Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,440	C→A	A364S (GCG→TCG)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,440	0	C	A	100.0%	399.1 / NA	100	A364S (GCG→TCG)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/59);  total (41/59)

GCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTG  >  NC_000913/82292‑82586                                                                                                                                                     |                                                                                                                                                   gCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACa                                                                                                                                                  <  1:831426/151‑1 (MQ=255)   cAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGc                                                                                                                                                <  1:1094130/151‑1 (MQ=255)    aGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCt                                                                                                                                               >  1:1100195/1‑151 (MQ=255)    aGTTTGACGAAGGCGGTGGCGCAATCGTTAGAGCCAGACTGCACGCTGAAGAAATCCAGACGGAAATGGTCCGGCTCTTATTGCTGATTACCGAAGAAGGTCAGCGCCTCCAGATCGTAATCAACCAACTGCCCTTTTTTGTCCGACAGCt                                                                                                                                               >  2:1178959/1‑151 (MQ=255)      tttGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCtt                                                                                                                                              <  2:745090/150‑1 (MQ=255)           cggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGaa                                                                                                                                        <  1:133428/151‑1 (MQ=255)           cggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGa                                                                                                                                         >  2:593705/1‑150 (MQ=255)             gcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAg                                                                                                                                      <  1:1061865/151‑1 (MQ=255)             gcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAg                                                                                                                                      <  1:1422633/151‑1 (MQ=255)                 cggTGGGGATATAGTTAAAGCCAGACGGCACGCTGAAGTAATCCAGAAGGAAATGCTAAGGCCCTTCTTGCTGCTTACCGATGAAGTTCAGCGTCTCCAGAACGTAATCAAATGCCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGcct                                                                                                                                   >  2:922768/1‑148 (MQ=255)                 cggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATc                                                                                                                                  <  1:658498/151‑1 (MQ=255)                 cggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATc                                                                                                                                  >  2:1450081/1‑151 (MQ=255)                     ggCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTa                                                                                                                               <  1:452820/150‑1 (MQ=255)                     ggCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTa                                                                                                                               <  2:821742/150‑1 (MQ=255)                       cGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACa                                                                                                                             >  2:604670/1‑150 (MQ=255)                        gATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACaaa                                                                                                                           <  2:462449/151‑1 (MQ=255)                         atatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAAt                                                                                                                          >  1:1101593/1‑151 (MQ=255)                         atatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAAt                                                                                                                          <  2:72573/151‑1 (MQ=255)                           atCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAAtt                                                                                                                         <  1:821950/150‑1 (MQ=255)                           atCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAAtt                                                                                                                         <  1:527208/150‑1 (MQ=255)                             cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTc                                                                                                                      >  1:758983/1‑151 (MQ=255)                             cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTc                                                                                                                      <  2:392934/151‑1 (MQ=255)                              gTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                                                                                     <  2:1028139/151‑1 (MQ=255)                              gTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                                                                                     <  1:1181891/151‑1 (MQ=255)                               ttAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                                                                                     <  2:271519/150‑1 (MQ=255)                                  gagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAt                                                                                                                 <  2:1324750/151‑1 (MQ=255)                                   agCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAtt                                                                                                                <  1:699442/151‑1 (MQ=255)                                   agCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAt                                                                                                                 <  1:1260608/150‑1 (MQ=255)                                        gACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATAtt                                                                                                           <  1:1040893/151‑1 (MQ=255)                                            gCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCa                                                                                                         <  1:1425345/149‑1 (MQ=255)                                             cACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActt                                                                                                      <  1:806152/151‑1 (MQ=255)                                             cACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActt                                                                                                      >  2:1156678/1‑151 (MQ=255)                                              aCGCTGAAGAAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                                                                                     >  2:1036467/1‑151 (MQ=255)                                               cGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttc                                                                                                    <  2:1468807/151‑1 (MQ=255)                                               cGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttc                                                                                                    <  2:174741/151‑1 (MQ=255)                                                  tGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                                                                                                 >  2:202102/1‑151 (MQ=255)                                                   gAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                                                                                                  <  2:278004/149‑1 (MQ=255)                                                   gAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTta                                                                                                <  1:271536/151‑1 (MQ=255)                                                    aaGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtat                                                                                               <  2:1186010/151‑1 (MQ=255)                                                      gTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAc                                                                                             >  1:1068470/1‑151 (MQ=255)                                                       tAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcc                                                                                            >  2:1338687/1‑151 (MQ=255)                                                         aTCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcccc                                                                                          <  2:1083775/151‑1 (MQ=255)                                                           ccAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAt                                                                                        >  1:1158004/1‑151 (MQ=255)                                                            cAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGTCCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCagc                                                                                       <  1:558187/151‑3 (MQ=255)                                                            cAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtc                                                                                       <  2:332172/151‑1 (MQ=255)                                                             aGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtct                                                                                      >  2:411760/1‑151 (MQ=255)                                                               aCGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCGtctc                                                                                     >  2:1075957/1‑150 (MQ=255)                                                               aCGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCa                                                                                    <  2:1286585/151‑1 (MQ=255)                                                               aCGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCa                                                                                    <  2:915181/151‑1 (MQ=255)                                                                cGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCAt                                                                                   <  1:726840/151‑1 (MQ=255)                                                                 ggAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc                                                                                  <  1:616068/151‑1 (MQ=255)                                                                 ggAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc                                                                                  >  2:1272608/1‑151 (MQ=255)                                                                  gAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATcc                                                                                 <  1:240990/151‑1 (MQ=255)                                                                    aaTGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt                                                                               <  2:651917/151‑1 (MQ=255)                                                                    aaTGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt                                                                               <  1:460719/151‑1 (MQ=255)                                                                     aTGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATg                                                                              <  1:1331184/151‑1 (MQ=255)                                                                      tGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGc                                                                             >  2:505709/1‑151 (MQ=255)                                                                       gCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGc                                                                             <  2:776511/150‑1 (MQ=255)                                                                            ggCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGtt                                                                       >  1:1297918/1‑151 (MQ=255)                                                                            ggCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGtt                                                                       >  1:1200368/1‑151 (MQ=255)                                                                                cttcttGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCAc                                                                   >  2:1430900/1‑151 (MQ=255)                                                                                cttcttGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCAc                                                                   >  2:904097/1‑151 (MQ=255)                                                                                    ttGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCAccgcc                                                               >  2:1033970/1‑151 (MQ=255)                                                                                         gctTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCAc                                                           <  1:1188151/150‑1 (MQ=255)                                                                                         gctTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACg                                                          <  1:1074082/151‑1 (MQ=255)                                                                                           ttACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGcc                                                        >  2:1105057/1‑151 (MQ=255)                                                                                           ttACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGcc                                                        >  1:269805/1‑151 (MQ=255)                                                                                              ccGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCg                                                     >  2:976931/1‑151 (MQ=255)                                                                                               cGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCTCCGCCGCACGCCCCGa                                                    <  1:1425961/151‑1 (MQ=255)                                                                                                  tGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACg                                                 >  2:1247891/1‑151 (MQ=255)                                                                                                   gAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCCCTTTCTTTATACCCCATCTCATCCAGGCGATGTTTCACCGCCGCACGCCCCGAACg                                                 <  2:138882/150‑1 (MQ=255)                                                                                                     aGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACgaga                                              <  1:56614/151‑1 (MQ=255)                                                                                                     aGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACg                                                 >  2:451680/1‑148 (MQ=255)                                                                                                          aGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTc                                          >  1:856189/1‑150 (MQ=255)                                                                                                           gcgcCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTc                                          >  2:1315201/1‑149 (MQ=255)                                                                                                             gcCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTc                                          >  2:836315/1‑147 (MQ=255)                                                                                                              ccTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGAtt                                     <  1:202124/151‑1 (MQ=255)                                                                                                              ccTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGAtt                                     >  1:110124/1‑151 (MQ=255)                                                                                                              ccTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGAt                                      <  1:219704/150‑1 (MQ=255)                                                                                                               cTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTc                                    >  1:1472961/1‑151 (MQ=255)                                                                                                                  cAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGc                                 <  2:1100351/151‑1 (MQ=255)                                                                                                                  cAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGc                                 >  2:667106/1‑151 (MQ=255)                                                                                                                  cAGATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGc                                 <  1:63289/151‑1 (MQ=255)                                                                                                                    gATCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTg                               <  1:1428458/151‑1 (MQ=255)                                                                                                                    aaTCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTg                               >  1:107555/2‑151 (MQ=255)                                                                                                                     aTCGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTgg                              >  2:720849/1‑151 (MQ=255)                                                                                                                       cGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGa                             <  2:510207/150‑1 (MQ=255)                                                                                                                       cGTAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGAt                            >  2:338959/1‑151 (MQ=255)                                                                                                                         tAATCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGAtt                           <  2:1329614/150‑1 (MQ=255)                                                                                                                          aaTCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGAttt                          <  1:89353/150‑1 (MQ=255)                                                                                                                          aaTCAAACACCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTg                         >  1:354990/1‑151 (MQ=255)                                                                                                                                acacCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAg                   <  2:1191882/151‑1 (MQ=255)                                                                                                                                 cacCTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGa                  <  1:1450188/151‑1 (MQ=255)                                                                                                                                   ccTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGAcc                <  1:350760/151‑1 (MQ=255)                                                                                                                                    cTGACCTTTTTTGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGAcc                >  2:427811/1‑150 (MQ=255)                                                                                                                                            ttttGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTc       >  1:1404484/1‑151 (MQ=255)                                                                                                                                             tttGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTc       <  1:1431005/150‑1 (MQ=255)                                                                                                                                              ttGTCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCt      <  1:216402/150‑1 (MQ=255)                                                                                                                                                 tCCGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGgtg  >  2:283206/1‑151 (MQ=255)                                                                                                                                                  ccGACAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCTCTTTCTTTATACCCCATCTCACCCATGCGATGTTTCTCCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGgtg  <  2:1100097/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTG  >  NC_000913/82292‑82586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑