Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	939,609	C→T	A61V (GCG→GTG)	serS →	seryl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	939,609	0	C	T	98.7%	284.4 / ‑3.9	77	A61V (GCG→GTG)	serS	seryl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (37/38);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

ACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGCGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATG  >  NC_000913/939462‑939755                                                                                                                                                    |                                                                                                                                                   aCGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGaa                                                                                                                                                 <  1:1238684/151‑1 (MQ=255)  cGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGaaa                                                                                                                                                <  1:1373678/151‑1 (MQ=255)   gCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGaaa                                                                                                                                                <  2:402682/150‑1 (MQ=255)   gCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGaa                                                                                                                                                 >  1:1225027/1‑149 (MQ=255)   gCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGaa                                                                                                                                                 >  1:629469/1‑149 (MQ=255)    cAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAgc                                                                                                                                              >  2:1049247/1‑151 (MQ=255)        cGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAgcgcgc                                                                                                                                          >  2:1443443/1‑151 (MQ=255)        cGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAgcgcg                                                                                                                                           <  1:576531/150‑1 (MQ=255)        cGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAgcgcg                                                                                                                                           <  1:395877/150‑1 (MQ=255)         gCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGcgcgcg                                                                                                                                         >  1:359876/1‑151 (MQ=255)            gAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCgg                                                                                                                                        <  2:1186756/149‑1 (MQ=255)             aaaaaCTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCgggg                                                                                                                                      <  1:569297/150‑1 (MQ=255)                 aCTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCgggg                                                                                                                                      >  2:658944/1‑146 (MQ=255)                    ggCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATc                                                                                                                              >  1:1107431/1‑151 (MQ=255)                      cACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCg                                                                                                                             >  1:824379/1‑150 (MQ=255)                      cACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGa                                                                                                                            <  1:882493/151‑1 (MQ=255)                          ccGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCt                                                                                                                        <  2:1450463/151‑1 (MQ=255)                           cGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCtt                                                                                                                       >  2:447074/1‑151 (MQ=255)                            ggggCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCttt                                                                                                                      <  1:110100/151‑1 (MQ=255)                             gggCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTa                                                                                                                     <  1:1176986/151‑1 (MQ=255)                              ggCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTAc                                                                                                                    <  1:752098/151‑1 (MQ=255)                              ggCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTAc                                                                                                                    >  1:1077549/1‑151 (MQ=255)                                cTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGt                                                                                                                  >  1:680492/1‑151 (MQ=255)                                  ttAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCt                                                                                                                >  2:58384/1‑151 (MQ=255)                                   tAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCt                                                                                                                <  1:442638/150‑1 (MQ=255)                                    aaGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTgg                                                                                                              >  2:966520/1‑151 (MQ=255)                                       cTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGa                                                                                                             >  2:997342/1‑149 (MQ=255)                                          gATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGa                                                                                                        <  2:1222426/151‑1 (MQ=255)                                          gATGGAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGAATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCAAGGTGAAAGCGCGCGGGGAAGATATCGACCCTTTACGTCTGGAAggg                                                                                                         >  2:1243444/1‑148 (MQ=255)                                               aGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACaa                                                                                                    >  1:600417/1‑150 (MQ=255)                                                   aaGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTgg                                                                                               <  2:858353/151‑1 (MQ=255)                                                    aGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGCGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTggg                                                                                              >  2:252036/1‑151 (MQ=255)                                                     gCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGc                                                                                             <  2:603600/151‑1 (MQ=255)                                                       tGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCg                                                                                            >  1:341146/1‑150 (MQ=255)                                                        gggCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCgt                                                                                           <  1:997110/150‑2 (MQ=255)                                                        gggCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGaa                                                                                          <  1:1177984/151‑1 (MQ=255)                                                         ggCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGa                                                                                           >  1:273832/1‑149 (MQ=255)                                                            gcTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAagaga                                                                                      <  1:313219/151‑2 (MQ=255)                                                              tctTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTg                                                                                    >  2:214611/1‑151 (MQ=255)                                                              tctTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTg                                                                                    >  1:1052044/1‑151 (MQ=255)                                                                 tGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGa                                                                                  <  1:961396/150‑1 (MQ=255)                                                                   aaGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATgc                                                                               >  1:855313/1‑151 (MQ=255)                                                                    agagCGTCGTAAAGTATTACAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATgc                                                                               >  1:691353/1‑150 (MQ=255)                                                                              aaaGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGcc                                                                     <  2:583399/150‑1 (MQ=255)                                                                                  tATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCt                                                                >  1:1394689/1‑151 (MQ=255)                                                                                   aTTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTg                                                               >  2:674405/1‑151 (MQ=255)                                                                                    ttGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGCGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTgg                                                              >  2:22558/1‑151 (MQ=255)                                                                                      gCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGAt                                                            <  2:554439/151‑1 (MQ=255)                                                                                                aCGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAgg                                                  <  2:305643/151‑1 (MQ=255)                                                                                                 cGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGc                                                 <  2:934428/151‑1 (MQ=255)                                                                                                     aaaCCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGaa                                             <  2:1307976/151‑1 (MQ=255)                                                                                                     aaaCCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGa                                              >  1:112872/1‑150 (MQ=255)                                                                                                       aCCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAAt                                           >  2:1324955/1‑151 (MQ=255)                                                                                                       aCCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAAt                                           <  1:1049343/151‑1 (MQ=255)                                                                                                        ccTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAAtt                                          <  2:358133/151‑1 (MQ=255)                                                                                                         cTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTc                                         >  2:1372732/1‑151 (MQ=255)                                                                                                           gCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTcgc                                       <  1:1363238/151‑1 (MQ=255)                                                                                                           gCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTc                                         <  1:962373/149‑1 (MQ=255)                                                                                                            cAAGCGGAGCGTATCTCCCAAGCGAAATCCATTGGCCAGGGGGAAGCGCGCGGGGAAGATATCGAGCTTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTagcg                                      >  2:131713/1‑151 (MQ=255)                                                                                                            cAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTcgcg                                      <  1:781003/151‑1 (MQ=255)                                                                                                             aaGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTcgcg                                      >  1:591221/1‑150 (MQ=255)                                                                                                             aaGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTcgcg                                      >  1:937193/1‑150 (MQ=255)                                                                                                             aaGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGa                                     <  2:1282971/151‑1 (MQ=255)                                                                                                             aaGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGa                                     <  2:1098625/151‑1 (MQ=255)                                                                                                               gCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGat                                    >  1:425937/1‑150 (MQ=255)                                                                                                                 ggAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATc                                 <  1:58393/151‑1 (MQ=255)                                                                                                                 ggAGCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATc                                 <  1:1270055/151‑1 (MQ=255)                                                                                                                    gCGTAACTCCCGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATcgcg                              >  1:973032/1‑151 (MQ=255)                                                                                                                              cGATCGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATcc                    >  1:618642/1‑151 (MQ=255)                                                                                                                                  cGAAATCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTa                 <  2:1051949/150‑1 (MQ=255)                                                                                                                                      aTCCATTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAAcctg            >  1:57009/1‑151 (MQ=255)                                                                                                                                          aTTGGCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAAcctgcctg        >  1:479589/1‑151 (MQ=255)                                                                                                                                             ggCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCa      <  1:200193/150‑1 (MQ=255)                                                                                                                                             ggCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCa      <  2:876772/150‑1 (MQ=255)                                                                                                                                             ggCCAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAg     <  2:680418/151‑1 (MQ=255)                                                                                                                                                cAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATg  >  2:712971/1‑151 (MQ=255)                                                                                                                                                cAGGTGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATg  >  2:26189/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGCGCTCTTGAAGAGCGTCGTAAAGTATTGCAGGTCAAAACGGAAAACCTGCAAGCGGAGCGTAACTCCCGATCGAAATCCATTGGCCAGGCGAAAGCGCGCGGGGAAGATATCGAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATG  >  NC_000913/939462‑939755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑