MQ=255)                                                                                                                            gcgcTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgt                      <  1:76459/151‑1 (MQ=255)                                                                                                                            gcgcTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgt                      <  1:1326006/151‑1 (MQ=255)                                                                                                                              gcTGGAAGCATACGGCGCAGCATGCATCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgttg                    >  2:779897/1‑151 (MQ=255)                                                                                                                              gcTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgttg                    >  1:653819/1‑151 (MQ=255)                                                                                                                               cTGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgttg                    >  1:908265/1‑150 (MQ=255)                                                                                                                                tGGAAGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTAttgttg                    >  1:1268639/1‑149 (MQ=255)                                                                                                                                   aaGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAagg               <  1:357963/151‑2 (MQ=255)                                                                                                                                   aaGCATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAaga               <  2:687589/151‑1 (MQ=255)                                                                                                                                      cATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAGCCATCAGATGGAGCCGGGCATGCCAGAATCCTTAAACGTATTGTTGAAAGAGAt            <  2:1472425/151‑1 (MQ=255)                                                                                                                                      cATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAag                >  1:779389/1‑147 (MQ=255)                                                                                                                                      cATACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAag                >  1:938380/1‑147 (MQ=255)                                                                                                                                       aTACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt           <  1:96600/151‑1 (MQ=255)                                                                                                                                       aTACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt           >  1:735469/1‑151 (MQ=255)                                                                                                                                       aTACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt           >  1:690084/1‑151 (MQ=255)                                                                                                                                       aTACGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtt           >  1:595193/1‑151 (MQ=255)                                                                                                                                         aCGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcg         >  2:609169/1‑151 (MQ=255)                                                                                                                                         aCGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcg         >  1:631048/1‑151 (MQ=255)                                                                                                                                         aCGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcg         >  1:1339590/1‑151 (MQ=255)                                                                                                                                          cGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgt        <  2:412126/151‑1 (MQ=255)                                                                                                                                          cGGCGCAGCATGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttcgt        >  1:1024275/1‑151 (MQ=255)                                                                                                                                                agcaTGCACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAATCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTg  >  1:1141381/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCAGCATACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTG  >  NC_000913/4184950‑4185243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑