Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,630	A→G	K89E (AAG→GAG)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,630	0	A	G	98.9%	341.6 / ‑4.0	93	K89E (AAG→GAG)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (45/47);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CGCCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAAC  >  NC_000913/968485‑968777                                                                                                                                                  |                                                                                                                                                    cgcCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCt                                                                                                                                                >  1:921627/1‑151 (MQ=255)  gcCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTg                                                                                                                                               <  2:1223743/151‑1 (MQ=255)  gcCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTg                                                                                                                                               <  1:36403/151‑1 (MQ=255)  gcCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTg                                                                                                                                               >  2:385363/1‑151 (MQ=255)    cTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGa                                                                                                                                              >  1:1045085/1‑150 (MQ=255)      ggCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGaa                                                                                                                                             <  2:829255/149‑1 (MQ=255)        cGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCtt                                                                                                                                         <  1:354301/151‑1 (MQ=255)         gTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCtt                                                                                                                                         <  2:507696/150‑1 (MQ=255)           gCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTAt                                                                                                                                       <  1:692559/150‑1 (MQ=255)           gCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATc                                                                                                                                      <  1:1314419/151‑1 (MQ=255)             ccccGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCg                                                                                                                                    <  1:628524/151‑1 (MQ=255)             ccccGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCg                                                                                                                                    >  1:35577/1‑151 (MQ=255)             ccccGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCg                                                                                                                                    >  2:532957/1‑151 (MQ=255)               ccGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCt                                                                                                                                  >  2:1369673/1‑151 (MQ=255)                cGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTg                                                                                                                                 <  2:360787/151‑1 (MQ=255)                cGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTg                                                                                                                                 >  1:1146230/1‑151 (MQ=255)                cGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTg                                                                                                                                 >  2:1031617/1‑151 (MQ=255)                cGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTg                                                                                                                                 <  1:50016/151‑1 (MQ=255)                 gTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTg                                                                                                                                 <  2:4380/150‑1 (MQ=255)                  tACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGAAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGtt                                                                                                                               >  1:1013900/1‑151 (MQ=255)                   aCCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGtt                                                                                                                               <  2:177376/150‑1 (MQ=255)                   aCCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGtt                                                                                                                               <  1:1057102/150‑1 (MQ=255)                      ggTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTg                                                                                                                           >  2:1078444/1‑151 (MQ=255)                         tGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCg                                                                                                                        >  1:36394/1‑151 (MQ=255)                          gTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCgg                                                                                                                       >  2:172138/1‑151 (MQ=255)                           tCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCg                                                                                                                        <  1:731984/149‑1 (MQ=255)                            cGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGc                                                                                                                      <  2:877660/150‑1 (MQ=255)                              tggttggtAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGc                                                                                                                      >  1:365575/1‑148 (MQ=255)                              tggttggtAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGc                                                                                                                      >  2:452041/1‑148 (MQ=255)                               ggttggtAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGAt                                                                                                                  <  2:296594/151‑1 (MQ=255)                                gttggtAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATa                                                                                                                 <  1:76645/151‑1 (MQ=255)                                 ttggtAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATAc                                                                                                                >  1:677744/1‑151 (MQ=255)                                     tAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACcac                                                                                                             >  2:1068070/1‑150 (MQ=255)                                      aaTCTCAACGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACcaca                                                                                                            <  1:531872/150‑1 (MQ=255)                                       aTCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCacaac                                                                                                          >  2:824371/1‑151 (MQ=255)                                       aTCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCacaac                                                                                                          >  2:118382/1‑151 (MQ=255)                                           cACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCacaaca                                                                                                         <  2:1022633/148‑1 (MQ=255)                                           cACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCacaac                                                                                                          <  1:1024901/147‑1 (MQ=255)                                           cACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGc                                                                                                       >  2:289617/1‑150 (MQ=255)                                            aCCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGcac                                                                                                     >  2:1392602/1‑151 (MQ=255)                                              cGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAg                                                                                                   <  1:138019/151‑1 (MQ=255)                                               gCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAgg                                                                                                  >  1:439942/1‑151 (MQ=255)                                                    cggcAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCgggg                                                                                             >  1:275331/1‑150 (MQ=255)                                                     ggcAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCgg                                                                                               >  1:1131065/1‑148 (MQ=255)                                                         aCGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGtgatga                                                                                        <  2:183075/151‑1 (MQ=255)                                                          cGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGaa                                                                                       <  1:878026/151‑1 (MQ=255)                                                           ggAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGgtggtgga                                                                                       >  2:281375/1‑148 (MQ=255)                                                             aaaaaCCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAcc                                                                                     >  2:727609/1‑150 (MQ=255)                                                             aaaaaCCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCt                                                                                    <  1:1142790/151‑1 (MQ=255)                                                                  ccccGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGtt                                                                               <  1:357704/151‑1 (MQ=255)                                                                    ccGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGtt                                                                               >  1:1005491/1‑149 (MQ=255)                                                                     cGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGAt                                                                            >  2:618145/1‑151 (MQ=255)                                                                     cGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGAt                                                                            <  1:394041/151‑1 (MQ=255)                                                                     cGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGAt                                                                            <  1:1369777/151‑1 (MQ=255)                                                                     cGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGGTGAACCTGTGtt                                                                               >  2:647404/1‑148 (MQ=255)                                                                         cGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGGTGAACCCGTGTTGATTTAt                                                                        >  2:904477/1‑151 (MQ=255)                                                                         cGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTAt                                                                        <  2:1378317/151‑1 (MQ=255)                                                                            tGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCa                                                                      <  2:1050472/150‑1 (MQ=255)                                                                             gTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCaa                                                                     <  2:1199079/150‑1 (MQ=255)                                                                              tCTGGCTGGTGGAACAGTTGCAACAGCGCGGGATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAAACTGTGTTGGTTTTTCaa                                                                     >  2:596569/1‑149 (MQ=255)                                                                                 ggctggTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCAc                                                                <  2:544790/151‑1 (MQ=255)                                                                                  gctggTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGATGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGTTTAGCCATTGTTGATTTATTaaatgac                                                                >  2:846996/1‑145 (MQ=255)                                                                                    tggtggAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGGTGAACCCGTGGTGGttttttaaaccac                                                                >  2:964295/1‑138 (MQ=255)                                                                                      gtggAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGAt                                                            >  1:193828/1‑150 (MQ=255)                                                                                        ggAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATgcg                                                         <  1:798858/151‑1 (MQ=255)                                                                                        ggAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATgc                                                          <  1:43465/150‑1 (MQ=255)                                                                                         gAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATgcgc                                                        <  2:426405/151‑1 (MQ=255)                                                                                          aaCAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCGcc                                                       >  1:146850/1‑151 (MQ=255)                                                                                            cAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCCGTGTTGATTTTTCAACGCACTGGTGCGcc                                                       >  1:1350295/1‑149 (MQ=255)                                                                                              gTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAACTGTGTTGATTTATCAACGCACTGATGCGCCTGtt                                                   >  2:1306543/1‑151 (MQ=255)                                                                                                 gCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCg                                                <  1:118399/151‑1 (MQ=255)                                                                                                     cAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGTTTTATCAAACCACAGATGCGCCCGTTGCGGgtt                                            >  2:895134/1‑151 (MQ=255)                                                                                                     cAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGGTTTATCAACGCACTGATGGGCCCGTTGCGGttt                                            >  2:1437650/1‑151 (MQ=255)                                                                                                     cAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGttt                                            <  2:590128/151‑1 (MQ=255)                                                                                                      aGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTtc                                           <  2:1356466/151‑1 (MQ=255)                                                                                                           ggTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcg                                      <  2:99813/151‑1 (MQ=255)                                                                                                           ggTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcg                                      <  1:1031716/151‑1 (MQ=255)                                                                                                              aTTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGGAACCGTGTTGGTTTATTAACGCACTGATGCGCCTGTTGCGGGTTTTCCcctt                                    >  2:223008/1‑150 (MQ=255)                                                                                                              aTTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcgtt                                    >  1:781914/1‑150 (MQ=255)                                                                                                              aTTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcgttc                                   <  1:1200393/151‑1 (MQ=255)                                                                                                                tCGCCTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcgttcg                                  <  1:441060/150‑1 (MQ=255)                                                                                                                 cgcgTGGGGGTCGTATCGCGGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCcgttcgt                                 <  2:1044989/150‑1 (MQ=255)                                                                                                                        gggTAGTATCGCGGGTATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATTCCACAACAGCACTGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGc                         <  2:523191/151‑1 (MQ=255)                                                                                                                                 cgcgGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAg                 <  2:1130967/150‑1 (MQ=255)                                                                                                                                   cgGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAACTGTGTTGATTTATCAACGCACTGATGCGCCCGTTGCGGGTTTTTCCGTTTGTTTTGATGGGGGAAAAAcc               >  2:1225007/1‑150 (MQ=255)                                                                                                                                   cgGGGATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCCGTTGCGGGTTTTCCCCTTCGTTCTGATTCGGGAAAAAcc               >  1:1424521/1‑150 (MQ=255)                                                                                                                                    ggggATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCAt             <  2:545974/151‑1 (MQ=255)                                                                                                                                    ggggATATGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCAt             <  2:921540/151‑1 (MQ=255)                                                                                                                                        atatGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGAAGAACCTGTGTTGGTTTATCAACGCACTGATGCGCCTGTTGCGGGTTCTCCCGTTCGTTCTGATGCGGGAAAAGCCCtt            >  2:1331888/1‑148 (MQ=255)                                                                                                                                        atatGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTTTCAACGCACTGATGCGCCTGGTTCGGTTTTTCCCGTTTGTTTTGATGCGGGAAAAACCAtt            >  2:827232/1‑148 (MQ=255)                                                                                                                                          atGGTGGTGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAACTGTGTTGATTTATCAACCCACTGATGCGCCCGTTGCGGGTTCTCCCGGTCGTTCTGATGCGGGAAAAACCATTTTgg        >  2:1234415/1‑150 (MQ=255)                                                                                                                                            ggtggtGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGgcg      <  1:385414/150‑1 (MQ=255)                                                                                                                                               ggtGAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAac  <  1:172160/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CGCCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGAAAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAAC  >  NC_000913/968485‑968777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑