Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,460,207 T→C D53G (GAC→GGC)  nrdG ← anaerobic ribonucleotide reductase activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,2070TC100.0% 334.4 / NA 92D53G (GAC→GGC) nrdGanaerobic ribonucleotide reductase activating protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/41);  total (51/41)

CCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACTGAT  >  NC_000913/4460059‑4460354
                                                                                                                                                    |                                                                                                                                                   
ccAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCt                                                                                                                                                   >  2:881937/1‑151 (MQ=255)
 cAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCtt                                                                                                                                                  <  2:485235/151‑1 (MQ=255)
    aTGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCa                                                                                                                                               <  2:1200442/151‑1 (MQ=255)
      gTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATGCCCTGGCGTTTGATACGTGTGTCATTCAGATCGTTAATGATCTGGCCTTCCAt                                                                                                                                              <  1:660364/150‑1 (MQ=255)
       tCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTg                                                                                                                                            >  2:696130/1‑151 (MQ=255)
       tCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCCGGCCTTCCAtt                                                                                                                                             <  2:298996/150‑1 (MQ=255)
          ttACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCtt                                                                                                                                         >  2:380769/1‑151 (MQ=255)
          ttACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCtt                                                                                                                                         >  2:882456/1‑151 (MQ=255)
           tACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCttt                                                                                                                                        >  1:1113655/1‑151 (MQ=255)
            aCCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCttt                                                                                                                                        <  1:407047/150‑1 (MQ=255)
             cccGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTgg                                                                                                                                      <  2:406421/151‑1 (MQ=255)
               cGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTa                                                                                                                                    <  1:983222/151‑1 (MQ=255)
                ggACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTa                                                                                                                                    >  2:476546/1‑150 (MQ=255)
                ggACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTa                                                                                                                                    >  2:28647/1‑150 (MQ=255)
                  acacTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAAt                                                                                                                                 <  1:791002/151‑1 (MQ=255)
                   cacTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATg                                                                                                                                <  1:1204490/151‑1 (MQ=255)
                    acTCGGCGCGGATGCGTTGTACCAGTTTCAGAATGTCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATgg                                                                                                                               <  2:881164/151‑1 (MQ=255)
                    acTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATgg                                                                                                                               <  1:473222/151‑1 (MQ=255)
                     cTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGc                                                                                                                              >  1:1249932/1‑151 (MQ=255)
                       cGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCt                                                                                                                             <  1:109478/150‑1 (MQ=255)
                       cGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTg                                                                                                                            >  2:970897/1‑151 (MQ=255)
                           gcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCg                                                                                                                        <  2:1218095/151‑1 (MQ=255)
                            cgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCgg                                                                                                                       <  1:435074/151‑1 (MQ=255)
                               aTGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAt                                                                                                                    <  1:1419347/151‑1 (MQ=255)
                               aTGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAt                                                                                                                    <  2:1147644/151‑1 (MQ=255)
                                tGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAtt                                                                                                                   >  2:594201/1‑151 (MQ=255)
                                 gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAttt                                                                                                                  <  2:1186112/151‑1 (MQ=255)
                                 gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAtt                                                                                                                   <  1:427790/150‑1 (MQ=255)
                                  cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAAttt                                                                                                                  >  2:865488/1‑150 (MQ=255)
                                  cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTAc                                                                                                                <  1:149089/151‑1 (MQ=255)
                                      gTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCg                                                                                                             <  2:321161/151‑1 (MQ=255)
                                      gTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGAACGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCg                                                                                                             >  2:447207/1‑151 (MQ=255)
                                        aCCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGcc                                                                                                           >  2:186256/1‑151 (MQ=255)
                                         ccAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGcc                                                                                                           >  2:550936/1‑150 (MQ=255)
                                            gTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGTGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCAtg                                                                                                        >  1:1324559/1‑150 (MQ=255)
                                              ttCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGc                                                                                                     >  2:41311/1‑151 (MQ=255)
                                                 aGAATATCCGGCCCGTTTTGCGGATGCAGCGGAGCGCCGCCGGGGAGGTAAATACCCTGGCGTTGGATACGATTGTCATTCAGATCTTAAAGAATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGAAATTTCCCCGCCAtgtg                                                                                                      <  2:847940/147‑1 (MQ=255)
                                                   aaTATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATCCCCTGGCGTTTGATACGGGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTg                                                                                                <  2:441105/151‑1 (MQ=255)
                                                   aaTATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCtttt                                                                                                 <  1:1197007/150‑1 (MQ=255)
                                                    atatCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGt                                                                                               <  2:206970/151‑1 (MQ=255)
                                                       tCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGAAATTTACCCGCCATGTGCTTTTGtt                                                                                              <  2:1209245/149‑1 (MQ=255)
                                                        ccGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTta                                                                                             <  1:1261678/149‑1 (MQ=255)
                                                               gTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCg                                                                                    >  1:1148027/1‑151 (MQ=255)
                                                                  ttGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCggg                                                                                  <  1:1036265/150‑1 (MQ=255)
                                                                        aTGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTc                                                                            >  1:348365/1‑150 (MQ=255)
                                                                         tGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTCCCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCa                                                                           <  1:476601/150‑1 (MQ=255)
                                                                            aGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTTTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCa                                                                           <  1:660348/147‑1 (MQ=255)
                                                                                gATCGCCGCCGGAGAGGGAAATTCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAaca                                                                     <  1:285975/149‑1 (MQ=255)
                                                                                 aTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAacacac                                                                  >  2:977422/1‑151 (MQ=255)
                                                                                      cgccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                                                              >  2:235579/1‑150 (MQ=255)
                                                                                      cgccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                                                              >  1:1052111/1‑150 (MQ=255)
                                                                                      cgccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                                                              >  1:478958/1‑150 (MQ=255)
                                                                                      cgccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCg                                                               >  2:386944/1‑149 (MQ=255)
                                                                                       gccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                                                              >  1:400734/1‑149 (MQ=255)
                                                                                        ccgGAGAGGGAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGgaga                                                           <  2:1439669/151‑1 (MQ=255)
                                                                                        ccgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                                                              >  1:862499/1‑148 (MQ=255)
                                                                                         cgGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGAc                                                          >  2:432283/1‑151 (MQ=255)
                                                                                                  aaaTACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGt                                                 >  1:623663/1‑151 (MQ=255)
                                                                                                   aaTACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTg                                                >  2:1255605/1‑151 (MQ=255)
                                                                                                       cccTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTCCCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCa                                              <  2:444133/149‑1 (MQ=255)
                                                                                                       cccTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAAc                                            >  2:1334839/1‑151 (MQ=255)
                                                                                                       cccTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAAc                                            >  2:1082294/1‑151 (MQ=255)
                                                                                                        ccTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACg                                           >  2:352987/1‑151 (MQ=255)
                                                                                                         cTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACg                                           >  2:180651/1‑150 (MQ=255)
                                                                                                         cTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACg                                           >  2:1293890/1‑150 (MQ=255)
                                                                                                           ggCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGt                                        <  1:506611/151‑1 (MQ=255)
                                                                                                             cGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGc                                      >  2:221123/1‑151 (MQ=255)
                                                                                                              gTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGcc                                     >  1:1141224/1‑151 (MQ=255)
                                                                                                                 tGATACGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCggg                                  >  1:598218/1‑151 (MQ=255)
                                                                                                                     aCGAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCg                              >  2:1345045/1‑151 (MQ=255)
                                                                                                                       gAGTGTCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGtt                            <  1:696209/151‑1 (MQ=255)
                                                                                                                          tgtCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGa                          <  1:519513/150‑1 (MQ=255)
                                                                                                                          tgtCATTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGAc                         >  1:1314874/1‑151 (MQ=255)
                                                                                                                              aTTCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATg                     >  1:1386288/1‑151 (MQ=255)
                                                                                                                               ttCAGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGt                    >  1:813979/1‑151 (MQ=255)
                                                                                                                                  aGATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGa                 <  1:380819/151‑1 (MQ=255)
                                                                                                                                   gATCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGa                 >  2:147613/1‑150 (MQ=255)
                                                                                                                                     tCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGAc                >  2:187230/1‑149 (MQ=255)
                                                                                                                                     tCGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACa               <  2:1147931/150‑1 (MQ=255)
                                                                                                                                      cGTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAgg             >  1:1201728/1‑151 (MQ=255)
                                                                                                                                       gTTAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAgg             >  2:482233/1‑150 (MQ=255)
                                                                                                                                         tAATGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGAt           >  1:120497/1‑150 (MQ=255)
                                                                                                                                          aaTGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATa          <  1:778987/150‑1 (MQ=255)
                                                                                                                                          aaTGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAg         <  1:501209/151‑1 (MQ=255)
                                                                                                                                            tGATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGt        >  2:410548/1‑150 (MQ=255)
                                                                                                                                             gATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTa       <  1:28650/150‑1 (MQ=255)
                                                                                                                                             gATCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTAc      >  1:539563/1‑151 (MQ=255)
                                                                                                                                              aTCTGGCCTCCCTTTGCTTTGGTAAATGGCTGACCGAAATTAACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGt        <  2:1113556/148‑1 (MQ=255)
                                                                                                                                               tCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACtg    >  2:567483/1‑151 (MQ=255)
                                                                                                                                               tCTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACtg    >  1:266108/1‑151 (MQ=255)
                                                                                                                                                cTGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACtg    >  1:1282399/1‑150 (MQ=255)
                                                                                                                                                 tGGCCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACtgat  >  1:789667/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAACGAGTGCCGGGGCCGTTGACGATGTCGACAGGATAGTACTGAT  >  NC_000913/4460059‑4460354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: