Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,014	A→G	E268G (GAA→GGA)	djlC →	J domain‑containing HscC co‑chaperone; Hsc56

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,014	0	A	G	100.0%	262.4 / NA	69	E268G (GAA→GGA)	djlC	J domain‑containing HscC co‑chaperone; Hsc56
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/34);  total (35/34)

CATCGCAAACTGTTGCGCTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGAAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCATGGTAGATG  >  NC_000913/680866‑681160                                                                                                                                                     |                                                                                                                                                   cATCGCAAACTGTTGCGCTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGgaa                                                                                                                                                  <  2:197998/151‑1 (MQ=255)              tGCGCTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTTCTGTCGCTCCACAGTGGCAAACGGAAGAACCAGAAAGCGAAGGTGACCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGcc                                                                                                                                       >  2:1193939/1‑149 (MQ=255)               gcgcTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGAt                                                                                                                                    >  1:500716/1‑151 (MQ=255)               gcgcTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTAATTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGa                                                                                                                                     >  1:757347/1‑150 (MQ=255)                cgcTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGAt                                                                                                                                    >  2:1182033/1‑150 (MQ=255)                  cTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGGGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGAtctc                                                                                                                                 >  2:987063/1‑151 (MQ=255)                   tGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATctct                                                                                                                                >  2:423704/1‑151 (MQ=255)                   tGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCCCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGAtctc                                                                                                                                 >  2:1250905/1‑150 (MQ=255)                    ggTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATctct                                                                                                                                >  1:467595/1‑150 (MQ=255)                       aCAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTgcgc                                                                                                                            <  1:1293443/151‑1 (MQ=255)                        cAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGcgcg                                                                                                                           >  1:325466/1‑151 (MQ=255)                        cAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGcgcg                                                                                                                           <  2:608174/151‑1 (MQ=255)                           cTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTAc                                                                                                                        >  1:774692/1‑151 (MQ=255)                             cGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACt                                                                                                                       >  2:1460700/1‑150 (MQ=255)                             cGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTg                                                                                                                      <  2:921135/151‑1 (MQ=255)                              ggTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTAc                                                                                                                        <  2:584506/148‑1 (MQ=255)                              ggTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTgg                                                                                                                     <  1:1306199/151‑1 (MQ=255)                               gTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGGGGCAAACGGAAAAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAAAGGTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTggg                                                                                                                    >  2:107874/1‑151 (MQ=255)                                tGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTggg                                                                                                                    >  1:27295/1‑150 (MQ=255)                                 gCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGa                                                                                                                   <  1:1072776/150‑1 (MQ=255)                                  cAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGaaa                                                                                                                 <  2:120144/151‑1 (MQ=255)                                   aaaCAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAAAGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGaa                                                                                                                  >  1:1185766/1‑149 (MQ=255)                                    aaCAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGt                                                                                                               >  1:1436755/1‑151 (MQ=255)                                     aCAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGAGGCAAACGGGAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAAAGTTTTTACTGCGGCGCGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAAtt                                                                                                              >  2:824227/1‑151 (MQ=255)                                             ggCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATc                                                                                                       <  1:803670/150‑1 (MQ=255)                                             ggCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCt                                                                                                      >  1:234183/1‑151 (MQ=255)                                              gCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTa                                                                                                     <  2:948862/151‑1 (MQ=255)                                              gCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTa                                                                                                     <  1:1010538/151‑1 (MQ=255)                                                ggAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTac                                                                                                    >  1:102778/1‑150 (MQ=255)                                                      cATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGc                                                                                              <  2:124041/150‑1 (MQ=255)                                                      cATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCt                                                                                             <  2:651988/151‑1 (MQ=255)                                                       aTTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTg                                                                                            <  2:149077/151‑1 (MQ=255)                                                       aTTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTg                                                                                            <  2:1042242/151‑1 (MQ=255)                                                                   cAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATttgttgt                                                                                <  1:1337168/151‑1 (MQ=255)                                                                    aaCAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATttgttgtt                                                                               <  1:801237/151‑1 (MQ=255)                                                                     aCAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATttgttgttg                                                                              <  2:186810/151‑1 (MQ=255)                                                                     aCAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATttgttgttg                                                                              <  1:843655/151‑1 (MQ=255)                                                                     aCAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATttgttgttg                                                                              >  2:622453/1‑151 (MQ=255)                                                                      cAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGc                                                                             >  2:726412/1‑151 (MQ=255)                                                                         tGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGt                                                                          >  1:642719/1‑151 (MQ=255)                                                                         tGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGt                                                                          >  2:1278450/1‑151 (MQ=255)                                                                          ggCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTg                                                                         <  2:1372435/151‑1 (MQ=255)                                                                            cAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGa                                                                       <  2:296439/151‑1 (MQ=255)                                                                             aaaCGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTgg                                                                        >  2:868349/1‑149 (MQ=255)                                                                                    agaaCCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGc                                                                 <  1:468849/149‑1 (MQ=255)                                                                                              aGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCgg                                                     <  2:1413880/151‑1 (MQ=255)                                                                                                cGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGat                                                   <  1:1289993/151‑1 (MQ=255)                                                                                                 gAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGat                                                   >  2:646013/1‑150 (MQ=255)                                                                                                  aaGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGatt                                                  >  1:389112/1‑150 (MQ=255)                                                                                                   aGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGattat                                                >  1:924907/1‑151 (MQ=255)                                                                                                      tGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTc                                             >  1:1223544/1‑151 (MQ=255)                                                                                                       gCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTcg                                            >  2:505586/1‑151 (MQ=255)                                                                                                       gCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTcg                                            <  1:1251004/151‑1 (MQ=255)                                                                                                       gCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTcg                                            <  2:953349/151‑1 (MQ=255)                                                                                                       gCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTc                                             <  1:976272/150‑1 (MQ=255)                                                                                                          cGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGt                                         >  1:572327/1‑151 (MQ=255)                                                                                                             tATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGttat                                      >  1:95705/1‑151 (MQ=255)                                                                                                               ttACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACTCAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGttatt                                     <  1:152656/150‑1 (MQ=255)                                                                                                                tACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGttatta                                    <  2:1159275/150‑1 (MQ=255)                                                                                                                    tgtgtgCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGAt                               >  1:1218018/1‑151 (MQ=255)                                                                                                                       gtgCACAACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGt                            >  1:318848/1‑151 (MQ=255)                                                                                                                          cacaACGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTAt                         <  1:1112670/151‑1 (MQ=255)                                                                                                                             aaCGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGa                      <  2:1197345/151‑1 (MQ=255)                                                                                                                               cGTTTGTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAg                    <  1:423760/151‑1 (MQ=255)                                                                                                                                    gTACTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCgg               >  1:190246/1‑151 (MQ=255)                                                                                                                                      aCTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCgg               >  1:1098361/1‑149 (MQ=255)                                                                                                                                      aCTGCGGCGAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCgg               >  1:1090939/1‑149 (MQ=255)                                                                                                                                         gcggcgAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCa           <  1:505643/150‑1 (MQ=255)                                                                                                                                             cgAGGGGGGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCATGGTa      <  1:1194038/151‑1 (MQ=255)                                                                                                                                                ggggggAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCATGGTAGAt   <  2:467540/151‑1 (MQ=255)                                                                                                                                                 ggggGAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCATGGTAGATg  <  2:1169243/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CATCGCAAACTGTTGCGCTGGTACAGCTCGGTGCAAACAGGTATGGCGGAACTCATTCCTGTCGCTCAACAGTGGCAAACGGAAGAACCAGAAAGCGAAGATGCCCGGTATTACTTGTGTGCACAACGTTTGTACTGCGGCGAGGGGGAAAGCCTGCTTGCCGATCTCTGCGCGTACTGGGAAAGTTACCCATCTACACAAGCTGATAATTTGTTGTTGCAGTGGAGCAAGCGTCATTGCCCGGATTATTTCGCGTTATTAGTGATGGTTATCGAAGCGCGGAGCATGGTAGATG  >  NC_000913/680866‑681160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑