Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,552,990 C→T C30C (TGC→TGT uxuB → D‑mannonate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,552,9900CT100.0% 473.2 / NA 115C30C (TGC→TGTuxuBD‑mannonate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/65);  total (50/65)

Only 100 of 115 total aligned reads displayed.

GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTAC  >  NC_000913/4552842‑4553137
                                                                                                                                                    |                                                                                                                                                   
gcCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTgg                                                                                                                                                   >  2:1346223/1‑151 (MQ=255)
   gTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGc                                                                                                                                                <  1:299838/151‑1 (MQ=255)
    tGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCg                                                                                                                                               >  2:909845/1‑151 (MQ=255)
     gCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGt                                                                                                                                              <  2:599045/151‑1 (MQ=255)
     gCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGt                                                                                                                                              >  1:795196/1‑151 (MQ=255)
      ccGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGtt                                                                                                                                             >  1:362182/1‑151 (MQ=255)
       cGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGttt                                                                                                                                            <  2:621239/151‑1 (MQ=255)
        ggTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           <  1:851383/151‑1 (MQ=255)
        ggTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           <  1:393230/151‑1 (MQ=255)
        ggTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           <  1:530235/151‑1 (MQ=255)
        ggTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           >  2:1087941/1‑151 (MQ=255)
         gTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           >  1:624384/1‑150 (MQ=255)
         gTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTc                                                                                                                                           >  2:422577/1‑150 (MQ=255)
          ttCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCAc                                                                                                                                         >  1:1177643/1‑151 (MQ=255)
            ccTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACcg                                                                                                                                       >  2:1444713/1‑151 (MQ=255)
            ccTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACcg                                                                                                                                       <  1:626051/151‑1 (MQ=255)
               tcttcCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACcgcgc                                                                                                                                    >  2:550087/1‑151 (MQ=255)
               tcttcCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACcgcg                                                                                                                                     <  1:1330777/150‑1 (MQ=255)
               tcttcCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACcgcg                                                                                                                                     <  2:586994/150‑1 (MQ=255)
                   ccTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCa                                                                                                                                 <  1:1341064/150‑1 (MQ=255)
                   ccTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCAc                                                                                                                                >  1:1023917/1‑151 (MQ=255)
                      tGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCa                                                                                                                              <  1:228610/150‑1 (MQ=255)
                        ccGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGgc                                                                                                                           <  1:1269137/151‑1 (MQ=255)
                         cGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGgcg                                                                                                                          >  2:516640/1‑151 (MQ=255)
                          gTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGgcgc                                                                                                                         >  1:165593/1‑151 (MQ=255)
                            cACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCt                                                                                                                        <  1:960761/150‑1 (MQ=255)
                             aCTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGt                                                                                                                      >  2:89232/1‑151 (MQ=255)
                             aCTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGt                                                                                                                      >  2:1199561/1‑151 (MQ=255)
                                 tttGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATAc                                                                                                                  >  1:1422330/1‑151 (MQ=255)
                                  ttGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGtat                                                                                                                    <  2:353675/148‑1 (MQ=255)
                                   tGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTCGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATAccc                                                                                                                >  1:859497/1‑151 (MQ=255)
                                    gAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATAccc                                                                                                                <  2:439470/150‑1 (MQ=255)
                                    gAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCca                                                                                                               <  2:1270815/151‑1 (MQ=255)
                                     aaGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCcat                                                                                                              <  1:1200245/151‑1 (MQ=255)
                                     aaGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCcat                                                                                                              >  1:325187/1‑151 (MQ=255)
                                      aGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCcatc                                                                                                             >  2:556899/1‑151 (MQ=255)
                                        aCGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCcatcat                                                                                                           <  1:886949/151‑1 (MQ=255)
                                           gATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCGTTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATctg                                                                                                        <  2:921317/151‑1 (MQ=255)
                                                tGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTgg                                                                                                    >  1:207540/1‑150 (MQ=255)
                                                 ggAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGa                                                                                                   <  2:1070699/150‑1 (MQ=255)
                                                  gAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGaaa                                                                                                 <  1:796773/151‑1 (MQ=255)
                                                  gAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGaaa                                                                                                 <  1:373902/151‑1 (MQ=255)
                                                       tACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCAcc                                                                                            >  2:1310024/1‑151 (MQ=255)
                                                        aCGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCAcc                                                                                            <  2:381963/150‑1 (MQ=255)
                                                        aCGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCg                                                                                           >  1:568575/1‑151 (MQ=255)
                                                        aCGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCg                                                                                           >  1:1224563/1‑151 (MQ=255)
                                                         cGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCTCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCg                                                                                           <  2:1080747/150‑1 (MQ=255)
                                                            tGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGAc                                                                                         >  1:664767/1‑149 (MQ=255)
                                                             gACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGc                                                                                      <  1:637510/151‑1 (MQ=255)
                                                             gACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGc                                                                                      >  1:794587/1‑151 (MQ=255)
                                                             gACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGc                                                                                      <  2:866572/151‑1 (MQ=255)
                                                              actactATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCg                                                                                     <  1:576104/151‑1 (MQ=255)
                                                                tactaTTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGAc                                                                                   >  1:1406846/1‑151 (MQ=255)
                                                                 actaTTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGa                                                                                    <  1:558214/149‑1 (MQ=255)
                                                                 actaTTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACt                                                                                  <  2:617141/151‑1 (MQ=255)
                                                                  ctaTTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTg                                                                                 >  2:1272507/1‑151 (MQ=255)
                                                                    aTTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTggg                                                                               >  2:819148/1‑151 (MQ=255)
                                                                         tgACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCAt                                                                           <  2:1375836/150‑1 (MQ=255)
                                                                          gACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCt                                                                         <  1:648343/151‑1 (MQ=255)
                                                                                  tCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGtt                                                                 >  1:444132/1‑151 (MQ=255)
                                                                                   cTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGtt                                                                 >  1:1344093/1‑150 (MQ=255)
                                                                                     gCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGtt                                                                 <  2:165573/148‑1 (MQ=255)
                                                                                     gCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAAc                                                              <  1:510351/151‑1 (MQ=255)
                                                                                     gCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAAc                                                              <  2:612955/151‑1 (MQ=255)
                                                                                       cGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCt                                                            <  2:936622/151‑1 (MQ=255)
                                                                                        ggTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTg                                                           <  1:138845/151‑1 (MQ=255)
                                                                                         gTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGa                                                          <  2:426373/151‑1 (MQ=255)
                                                                                            gcccgcccGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATg                                                        <  1:1069757/150‑1 (MQ=255)
                                                                                            gcccgcccGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGc                                                       >  1:191633/1‑151 (MQ=255)
                                                                                            gcccgcccGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGc                                                       <  2:1420887/151‑1 (MQ=255)
                                                                                             cccgcccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGcc                                                      >  1:1326461/1‑151 (MQ=255)
                                                                                                gcccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAgg                                                   >  2:359525/1‑151 (MQ=255)
                                                                                                gcccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAg                                                    >  1:193013/1‑150 (MQ=255)
                                                                                                  ccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGGTGCCAGGCa                                                 <  2:795118/151‑1 (MQ=255)
                                                                                                       aTGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGAc                                            >  2:753095/1‑151 (MQ=255)
                                                                                                           gATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACcgcg                                        >  2:1358877/1‑151 (MQ=255)
                                                                                                            aTCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGt                                       >  2:91759/1‑151 (MQ=255)
                                                                                                             tCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCGCCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGt                                       <  1:390488/150‑1 (MQ=255)
                                                                                                                 tctcGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTg                                   >  2:758553/1‑150 (MQ=255)
                                                                                                                   tcGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATc                                >  1:1394479/1‑151 (MQ=255)
                                                                                                                    cGTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATc                                <  2:719318/150‑1 (MQ=255)
                                                                                                                     gTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCg                               >  1:1036894/1‑150 (MQ=255)
                                                                                                                     gTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGa                              <  2:541210/151‑1 (MQ=255)
                                                                                                                     gTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGa                              <  1:1288985/151‑1 (MQ=255)
                                                                                                                     gTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGa                              <  2:118232/151‑1 (MQ=255)
                                                                                                                     gTCTGGAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGa                              <  1:768229/151‑1 (MQ=255)
                                                                                                                         ggAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGaaaa                           <  1:477148/150‑1 (MQ=255)
                                                                                                                         ggAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGaaa                            <  1:422633/149‑1 (MQ=255)
                                                                                                                          gAATCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGaa                             <  1:1350472/147‑1 (MQ=255)
                                                                                                                           aaTCACGCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCt                        <  1:599207/151‑1 (MQ=255)
                                                                                                                                 gCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGaaaaa                  <  1:1437099/151‑1 (MQ=255)
                                                                                                                                 gCATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGaaaa                   >  1:524237/1‑150 (MQ=255)
                                                                                                                                  cATTGTGCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGaaaaaa                 <  2:937814/151‑1 (MQ=255)
                                                                                                                                     tgtgCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACa               <  2:160038/150‑1 (MQ=255)
                                                                                                                                      gtgCATCTCGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAg              <  1:1088039/150‑1 (MQ=255)
                                                                                                                                              cGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAActgct      <  1:1193451/150‑1 (MQ=255)
                                                                                                                                              cGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAActg        <  1:786357/148‑1 (MQ=255)
                                                                                                                                              cGGTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAAct         <  2:722436/147‑1 (MQ=255)
                                                                                                                                               ggTTGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGt    >  2:18087/1‑151 (MQ=255)
                                                                                                                                                 ttGTGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTac  >  2:1392117/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTAC  >  NC_000913/4552842‑4553137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: