Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,782	A→G	E224G (GAA→GGA)	yceG →	septation protein, ampicillin sensitivity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,782	0	A	G	100.0%	360.7 / NA	94	E224G (GAA→GGA)	yceG	septation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/40);  total (54/40)

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924                                                                                                                                                     |                                                                                                                                               tCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcg                                                                                                                                             >  1:1137630/1‑151 (MQ=255)   gcgTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcgcg                                                                                                                                           >  2:944071/1‑151 (MQ=255)    cgTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcgcg                                                                                                                                           >  1:726458/1‑150 (MQ=255)         cTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATa                                                                                                                                        <  1:554225/148‑1 (MQ=255)         cTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAgg                                                                                                                                     >  2:1272540/1‑151 (MQ=255)            aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTg                                                                                                                                  <  1:330038/151‑1 (MQ=255)             agcgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGc                                                                                                                                 >  1:825610/1‑151 (MQ=255)                  gggcAAAAAAAAAGGGTGAAAGCGGTGGTTAGCCCTTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAACCCAGTTGGTGACGATGGCATCATTTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCa                                                                                                                             <  1:738066/148‑1 (MQ=255)                  gcgcACAAGAAAATGGTGAAAGCGGTCGATAGCCCCTGGGAAGGGCGGGCGGCCGGTCTGCCTTAAAAA‑AAAAAAAACCAGTTGGTGACGAGGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAg                                                                                                                            <  2:227270/151‑1 (MQ=255)                  gcgcACAAAAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGAGGGCATAAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTc                                                                                                                              <  2:47047/149‑1 (MQ=255)                    gcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGt                                                                                                                           >  2:403637/1‑150 (MQ=255)                    gcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGt                                                                                                                           >  1:74955/1‑150 (MQ=255)                     caaaaGAAAAGGGTAAAAGCGGTCGATAGCGCCGGGGAAGGGCGTGCGGACGGTCTGCCTTAAAAA‑GATAAAAACCAGTTGGGGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAg                                                                                                                            <  2:1123815/145‑1 (MQ=255)                      acaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAACCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                         <  1:779001/150‑1 (MQ=255)                      acaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTa                                                                                                                          <  2:228522/149‑1 (MQ=255)                      acaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGGGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGCCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                        <  1:1413666/151‑1 (MQ=255)                                    aaggCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTAc                                                                                                          <  1:1222791/148‑1 (MQ=255)                                    aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTTTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGAGGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTAc                                                                                                          <  1:961191/151‑1 (MQ=255)                                    aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTAc                                                                                                          <  2:1253402/151‑1 (MQ=255)                                     aaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACg                                                                                                         >  1:1296027/1‑151 (MQ=255)                                      aGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGc                                                                                                        >  1:927937/1‑151 (MQ=255)                                       gCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCa                                                                                                       <  1:823863/151‑1 (MQ=255)                                        cGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCa                                                                                                       >  1:186288/1‑150 (MQ=255)                                         ggTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAtt                                                                                                     >  2:1406673/1‑151 (MQ=255)                                         ggTCGATAGCGCCTGGGAAGGGCGGGCGGACGGTCTGCCTTAAAAA‑GAAAAAAACCGGTTGGGGACGAGGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAtt                                                                                                     <  1:770663/151‑1 (MQ=255)                                             gATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGt                                                                                                  >  1:916450/1‑150 (MQ=255)                                                aGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATgc                                                                                              >  2:53028/1‑151 (MQ=255)                                                  cgcTTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑AATAAAAACCAGTTGGTGACGATGGCTTCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTg                                                                                                    <  1:702912/143‑1 (MQ=255)                                                     cTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         >  2:612597/1‑151 (MQ=255)                                                     cTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         >  1:70608/1‑151 (MQ=255)                                                       gggAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGct                                                                                       <  1:430605/151‑2 (MQ=255)                                                            gggCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgacc                                                                                   <  1:944160/150‑1 (MQ=255)                                                             ggCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  >  1:20431/1‑150 (MQ=255)                                                                gTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgaccc                                                                              >  2:625974/1‑151 (MQ=255)                                                                  gCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAAAccgacccg                                                                             >  1:165548/1‑150 (MQ=255)                                                                   cGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccgac                                                                           >  2:69243/1‑151 (MQ=255)                                                                    ggACGGTCTGCCTTATAAA‑GATAAAACCCAGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          <  2:6242/151‑1 (MQ=255)                                                                    ggAAGGTCTG‑CTTATAAAGGATAAAAACAGGTGGGGGACTATGGCAGCAATTATGGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  <  2:1423911/143‑1 (MQ=255)                                                                     gccggtttcctttAAAAA‑AAAAAAAACCAGTTGGGGGCGATGGCTTCAATTACCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgaccca                                                                             <  2:1288877/136‑2 (MQ=255)                                                                      aCGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGt                                                                        >  1:220665/1‑151 (MQ=255)                                                                      aCGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGt                                                                        >  1:319847/1‑151 (MQ=255)                                                                             gCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 >  2:370961/1‑151 (MQ=255)                                                                             gCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 >  1:525910/1‑151 (MQ=255)                                                                              ccTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACg                                                                >  1:906696/1‑151 (MQ=255)                                                                               cTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                               >  1:192794/1‑151 (MQ=255)                                                                                 tataAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACggg                                                              >  2:277312/1‑150 (MQ=255)                                                                                  aaaaa‑GAAAAAAACCAGTGGGGGACGAGGGCATCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGAt                                                            <  1:594529/149‑1 (MQ=255)                                                                                   taAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           >  2:748833/1‑151 (MQ=255)                                                                                    aaa‑TATAAAACCCAGTGGGTGACGATGCCATAAATTACCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACggg                                                              <  2:941694/147‑1 (MQ=255)                                                                                    aaa‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATgg                                                          <  1:830562/151‑1 (MQ=255)                                                                                    aaa‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATgg                                                          >  1:1049058/1‑151 (MQ=255)                                                                                           aaaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                    >  1:712831/1‑151 (MQ=255)                                                                                            aaacCCAGTTGGTGACGATGGCATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCg                                                   <  2:779320/151‑1 (MQ=255)                                                                                            aaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCg                                                   >  1:1246624/1‑151 (MQ=255)                                                                                             aaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGt                                                  >  2:750381/1‑151 (MQ=255)                                                                                                      ggTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGc                                         >  2:1061243/1‑151 (MQ=255)                                                                                                       gTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGc                                         >  1:784464/1‑150 (MQ=255)                                                                                                           cGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    >  2:359168/1‑151 (MQ=255)                                                                                                            gATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACtt                                   >  1:744795/1‑151 (MQ=255)                                                                                                            gATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    <  1:707073/150‑1 (MQ=255)                                                                                                             aTGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                  >  1:634594/1‑151 (MQ=255)                                                                                                             aTGGCATCAATTACCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                       <  2:1035082/146‑1 (MQ=255)                                                                                                               ggCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                  <  2:1285639/149‑1 (MQ=255)                                                                                                               ggCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtc                                 <  1:1228258/150‑1 (MQ=255)                                                                                                                 cATCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtctc                               <  1:784738/150‑1 (MQ=255)                                                                                                                     aaTTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             <  2:74947/148‑1 (MQ=255)                                                                                                                      aTTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                           >  1:321968/1‑149 (MQ=255)                                                                                                                        tATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                           >  2:1215921/1‑147 (MQ=255)                                                                                                                        tATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGa                        <  1:859789/150‑1 (MQ=255)                                                                                                                         aTCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAcc                      >  1:730008/1‑151 (MQ=255)                                                                                                                         aTCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                       <  1:1463658/150‑1 (MQ=255)                                                                                                                         aTCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                           <  1:1216020/146‑1 (MQ=255)                                                                                                                          tCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAACCTTTCTCGTGCAGAcc                      <  1:847994/150‑1 (MQ=255)                                                                                                                          tCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCt                     >  1:177287/1‑151 (MQ=255)                                                                                                                           cGAAAAAGAAGCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGAATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGCCCGACCCGCCCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                    >  2:746859/1‑151 (MQ=255)                                                                                                                           cGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                    >  2:390395/1‑151 (MQ=255)                                                                                                                            gAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                   >  1:680854/1‑151 (MQ=255)                                                                                                                             aaaaaaaaACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGa                  <  2:916363/151‑1 (MQ=255)                                                                                                                             aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCt                     <  1:352454/148‑1 (MQ=255)                                                                                                                                 aGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACg              >  1:948479/1‑151 (MQ=255)                                                                                                                                  gAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGc             >  2:981682/1‑151 (MQ=255)                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            >  1:1440242/1‑151 (MQ=255)                                                                                                                                    aaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg           <  2:726378/151‑1 (MQ=255)                                                                                                                                     aCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg           >  2:925302/1‑150 (MQ=255)                                                                                                                                      ccgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  1:505707/1‑151 (MQ=255)                                                                                                                                       cgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  2:284979/1‑150 (MQ=255)                                                                                                                                        gccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  2:1366360/1‑149 (MQ=255)                                                                                                                                        gccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  1:1243296/1‑149 (MQ=255)                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      <  1:1319634/151‑1 (MQ=255)                                                                                                                                           gTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt    >  1:295187/1‑151 (MQ=255)                                                                                                                                            ttGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt    >  2:1066564/1‑150 (MQ=255)                                                                                                                                            ttGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt    >  1:60436/1‑150 (MQ=255)                                                                                                                                              gCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGtat  <  2:634519/150‑1 (MQ=255)                                                                                                                                              gCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGtat  <  1:110796/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAA‑GATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑