Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	767,750	C→T	V589V (GTC→GTT)	mngA →	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	767,750	0	C	T	100.0%	313.8 / NA	78	V589V (GTC→GTT)	mngA	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/45);  total (33/45)

TTAAAGAGTTTGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCT  >  NC_000913/767602‑767896                                                                                                                                                     |                                                                                                                                                   ttAAAGAGTTTGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTgg                                                                                                                                                  >  2:830301/1‑151 (MQ=255)    aaGAGTTTGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGc                                                                                                                                               <  1:177076/151‑1 (MQ=255)     agagTTTGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCg                                                                                                                                              >  2:366510/1‑151 (MQ=255)          ttGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGa                                                                                                                                          >  2:1221245/1‑150 (MQ=255)            gAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAat                                                                                                                                        >  1:602460/1‑150 (MQ=255)             aaaTTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAatat                                                                                                                                      <  2:919989/151‑1 (MQ=255)              aaTTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATc                                                                                                                                     <  1:22828/151‑1 (MQ=255)                 tGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCgg                                                                                                                                   >  2:817720/1‑150 (MQ=255)                      ccGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCttt                                                                                                                              <  1:159874/150‑1 (MQ=255)                      ccGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTc                                                                                                                             >  1:161968/1‑151 (MQ=255)                       cGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTct                                                                                                                            <  2:428219/151‑2 (MQ=255)                        gggAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGa                                                                                                                           <  1:1318565/151‑1 (MQ=255)                        gggAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGa                                                                                                                           <  2:825876/151‑1 (MQ=255)                         ggAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGac                                                                                                                          <  2:889217/151‑1 (MQ=255)                                cACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCgg                                                                                                                    >  2:1404677/1‑150 (MQ=255)                                cACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGt                                                                                                                   >  1:268147/1‑151 (MQ=255)                                  ccTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGt                                                                                                                   >  1:398844/1‑149 (MQ=255)                                     ggCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCg                                                                                                               <  1:366559/150‑1 (MQ=255)                                         gTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCa                                                                                                            <  1:636544/149‑1 (MQ=255)                                           tAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCAttt                                                                                                         <  2:807499/150‑1 (MQ=255)                                           tAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCAttt                                                                                                         <  2:1233567/150‑1 (MQ=255)                                           tAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCAttt                                                                                                         <  2:190313/150‑1 (MQ=255)                                            aGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATCGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTtc                                                                                                       <  1:592273/151‑1 (MQ=255)                                             gggCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTtct                                                                                                      <  1:149556/151‑1 (MQ=255)                                                 tGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGct                                                                                                  >  2:1321573/1‑151 (MQ=255)                                                   gCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGctct                                                                                                <  1:1429061/151‑1 (MQ=255)                                                    cAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCtt                                                                                               <  2:781095/151‑1 (MQ=255)                                                    cAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCCCtt                                                                                               >  2:1289303/1‑151 (MQ=255)                                                     aGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCttt                                                                                              >  1:1156468/1‑151 (MQ=255)                                                      ggTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCtttt                                                                                             <  1:1318226/151‑1 (MQ=255)                                                      ggTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCttt                                                                                              <  2:735967/150‑1 (MQ=255)                                                         aTTACCGTAGGGGCTATGCCGAAGGCGATTGAAGATCCGCTGGGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTTTTGTTGGTGCGATGAATATCGGCCTTTCGGCACCCGGTGGCGGCATTTTCTCGCTCTTTTTa                                                                                           <  2:1090755/150‑1 (MQ=255)                                                           tACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACt                                                                                         <  1:348692/150‑1 (MQ=255)                                                            aCCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACtt                                                                                        <  2:318323/150‑1 (MQ=255)                                                             ccGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCa                                                                                      <  1:1188710/151‑1 (MQ=255)                                                               gAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATg                                                                                    >  2:490740/1‑151 (MQ=255)                                                                  ggggCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATa                                                                                 <  1:789612/151‑1 (MQ=255)                                                                  ggggCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATa                                                                                 <  2:872177/151‑1 (MQ=255)                                                                     gCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATg                                                                              <  1:1263891/151‑1 (MQ=255)                                                                       gATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGgc                                                                            >  2:503423/1‑151 (MQ=255)                                                                           ccGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCgcgg                                                                        <  1:1353395/151‑1 (MQ=255)                                                                             gATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCgcgggc                                                                      >  2:897565/1‑151 (MQ=255)                                                                                 gCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGgtg                                                                  <  2:744051/151‑1 (MQ=255)                                                                                    aTTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTCTTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGgtgt                                                                 >  1:552517/1‑149 (MQ=255)                                                                                       gAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATg                                                             >  2:654617/1‑150 (MQ=255)                                                                                        aaGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATggc                                                           <  2:784706/151‑1 (MQ=255)                                                                                         aGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATggcg                                                          <  2:247419/151‑1 (MQ=255)                                                                                             ccGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATggcggc                                                        >  2:733541/1‑149 (MQ=255)                                                                                              cGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAAt                                                     >  2:425922/1‑151 (MQ=255)                                                                                               gCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCCAtt                                                    >  1:1189378/1‑151 (MQ=255)                                                                                               gCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAAtt                                                    <  1:400298/151‑1 (MQ=255)                                                                                               gCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGGGTTATGGCGGCAAtt                                                    >  1:974751/1‑151 (MQ=255)                                                                                                  gCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTgg                                                  <  2:419649/150‑1 (MQ=255)                                                                                                   cGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCt                                                >  1:207046/1‑151 (MQ=255)                                                                                                    gggTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCt                                                >  1:181996/1‑150 (MQ=255)                                                                                                    gggTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGAAGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCCATTGGc                                                 >  2:200616/1‑149 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTTGt                                             >  2:524569/1‑148 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGttt                                           <  1:688919/150‑1 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGttt                                           <  2:199214/150‑1 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGttt                                           <  2:688206/150‑1 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGttt                                           >  2:82278/1‑150 (MQ=255)                                                                                                         aTTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTg                                          <  1:1130327/151‑1 (MQ=255)                                                                                                          ttGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGttt                                           >  2:420970/1‑149 (MQ=255)                                                                                                            ggTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGgcg                                       <  2:1236421/151‑1 (MQ=255)                                                                                                                cGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGGGCgg                                    >  2:1293151/1‑150 (MQ=255)                                                                                                                   ttGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGGAtt                                >  1:1243327/1‑151 (MQ=255)                                                                                                                   ttGTGCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCAt                                 <  1:608314/150‑1 (MQ=255)                                                                                                                      tgCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCCTtgg                              >  2:888611/1‑150 (MQ=255)                                                                                                                       gCTGGGCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggtg                            <  2:1156374/151‑1 (MQ=255)                                                                                                                            gCTCTATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGc                       <  1:1404784/151‑1 (MQ=255)                                                                                                                               ctATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTTGCTGGGTTGGCGCGGCAATgggggggg                        >  1:393589/1‑147 (MQ=255)                                                                                                                               ctATGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCt                      <  2:602389/149‑1 (MQ=255)                                                                                                                                 aTGGTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTgga                   >  2:146933/1‑148 (MQ=255)                                                                                                                                   ggTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAt                 <  2:99492/150‑1 (MQ=255)                                                                                                                                    gTAACGGGCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAtct               <  2:361499/151‑1 (MQ=255)                                                                                                                                          ggCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTg         <  1:830381/151‑1 (MQ=255)                                                                                                                                          ggCGCTATTGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTg         <  1:1211436/151‑1 (MQ=255)                                                                                                                                                  tGTTGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATtct  >  1:1228944/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTAAAGAGTTTGAAATTGAGACCGGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCT  >  NC_000913/767602‑767896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑