Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,888,259 C→T A496A (GCG→GCA fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,2590CT98.9% 361.5 / ‑4.3 93A496A (GCG→GCAfadDacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (41/51);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGG  >  NC_000913/1888111‑1888406
                                                                                                                                                    |                                                                                                                                                   
gCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGc                                                                                                                                                   <  2:25707/151‑1 (MQ=255)
 cAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCt                                                                                                                                                  >  1:1163895/1‑151 (MQ=255)
   aaaTTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTc                                                                                                                                                >  2:776724/1‑151 (MQ=255)
   aaaTTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTc                                                                                                                                                >  2:673640/1‑151 (MQ=255)
    aaTTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTca                                                                                                                                               <  2:1288405/151‑1 (MQ=255)
    aaTTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTc                                                                                                                                                >  1:345178/1‑150 (MQ=255)
       ttttCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTcacc                                                                                                                                             >  1:302151/1‑150 (MQ=255)
        tttCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTcacca                                                                                                                                            <  2:1045785/150‑1 (MQ=255)
          tCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAGGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTg                                                                                                                                         >  2:281420/1‑151 (MQ=255)
          tCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTg                                                                                                                                         >  2:759939/1‑151 (MQ=255)
                   aGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGaa                                                                                                                                <  2:1006574/151‑1 (MQ=255)
                   aGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGaa                                                                                                                                <  2:725845/151‑1 (MQ=255)
                   aGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGa                                                                                                                                 >  1:376128/1‑150 (MQ=255)
                   aGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGa                                                                                                                                 <  2:452889/150‑1 (MQ=255)
                         cGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACg                                                                                                                          >  1:975505/1‑151 (MQ=255)
                         cGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACg                                                                                                                          >  2:207485/1‑151 (MQ=255)
                               cTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAAc                                                                                                                     >  1:1193828/1‑150 (MQ=255)
                                tcatcaCGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAAc                                                                                                                     >  1:1327886/1‑149 (MQ=255)
                                    caCGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCcgc                                                                                                               <  1:544525/151‑1 (MQ=255)
                                     aCGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCcgcg                                                                                                              <  1:59847/151‑1 (MQ=255)
                                     aCGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCcgcg                                                                                                              >  2:932265/1‑151 (MQ=255)
                                       gAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGAc                                                                                                            >  1:1119627/1‑151 (MQ=255)
                                       gAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGAc                                                                                                            >  2:380784/1‑151 (MQ=255)
                                        aaaCTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACt                                                                                                           <  1:839924/151‑1 (MQ=255)
                                         aaCTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACtt                                                                                                          <  1:617341/151‑1 (MQ=255)
                                             ccaccaGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAATTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTcc                                                                                                        <  1:348909/149‑1 (MQ=255)
                                             ccaccaGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTcc                                                                                                        <  2:670567/149‑1 (MQ=255)
                                             ccaccaGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCt                                                                                                       <  1:320275/150‑1 (MQ=255)
                                                ccaGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTa                                                                                                    <  2:654633/150‑1 (MQ=255)
                                                ccaGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTAc                                                                                                   <  2:741953/151‑1 (MQ=255)
                                                  aGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGc                                                                                                 >  2:810072/1‑151 (MQ=255)
                                                   gCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGcc                                                                                                <  1:111308/151‑1 (MQ=255)
                                                      tCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCa                                                                                               <  2:347161/149‑1 (MQ=255)
                                                       cGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAgg                                                                                             >  1:1416639/1‑150 (MQ=255)
                                                        ggTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGAt                                                                                           <  2:891885/151‑1 (MQ=255)
                                                        ggTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGAt                                                                                           <  1:71061/151‑1 (MQ=255)
                                                         gTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGAtg                                                                                          <  2:1027472/151‑1 (MQ=255)
                                                          tACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGAtgc                                                                                         >  2:533354/1‑151 (MQ=255)
                                                           aCTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGAtgct                                                                                        >  1:380624/1‑151 (MQ=255)
                                                                      cGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATgacgac                                                                              >  2:226969/1‑150 (MQ=255)
                                                                          aGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGCCGACATCtt                                                                         <  2:81477/151‑1 (MQ=255)
                                                                           gCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGCTGCTGCATGACGACATCTTc                                                                        >  2:641614/1‑151 (MQ=255)
                                                                                 ggcggcAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAAtctc                                                                  >  1:1049106/1‑151 (MQ=255)
                                                                                 ggcggcAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAAtctc                                                                  <  1:380834/151‑1 (MQ=255)
                                                                                   cggcAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGt                                                                >  2:912232/1‑151 (MQ=255)
                                                                                   cggcAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGt                                                                <  2:1119529/151‑1 (MQ=255)
                                                                                         aaaGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGAt                                                          <  2:121091/151‑1 (MQ=255)
                                                                                             tCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGAc                                                      >  1:629023/1‑151 (MQ=255)
                                                                                              cACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGAc                                                      <  1:960441/150‑1 (MQ=255)
                                                                                              cACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGAc                                                      <  1:97701/150‑1 (MQ=255)
                                                                                              cACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGAc                                                      <  1:1231645/150‑1 (MQ=255)
                                                                                              cACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACg                                                     >  1:626149/1‑151 (MQ=255)
                                                                                                ccAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGt                                                    <  2:993998/150‑1 (MQ=255)
                                                                                                ccAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCGTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGt                                                    <  2:481641/150‑1 (MQ=255)
                                                                                                 cAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGtt                                                   >  1:271337/1‑150 (MQ=255)
                                                                                                  aGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaa                                                 <  1:1216885/151‑1 (MQ=255)
                                                                                                  aGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTa                                                  >  1:120546/1‑150 (MQ=255)
                                                                                                   gTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaaa                                                <  1:1377451/151‑1 (MQ=255)
                                                                                                    tGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaaa                                                >  1:297918/1‑150 (MQ=255)
                                                                                                    tGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaaa                                                >  1:19695/1‑150 (MQ=255)
                                                                                                    tGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaaa                                                >  1:337843/1‑150 (MQ=255)
                                                                                                     gACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTaaaa                                               <  1:513516/150‑1 (MQ=255)
                                                                                                     gACTCTCCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAAc                                              >  1:849027/1‑151 (MQ=255)
                                                                                                      aCTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAAcc                                             <  2:1193018/151‑1 (MQ=255)
                                                                                                         ctTCGGTAAGCGATGGATCTTTTTTCGCTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCg                                            <  2:33838/149‑1 (MQ=255)
                                                                                                          ttCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGa                                          >  1:1155191/1‑150 (MQ=255)
                                                                                                           tCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGaa                                         >  2:1092105/1‑150 (MQ=255)
                                                                                                                 aGCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGaa                                  <  2:302115/151‑1 (MQ=255)
                                                                                                                  gCGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAAt                                 <  1:547433/151‑1 (MQ=255)
                                                                                                                   cGATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATc                                <  1:932354/151‑1 (MQ=255)
                                                                                                                    gATGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCa                               <  2:569731/151‑1 (MQ=255)
                                                                                                                     aTGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCACCAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCa                               <  2:1082242/150‑1 (MQ=255)
                                                                                                                     aTGGATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCAt                              <  2:1157639/151‑1 (MQ=255)
                                                                                                                       ggATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGt                            <  2:921820/151‑1 (MQ=255)
                                                                                                                       ggATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGt                            <  2:913648/151‑1 (MQ=255)
                                                                                                                        gATCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTc                           >  1:140037/1‑151 (MQ=255)
                                                                                                                          tCTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCtt                         >  1:949780/1‑151 (MQ=255)
                                                                                                                           cTTTTTTCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCttt                        >  1:802440/1‑151 (MQ=255)
                                                                                                                             tttttCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCtttt                       <  1:218000/150‑1 (MQ=255)
                                                                                                                             tttttCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCttt                        <  2:345135/149‑1 (MQ=255)
                                                                                                                               tttCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCttttt                      >  2:782801/1‑149 (MQ=255)
                                                                                                                                ttCACTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTAc                   >  2:570047/1‑151 (MQ=255)
                                                                                                                                   aCTACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGAt                <  2:609212/151‑1 (MQ=255)
                                                                                                                                     tACGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCg              >  1:649215/1‑151 (MQ=255)
                                                                                                                                      aCGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATc               <  1:1040649/149‑1 (MQ=255)
                                                                                                                                       cGAAGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGAc            >  2:837770/1‑151 (MQ=255)
                                                                                                                                         aaGATTTTCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACa           >  2:1251425/1‑150 (MQ=255)
                                                                                                                                              tttCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATgcg      <  1:533414/150‑1 (MQ=255)
                                                                                                                                               ttCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATgcgc     >  1:552900/1‑150 (MQ=255)
                                                                                                                                               ttCACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATgcgc     <  2:1163801/150‑1 (MQ=255)
                                                                                                                                                 cACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAgg  <  2:628949/151‑1 (MQ=255)
                                                                                                                                                 cACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAg   <  1:1446322/150‑1 (MQ=255)
                                                                                                                                                 cACTGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAg   <  2:1327778/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCTGACGGCGGCAAAAAGTCACCAGTGACTCTTCGGTAAGCGATGGATCTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGG  >  NC_000913/1888111‑1888406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: