Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,894,752 T→C intergenic (‑21/‑53) yoaH ← / → pabB UPF0181 family protein/aminodeoxychorismate synthase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,894,7520TC99.1% 414.8 / NA 111intergenic (‑21/‑53)yoaH/pabBUPF0181 family protein/aminodeoxychorismate synthase, subunit I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (60/50);  minor base A (1/0);  total (61/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 112 total aligned reads displayed.

ACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAT  >  NC_000913/1894606‑1894899
                                                                                                                                                  |                                                                                                                                                   
    aGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTa                                                                                                                                             <  1:1091861/151‑1 (MQ=255)
       tcttctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAatt                                                                                                                                          >  1:890422/1‑151 (MQ=255)
          tctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAattat                                                                                                                                        <  2:1012196/150‑1 (MQ=255)
          tctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAatta                                                                                                                                         <  1:1395772/149‑1 (MQ=255)
          tctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAatt                                                                                                                                          <  1:575457/148‑1 (MQ=255)
             gCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCa                                                                                                                                    <  1:215211/151‑1 (MQ=255)
              ccaccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCa                                                                                                                                    <  2:600803/150‑1 (MQ=255)
               caccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCAt                                                                                                                                   >  1:866417/1‑150 (MQ=255)
               caccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATg                                                                                                                                  >  2:1166213/1‑151 (MQ=255)
                  caGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTa                                                                                                                               <  2:1068455/151‑1 (MQ=255)
                   aGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTAc                                                                                                                              <  1:1285693/151‑1 (MQ=255)
                      gcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCg                                                                                                                           >  1:759898/1‑151 (MQ=255)
                       cgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCgg                                                                                                                          <  2:421080/151‑1 (MQ=255)
                         aTTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGc                                                                                                                         >  2:357701/1‑150 (MQ=255)
                          ttGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGcac                                                                                                                       >  2:348797/1‑151 (MQ=255)
                          cagCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGca                                                                                                                        >  2:944621/3‑150 (MQ=255)
                           tGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGcac                                                                                                                       >  1:1067616/1‑150 (MQ=255)
                            gCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGcaca                                                                                                                      <  2:1135308/150‑1 (MQ=255)
                            gCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACAt                                                                                                                     >  2:1264749/1‑151 (MQ=255)
                             ccTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATa                                                                                                                    <  1:1349062/151‑1 (MQ=255)
                               tGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGc                                                                                                                  >  1:754486/1‑151 (MQ=255)
                                ggCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGcc                                                                                                                 <  1:1120556/151‑1 (MQ=255)
                                 gCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCa                                                                                                                <  1:1259095/151‑1 (MQ=255)
                                   cGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                                                                              >  1:1362362/1‑151 (MQ=255)
                                       gctCATCCCTTGTGCCTTCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAAGTATTGCATGCTACCCGGCACATAGCCAGTag                                                                                                            <  2:798939/149‑1 (MQ=255)
                                       gctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTagag                                                                                                          <  1:408128/151‑1 (MQ=255)
                                       gctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTag                                                                                                            >  1:685383/1‑149 (MQ=255)
                                       gctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTag                                                                                                            >  1:866587/1‑149 (MQ=255)
                                       gctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTag                                                                                                            >  1:45880/1‑149 (MQ=255)
                                          cATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCa                                                                                                       <  1:618750/151‑1 (MQ=255)
                                           aTCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTc                                                                                                        >  2:1176106/1‑149 (MQ=255)
                                            tCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAgg                                                                                                     >  1:253606/1‑151 (MQ=255)
                                             cccTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGa                                                                                                    <  2:1323199/151‑1 (MQ=255)
                                              ccTCGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAACCATTGTTAACCCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCTCATAGCCAGTAGAGTCAgg                                                                                                     <  2:117041/149‑1 (MQ=255)
                                                 tgtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACt                                                                                                  <  2:883701/149‑1 (MQ=255)
                                                   tgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCCGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgat                                                                                               >  2:447152/1‑150 (MQ=255)
                                                     ccATCAGTTCCTGGATCCGCTCGACGGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGaa                                                                                            <  1:913312/151‑1 (MQ=255)
                                                       aTCAGTTCCTGGATTCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATGTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGa                                                                                          <  2:1307417/151‑1 (MQ=255)
                                                         cAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACg                                                                                        >  2:296492/1‑151 (MQ=255)
                                                          aGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGt                                                                                       >  1:699938/1‑151 (MQ=255)
                                                          aGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGt                                                                                       >  1:489377/1‑151 (MQ=255)
                                                           gTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCCGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGtt                                                                                      >  1:293665/1‑151 (MQ=255)
                                                              ccTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTAtc                                                                                   >  2:158402/1‑151 (MQ=255)
                                                                   aTCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCg                                                                              >  1:221976/1‑151 (MQ=255)
                                                                     ccGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCt                                                                            <  2:814515/151‑1 (MQ=255)
                                                                      cGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCtg                                                                           >  2:1186996/1‑151 (MQ=255)
                                                                      cGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCtg                                                                           <  1:984259/151‑1 (MQ=255)
                                                                       gCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCtgt                                                                          >  2:1471265/1‑151 (MQ=255)
                                                                        cTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCtgtg                                                                         >  1:1410585/1‑151 (MQ=255)
                                                                          cGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAt                                                                       <  2:789287/151‑1 (MQ=255)
                                                                              aGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttact                                                                   >  1:426864/1‑151 (MQ=255)
                                                                               gCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttactt                                                                  >  1:1106350/1‑151 (MQ=255)
                                                                                 tttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttacttt                                                                 >  2:653639/1‑150 (MQ=255)
                                                                                  ttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGACGACGTTATCTCCCGCTGTGAttacttta                                                                >  2:331425/1‑150 (MQ=255)
                                                                                   tttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttactttact                                                              >  1:481271/1‑151 (MQ=255)
                                                                                    ttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActc                                                             >  1:1213297/1‑151 (MQ=255)
                                                                                      gctgctGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct                                                            <  1:944710/150‑1 (MQ=255)
                                                                                      gctgctGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTg                                                           >  2:287620/1‑151 (MQ=255)
                                                                                      gctgctGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTg                                                           <  1:194792/151‑1 (MQ=255)
                                                                                        tgctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGc                                                         >  1:53358/1‑151 (MQ=255)
                                                                                        tgctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGc                                                         >  1:239742/1‑151 (MQ=255)
                                                                                         gctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTgg                                                          <  1:528124/149‑1 (MQ=255)
                                                                                         gctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGc                                                         <  2:866499/150‑1 (MQ=255)
                                                                                              tCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAgg                                                   >  2:1384464/1‑151 (MQ=255)
                                                                                              tCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAgg                                                   >  1:1001498/1‑151 (MQ=255)
                                                                                                  gggTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGAcg                                                <  1:180573/150‑1 (MQ=255)
                                                                                                    gtgagtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGAcgccg                                             >  2:923816/1‑151 (MQ=255)
                                                                                                           aaGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAAtt                                      >  1:1100560/1‑151 (MQ=255)
                                                                                                             ggTAAACCTGCAAACATAGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTATAGTCAGGACTGATGAAGACGTTATCTCCCGCTATCGCTGCTTTACTCTCGCGTCATGACGCCGCTGAGtttt                                    <  2:460122/151‑1 (MQ=255)
                                                                                                               tAAACCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAt                                  >  2:1111027/1‑151 (MQ=255)
                                                                                                                aaaCCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAtt                                 >  1:759986/1‑151 (MQ=255)
                                                                                                                aaaCCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAtt                                 >  2:197415/1‑151 (MQ=255)
                                                                                                                 aaCCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAttt                                >  1:596287/1‑151 (MQ=255)
                                                                                                                 aaCCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAttt                                <  2:430711/151‑1 (MQ=255)
                                                                                                                 aaCCTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAttt                                >  1:890594/1‑151 (MQ=255)
                                                                                                                   ccTGCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtct                              >  1:673324/1‑151 (MQ=255)
                                                                                                                      gCAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtctc                             <  1:166649/149‑1 (MQ=255)
                                                                                                                       cAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCg                          <  1:1314991/151‑1 (MQ=255)
                                                                                                                       cAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCg                          >  2:410795/1‑151 (MQ=255)
                                                                                                                       cAAACATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCg                          <  1:469912/151‑1 (MQ=255)
                                                                                                                         aaCATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCt                        >  2:1275006/1‑151 (MQ=255)
                                                                                                                          aCATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCtt                       <  1:331467/151‑1 (MQ=255)
                                                                                                                           cATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTa                      <  2:614066/151‑1 (MQ=255)
                                                                                                                           cATTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTa                      <  2:890334/151‑1 (MQ=255)
                                                                                                                            aTTGTTAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTaa                     <  1:389797/151‑1 (MQ=255)
                                                                                                                                ttAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGcc                  >  2:1472444/1‑150 (MQ=255)
                                                                                                                                ttAACTCCTGCTAAATTGCTGGCGCTAATCATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACGCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGcc                  <  1:111937/150‑1 (MQ=255)
                                                                                                                                 tAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCAc                >  2:1395047/1‑151 (MQ=255)
                                                                                                                                 tAACTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCAc                >  1:1082308/1‑151 (MQ=255)
                                                                                                                                 tAACTCCTGCTAAATTGATGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCa                 >  1:996018/1‑150 (MQ=255)
                                                                                                                                  aaCTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTAACCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCAcc               >  2:161337/1‑151 (MQ=255)
                                                                                                                                   aCTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCt              <  1:1264850/151‑1 (MQ=255)
                                                                                                                                    cTCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTg             >  2:439765/1‑151 (MQ=255)
                                                                                                                                     tCCTGCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGc            >  2:1315298/1‑151 (MQ=255)
                                                                                                                                         gCTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCCCCTGCCGTg        <  2:1025088/151‑1 (MQ=255)
                                                                                                                                          cTAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTgg       >  1:448812/1‑151 (MQ=255)
                                                                                                                                           tAAATTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGt         <  2:65803/148‑1 (MQ=255)
                                                                                                                                            aaaTTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc     <  2:1285539/151‑1 (MQ=255)
                                                                                                                                             aaTTGCTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCg    <  2:685307/151‑1 (MQ=255)
                                                                                                                                               ttgctgGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAt  >  1:982451/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAT  >  NC_000913/1894606‑1894899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: