Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,293,491 C→T intergenic (‑569/‑36) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,293,4910CT99.0% 382.9 / NA 99intergenic (‑569/‑36)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (48/50);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAGTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGCCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGTATATACGG  >  NC_000913/1293344‑1293638
                                                                                                                                                   |                                                                                                                                                   
cagcagTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCaa                                                                                                                                                  <  1:1234284/151‑1 (MQ=255)
cagcagTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCaa                                                                                                                                                  <  1:1299168/151‑1 (MQ=255)
  gcagTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACt                                                                                                                                                <  2:881877/151‑1 (MQ=255)
   cagTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACtt                                                                                                                                               >  2:537939/1‑151 (MQ=255)
      tCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTAt                                                                                                                                            >  1:183990/1‑151 (MQ=255)
       cATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTAt                                                                                                                                            <  2:1341698/150‑1 (MQ=255)
         tttACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTAttt                                                                                                                                          >  2:1383757/1‑150 (MQ=255)
          ttACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTc                                                                                                                                         <  2:503074/150‑1 (MQ=255)
            aCCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCata                                                                                                                                      <  2:38231/151‑1 (MQ=255)
            aCCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCata                                                                                                                                      <  1:530419/151‑1 (MQ=255)
            aCCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCata                                                                                                                                      <  1:1024314/151‑1 (MQ=255)
             cccGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCatat                                                                                                                                     >  1:856384/1‑151 (MQ=255)
              ccGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATc                                                                                                                                    <  2:725365/151‑1 (MQ=255)
                gCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCAt                                                                                                                                  <  1:205671/151‑1 (MQ=255)
                   tataACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTg                                                                                                                                >  2:58872/1‑150 (MQ=255)
                   tataACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTg                                                                                                                                <  1:163320/150‑1 (MQ=255)
                   tataACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTg                                                                                                                                <  2:1308993/150‑1 (MQ=255)
                   tataACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTg                                                                                                                                <  2:301528/150‑1 (MQ=255)
                      aaCAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAggg                                                                                                                            >  2:133698/1‑151 (MQ=255)
                        cAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGcc                                                                                                                          <  1:328831/151‑1 (MQ=255)
                        cAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGcc                                                                                                                          >  2:543073/1‑151 (MQ=255)
                         aaGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCt                                                                                                                         >  1:396344/1‑151 (MQ=255)
                           gCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCtgt                                                                                                                       <  1:485311/151‑1 (MQ=255)
                           gCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCtgt                                                                                                                       >  2:47950/1‑151 (MQ=255)
                                   gTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATg                                                                                                               <  1:253059/151‑1 (MQ=255)
                                       tAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGcac                                                                                                           >  2:248468/1‑151 (MQ=255)
                                        aaTGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGcaca                                                                                                          <  1:1319533/151‑1 (MQ=255)
                                        aaTGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGcac                                                                                                           <  1:724931/150‑1 (MQ=255)
                                           gATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCt                                                                                                       <  1:105802/151‑1 (MQ=255)
                                            aTAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCta                                                                                                      <  2:41592/151‑1 (MQ=255)
                                                  cAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATAttt                                                                                                 <  2:263518/150‑1 (MQ=255)
                                                   aGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCCTATACACACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGATGATGGCAAAGCTATATTTc                                                                                                >  2:401477/1‑150 (MQ=255)
                                                   aGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTct                                                                                               >  2:576246/1‑151 (MQ=255)
                                                   aGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTct                                                                                               >  2:391551/1‑151 (MQ=255)
                                                       ggATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTctacta                                                                                           <  1:449286/151‑1 (MQ=255)
                                                       ggATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTctacta                                                                                           <  2:830732/151‑1 (MQ=255)
                                                        gATTATGCAAGGCTTCGAAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTAt                                                                                          >  2:185335/1‑151 (MQ=255)
                                                          ttATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACCGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTc                                                                                        >  2:1000471/1‑151 (MQ=255)
                                                           tATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTc                                                                                        <  1:382874/150‑1 (MQ=255)
                                                            aTGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCg                                                                                      >  1:431627/1‑151 (MQ=255)
                                                            aTGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCg                                                                                      >  2:821127/1‑151 (MQ=255)
                                                               cAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCa                                                                                    >  1:348646/1‑150 (MQ=255)
                                                                aaGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCaat                                                                                  <  1:471773/151‑1 (MQ=255)
                                                                 aGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCaatg                                                                                 >  1:557270/1‑151 (MQ=255)
                                                                       cGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCg                                                                           <  1:550558/151‑1 (MQ=255)
                                                                        gTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCgg                                                                          <  1:1208174/151‑1 (MQ=255)
                                                                        gTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCgg                                                                          >  2:1142178/1‑151 (MQ=255)
                                                                        gTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCg                                                                           <  2:1211015/150‑1 (MQ=255)
                                                                             ggAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTa                                                                       >  1:1022803/1‑149 (MQ=255)
                                                                             ggAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAg                                                                     <  1:660802/151‑1 (MQ=255)
                                                                                 aaCGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTa                                                                 <  2:396290/151‑1 (MQ=255)
                                                                                    gCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAg                                                              <  1:538001/151‑1 (MQ=255)
                                                                                      atatACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGc                                                             >  1:1043998/1‑150 (MQ=255)
                                                                                      atatACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCa                                                            <  1:725665/151‑1 (MQ=255)
                                                                                        ataCCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAtt                                                          >  2:1052755/1‑151 (MQ=255)
                                                                                        ataCCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAtt                                                          <  1:676513/151‑1 (MQ=255)
                                                                                        ataCCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAtt                                                          <  2:1285477/151‑1 (MQ=255)
                                                                                           cccACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAttgtt                                                       >  2:452311/1‑151 (MQ=255)
                                                                                           cccACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAttgtt                                                       <  2:1141200/151‑1 (MQ=255)
                                                                                           cccACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCAttgtt                                                       >  2:572639/1‑151 (MQ=255)
                                                                                              aCTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGc                                                     >  2:1089874/1‑150 (MQ=255)
                                                                                               cTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCa                                                    >  2:61736/1‑150 (MQ=255)
                                                                                                ttCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAAt                                                  >  2:1393534/1‑151 (MQ=255)
                                                                                                    gtgCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTc                                              >  1:808865/1‑151 (MQ=255)
                                                                                                      gCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCa                                             <  1:733348/150‑1 (MQ=255)
                                                                                                      gCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCAt                                            <  2:1090294/151‑1 (MQ=255)
                                                                                                      gCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCAt                                            >  1:726693/1‑151 (MQ=255)
                                                                                                         tACTGTTGAGCTGAAAACCTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATAc                                          >  1:553613/1‑150 (MQ=255)
                                                                                                          aCTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACa                                         <  2:996187/150‑1 (MQ=255)
                                                                                                          aCTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACa                                         <  1:859327/150‑1 (MQ=255)
                                                                                                           cTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAAt                                       >  1:192906/1‑151 (MQ=255)
                                                                                                            tGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAAtt                                      >  1:1243362/1‑151 (MQ=255)
                                                                                                             gTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTa                                     <  2:1464575/151‑1 (MQ=255)
                                                                                                             gTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTa                                     <  1:1451636/151‑1 (MQ=255)
                                                                                                               tGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTAcc                                   >  2:1294263/1‑151 (MQ=255)
                                                                                                               tGAGCTGAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTAc                                    <  2:1434163/150‑1 (MQ=255)
                                                                                                                     gAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAAc                             <  2:820539/151‑1 (MQ=255)
                                                                                                                     gAAAAACTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAAc                             <  2:1163527/151‑1 (MQ=255)
                                                                                                                       gaaaCTGACGACTTATGATAAAGTACAGCCAGCTTTGTTTCATATGATTCTGGGCCGGTGCCTGATGACACAGCAATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCACACAATTACCAg                                 >  2:71667/2‑145 (MQ=255)
                                                                                                                        aaaCTGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAAcgcg                          <  1:394556/151‑1 (MQ=255)
                                                                                                                            tGACGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCAt                      >  1:1375018/1‑151 (MQ=255)
                                                                                                                               cGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATgcg                   >  1:329932/1‑151 (MQ=255)
                                                                                                                               cGAATTATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATgcg                   <  2:697850/151‑1 (MQ=255)
                                                                                                                                    tATGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACt               <  2:905840/150‑1 (MQ=255)
                                                                                                                                     aTGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGt             >  2:1401859/1‑151 (MQ=255)
                                                                                                                                     aTGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGt             >  1:640706/1‑151 (MQ=255)
                                                                                                                                     aTGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGt             >  2:288085/1‑151 (MQ=255)
                                                                                                                                     aTGATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGt             >  2:1469329/1‑151 (MQ=255)
                                                                                                                                       gATAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCg           >  1:356876/1‑151 (MQ=255)
                                                                                                                                        aTAACCTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCACGAACGCGGCATGCGCACTGTCGt          <  2:284195/151‑1 (MQ=255)
                                                                                                                                        aTAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTACGTCTACAGCATAGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGACg           >  2:1025272/1‑150 (MQ=255)
                                                                                                                                        aTAAACTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGt          >  1:789586/1‑151 (MQ=255)
                                                                                                                                          aaaCTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGtat        >  1:183537/1‑151 (MQ=255)
                                                                                                                                            aCTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGtatat      <  1:1078392/151‑1 (MQ=255)
                                                                                                                                            aCTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGtatat      >  1:473914/1‑151 (MQ=255)
                                                                                                                                             cTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGtatat      >  2:663013/1‑150 (MQ=255)
                                                                                                                                             cTCCAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGtatat      >  1:865196/1‑150 (MQ=255)
                                                                                                                                               ccAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGTATATACg   >  1:798969/1‑151 (MQ=255)
                                                                                                                                                cAGTCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGTATATACgg  <  1:1383862/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGCAGTCATTTACCCGCTTATAACAAGCGAGGCAGTTGTAATGATAGCTCAGAAGGATTATGCAAGGCTTCGTAAGGGAGAACGCATATACCCACTTCTGTGCATACTGTTGAGCTGAAAAACTGACGAATTATGATAAACTCCAGCCAACTTTATTTCATATCATTGAGGGCCTGTGGCTGATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACAGCATTGTTGCAATCTTCATACAATTACCAGGAACGCGGCATGCGCACTGTCGTATATACGG  >  NC_000913/1293344‑1293638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: