Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,768,998 T→C intergenic (‑313/‑76) ydiJ ← / → ydiK putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,768,9980TC99.1% 419.1 / ‑4.3 107intergenic (‑313/‑76)ydiJ/ydiKputative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (39/66);  total (39/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 110 total aligned reads displayed.

GGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGAT  >  NC_000913/1768850‑1769144
                                                                                                                                                    |                                                                                                                                                  
ggTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCcca                                                                                                                                                  <  2:1060430/151‑1 (MQ=255)
 gTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCccat                                                                                                                                                 <  2:19385/151‑1 (MQ=255)
 gTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCccat                                                                                                                                                 <  2:814650/151‑1 (MQ=255)
       gaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTAt                                                                                                                                           >  1:192406/1‑151 (MQ=255)
       gaacgTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTAt                                                                                                                                           <  2:14954/151‑1 (MQ=255)
         acgTAACAGCAGCACATGAAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATg                                                                                                                                          >  2:599792/1‑150 (MQ=255)
               ccGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGa                                                                                                                                   <  1:286192/151‑1 (MQ=255)
                  cagcaCATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGtt                                                                                                                                >  2:737073/1‑151 (MQ=255)
                   agcaCATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGttt                                                                                                                               <  2:1438325/151‑1 (MQ=255)
                   agcaCATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGttt                                                                                                                               >  2:823972/1‑151 (MQ=255)
                    gcaCATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTc                                                                                                                              >  2:677132/1‑151 (MQ=255)
                       caTGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTcgcg                                                                                                                           <  2:1081215/151‑1 (MQ=255)
                         tGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCgtg                                                                                                                         >  1:1145259/1‑151 (MQ=255)
                                tGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcg                                                                                                                   <  1:1178644/150‑1 (MQ=255)
                                  aaaTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcg                                                                                                                >  2:740587/1‑151 (MQ=255)
                                  aaaTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcg                                                                                                                <  2:909946/151‑1 (MQ=255)
                                  aaaTACAACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcg                                                                                                                >  2:785415/1‑151 (MQ=255)
                                   aaTACGACGAGTAAATCGTTTGCGTGTTGCGTGAGTTGTTGTGCCGCATTTTTTTCTAGCGCGCGGATCAGAATTAAGGGCAGGATGGGCCTGTTAAAAGCCGCTGAAAGGGCGCATGATTATGCAGGTGAGTGGGGCGTGTTCAcgtcg                                                                                                                <  2:895474/150‑1 (MQ=255)
                                   aaTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgc                                                                                                               <  2:717490/151‑1 (MQ=255)
                                    aTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcg                                                                                                              <  2:1397962/151‑1 (MQ=255)
                                      acgacgAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgt                                                                                                             <  1:67964/150‑1 (MQ=255)
                                       cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgtca                                                                                                           <  2:1008168/151‑2 (MQ=255)
                                         acgaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgac                                                                                                         <  2:340692/151‑1 (MQ=255)
                                           gaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgacg                                                                                                        >  1:1327811/1‑150 (MQ=255)
                                           gaGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgacg                                                                                                        >  1:476764/1‑150 (MQ=255)
                                            aGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt                                                                                                      <  2:1416760/151‑1 (MQ=255)
                                            aGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt                                                                                                      <  2:582839/151‑1 (MQ=255)
                                            aGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTCCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt                                                                                                      <  1:1187890/151‑1 (MQ=255)
                                             gTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAtt                                                                                                     >  2:474766/1‑151 (MQ=255)
                                              tAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAttt                                                                                                    >  2:609794/1‑151 (MQ=255)
                                               aaaTCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAttt                                                                                                    <  1:917687/150‑1 (MQ=255)
                                               aaaTCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAttt                                                                                                    <  2:172707/150‑1 (MQ=255)
                                               aaaTCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTg                                                                                                   <  1:368224/151‑1 (MQ=255)
                                                aaTCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGa                                                                                                  <  1:1182315/151‑1 (MQ=255)
                                                 aTCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGAc                                                                                                 <  1:1348059/151‑1 (MQ=255)
                                                  tCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACg                                                                                                >  2:228775/1‑151 (MQ=255)
                                                   cGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGc                                                                                               >  1:1034335/1‑151 (MQ=255)
                                                   cGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGc                                                                                               <  2:1177761/151‑1 (MQ=255)
                                                        gCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaa                                                                                          <  2:865599/151‑1 (MQ=255)
                                                         cGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaa                                                                                         <  1:3113/151‑1 (MQ=255)
                                                         cGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaa                                                                                          >  2:1421256/1‑150 (MQ=255)
                                                         cGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaa                                                                                          >  1:1458768/1‑150 (MQ=255)
                                                           tgtTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaaa                                                                                        >  2:710241/1‑150 (MQ=255)
                                                            gtTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAg                                                                                      <  1:67361/151‑1 (MQ=255)
                                                            gtTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAg                                                                                      <  1:355685/151‑1 (MQ=255)
                                                             ttGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAg                                                                                      >  2:634353/1‑150 (MQ=255)
                                                               gCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTg                                                                                   <  1:789377/151‑1 (MQ=255)
                                                                 cTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGa                                                                                  >  2:120589/1‑150 (MQ=255)
                                                                  tGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGaa                                                                                 >  2:1125973/1‑150 (MQ=255)
                                                                   gAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGaaa                                                                                >  2:1045434/1‑150 (MQ=255)
                                                                   gAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTACGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGaa                                                                                 <  1:1314858/149‑1 (MQ=255)
                                                                     gttgttGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAag                                                                              >  2:549441/1‑150 (MQ=255)
                                                                        gttgtACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAagtagt                                                                          <  2:192382/151‑1 (MQ=255)
                                                                        gttgtACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAagtag                                                                           <  2:160684/150‑1 (MQ=255)
                                                                          tgtACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGtt                                                                         <  1:1377394/150‑1 (MQ=255)
                                                                          tgtACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGtt                                                                         <  2:1297229/150‑1 (MQ=255)
                                                                          tgtACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGtt                                                                         <  2:35366/150‑1 (MQ=255)
                                                                             aCCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATg                                                                      <  2:1187847/150‑1 (MQ=255)
                                                                                acaTTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTaa                                                                  <  1:107893/151‑1 (MQ=255)
                                                                                  aTTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAAt                                                                >  1:1203815/1‑151 (MQ=255)
                                                                                  aTTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAAt                                                                <  2:183865/151‑1 (MQ=255)
                                                                                    ttttttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATg                                                               <  2:641199/150‑1 (MQ=255)
                                                                                     tttttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGt                                                              <  1:237551/150‑1 (MQ=255)
                                                                                     tttttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGt                                                              <  2:1080894/150‑1 (MQ=255)
                                                                                            aaCACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCa                                                        <  2:191819/149‑1 (MQ=255)
                                                                                             acacGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGcc                                                     >  1:1257556/1‑151 (MQ=255)
                                                                                               acGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCa                                                   <  2:951721/151‑1 (MQ=255)
                                                                                                   ccATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAggg                                                >  1:1177732/1‑150 (MQ=255)
                                                                                                    cATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAATATGTGAAAAGTGGTTATGGTAAATGTTCGTCAGCCCAGGGAt                                              >  1:669157/1‑151 (MQ=255)
                                                                                                    cATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGAGAt                                              <  2:73101/151‑1 (MQ=255)
                                                                                                     aTCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATg                                             >  1:889392/1‑151 (MQ=255)
                                                                                                       cAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTc                                           <  1:641918/151‑1 (MQ=255)
                                                                                                        aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTca                                          <  2:589478/151‑2 (MQ=255)
                                                                                                        aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCg                                          >  2:1047801/1‑151 (MQ=255)
                                                                                                          aaTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGc                                         >  1:305552/1‑150 (MQ=255)
                                                                                                           aTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGcac                                       <  2:340027/151‑1 (MQ=255)
                                                                                                             tAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGcaca                                      >  2:1275357/1‑150 (MQ=255)
                                                                                                              aaGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACaa                                     <  1:488230/150‑1 (MQ=255)
                                                                                                                  gCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAAtt                                  >  2:810045/1‑149 (MQ=255)
                                                                                                                  gCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAAtt                                  <  1:810123/149‑1 (MQ=255)
                                                                                                                  gCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCt                                <  2:1458661/151‑1 (MQ=255)
                                                                                                                   cAGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTg                               <  2:556186/151‑1 (MQ=255)
                                                                                                                    aGAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGc                              >  2:598833/1‑151 (MQ=255)
                                                                                                                     gAATCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCt                             >  1:612433/1‑151 (MQ=255)
                                                                                                                       aTCGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCttt                           <  2:175024/151‑1 (MQ=255)
                                                                                                                         cGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTc                          <  2:678115/150‑1 (MQ=255)
                                                                                                                         cGGCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCg                         <  1:626892/151‑1 (MQ=255)
                                                                                                                          ggCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCgg                        >  2:7939/1‑151 (MQ=255)
                                                                                                                          ggCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCg                         <  2:1327703/150‑1 (MQ=255)
                                                                                                                           gCCTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGt                       >  1:274145/1‑151 (MQ=255)
                                                                                                                            ccTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTg                      >  2:1429293/1‑151 (MQ=255)
                                                                                                                            ccTGTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTg                      <  2:809207/151‑1 (MQ=255)
                                                                                                                               gTTAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTg                   >  1:1456156/1‑151 (MQ=255)
                                                                                                                                ttAAAAACCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCt                    <  1:1160409/149‑1 (MQ=255)
                                                                                                                                  aaaaaCCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGttt                <  1:677205/151‑1 (MQ=255)
                                                                                                                                    aaaCCGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGttttt              <  1:740666/151‑1 (MQ=255)
                                                                                                                                       ccGCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGc           <  1:518382/151‑1 (MQ=255)
                                                                                                                                         gCTGAAATTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCca         <  1:1098611/151‑1 (MQ=255)
                                                                                                                                             aaaTTGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCcatcat     <  1:257840/151‑1 (MQ=255)
                                                                                                                                                ttGCCCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGAt  >  1:246411/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGAT  >  NC_000913/1768850‑1769144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: