Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,127	T→C	V288A (GTA→GCA)	glpB →	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,127	0	T	C	100.0%	308.7 / NA	85	V288A (GTA→GCA)	glpB	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/44);  total (41/44)

ACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATG  >  NC_000913/2354981‑2355267                                                                                                                                                   |                                                                                                                                             aCCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTg                                                                                                                                          >  1:166778/1‑151 (MQ=255) aCCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTg                                                                                                                                          <  1:1345113/151‑1 (MQ=255)  ccTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGTATTCGTCTGCAAAACCAGTTACTGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGTTAACAGTGACCTGTAAAAATGGCGCAGTg                                                                                                                                          >  2:619734/1‑150 (MQ=255)     tGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACg                                                                                                                                      >  2:1000043/1‑151 (MQ=255)      gTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACg                                                                                                                                      >  2:1269350/1‑150 (MQ=255)      gTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGa                                                                                                                                     <  2:349606/151‑1 (MQ=255)      gTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGa                                                                                                                                     <  1:1383298/151‑1 (MQ=255)        tCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGaa                                                                                                                                    >  1:1451666/1‑150 (MQ=255)        tCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGaa                                                                                                                                    >  1:728678/1‑150 (MQ=255)        tCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGaa                                                                                                                                    <  2:1206461/150‑1 (MQ=255)        tCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGaa                                                                                                                                    <  2:51185/150‑1 (MQ=255)         cACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAAt                                                                                                                                  >  1:1001415/1‑151 (MQ=255)         cACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAAt                                                                                                                                  <  1:768249/151‑1 (MQ=255)         cACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAAt                                                                                                                                  >  1:248998/1‑151 (MQ=255)          aCTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATc                                                                                                                                 <  1:315331/151‑1 (MQ=255)            tGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTg                                                                                                                               >  2:126973/1‑151 (MQ=255)             gATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTgg                                                                                                                              <  1:1222688/151‑1 (MQ=255)             gATGCTTTTGCCAACGCTGCCGACTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCt                                                                                                                                <  1:438785/149‑1 (MQ=255)              aTGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGa                                                                                                                             <  1:307917/151‑1 (MQ=255)                  ttttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCg                                                                                                                         >  1:904719/1‑151 (MQ=255)                   tttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCg                                                                                                                         <  1:60389/150‑1 (MQ=255)                   tttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGc                                                                                                                        >  1:157731/1‑151 (MQ=255)                   tttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGc                                                                                                                        >  1:1136917/1‑151 (MQ=255)                   tttGCCAACGCTGCCGCCTTCAGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGc                                                                                                                        >  2:252074/1‑151 (MQ=255)                    ttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGc                                                                                                                        >  2:816382/1‑150 (MQ=255)                    ttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGc                                                                                                                        >  1:1071550/1‑150 (MQ=255)                     tGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCa                                                                                                                       >  2:1389467/1‑150 (MQ=255)                     tGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCa                                                                                                                       <  2:628522/150‑1 (MQ=255)                     tGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCa                                                                                                                       <  1:309446/150‑1 (MQ=255)                         aaCGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCa                                                                                                                   >  1:846685/1‑150 (MQ=255)                         aaCGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCAc                                                                                                                  <  1:1207516/151‑1 (MQ=255)                             cTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGcc                                                                                                               >  1:559908/1‑150 (MQ=255)                                 cgccTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGatat                                                                                                          >  2:160399/1‑151 (MQ=255)                                      tCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAAGGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTcc                                                                                                       <  2:832217/149‑1 (MQ=255)                                       ccGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCt                                                                                                    >  1:1114197/1‑151 (MQ=255)                                       ccGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCt                                                                                                    <  1:889927/151‑1 (MQ=255)                                        cGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTa                                                                                                   <  1:429571/151‑1 (MQ=255)                                         gTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTa                                                                                                   >  2:982594/1‑150 (MQ=255)                                         gTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTAc                                                                                                  <  2:45154/151‑1 (MQ=255)                                           gCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGt                                                                                                >  2:753756/1‑151 (MQ=255)                                                 cATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGt                                                                                          <  1:227860/151‑1 (MQ=255)                                                    tCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGttt                                                                                        <  2:616909/150‑1 (MQ=255)                                                    tCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTc                                                                                       >  1:766915/1‑151 (MQ=255)                                                      gTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTcgc                                                                                     <  1:709387/151‑1 (MQ=255)                                                           cAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTtc                                                                                <  1:244680/151‑1 (MQ=255)                                                            aaaaCCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTtct                                                                               <  2:290387/151‑1 (MQ=255)                                                            aaaaCCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTtc                                                                                <  1:1293919/150‑1 (MQ=255)                                                             aaaCCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTtctc                                                                              <  2:1347690/151‑1 (MQ=255)                                                              aaCCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCg                                                                             >  1:1462190/1‑151 (MQ=255)                                                                ccAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGcc                                                                           >  2:883874/1‑151 (MQ=255)                                                                   gTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAg                                                                         <  1:1421152/150‑1 (MQ=255)                                                                   gTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGc                                                                        >  1:1185681/1‑151 (MQ=255)                                                                              aGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCtt                                                             <  2:1347172/151‑1 (MQ=255)                                                                               gTTTGTGCGCCAGGGTGGCGGTTTGAGGCCGGGCGATGAAGTAAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCtt                                                             <  2:166757/150‑1 (MQ=255)                                                                                tttGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCttt                                                            >  2:786918/1‑150 (MQ=255)                                                                                   gtgCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTg                                                        <  2:1463599/151‑1 (MQ=255)                                                                                   gtgCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTg                                                        <  2:922334/151‑1 (MQ=255)                                                                                     gcgcCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGc                                                      >  1:187866/1‑151 (MQ=255)                                                                                           gggTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACt                                                 <  1:477970/150‑1 (MQ=255)                                                                                           gggTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTg                                                <  2:1393795/151‑1 (MQ=255)                                                                                             gTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGt                                              <  1:496079/151‑1 (MQ=255)                                                                                               ggCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTa                                             <  2:294470/150‑1 (MQ=255)                                                                                                  gtgtGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCg                                          <  2:394794/150‑1 (MQ=255)                                                                                                        aTGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGt                                    >  2:602075/1‑150 (MQ=255)                                                                                                        aTGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTa                                   <  1:1104037/151‑1 (MQ=255)                                                                                                           ccGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACg                                >  1:1119155/1‑151 (MQ=255)                                                                                                            cGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACgg                               >  2:993504/1‑151 (MQ=255)                                                                                                             gggCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGc                              <  2:837259/151‑1 (MQ=255)                                                                                                             gggCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGc                              <  2:908682/151‑1 (MQ=255)                                                                                                               gCGATGAAGTGAAAAAAGTGACCTGTAAAACTGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAt                            >  1:1387439/1‑151 (MQ=255)                                                                                                                cGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAtt                           <  2:248974/151‑1 (MQ=255)                                                                                                                cGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAtt                           <  2:983956/151‑1 (MQ=255)                                                                                                                cGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAtt                           <  2:1239490/151‑1 (MQ=255)                                                                                                                cGATGAAGTGAAAAAAGGGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAtt                           <  1:786997/151‑1 (MQ=255)                                                                                                                  aTGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCg                         >  1:1265265/1‑151 (MQ=255)                                                                                                                   tGAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCg                         >  1:535865/1‑150 (MQ=255)                                                                                                                    gAAGTGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCga                        <  2:157710/150‑1 (MQ=255)                                                                                                                        tGAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGcc                   >  1:120775/1‑151 (MQ=255)                                                                                                                         gAAAAAAGTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCg                  >  1:141683/1‑151 (MQ=255)                                                                                                                              aaGTGACCTGTAAAAATGGCGCAGTGAGCGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATtc              >  1:1170714/1‑150 (MQ=255)                                                                                                                                gTGACCTGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCg           <  2:1417328/151‑1 (MQ=255)                                                                                                                                      tGTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTg     >  2:141959/1‑151 (MQ=255)                                                                                                                                       gTAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTg     >  1:848966/1‑150 (MQ=255)                                                                                                                                        tAAAAATGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGAt   >  1:406407/1‑151 (MQ=255)                                                                                                                                              tGGCGCAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAAAGGCATTCGAGAGCCGATTCTCGGCCTTGAtg  >  2:513847/1‑146 (MQ=255)                                                                                                                                                   |                                                                                                                                             ACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATG  >  NC_000913/2354981‑2355267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑