Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,692,029	T→C	T275A (ACG→GCG)	uidC ←	putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,692,029	0	T	C	100.0%	374.8 / NA	98	T275A (ACG→GCG)	uidC	putative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/48);  total (50/48)

CGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACC  >  NC_000913/1691890‑1692174                                                                                                                                            |                                                                                                                                                  cGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGggct                                                                                                                                        <  2:178138/151‑1 (MQ=255)    tCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGggctggc                                                                                                                                     >  2:1354832/1‑151 (MQ=255)     cAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGggctggct                                                                                                                                    <  2:1263077/151‑1 (MQ=255)         aCTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGt                                                                                                                                 >  2:941443/1‑150 (MQ=255)         aCTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTa                                                                                                                                <  2:547924/151‑1 (MQ=255)           ttAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTAtt                                                                                                                              >  2:1217796/1‑151 (MQ=255)            tAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTAtt                                                                                                                              >  2:1409516/1‑150 (MQ=255)             aaTGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTAc                                                                                                                            <  1:654316/151‑1 (MQ=255)             aaTGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTAc                                                                                                                            >  1:361676/1‑151 (MQ=255)              aTGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTAtt                                                                                                                              >  2:906184/1‑148 (MQ=255)              aTGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACg                                                                                                                           <  2:904980/151‑1 (MQ=255)                gAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGAc                                                                                                                         >  2:978825/1‑151 (MQ=255)                gAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGAc                                                                                                                         >  2:333237/1‑151 (MQ=255)                gAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGAc                                                                                                                         >  2:571387/1‑151 (MQ=255)                gAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGAc                                                                                                                         >  2:450643/1‑151 (MQ=255)                   aaGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACtt                                                                                                                       >  2:163118/1‑150 (MQ=255)                     gggATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTaaa                                                                                                                    <  2:1019138/151‑1 (MQ=255)                     gggATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTaa                                                                                                                     >  1:155290/1‑150 (MQ=255)                       gATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAAcc                                                                                                                  <  1:119954/151‑1 (MQ=255)                          aGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCtt                                                                                                                <  1:1043655/150‑1 (MQ=255)                           gCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCg                                                                                                              >  2:1427041/1‑151 (MQ=255)                             cAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCg                                                                                                              >  1:994919/1‑149 (MQ=255)                                 gTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTg                                                                                                         <  1:1232901/150‑1 (MQ=255)                                  tCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTgcg                                                                                                       >  2:1469780/1‑151 (MQ=255)                                  tCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTgcg                                                                                                       >  2:832352/1‑151 (MQ=255)                                       aTCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTa                                                                                                   <  1:84127/150‑1 (MQ=255)                                        tCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaa                                                                                                  <  2:639847/150‑1 (MQ=255)                                         ccaccaCGGCCCCATTCATTGCATGGCCAAAATACCCTCCTCTGCCCAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTACCCAACGCCGCTTCTGTGGGCTGGCTGGTATTACGACTGAACCCTGCGAGCTGCGGGTaaaa                                                                                                <  2:778324/151‑1 (MQ=255)                                         ccaccaCGGCACCATTCATTGCATGGCGCAAATACCCGCCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaaa                                                                                                 <  1:169987/150‑1 (MQ=255)                                         ccaccaCGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaaaa                                                                                                <  1:382945/151‑1 (MQ=255)                                           accacGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGc                                                                                              >  2:389243/1‑151 (MQ=255)                                           accacGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGc                                                                                              >  2:547393/1‑151 (MQ=255)                                           accacGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGc                                                                                              >  1:409661/1‑151 (MQ=255)                                             cacGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCg                                                                                            >  2:451002/1‑151 (MQ=255)                                              acGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGACAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCg                                                                                            >  1:1223081/1‑150 (MQ=255)                                                 gCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCa                                                                                        <  2:771679/151‑1 (MQ=255)                                                  cACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCaa                                                                                       <  1:1032050/151‑1 (MQ=255)                                                        tcattGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTccc                                                                                  <  2:1251838/150‑1 (MQ=255)                                                         cattGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCt                                                                                <  1:1163578/151‑1 (MQ=255)                                                          attGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCtt                                                                               >  2:290660/1‑151 (MQ=255)                                                           ttGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCttt                                                                              >  2:2189/1‑151 (MQ=255)                                                            tGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCttt                                                                              <  2:866766/150‑1 (MQ=255)                                                               aTGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACTGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAAc                                                                          <  2:868389/151‑1 (MQ=255)                                                                tGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACg                                                                         >  2:661709/1‑151 (MQ=255)                                                                    gcAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGtata                                                                     <  1:1233154/151‑1 (MQ=255)                                                                     cAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGtata                                                                     >  1:877438/1‑150 (MQ=255)                                                                     cAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGtata                                                                     >  2:884284/1‑150 (MQ=255)                                                                      aaaTAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAAt                                                                   <  1:143717/151‑1 (MQ=255)                                                                        aTAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTc                                                                 <  1:1237078/151‑1 (MQ=255)                                                                         tAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCg                                                                >  2:761865/1‑151 (MQ=255)                                                                          aaCCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCgg                                                               >  1:1226870/1‑151 (MQ=255)                                                                           accaccACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCggg                                                              >  1:242451/1‑151 (MQ=255)                                                                               ccacTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGa                                                          <  2:974140/151‑1 (MQ=255)                                                                                cacTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAg                                                         <  2:296683/151‑1 (MQ=255)                                                                                cacTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAg                                                         <  1:1409624/151‑1 (MQ=255)                                                                                 acTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGc                                                        <  2:976573/151‑1 (MQ=255)                                                                                    gCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGt                                                     >  2:1358344/1‑151 (MQ=255)                                                                                     ccAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGt                                                     >  1:209173/1‑150 (MQ=255)                                                                                     ccAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTa                                                    <  1:547458/151‑1 (MQ=255)                                                                                     ccAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTa                                                    <  2:155269/151‑1 (MQ=255)                                                                                        aaTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGc                                                 <  2:994824/151‑1 (MQ=255)                                                                                         aTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCt                                                >  1:919312/1‑151 (MQ=255)                                                                                         aTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCt                                                >  2:1212948/1‑151 (MQ=255)                                                                                            cacTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCttt                                              <  2:796380/150‑1 (MQ=255)                                                                                              cTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtc                                            <  2:805960/150‑1 (MQ=255)                                                                                                tCCCCTCGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtct                                           <  1:1391950/149‑1 (MQ=255)                                                                                                tCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTtctc                                          <  1:1184058/150‑1 (MQ=255)                                                                                                  cccACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCg                                         >  1:113947/1‑149 (MQ=255)                                                                                                    cACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCgaga                                      >  2:799420/1‑150 (MQ=255)                                                                                                    cACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGaa                                     <  2:623478/151‑1 (MQ=255)                                                                                                      cGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAAt                                    >  2:428155/1‑150 (MQ=255)                                                                                                      cGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATa                                   <  1:739150/151‑1 (MQ=255)                                                                                                          gcATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt                               <  2:1232463/151‑1 (MQ=255)                                                                                                          gcATTCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt                               <  2:384253/151‑1 (MQ=255)                                                                                                             ttCCAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCT‑GCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAt                           >  1:1085690/1‑151 (MQ=255)                                                                                                               ccAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAt                           <  1:1089647/150‑1 (MQ=255)                                                                                                                cAGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCt                         <  2:512752/151‑1 (MQ=255)                                                                                                                 aGGTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAt                           <  1:1469888/148‑1 (MQ=255)                                                                                                                  ggTCAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGa                       <  1:1249231/151‑1 (MQ=255)                                                                                                                     cAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGAtt                     <  2:1093977/150‑1 (MQ=255)                                                                                                                     cAATTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTg                    >  1:169086/1‑151 (MQ=255)                                                                                                                      aaTTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTgg                   >  1:1403574/1‑151 (MQ=255)                                                                                                                      aaTTGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTgg                   >  2:1357455/1‑151 (MQ=255)                                                                                                                         tGTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGaa                 >  1:477712/1‑150 (MQ=255)                                                                                                                          gTCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAAc               <  2:1429729/151‑1 (MQ=255)                                                                                                                           tCCACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAAc               >  1:918105/1‑150 (MQ=255)                                                                                                                            ccACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACa              <  1:1197547/150‑1 (MQ=255)                                                                                                                             cACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATc            >  1:23276/1‑151 (MQ=255)                                                                                                                             cACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATc            <  1:333279/151‑1 (MQ=255)                                                                                                                             cACTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATc            >  1:527760/1‑151 (MQ=255)                                                                                                                              aCTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCt           >  2:1324035/1‑151 (MQ=255)                                                                                                                               cTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCtt          >  1:70082/1‑151 (MQ=255)                                                                                                                               cTAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCt           <  2:672066/150‑1 (MQ=255)                                                                                                                                tAACCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCttt         >  1:74941/1‑151 (MQ=255)                                                                                                                                  aCCAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCt       >  1:1136733/1‑151 (MQ=255)                                                                                                                                    cAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGt     >  2:1053001/1‑151 (MQ=255)                                                                                                                                    cAACGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGt     <  2:1238391/151‑1 (MQ=255)                                                                                                                                     aaCGCCGCTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGt     >  2:1458001/1‑150 (MQ=255)                                                                                                                                       cgccgcTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTAcc  >  1:19315/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                  CGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCCACCACGGCACCATTCATTGCATGGCGCAAATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTGTACC  >  NC_000913/1691890‑1692174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑