Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,453,901 G→A pseudogene (1101/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,453,9010GA100.0% 313.4 / NA 81pseudogene (1101/2646 nt)yfcUpseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/43);  total (38/43)

CTGATAGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGGCCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTG  >  NC_000913/2453754‑2454044
                                                                                                                                                   |                                                                                                                                               
cTGATAGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAAcctgacct                                                                                                                                              >  1:1107441/1‑151 (MQ=255)
 tGATAGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAacctgacctg                                                                                                                                             >  2:165449/1‑151 (MQ=255)
  gATAGTTTTCATCCCCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAAcctgacctgg                                                                                                                                            <  2:404693/151‑1 (MQ=255)
   aTAGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTggg                                                                                                                                           <  1:693587/151‑1 (MQ=255)
     aGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCg                                                                                                                                         >  1:153916/1‑151 (MQ=255)
        tttCATCCCCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAg                                                                                                                                       <  2:1411216/150‑1 (MQ=255)
         ttCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTg                                                                                                                                     >  2:115746/1‑151 (MQ=255)
            atcaCCCAGCGCACCGCCATATAGCGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTg                                                                                                                                     <  1:121350/148‑1 (MQ=255)
              caCCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGt                                                                                                                                 >  2:742059/1‑150 (MQ=255)
                 ccAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATgg                                                                                                                             <  1:881066/151‑1 (MQ=255)
                  cAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGc                                                                                                                            >  2:1012847/1‑151 (MQ=255)
                    gcgcACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCa                                                                                                                           <  1:409652/150‑1 (MQ=255)
                     cgcACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCAt                                                                                                                          <  1:303290/150‑1 (MQ=255)
                       cACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCg                                                                                                                        >  2:864965/1‑150 (MQ=255)
                       cACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCg                                                                                                                        >  2:969318/1‑150 (MQ=255)
                          cGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGc                                                                                                                    <  2:59431/151‑1 (MQ=255)
                          cGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGc                                                                                                                    <  2:932238/151‑1 (MQ=255)
                            ccATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCgg                                                                                                                  <  2:499354/151‑1 (MQ=255)
                            ccATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCgg                                                                                                                  <  2:787477/151‑1 (MQ=255)
                             cATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGt                                                                                                                 <  1:221789/151‑1 (MQ=255)
                              atatAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTg                                                                                                                <  1:15427/151‑1 (MQ=255)
                                ataACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCt                                                                                                              <  2:751897/151‑1 (MQ=255)
                                 taACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTg                                                                                                             >  2:863564/1‑151 (MQ=255)
                                   aCGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGAt                                                                                                           <  1:1116053/151‑1 (MQ=255)
                                    cGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATg                                                                                                          <  2:1173825/151‑1 (MQ=255)
                                     gACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGt                                                                                                         <  1:765987/151‑1 (MQ=255)
                                      aCCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTc                                                                                                        <  1:1148645/151‑1 (MQ=255)
                                      aCCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTc                                                                                                        >  1:1005316/1‑151 (MQ=255)
                                      aCCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTc                                                                                                        >  1:401467/1‑151 (MQ=255)
                                       ccAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTc                                                                                                        >  1:648602/1‑150 (MQ=255)
                                       ccAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTc                                                                                                        >  1:1470086/1‑150 (MQ=255)
                                        cAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCat                                                                                                      <  2:1424363/151‑1 (MQ=255)
                                         aGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCata                                                                                                     <  2:997859/151‑1 (MQ=255)
                                         aGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCata                                                                                                     <  1:419963/151‑1 (MQ=255)
                                                aGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGc                                                                                              >  1:1095078/1‑151 (MQ=255)
                                                    aTGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCt                                                                                          <  1:182345/151‑1 (MQ=255)
                                                    aTGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGCGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCAcc                                                                                           >  2:248238/1‑150 (MQ=255)
                                                      gCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTgg                                                                                        <  2:403004/151‑1 (MQ=255)
                                                      gCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTg                                                                                         <  2:429680/150‑1 (MQ=255)
                                                           aggaggCGTCAGCACCAGAGAAAAATCCCCCCTCGACATGGCGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATGTTCCTGCACCTGGCCg                                                                                     >  2:957382/1‑149 (MQ=255)
                                                             gaggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCgttc                                                                                  >  1:1286325/1‑150 (MQ=255)
                                                              aggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCgttctg                                                                                >  1:551944/1‑151 (MQ=255)
                                                               ggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCgttctg                                                                                >  2:466844/1‑150 (MQ=255)
                                                                   tCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGttctt                                                                           >  1:457661/1‑151 (MQ=255)
                                                                    cAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGttctt                                                                           <  1:165469/150‑1 (MQ=255)
                                                                        aCCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATg                                                                      <  2:617209/151‑1 (MQ=255)
                                                                         ccAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGc                                                                     >  1:1405302/1‑151 (MQ=255)
                                                                           agagAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCg                                                                    >  1:544164/1‑150 (MQ=255)
                                                                              gaAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAata                                                                <  1:898292/151‑1 (MQ=255)
                                                                              gaAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAat                                                                 >  2:499852/1‑150 (MQ=255)
                                                                                  aaTCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCa                                                            <  1:1037154/151‑1 (MQ=255)
                                                                                      cccccTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGt                                                        >  2:1246945/1‑151 (MQ=255)
                                                                                         ccTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTAcc                                                     >  1:1011289/1‑151 (MQ=255)
                                                                                          cTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCg                                                    >  1:1000580/1‑151 (MQ=255)
                                                                                          cTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGGCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTAcc                                                     >  2:1174559/1‑150 (MQ=255)
                                                                                            cGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCgg                                                   <  1:1086350/150‑1 (MQ=255)
                                                                                            cGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCgg                                                   <  2:476759/150‑1 (MQ=255)
                                                                                            cGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCg                                                    <  2:1394752/149‑1 (MQ=255)
                                                                                            cGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGga                                                  <  2:1367439/151‑1 (MQ=255)
                                                                                            cGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGga                                                  <  1:280331/151‑1 (MQ=255)
                                                                                             gACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCgg                                                   >  1:638315/1‑149 (MQ=255)
                                                                                               cATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGAc                                               <  1:1122361/151‑1 (MQ=255)
                                                                                                aTGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACg                                              <  2:572939/151‑1 (MQ=255)
                                                                                                 tggtggCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACgg                                             >  2:616249/1‑151 (MQ=255)
                                                                                                  ggtggCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGa                                            <  1:960306/151‑1 (MQ=255)
                                                                                                     ggCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATc                                         >  2:449713/1‑151 (MQ=255)
                                                                                                       ccccACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCAc                                       <  2:753115/151‑1 (MQ=255)
                                                                                                        cccACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCAcc                                      >  2:1459261/1‑151 (MQ=255)
                                                                                                         ccACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCAcc                                      >  1:1006833/1‑150 (MQ=255)
                                                                                                          cACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCa                                     >  2:1057158/1‑150 (MQ=255)
                                                                                                             tctTGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCa                                     >  2:915896/1‑147 (MQ=255)
                                                                                                                tGCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATc                                <  1:753171/149‑1 (MQ=255)
                                                                                                                 gCGGACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTg                             <  1:40629/151‑1 (MQ=255)
                                                                                                                   ggACGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGaa                           <  2:221795/151‑1 (MQ=255)
                                                                                                                     aCGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAAt                          >  1:601388/1‑150 (MQ=255)
                                                                                                                      cGTCCCATCATGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATa                         >  2:96300/1‑150 (MQ=255)
                                                                                                                            atcatGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACg                  >  1:1060921/1‑151 (MQ=255)
                                                                                                                               atGATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTc               >  1:809727/1‑151 (MQ=255)
                                                                                                                                 gATCTTATAGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTcc              >  2:476527/1‑150 (MQ=255)
                                                                                                                                      tataGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAg            <  1:1239082/147‑1 (MQ=255)
                                                                                                                                       ataGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCACGCTCCCGCATACCAACCGGTCCacgagc        <  2:866248/150‑6 (MQ=255)
                                                                                                                                        taGCGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGa       >  1:226309/1‑150 (MQ=255)
                                                                                                                                           cGAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCt   <  1:730489/151‑1 (MQ=255)
                                                                                                                                            gAACCTGACCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTg  <  1:96312/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTGATAGTTTTCATCACCCAGCGCACCGCCATATAACGACCAGCCGTTAGCGATGCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGATCTTATAGCGAACCTGGCCTGGGCGAGTGAGGTATGGCATCGAGGCGGTGCTGATGTCATATTCCTGCACCTGGCCGTTCTGTTCTTCAATGCGAATATGCAACGTACCGGAGACGGAATCACCAAGATCCTGAATACGAAACGGTCCAGCCGGAACCTG  >  NC_000913/2453754‑2454044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: