Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 155,698 A→G G168G (GGT→GGC yadV ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913155,6980AG99.2% 453.9 / ‑3.9 119G168G (GGT→GGCyadVputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (50/68);  total (51/68)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 119 total aligned reads displayed.

CATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGT  >  NC_000913/155550‑155842
                                                                                                                                                    |                                                                                                                                                
cATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGcc                                                                                                                                                >  2:352785/1‑151 (MQ=255)
 aTTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGcc                                                                                                                                                <  2:106469/150‑1 (MQ=255)
 aTTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCt                                                                                                                                               <  1:763158/151‑1 (MQ=255)
  ttGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTg                                                                                                                                              >  1:83824/1‑151 (MQ=255)
   tGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTg                                                                                                                                              <  2:1100713/150‑1 (MQ=255)
   tGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTg                                                                                                                                              >  2:115617/1‑150 (MQ=255)
       tttCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGAcc                                                                                                                                           >  2:373892/1‑149 (MQ=255)
       tttCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGAcc                                                                                                                                           >  2:171372/1‑149 (MQ=255)
        ttCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAg                                                                                                                                         <  2:1250908/150‑1 (MQ=255)
             gACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCAc                                                                                                                                   >  2:1183616/1‑151 (MQ=255)
             gACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGAAAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCAc                                                                                                                                   >  2:1230000/1‑151 (MQ=255)
               ccTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACtt                                                                                                                                 <  2:25185/151‑1 (MQ=255)
                  catcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTc                                                                                                                                >  2:1400528/1‑149 (MQ=255)
                  catcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTc                                                                                                                                <  2:126640/149‑1 (MQ=255)
                  catcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTc                                                                                                                                >  2:640409/1‑149 (MQ=255)
                  catcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCa                                                                                                                               <  1:622414/150‑1 (MQ=255)
                   atcaCTAAATGGTGCATTCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAgg                                                                                                                             >  1:859937/1‑151 (MQ=255)
                   atcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAgg                                                                                                                             <  1:465648/151‑1 (MQ=255)
                   atcaCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAgg                                                                                                                             <  1:1460680/151‑1 (MQ=255)
                     caCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAggg                                                                                                                            <  1:975536/150‑1 (MQ=255)
                     caCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGc                                                                                                                           <  1:677172/151‑1 (MQ=255)
                      aCTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCt                                                                                                                          >  2:316086/1‑151 (MQ=255)
                        tAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTa                                                                                                                         >  1:949990/1‑150 (MQ=255)
                         aaaTGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTa                                                                                                                         <  2:793886/149‑1 (MQ=255)
                               tGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCt                                                                                                                 >  2:216793/1‑151 (MQ=255)
                                    tCATTTTCACATCAATCGGATAGCGTTTACCTCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTTCCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCag                                                                                                             <  1:1145673/150‑1 (MQ=255)
                                    tCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTc                                                                                                               >  1:887993/1‑148 (MQ=255)
                                     cATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCagag                                                                                                           <  1:388586/151‑1 (MQ=255)
                                       ttttCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAggg                                                                                                         >  2:290237/1‑151 (MQ=255)
                                        tttCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAggg                                                                                                         >  2:313375/1‑150 (MQ=255)
                                         ttCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGa                                                                                                        <  2:364207/150‑1 (MQ=255)
                                         ttCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGAt                                                                                                       >  2:345661/1‑151 (MQ=255)
                                          tCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGAtt                                                                                                      >  2:1059833/1‑151 (MQ=255)
                                           cacaTCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGAttt                                                                                                     >  2:143912/1‑151 (MQ=255)
                                           cacaTCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGAtt                                                                                                      <  2:436743/150‑1 (MQ=255)
                                             caTCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTcc                                                                                                   <  2:732685/151‑1 (MQ=255)
                                             caTCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTcc                                                                                                   >  2:519296/1‑151 (MQ=255)
                                             caTCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTc                                                                                                    <  1:1284010/150‑1 (MQ=255)
                                                 aaTCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTc                                                                                               <  2:859306/151‑1 (MQ=255)
                                                    cGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAat                                                                                            <  1:401550/151‑1 (MQ=255)
                                                    cGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAat                                                                                            >  2:270173/1‑151 (MQ=255)
                                                    cGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAat                                                                                            <  1:1435076/151‑1 (MQ=255)
                                                     ggATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAatc                                                                                           <  2:1386018/151‑1 (MQ=255)
                                                        tAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAatccat                                                                                        >  1:395677/1‑151 (MQ=255)
                                                        tAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAatccat                                                                                        >  2:179036/1‑151 (MQ=255)
                                                         aGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAatccatc                                                                                       <  2:401805/151‑1 (MQ=255)
                                                          gCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAatccatcc                                                                                      <  1:460645/151‑1 (MQ=255)
                                                           cGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCg                                                                                     <  2:1304551/151‑1 (MQ=255)
                                                           cGTTTACCGCTAGCTTCTAAATCACCACTGATAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCg                                                                                     >  1:253624/1‑151 (MQ=255)
                                                            gTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCgg                                                                                    <  1:1230782/151‑1 (MQ=255)
                                                            gTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCgg                                                                                    <  2:1041084/151‑1 (MQ=255)
                                                            gTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCgg                                                                                    <  1:410172/151‑1 (MQ=255)
                                                            gTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCgg                                                                                    <  1:1305168/151‑1 (MQ=255)
                                                              ttACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCggg                                                                                   <  2:542016/150‑1 (MQ=255)
                                                                aCCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCg                                                                                 >  1:367084/1‑150 (MQ=255)
                                                                aCCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCg                                                                                 <  1:799322/150‑1 (MQ=255)
                                                                aCCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCg                                                                                 <  2:158719/150‑1 (MQ=255)
                                                                 ccGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGAt                                                                               <  2:76374/151‑1 (MQ=255)
                                                                    cTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGAt                                                                               >  2:1460169/1‑148 (MQ=255)
                                                                      aGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGa                                                                            <  1:167011/149‑1 (MQ=255)
                                                                         ttCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGt                                                                       >  1:515691/1‑151 (MQ=255)
                                                                          tCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGATGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGtt                                                                      >  1:927835/1‑151 (MQ=255)
                                                                           cTAAATCACCACTGCTAAAAGAGACGAAGTAAGGGGCTGGATTGGTCACTCGAAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGttt                                                                     >  2:14784/1‑151 (MQ=255)
                                                                            tAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGtttt                                                                    >  2:237075/1‑151 (MQ=255)
                                                                             aaaTCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGGTTGGTCACTCGTAATGACGCCTTACCTTCGGAGCCTGTCCAGAACCACTTCAGGGCTAACGGGGCTTCAGCGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGt                                                                       >  2:378325/1‑147 (MQ=255)
                                                                             aaaTCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGttt                                                                     <  1:640483/149‑1 (MQ=255)
                                                                             aaaTCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGtt                                                                      <  2:281199/148‑1 (MQ=255)
                                                                               aTCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTtata                                                                 <  2:820591/151‑1 (MQ=255)
                                                                                 caccacTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATAc                                                                <  1:171394/150‑1 (MQ=255)
                                                                                 caccacTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACg                                                               >  2:562286/1‑151 (MQ=255)
                                                                                    cacTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACgtgt                                                            <  2:332390/151‑1 (MQ=255)
                                                                                       tGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCg                                                         >  1:1044616/1‑151 (MQ=255)
                                                                                        gCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGc                                                          <  1:1183714/149‑1 (MQ=255)
                                                                                          tAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGaa                                                       >  1:1245503/1‑150 (MQ=255)
                                                                                           aaaaGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGaa                                                       <  1:270203/149‑1 (MQ=255)
                                                                                              agagACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGcc                                                  >  1:262353/1‑151 (MQ=255)
                                                                                                agaCGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAg                                                <  1:311873/151‑1 (MQ=255)
                                                                                                 gaCGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGt                                               <  2:411085/151‑1 (MQ=255)
                                                                                                 gaCGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGt                                               <  1:504816/151‑1 (MQ=255)
                                                                                                     tagtaAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTgc                                            >  1:298724/1‑150 (MQ=255)
                                                                                                         aaGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGcagcag                                       <  1:1400635/151‑1 (MQ=255)
                                                                                                          aGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTgcagca                                        <  1:299693/149‑1 (MQ=255)
                                                                                                            gggTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCt                                    <  1:352831/151‑1 (MQ=255)
                                                                                                                       gTCACTCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGAGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGa                         <  2:316814/151‑1 (MQ=255)
                                                                                                                            tCGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCttt                    >  2:523150/1‑151 (MQ=255)
                                                                                                                             cGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCttt                    <  1:745109/150‑1 (MQ=255)
                                                                                                                             cGTAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCtt                     <  2:843580/149‑1 (MQ=255)
                                                                                                                               tAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCt                 >  2:1360018/1‑151 (MQ=255)
                                                                                                                               tAATGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCt                 >  1:731561/1‑151 (MQ=255)
                                                                                                                                aaTGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCt                 <  1:777849/150‑1 (MQ=255)
                                                                                                                                 aTGACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGc               <  2:1256043/151‑1 (MQ=255)
                                                                                                                                   gACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCa              <  1:344576/150‑1 (MQ=255)
                                                                                                                                   gACGCCTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCAt             >  1:68819/1‑151 (MQ=255)
                                                                                                                                       ccTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTgg         >  2:1111605/1‑151 (MQ=255)
                                                                                                                                       ccTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTgg         <  1:143932/151‑1 (MQ=255)
                                                                                                                                       ccTTACCTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTgg         <  1:572786/151‑1 (MQ=255)
                                                                                                                                            ccTTCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGtttt     <  1:161154/150‑1 (MQ=255)
                                                                                                                                               tCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTtggt  <  1:221199/150‑1 (MQ=255)
                                                                                                                                               tCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTtggt  <  2:509677/150‑1 (MQ=255)
                                                                                                                                               tCTGAGCCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCCAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTtggt  <  2:590802/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGT  >  NC_000913/155550‑155842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: