Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,516,435 G→A S81S (AGC→AGT fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,516,4350GA99.1% 432.0 / ‑4.5 111S81S (AGC→AGTfecATonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (59/51);  total (60/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 111 total aligned reads displayed.

ATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCA  >  NC_000913/4516287‑4516582
                                                                                                                                                    |                                                                                                                                                   
  aCGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCActct                                                                                                                                                 >  2:649216/1‑151 (MQ=255)
  aCGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCActct                                                                                                                                                 <  1:772630/151‑1 (MQ=255)
       gtTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGAcg                                                                                                                                            >  2:363556/1‑151 (MQ=255)
         tttCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGAcgtc                                                                                                                                          >  2:1420557/1‑151 (MQ=255)
           tCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGAcgtcgt                                                                                                                                        >  2:1402504/1‑151 (MQ=255)
           tCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGAcgtcg                                                                                                                                         >  1:843919/1‑150 (MQ=255)
            cACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGAcgtcgt                                                                                                                                        <  1:1456061/150‑1 (MQ=255)
              cgcgCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTaa                                                                                                                                      <  2:1096862/150‑1 (MQ=255)
              cgcgCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAAt                                                                                                                                     >  1:1323841/1‑151 (MQ=255)
               gcgcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATc                                                                                                                                    >  2:255632/1‑151 (MQ=255)
               gcgcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATc                                                                                                                                    <  2:1150592/151‑1 (MQ=255)
               gcgcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATc                                                                                                                                    >  1:1252659/1‑151 (MQ=255)
                  cATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGcc                                                                                                                                 >  1:944885/1‑151 (MQ=255)
                    tCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGt                                                                                                                               >  2:801624/1‑151 (MQ=255)
                     cACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGAAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGCCGTAATCGCCGTg                                                                                                                              >  2:1270806/1‑151 (MQ=255)
                      aCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGc                                                                                                                             >  2:739789/1‑151 (MQ=255)
                       accagccagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGc                                                                                                                             >  1:1120184/2‑150 (MQ=255)
                         cagccagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAgg                                                                                                                          >  2:1127986/1‑151 (MQ=255)
                             cagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGt                                                                                                                      <  1:1084763/151‑1 (MQ=255)
                                tCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGc                                                                                                                   >  2:156482/1‑151 (MQ=255)
                                 cGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGct                                                                                                                  >  2:415763/1‑151 (MQ=255)
                                     gACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGc                                                                                                              >  2:849787/1‑151 (MQ=255)
                                       ccACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCtt                                                                                                            <  2:508688/151‑1 (MQ=255)
                                        cACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTg                                                                                                           >  1:28726/1‑151 (MQ=255)
                                          cGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGcc                                                                                                         >  1:893961/1‑151 (MQ=255)
                                          cGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGcc                                                                                                         >  1:517192/1‑151 (MQ=255)
                                            gTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCcgc                                                                                                       >  2:1186084/1‑151 (MQ=255)
                                                   cATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTc                                                                                                  >  1:1190041/1‑149 (MQ=255)
                                                     tcttctTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGGAATCGCCGTGCAGGCCGTTGCTCTGCTTGCcgcgcgcc                                                                                                  >  2:708908/1‑145 (MQ=255)
                                                      cttcttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCt                                                                                             >  2:843726/1‑151 (MQ=255)
                                                        tcttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCt                                                                                             <  2:1106209/149‑1 (MQ=255)
                                                        tcttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTgg                                                                                           >  1:476161/1‑151 (MQ=255)
                                                           tttGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGc                                                                                          <  1:774302/149‑1 (MQ=255)
                                                           tttGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCg                                                                                         <  1:438613/150‑1 (MQ=255)
                                                             tGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTc                                                                                       >  2:26981/1‑150 (MQ=255)
                                                               gTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAc                                                                                    >  1:727980/1‑151 (MQ=255)
                                                               gTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAc                                                                                    <  2:749093/151‑1 (MQ=255)
                                                                 gcgggcgcgggcTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCg                                                                                  <  2:522372/151‑1 (MQ=255)
                                                                   gggcgcgggcTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCgag                                                                                <  2:1316410/151‑1 (MQ=255)
                                                                    ggcgcgggcTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCgaga                                                                               <  1:1164123/151‑1 (MQ=255)
                                                                       gcgggcTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAggg                                                                            >  2:397731/1‑151 (MQ=255)
                                                                             tCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATc                                                                      >  2:412978/1‑151 (MQ=255)
                                                                                aGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGc                                                                   >  1:1233789/1‑151 (MQ=255)
                                                                                 gCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCt                                                                  >  2:1088489/1‑151 (MQ=255)
                                                                                   gTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgt                                                                >  1:212537/1‑151 (MQ=255)
                                                                                   gTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgt                                                                >  1:1219282/1‑151 (MQ=255)
                                                                                   gTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgt                                                                >  1:975999/1‑151 (MQ=255)
                                                                                       aGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtg                                                                 >  1:115156/1‑146 (MQ=255)
                                                                                       aGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtg                                                                 <  2:115139/146‑1 (MQ=255)
                                                                                       aGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGc                                                            <  1:26984/151‑1 (MQ=255)
                                                                                        gCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCg                                                           <  1:825339/151‑1 (MQ=255)
                                                                                         cTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCgg                                                          >  2:472699/1‑151 (MQ=255)
                                                                                         cTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCgg                                                          >  2:920622/1‑151 (MQ=255)
                                                                                         cTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCg                                                           <  1:1128083/150‑1 (MQ=255)
                                                                                          tGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                         >  2:255148/1‑151 (MQ=255)
                                                                                           gTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                         >  2:1286872/1‑150 (MQ=255)
                                                                                           gTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCa                                                        <  1:785043/151‑1 (MQ=255)
                                                                                             tATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCAt                                                       <  1:376684/150‑1 (MQ=255)
                                                                                              aTTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATa                                                      <  2:681077/150‑1 (MQ=255)
                                                                                              aTTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATAc                                                     <  1:8933/151‑1 (MQ=255)
                                                                                                ttCCCAGCGGTTTTACCTGCATTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATa                                                      <  1:1207629/148‑1 (MQ=255)
                                                                                                ttCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACt                                                    <  1:1427900/150‑1 (MQ=255)
                                                                                                ttCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTg                                                   >  1:343179/1‑151 (MQ=255)
                                                                                                  cccAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGAt                                                 <  1:782998/151‑1 (MQ=255)
                                                                                                   ccAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGAtt                                                >  2:904524/1‑151 (MQ=255)
                                                                                                      gCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                             <  2:476106/151‑1 (MQ=255)
                                                                                                      gCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                             <  1:182312/151‑1 (MQ=255)
                                                                                                       cGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                             <  1:637398/150‑1 (MQ=255)
                                                                                                        ggTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAgcg                                           <  2:152205/151‑1 (MQ=255)
                                                                                                         gctttACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAgcgc                                          <  2:7686/149‑1 (MQ=255)
                                                                                                         gTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAgcgc                                          >  2:100598/1‑151 (MQ=255)
                                                                                                           tttACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCtt                                        >  2:728580/1‑151 (MQ=255)
                                                                                                           tttACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCt                                         <  2:1108068/150‑1 (MQ=255)
                                                                                                            ttACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCtt                                        <  1:532253/150‑1 (MQ=255)
                                                                                                             tACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCttt                                       <  1:1445223/150‑1 (MQ=255)
                                                                                                             tACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCttt                                       >  1:2181/1‑150 (MQ=255)
                                                                                                               ccTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATc                                    >  1:757905/1‑151 (MQ=255)
                                                                                                               ccTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATc                                    <  1:1280998/151‑1 (MQ=255)
                                                                                                                  gCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCg                                   <  2:517134/149‑1 (MQ=255)
                                                                                                                  gCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAg                                 <  1:255173/151‑1 (MQ=255)
                                                                                                                  gCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAg                                 <  2:28723/151‑1 (MQ=255)
                                                                                                                   cAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGc                                <  1:1420664/151‑1 (MQ=255)
                                                                                                                     gTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGa                              <  1:462771/151‑1 (MQ=255)
                                                                                                                       ccGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATc                            <  1:1085212/151‑1 (MQ=255)
                                                                                                                         gCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATccc                          >  1:1408427/1‑151 (MQ=255)
                                                                                                                           tGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCgg                        >  1:971573/1‑151 (MQ=255)
                                                                                                                           tGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCgg                        >  1:1302554/1‑151 (MQ=255)
                                                                                                                            gCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCgg                        >  1:113181/1‑150 (MQ=255)
                                                                                                                            gCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGt                       >  2:1038307/1‑151 (MQ=255)
                                                                                                                              cGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGc                     <  1:747412/151‑1 (MQ=255)
                                                                                                                                  cagcagTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGat                  >  2:358151/1‑150 (MQ=255)
                                                                                                                                   agcagTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGatat                <  1:863507/151‑1 (MQ=255)
                                                                                                                                     cagTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa              <  1:880701/151‑1 (MQ=255)
                                                                                                                                      agTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa              >  2:1307680/1‑150 (MQ=255)
                                                                                                                                      agTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa              >  2:1006903/1‑150 (MQ=255)
                                                                                                                                      agTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa              >  1:956824/1‑150 (MQ=255)
                                                                                                                                       gttgttGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTaa             <  1:494928/150‑1 (MQ=255)
                                                                                                                                          gttgCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCtg         <  2:1252560/151‑1 (MQ=255)
                                                                                                                                              cAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTgca     <  1:649290/151‑1 (MQ=255)
                                                                                                                                                 gCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGGAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTgca     <  2:431274/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCA  >  NC_000913/4516287‑4516582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: