Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,458,447	T→C	H238R (CAC→CGC)	fadJ ←	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,458,447	0	T	C	100.0%	310.7 / NA	85	H238R (CAC→CGC)	fadJ	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/44);  total (41/44)

CTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGTGCGGA  >  NC_000913/2458300‑2458593                                                                                                                                                    |                                                                                                                                                   ctacGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTgcgt                                                                                                                                                  <  2:784420/150‑1 (MQ=255)   acGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCt                                                                                                                                               <  2:419443/151‑1 (MQ=255)   acGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCt                                                                                                                                               <  1:357/151‑1 (MQ=255)   acGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCt                                                                                                                                               <  2:1342701/151‑1 (MQ=255)      cAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGtt                                                                                                                                            >  1:612624/1‑151 (MQ=255)      cAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGtt                                                                                                                                            <  1:631750/151‑1 (MQ=255)      cAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGtt                                                                                                                                            >  1:857816/1‑151 (MQ=255)        gcgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGtttt                                                                                                                                          <  1:801577/151‑1 (MQ=255)         cgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTc                                                                                                                                         <  2:614892/151‑1 (MQ=255)           ccTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCtt                                                                                                                                       <  1:656365/151‑1 (MQ=255)              gCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGcc                                                                                                                                    >  2:836469/1‑151 (MQ=255)              gCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGcc                                                                                                                                    >  1:714938/1‑151 (MQ=255)                gATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCg                                                                                                                                   >  1:754969/1‑150 (MQ=255)                gATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCg                                                                                                                                   >  2:397763/1‑150 (MQ=255)                         gTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                                          >  1:1070108/1‑150 (MQ=255)                         gTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGa                                                                                                                         <  2:132440/151‑1 (MQ=255)                           cATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAc                                                                                                                       >  2:1188840/1‑151 (MQ=255)                             tCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAc                                                                                                                       >  1:758006/1‑149 (MQ=255)                             tCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAc                                                                                                                       >  1:1230101/1‑149 (MQ=255)                             tCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAc                                                                                                                       >  1:117290/1‑149 (MQ=255)                               gCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAcagca                                                                                                                   <  1:1246/151‑1 (MQ=255)                                ccAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAAcagc                                                                                                                    >  1:1055991/1‑149 (MQ=255)                                  aGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAgcg                                                                                                                >  2:1021904/1‑151 (MQ=255)                                   gTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAgcgc                                                                                                               >  2:1011723/1‑151 (MQ=255)                                     tCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCa                                                                                                              <  1:745900/150‑1 (MQ=255)                                     tCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCa                                                                                                              <  2:415255/150‑1 (MQ=255)                                      cGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCAc                                                                                                             <  2:970094/150‑1 (MQ=255)                                        ccAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGa                                                                                                           <  1:53412/150‑1 (MQ=255)                                          aaaCGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCt                                                                                                        >  2:1268808/1‑151 (MQ=255)                                          aaaCGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCt                                                                                                        >  1:743642/1‑151 (MQ=255)                                            aCGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGAcc                                                                                                         >  2:748197/1‑148 (MQ=255)                                             cGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAAc                                                                                                     <  1:1033956/151‑1 (MQ=255)                                              gCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACg                                                                                                    <  1:14560/151‑1 (MQ=255)                                               cccGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACgg                                                                                                   <  2:335592/151‑1 (MQ=255)                                                  gAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGccc                                                                                                <  1:444869/151‑1 (MQ=255)                                                   aGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcccc                                                                                               <  2:834343/151‑1 (MQ=255)                                                    gCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCg                                                                                              <  2:622077/151‑1 (MQ=255)                                                    gCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCg                                                                                              >  1:733353/1‑151 (MQ=255)                                                     cTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGc                                                                                             >  1:1066497/1‑151 (MQ=255)                                                        cGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCa                                                                                           <  1:1283449/150‑1 (MQ=255)                                                        cGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCa                                                                                           <  2:9878/150‑1 (MQ=255)                                                         ggCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGcc                                                                                            >  1:1314146/1‑148 (MQ=255)                                                             tCATACCCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATa                                                                                      <  2:757928/150‑1 (MQ=255)                                                              cATAACCGCGGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTACCGGCCCCGCCCGAATa                                                                                      <  2:1230001/149‑1 (MQ=255)                                                               aTAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGc                                                                                   >  1:412148/1‑151 (MQ=255)                                                               aTAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGc                                                                                   <  1:1228246/151‑1 (MQ=255)                                                                 aaCCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTc                                                                                 >  2:704012/1‑151 (MQ=255)                                                                  aCCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTc                                                                                 >  1:265838/1‑150 (MQ=255)                                                                     gctgctGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGt                                                                             >  2:1325245/1‑151 (MQ=255)                                                                          tgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAgg                                                                        >  2:1095991/1‑151 (MQ=255)                                                                           ggTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAgg                                                                        <  1:1120029/150‑1 (MQ=255)                                                                              gCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTag                                                                     <  2:1416188/150‑1 (MQ=255)                                                                                  tGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGCCGCCGGATAATTGCCTTGAGTTCTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTag                                                                     <  2:1234383/146‑1 (MQ=255)                                                                                   gcgcTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGc                                                                <  1:638261/150‑1 (MQ=255)                                                                                    cgcTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGCGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCgg                                                              >  2:123801/1‑151 (MQ=255)                                                                                    cgcTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCgg                                                              >  1:795882/1‑151 (MQ=255)                                                                                         aTCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAg                                                          <  2:25432/150‑1 (MQ=255)                                                                                                ttCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGgcgc                                                  >  1:694030/1‑151 (MQ=255)                                                                                                   aacaacCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTcc                                               >  2:1416738/1‑151 (MQ=255)                                                                                                     caacCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCctt                                             >  2:1065978/1‑151 (MQ=255)                                                                                                      aacCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttc                                            >  2:60130/1‑151 (MQ=255)                                                                                                      aacCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttc                                            <  1:767210/151‑1 (MQ=255)                                                                                                      aacCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCctt                                             <  2:693952/150‑1 (MQ=255)                                                                                                       acCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttct                                           >  1:65080/1‑151 (MQ=255)                                                                                                        ccTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttct                                           <  1:836550/150‑1 (MQ=255)                                                                                                        ccTCCACGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttctt                                          <  2:833565/151‑1 (MQ=255)                                                                                                            cAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGcc                                      <  1:41292/151‑1 (MQ=255)                                                                                                              ggATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCa                                     <  1:286499/150‑1 (MQ=255)                                                                                                                   cGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCaa                               <  1:520211/151‑1 (MQ=255)                                                                                                                    gTTCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAAc                              >  1:584360/1‑151 (MQ=255)                                                                                                                      tCTGTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACa                             <  1:724733/150‑1 (MQ=255)                                                                                                                       cTGTCGCCGGATAACTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACa                             >  2:1191613/1‑149 (MQ=255)                                                                                                                         gTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAg                            <  2:878734/148‑1 (MQ=255)                                                                                                                         gTCGCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCg                          <  2:1103689/150‑1 (MQ=255)                                                                                                                            gCCGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCtt                      >  1:747265/1‑151 (MQ=255)                                                                                                                             ccGGATAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTc                     <  1:1191713/151‑1 (MQ=255)                                                                                                                                 aTAATTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCcagc                 >  1:532453/1‑151 (MQ=255)                                                                                                                                   aaTTGCCTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCcagc                 <  1:1268910/149‑1 (MQ=255)                                                                                                                                        ccTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATg           >  1:127802/1‑150 (MQ=255)                                                                                                                                         cTTGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATgg          >  2:1456637/1‑150 (MQ=255)                                                                                                                                           tGAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGt       >  1:730214/1‑151 (MQ=255)                                                                                                                                            gAGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGt       <  2:1207851/150‑1 (MQ=255)                                                                                                                                            gAGTTTTGCGTGCTGTTTTCTTGCCGGCCATTTTGAACAGCAGCGCACGTCCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAg        <  2:1241932/149‑1 (MQ=255)                                                                                                                                             aGTTTTGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGTGc     >  2:431488/1‑151 (MQ=255)                                                                                                                                                 ttGCGTTCTGTTTTCTTGCCGCCCATTTTGAACGGCAGCGCACGACCTACCGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGTGCGGa  <  1:748275/150‑1 (MQ=255)                                                                                                                                                 ttGCGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGTGCGGa  >  1:864654/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCGGCTTCCAGCAGAATGGAGTGCGGA  >  NC_000913/2458300‑2458593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑