Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 60,012 T→A D112V (GAT→GTT)  rluA ← dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091360,0120TA98.8% 323.3 / NA 85D112V (GAT→GTT) rluAdual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (42/42);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCA  >  NC_000913/59865‑60159
                                                                                                                                                   |                                                                                                                                                   
gAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAAcca                                                                                                                                                  <  1:668536/151‑1 (MQ=255)
 aaCGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAAccac                                                                                                                                                 >  1:654193/1‑151 (MQ=255)
 aaCGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAAccac                                                                                                                                                 >  1:1097284/1‑151 (MQ=255)
   cGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAAccacca                                                                                                                                               <  2:1358534/151‑1 (MQ=255)
    gCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAaccacc                                                                                                                                                >  1:879943/1‑149 (MQ=255)
    gCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAg                                                                                                                                              <  2:1163138/151‑1 (MQ=255)
     cccGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAaccacc                                                                                                                                                >  2:850399/1‑148 (MQ=255)
       cGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGAcc                                                                                                                                           >  2:957765/1‑151 (MQ=255)
            aTCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTg                                                                                                                                      <  2:44325/151‑1 (MQ=255)
            aTCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTg                                                                                                                                      <  2:256824/151‑1 (MQ=255)
              cGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACAGACCTTCTGCgg                                                                                                                                   >  2:367792/1‑151 (MQ=255)
                  tttAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCgggg                                                                                                                                 <  2:1098573/150‑1 (MQ=255)
                  tttAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCgggg                                                                                                                                 <  2:922910/150‑1 (MQ=255)
                   ttAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCggggg                                                                                                                                >  1:102689/1‑150 (MQ=255)
                    tAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCTGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGgga                                                                                                                               <  2:826747/150‑1 (MQ=255)
                         ccacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGggatggat                                                                                                                          <  2:229673/150‑1 (MQ=255)
                         ccacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGgatggatg                                                                                                                         >  2:1148415/1‑151 (MQ=255)
                           acTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGcc                                                                                                                       >  2:1133865/1‑151 (MQ=255)
                           acTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGcc                                                                                                                       >  1:251054/1‑151 (MQ=255)
                                   cGTGTTATCCGCCGCATACTCCACCACTTCATACTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGAcg                                                                                                               >  1:20203/1‑151 (MQ=255)
                                    gtgtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGAcgc                                                                                                              <  2:768515/151‑1 (MQ=255)
                                      gtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCgg                                                                                                            <  1:1135263/151‑1 (MQ=255)
                                          tCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGcc                                                                                                         >  2:363066/1‑150 (MQ=255)
                                              ccgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACAt                                                                                                     >  2:935991/1‑150 (MQ=255)
                                              ccgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATa                                                                                                    <  2:824455/151‑1 (MQ=255)
                                              ccgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATa                                                                                                    <  2:423733/151‑1 (MQ=255)
                                               cgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATAc                                                                                                   >  2:538431/1‑151 (MQ=255)
                                                gcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACt                                                                                                  >  2:317151/1‑151 (MQ=255)
                                                gcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACt                                                                                                  >  2:600832/1‑151 (MQ=255)
                                                       ccaccaCTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCtttt                                                                                            <  1:781803/150‑1 (MQ=255)
                                                          ccacTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCgg                                                                                        <  2:512817/151‑1 (MQ=255)
                                                           cacTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgc                                                                                       <  1:800096/151‑1 (MQ=255)
                                                              ttCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTTGgctc                                                                                     >  1:1151065/1‑150 (MQ=255)
                                                                  tatTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcg                                                                                  <  1:1215889/149‑1 (MQ=255)
                                                                  tatTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgct                                                                                >  1:737352/1‑151 (MQ=255)
                                                                  tatTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgc                                                                                 >  2:90017/1‑150 (MQ=255)
                                                                  tatTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcg                                                                                  >  2:1104608/1‑149 (MQ=255)
                                                                   atTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctc                                                                               >  1:1384585/1‑151 (MQ=255)
                                                                   atTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctc                                                                               <  2:1287891/151‑1 (MQ=255)
                                                                    ttCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGGCTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGGCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctc                                                                               <  2:638108/150‑1 (MQ=255)
                                                                    ttCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctcg                                                                              >  1:533424/1‑151 (MQ=255)
                                                                     tCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctcgc                                                                             >  2:949385/1‑151 (MQ=255)
                                                                     tCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctcg                                                                              <  2:1449620/150‑1 (MQ=255)
                                                                       cGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCgg                                                                           <  1:336609/151‑1 (MQ=255)
                                                                       cGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCgg                                                                           <  1:316693/151‑1 (MQ=255)
                                                                       cGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCgg                                                                           >  2:176512/1‑151 (MQ=255)
                                                                        gTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGa                                                                          <  2:651168/151‑1 (MQ=255)
                                                                         tCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGa                                                                          >  2:824202/1‑150 (MQ=255)
                                                                            gcgcAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGaa                                                                         <  2:1238047/148‑1 (MQ=255)
                                                                            gcgcAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAAc                                                                        <  2:998429/149‑1 (MQ=255)
                                                                            gcgcAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTg                                                                      >  1:833642/1‑151 (MQ=255)
                                                                             cgcAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTgg                                                                     <  1:1242911/151‑1 (MQ=255)
                                                                              gcAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGc                                                                    >  1:422990/1‑151 (MQ=255)
                                                                               cAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCg                                                                   <  2:859768/151‑1 (MQ=255)
                                                                                aGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTg                                                                      >  2:184081/1‑147 (MQ=255)
                                                                                 ggTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGtt                                                                 <  2:200004/151‑1 (MQ=255)
                                                                                 ggTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGtt                                                                 <  1:1021064/151‑1 (MQ=255)
                                                                                  gTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGttt                                                                >  1:850697/1‑151 (MQ=255)
                                                                                     tACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTa                                                              <  1:317189/150‑1 (MQ=255)
                                                                                      aCCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCGCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAAc                                                            <  1:440390/151‑1 (MQ=255)
                                                                                        ccGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTc                                                          >  2:750716/1‑151 (MQ=255)
                                                                                               gTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTcc                                                   >  2:83821/1‑151 (MQ=255)
                                                                                                   cAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCgg                                               <  1:1185092/151‑1 (MQ=255)
                                                                                                   cAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTACCGCTCCGCgg                                               >  2:827032/1‑151 (MQ=255)
                                                                                                      aCTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCtt                                            >  2:917961/1‑151 (MQ=255)
                                                                                                        tttCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCttt                                           >  1:1310999/1‑150 (MQ=255)
                                                                                                         ttCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCttt                                           <  2:1079645/149‑1 (MQ=255)
                                                                                                         ttCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCttt                                           <  2:327076/149‑1 (MQ=255)
                                                                                                         ttCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTg                                          <  1:1461171/150‑1 (MQ=255)
                                                                                                            tGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTc                                       >  1:197586/1‑150 (MQ=255)
                                                                                                              tttCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgc                                    >  1:404958/1‑151 (MQ=255)
                                                                                                                 cGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGcc                                 <  1:850267/151‑1 (MQ=255)
                                                                                                                     cGGTTTGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACt                              >  2:598542/1‑150 (MQ=255)
                                                                                                                        tttGGCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                            >  1:909042/1‑149 (MQ=255)
                                                                                                                            gCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAc                      <  1:585644/151‑1 (MQ=255)
                                                                                                                            gCCAGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTGTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAc                      <  1:781731/151‑1 (MQ=255)
                                                                                                                               aGTCGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgcc                   >  2:1285559/1‑151 (MQ=255)
                                                                                                                                  cGCAAATCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCt                <  1:1148511/151‑1 (MQ=255)
                                                                                                                                     aaaTCAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg              >  2:942945/1‑150 (MQ=255)
                                                                                                                                         cAGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc         >  1:111415/1‑151 (MQ=255)
                                                                                                                                          aGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc         >  2:891881/1‑150 (MQ=255)
                                                                                                                                          aGCGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCa        <  1:1133963/151‑1 (MQ=255)
                                                                                                                                           gCGGCAGAACCACAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCata      <  1:367842/151‑1 (MQ=255)
                                                                                                                                            cGGCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCat       >  2:159334/1‑150 (MQ=255)
                                                                                                                                             ggCAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCatat     >  1:1302429/1‑151 (MQ=255)
                                                                                                                                               cAGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATcc   <  2:77399/151‑1 (MQ=255)
                                                                                                                                                aGAACCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCa  <  1:538493/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCA  >  NC_000913/59865‑60159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: