Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 474,819 A→G F115L (TTC→CTC)  tesB ← acyl‑CoA thioesterase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913474,8190AG98.9% 358.8 / NA 94F115L (TTC→CTC) tesBacyl‑CoA thioesterase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (43/50);  minor base C (1/0);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCG  >  NC_000913/474671‑474966
                                                                                                                                                    |                                                                                                                                                   
tCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGAc                                                                                                                                                   >  2:1416093/1‑151 (MQ=255)
 ccACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGAcc                                                                                                                                                  <  2:1332606/151‑1 (MQ=255)
  cACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGAccc                                                                                                                                                 <  1:1265949/151‑1 (MQ=255)
   aCCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCg                                                                                                                                                >  1:56690/1‑151 (MQ=255)
    ccGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGc                                                                                                                                               >  2:1131496/1‑151 (MQ=255)
     cggacggaCTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCt                                                                                                                                              <  1:1033228/151‑1 (MQ=255)
     cggacggaCTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCt                                                                                                                                              >  1:398028/1‑151 (MQ=255)
      ggacggacTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCtt                                                                                                                                             >  2:274978/1‑151 (MQ=255)
      ggacggacTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCtt                                                                                                                                             <  1:207706/151‑1 (MQ=255)
               tCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGc                                                                                                                                      <  1:819084/149‑1 (MQ=255)
                  aGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTgg                                                                                                                                  <  2:409570/150‑1 (MQ=255)
                   gCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGaa                                                                                                                                <  2:661618/151‑1 (MQ=255)
                   gCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGa                                                                                                                                 >  2:966009/1‑150 (MQ=255)
                   gCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGa                                                                                                                                 >  1:1432039/1‑150 (MQ=255)
                    cGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGaaa                                                                                                                               <  2:1416808/151‑1 (MQ=255)
                        cGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAagag                                                                                                                            <  1:703588/150‑1 (MQ=255)
                        cGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAgg                                                                                                                           >  1:1315190/1‑151 (MQ=255)
                        cGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAgg                                                                                                                           >  1:66766/1‑151 (MQ=255)
                           tCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGc                                                                                                                          >  2:1134988/1‑149 (MQ=255)
                           tCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGc                                                                                                                          >  2:304243/1‑149 (MQ=255)
                            cGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGc                                                                                                                          <  1:1223572/148‑1 (MQ=255)
                            cGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAg                                                                                                                        <  1:686249/150‑1 (MQ=255)
                            cGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGt                                                                                                                       >  2:1182096/1‑151 (MQ=255)
                             gCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTc                                                                                                                      >  1:483881/1‑151 (MQ=255)
                              cAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTc                                                                                                                      >  2:1288372/1‑150 (MQ=255)
                              cAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTc                                                                                                                      >  1:313793/1‑150 (MQ=255)
                                  tGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCatat                                                                                                                  >  1:736680/1‑150 (MQ=255)
                                   gAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATaa                                                                                                                <  2:1449145/151‑1 (MQ=255)
                                     aTTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATaaa                                                                                                               >  1:1464831/1‑150 (MQ=255)
                                       ttATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATaaaaa                                                                                                             >  2:205595/1‑150 (MQ=255)
                                        tataTTTCAGCATTGGCGGCAGCAGGTGAGCCAGCGATTGGGAGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGTGCGGACGGCATTGTTTTTTGATGTTCGTGCCCCGCTTTTGGTGCCTGGAAAGAGGCAGTCATATaaaaaa                                                                                                            <  1:463091/150‑1 (MQ=255)
                                              tCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATc                                                                                                          <  2:372458/146‑1 (MQ=255)
                                               cAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGttt                                                                                                     <  2:816837/150‑1 (MQ=255)
                                               cAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGttt                                                                                                     <  1:908113/150‑1 (MQ=255)
                                               cAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTg                                                                                                    >  1:1400621/1‑151 (MQ=255)
                                                aGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGc                                                                                                   >  1:1244634/1‑151 (MQ=255)
                                                aGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGc                                                                                                   <  1:1267841/151‑1 (MQ=255)
                                                     tGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGttt                                                                                              >  1:1291620/1‑151 (MQ=255)
                                                      ggcggcAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGtttt                                                                                             >  1:384827/1‑151 (MQ=255)
                                                      ggcggcAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGtttt                                                                                             <  2:470984/151‑1 (MQ=255)
                                                        cggcAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTg                                                                                            >  1:1179237/1‑150 (MQ=255)
                                                          gcagcaGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAAt                                                                                         <  2:21017/151‑1 (MQ=255)
                                                            agcagGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAAt                                                                                         >  2:365927/1‑149 (MQ=255)
                                                             gcagGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATag                                                                                       <  2:1440017/150‑1 (MQ=255)
                                                               agGTGCGCCAGCGATTGGGCGTTTTGCGTTTCCGAAGGGAGGCCATCAGGCGCGGGCGCGGACGGCATTGTTTTTTGATTTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATagc                                                                                      <  1:294340/149‑1 (MQ=255)
                                                                ggTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATagcagc                                                                                   <  1:963476/151‑1 (MQ=255)
                                                                 gTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAgcagca                                                                                  <  1:823290/151‑1 (MQ=255)
                                                                 gTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAgcagca                                                                                  <  1:462192/151‑1 (MQ=255)
                                                                   gcgcCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAAc                                                                                >  1:546863/1‑151 (MQ=255)
                                                                    cgcCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGACACCTGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAAc                                                                                >  1:849974/1‑150 (MQ=255)
                                                                       cAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcgg                                                                            <  2:202145/151‑1 (MQ=255)
                                                                       cAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcgg                                                                            >  1:1023582/1‑151 (MQ=255)
                                                                       cAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcgg                                                                            >  1:42721/1‑151 (MQ=255)
                                                                         gCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcggcg                                                                          >  2:782698/1‑151 (MQ=255)
                                                                         gCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcggc                                                                           <  2:686089/150‑1 (MQ=255)
                                                                           gATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcggcgg                                                                         <  2:225072/150‑1 (MQ=255)
                                                                            aTTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCggcgggc                                                                       <  1:130681/151‑1 (MQ=255)
                                                                            aTTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCggcgggc                                                                       >  1:258945/1‑151 (MQ=255)
                                                                            aTTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCggcgggc                                                                       >  1:704466/1‑151 (MQ=255)
                                                                            aTTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCggcgggc                                                                       >  2:126421/1‑151 (MQ=255)
                                                                              tGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCggcgggcg                                                                      <  1:1131594/150‑1 (MQ=255)
                                                                               gggCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCt                                                                    <  2:724501/151‑1 (MQ=255)
                                                                                 gCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTg                                                                   >  1:558944/1‑150 (MQ=255)
                                                                                         cGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTa                                                          <  1:1182194/151‑1 (MQ=255)
                                                                                            ttCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTAcc                                                        <  2:346460/150‑1 (MQ=255)
                                                                                             tCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCGTATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCg                                                       <  2:548707/150‑1 (MQ=255)
                                                                                                gAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCa                                                    >  1:408124/1‑150 (MQ=255)
                                                                                                gAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCAc                                                   >  2:1168721/1‑151 (MQ=255)
                                                                                                   gggAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCa                                                <  1:481520/151‑1 (MQ=255)
                                                                                                     gAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGc                                              <  2:1212854/151‑1 (MQ=255)
                                                                                                       ggCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGt                                            >  1:525950/1‑151 (MQ=255)
                                                                                                           aTCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCg                                        <  1:1187187/151‑1 (MQ=255)
                                                                                                             cAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGa                                       <  1:1153712/150‑1 (MQ=255)
                                                                                                             cAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGACTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGa                                       <  2:60884/150‑1 (MQ=255)
                                                                                                               ggCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGAca                                     <  1:255746/150‑1 (MQ=255)
                                                                                                                 cgcTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGAcatca                                  <  1:140700/151‑1 (MQ=255)
                                                                                                                  gcTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGAcatcat                                 >  1:1172375/1‑151 (MQ=255)
                                                                                                                   cTGGCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGAcatcat                                 >  2:1039912/1‑150 (MQ=255)
                                                                                                                     ggCGCGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCataa                               <  1:275009/150‑1 (MQ=255)
                                                                                                                      gcgcGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCataaat                             <  2:163695/151‑1 (MQ=255)
                                                                                                                      gcgcGGACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCataaa                              >  1:1179390/1‑150 (MQ=255)
                                                                                                                          ggACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATc                         >  1:1340566/1‑151 (MQ=255)
                                                                                                                           gACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCg                        >  2:1461320/1‑151 (MQ=255)
                                                                                                                           gACGGCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCg                        <  2:684330/151‑1 (MQ=255)
                                                                                                                              ggCATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGct                     <  2:634569/151‑1 (MQ=255)
                                                                                                                                cATTGTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGctt                    >  2:1358318/1‑150 (MQ=255)
                                                                                                                                    gTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTa                <  1:966102/150‑1 (MQ=255)
                                                                                                                                    gTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTa                <  2:56683/150‑1 (MQ=255)
                                                                                                                                    gTTTTTTGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTAc               >  1:742948/1‑151 (MQ=255)
                                                                                                                                       ttttGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTa             <  2:313756/150‑1 (MQ=255)
                                                                                                                                        tttGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAACCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTCAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTAt            <  2:76911/150‑1 (MQ=255)
                                                                                                                                         ttGATGTTCGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCg          >  1:506390/1‑151 (MQ=255)
                                                                                                                                                 cGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAGCCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCg  <  2:397972/151‑1 (MQ=255)
                                                                                                                                                 cGAGACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGc   <  1:866162/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCG  >  NC_000913/474671‑474966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑