Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,273,106 C→T T248I (ACC→ATC)  yejA → microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,273,1060CT98.8% 323.4 / ‑4.4 82T248I (ACC→ATC) yejAmicrocin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (39/42);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

TAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACATCATCAAA  >  NC_000913/2272960‑2273251
                                                                                                                                                  |                                                                                                                                                 
tAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCa                                                                                                                                                 <  1:1265053/149‑1 (MQ=255)
 aGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGAcatcat                                                                                                                                                <  1:167560/149‑1 (MQ=255)
 aGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTc                                                                                                                                              >  2:291551/1‑151 (MQ=255)
   cGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGc                                                                                                                                            <  1:1209835/151‑1 (MQ=255)
   cGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGc                                                                                                                                            >  1:1453500/1‑151 (MQ=255)
    gACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCt                                                                                                                                           <  2:26902/151‑1 (MQ=255)
        cGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGa                                                                                                                                       <  1:184289/151‑1 (MQ=255)
           tCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGatt                                                                                                                                     >  1:136462/1‑150 (MQ=255)
           tCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGatt                                                                                                                                     >  2:300484/1‑150 (MQ=255)
            cGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGatt                                                                                                                                     <  2:892492/149‑1 (MQ=255)
                  gCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTc                                                                                                                             >  1:146730/1‑151 (MQ=255)
                   ccTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCg                                                                                                                            >  1:834972/1‑151 (MQ=255)
                   ccTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCg                                                                                                                            <  1:220663/151‑1 (MQ=255)
                     tCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCgat                                                                                                                          >  1:960211/1‑151 (MQ=255)
                        gCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCgatgat                                                                                                                       <  1:72389/151‑1 (MQ=255)
                         cTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCgatgat                                                                                                                       >  1:1393081/1‑150 (MQ=255)
                           tGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAAt                                                                                                                    >  2:311917/1‑151 (MQ=255)
                                gTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGc                                                                                                               <  2:1418043/151‑1 (MQ=255)
                                     ccGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCttt                                                                                                           <  2:1147409/150‑1 (MQ=255)
                                     ccGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCttt                                                                                                           <  1:1433865/150‑1 (MQ=255)
                                       gTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGaa                                                                                                        <  2:418335/151‑1 (MQ=255)
                                          ccGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCg                                                                                                     <  1:743072/151‑1 (MQ=255)
                                            gcgTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGtt                                                                                                   >  2:253794/1‑151 (MQ=255)
                                             cgTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGttt                                                                                                  <  1:414527/151‑1 (MQ=255)
                                                tACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTaa                                                                                                >  2:88612/1‑150 (MQ=255)
                                                 aCGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTaa                                                                                                >  1:9931/1‑149 (MQ=255)
                                                     ccTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAgg                                                                                          <  1:708262/151‑1 (MQ=255)
                                                          aaaaTGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCt                                                                                     >  2:144631/1‑151 (MQ=255)
                                                               ggggCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGa                                                                                 <  1:769301/150‑1 (MQ=255)
                                                               ggggCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGAt                                                                                <  2:1191815/151‑1 (MQ=255)
                                                                gggCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGAtt                                                                               >  1:1241577/1‑151 (MQ=255)
                                                                  gCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTg                                                                             <  2:146710/151‑1 (MQ=255)
                                                                   cAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGc                                                                            >  1:460194/1‑151 (MQ=255)
                                                                    aaaaTATTGTCTATTCCCGTGTGAACGATTACTGGGCAGCAAACTTACCGATAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCg                                                                           >  2:220782/1‑151 (MQ=255)
                                                                    aaaaTATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCg                                                                           >  2:971963/1‑151 (MQ=255)
                                                                    aaaaTATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCg                                                                           >  2:532871/1‑151 (MQ=255)
                                                                      aaTATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGt                                                                          >  1:1456110/1‑150 (MQ=255)
                                                                      aaTATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGt                                                                          >  2:1453946/1‑150 (MQ=255)
                                                                      aaTATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGt                                                                          <  2:681548/150‑1 (MQ=255)
                                                                       atatTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTAt                                                                        <  1:496733/151‑1 (MQ=255)
                                                                       atatTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTAt                                                                        <  2:136444/151‑1 (MQ=255)
                                                                        tatTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATg                                                                       >  1:340177/1‑151 (MQ=255)
                                                                          ttGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATgg                                                                      >  2:1440147/1‑150 (MQ=255)
                                                                            gTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGaaa                                                                   <  2:1185917/151‑1 (MQ=255)
                                                                              cTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAac                                                                 >  2:1265690/1‑151 (MQ=255)
                                                                                 ttCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAacgac                                                              >  2:413843/1‑151 (MQ=255)
                                                                                  tCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAacgacg                                                             >  2:255477/1‑151 (MQ=255)
                                                                                    ccGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGcc                                                           <  1:308470/151‑1 (MQ=255)
                                                                                     cGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGcc                                                           >  2:298454/1‑150 (MQ=255)
                                                                                     cGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCa                                                          <  2:933328/151‑1 (MQ=255)
                                                                                     cGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCa                                                          <  1:428935/151‑1 (MQ=255)
                                                                                       tgtgAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCaa                                                         >  1:1061972/1‑150 (MQ=255)
                                                                                       tgtgAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCaa                                                         >  1:1417403/1‑150 (MQ=255)
                                                                                           aaaGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACt                                                    >  1:708353/1‑151 (MQ=255)
                                                                                            aaGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTg                                                   <  1:300519/151‑1 (MQ=255)
                                                                                             aGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTgg                                                  <  1:1233241/151‑1 (MQ=255)
                                                                                                 tACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGcc                                               >  1:480112/1‑150 (MQ=255)
                                                                                                     gggCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCAcgcg                                          <  1:956971/151‑1 (MQ=255)
                                                                                                     gggCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCAcgcg                                          <  2:590135/151‑1 (MQ=255)
                                                                                                       gcagcaAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAACAGGTGCCTTTGATTTGCGTATGGAAAACGAAGCCAAAAACTGGGCCACGCGtt                                        <  2:1151592/151‑1 (MQ=255)
                                                                                                         agcaAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTtat                                      >  2:1269802/1‑151 (MQ=255)
                                                                                                          gcaAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTtat                                      >  2:314260/1‑150 (MQ=255)
                                                                                                            aaaCTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATAcc                                   >  2:1415312/1‑151 (MQ=255)
                                                                                                              aCTTACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCgg                                 <  1:977668/151‑1 (MQ=255)
                                                                                                                 tACCGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTa                               >  2:12034/1‑150 (MQ=255)
                                                                                                                   ccGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTaaaa                            <  1:960388/151‑1 (MQ=255)
                                                                                                                   ccGGTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTaaaa                            <  2:976073/151‑1 (MQ=255)
                                                                                                                     ggTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAAt                          <  2:834892/151‑1 (MQ=255)
                                                                                                                      gTAAACCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAAtt                         <  1:412768/151‑1 (MQ=255)
                                                                                                                         aaCCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCg                      <  2:9932/151‑1 (MQ=255)
                                                                                                                         aaCCGTGGAAGCTGGAATTTCGACACCATTCGCTACGATTATTCCCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAGCGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCg                      <  1:390075/151‑1 (MQ=255)
                                                                                                                          aCCGTGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCg                      >  2:1049549/1‑150 (MQ=255)
                                                                                                                              tGGACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATaa                  >  2:171410/1‑150 (MQ=255)
                                                                                                                               ggACGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATaaa                 <  1:1065346/150‑1 (MQ=255)
                                                                                                                                  cGCTGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATGTCGATAAAAAAt             <  2:867069/151‑1 (MQ=255)
                                                                                                                                     tGGAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATAc           >  1:1216566/1‑150 (MQ=255)
                                                                                                                                      ggAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATAcat         <  1:259000/151‑1 (MQ=255)
                                                                                                                                      ggAATTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATAc           <  1:328920/149‑1 (MQ=255)
                                                                                                                                         aTTTCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATAcatcat      <  1:253819/151‑1 (MQ=255)
                                                                                                                                           ttCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACatcatc     >  1:1287317/1‑150 (MQ=255)
                                                                                                                                            tCGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACAtcatca    <  2:1292873/150‑1 (MQ=255)
                                                                                                                                             cGACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACATCATCaaa  <  2:880028/151‑1 (MQ=255)
                                                                                                                                              gACATCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACATCATCaa   >  1:510651/1‑149 (MQ=255)
                                                                                                                                               acatCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACATCATCaa   >  1:203803/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCGATGATAATGTCGCCTTTGAAGCGTTTAAAGCAGGTGCCTTTGATTTGCGTATGGAAAACGACGCCAAAAACTGGGCCACGCGTTATACCGGTAAAAATTTCGATAAAAAATACATCATCAAA  >  NC_000913/2272960‑2273251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: