Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,261	T→C	L47P (CTG→CCG)	yafD →	endo/exonuclease/phosphatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,261	0	T	C	100.0%	364.6 / NA	96	L47P (CTG→CCG)	yafD	endo/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/55);  total (41/55)

AAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCG  >  NC_000913/231113‑231404                                                                                                                                                     |                                                                                                                                                aaGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCgg                                                                                                                                               <  1:1185878/151‑1 (MQ=255)  aGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATTGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGgt                                                                                                                                              <  2:1177881/151‑1 (MQ=255)  aGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGgt                                                                                                                                              <  1:175592/151‑1 (MQ=255)       aaaGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGa                                                                                                                                         <  1:223114/151‑1 (MQ=255)        aaGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGa                                                                                                                                         >  1:332302/1‑150 (MQ=255)               aaaaaaCACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATAc                                                                                                                                 <  2:980602/151‑1 (MQ=255)                  aaaCACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACaaa                                                                                                                              <  2:934044/151‑1 (MQ=255)                     cacCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAAc                                                                                                                             >  2:322719/1‑149 (MQ=255)                     cacCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAg                                                                                                                           <  1:479200/151‑1 (MQ=255)                       ccTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCa                                                                                                                         <  1:894668/151‑1 (MQ=255)                       ccTATGCCATCCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTGCCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGc                                                                                                                          <  1:1023939/150‑1 (MQ=255)                          aTGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAAcg                                                                                                                      <  1:178226/151‑1 (MQ=255)                            gCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAAcgcg                                                                                                                    <  2:15597/151‑1 (MQ=255)                            gCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAAcgc                                                                                                                     <  1:193943/150‑1 (MQ=255)                             ccATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACgcgc                                                                                                                   <  1:1417649/151‑1 (MQ=255)                             ccATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACgcgc                                                                                                                   >  1:366923/1‑151 (MQ=255)                              cATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCt                                                                                                                  >  2:161163/1‑151 (MQ=255)                              cATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCt                                                                                                                  <  2:674850/151‑1 (MQ=255)                                 gcgcTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGaa                                                                                                               <  2:920070/151‑1 (MQ=255)                                     gaTGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATgg                                                                                                            >  2:1314859/2‑150 (MQ=255)                                      aTGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGt                                                                                                           <  2:1244286/150‑1 (MQ=255)                                         ttGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTc                                                                                                       >  1:1048847/1‑151 (MQ=255)                                          tGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTg                                                                                                                 <  2:809373/140‑1 (MQ=255)                                          tGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTg                                                                                                                 >  1:809452/1‑140 (MQ=255)                                          tGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTc                                                                                                       <  2:316233/150‑1 (MQ=255)                                           gCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCgg                                                                                                     >  2:560046/1‑151 (MQ=255)                                            ccGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCgg                                                                                                     >  2:667168/1‑150 (MQ=255)                                             cGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGt                                                                                                    >  1:178489/1‑150 (MQ=255)                                             cGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGt                                                                                                    >  1:317930/1‑150 (MQ=255)                                              ggACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTAt                                                                                                  >  1:369451/1‑151 (MQ=255)                                               gACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTAtt                                                                                                 <  1:954493/151‑1 (MQ=255)                                                  aaCCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTaa                                                                                               <  2:1341237/150‑1 (MQ=255)                                                      tGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAAc                                                                                          >  2:551019/1‑151 (MQ=255)                                                       gCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGaa                                                                                           <  1:73314/149‑1 (MQ=255)                                                       gCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACt                                                                                         <  1:1397436/151‑1 (MQ=255)                                                       gCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACt                                                                                         <  2:1023191/151‑1 (MQ=255)                                                        cGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTa                                                                                        <  1:1221334/151‑1 (MQ=255)                                                         ggAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTAc                                                                                       >  2:392636/1‑151 (MQ=255)                                                          gAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACg                                                                                      <  1:1113372/151‑1 (MQ=255)                                                           aaaGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACgg                                                                                     <  1:285988/151‑1 (MQ=255)                                                             aGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGATGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCa                                                                                   <  2:982626/151‑1 (MQ=255)                                                             aGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCa                                                                                   <  2:817294/151‑1 (MQ=255)                                                              ggATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCa                                                                                   >  1:507577/1‑150 (MQ=255)                                                                aTCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCaa                                                                                  >  2:1163211/1‑149 (MQ=255)                                                                  cTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCaa                                                                                  >  1:184710/1‑147 (MQ=255)                                                                   ttACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATg                                                                             >  2:162510/1‑151 (MQ=255)                                                                    tACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGGACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGc                                                                            >  1:563474/1‑151 (MQ=255)                                                                    tACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGAt                                                                              <  2:185225/149‑1 (MQ=255)                                                                    tACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATg                                                                             <  2:771893/150‑1 (MQ=255)                                                                    tACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGc                                                                            >  1:697684/1‑151 (MQ=255)                                                                    tACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGc                                                                            >  1:1410146/1‑151 (MQ=255)                                                                     aCCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGc                                                                            >  1:777728/1‑150 (MQ=255)                                                                      ccgccgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAg                                                                                <  2:114868/145‑1 (MQ=255)                                                                      ccgccgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGcac                                                                          <  1:1234273/151‑1 (MQ=255)                                                                       cgccgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGcac                                                                          >  1:257857/1‑150 (MQ=255)                                                                         ccgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATAGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATc                                                                       >  1:1397750/1‑151 (MQ=255)                                                                          cgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCt                                                                      <  1:143953/151‑1 (MQ=255)                                                                          cgGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCt                                                                      >  1:414269/1‑151 (MQ=255)                                                                              gTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGgtg                                                                  <  1:1181178/151‑1 (MQ=255)                                                                              gTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGgt                                                                   >  2:1441031/1‑150 (MQ=255)                                                                                  tttGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGgtgt                                                                 <  1:153547/148‑1 (MQ=255)                                                                                   ttGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTAt                                                              <  1:1468722/150‑1 (MQ=255)                                                                                     gCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTg                                                            <  2:450462/150‑1 (MQ=255)                                                                                     gCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTg                                                            <  2:1324675/150‑1 (MQ=255)                                                                                          cATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGaa                                                      <  2:1202213/151‑1 (MQ=255)                                                                                            tCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAgc                                                    >  1:107681/1‑151 (MQ=255)                                                                                              ggCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAgcgc                                                  <  2:1246630/151‑1 (MQ=255)                                                                                                ccAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAgcgc                                                  >  1:860305/1‑149 (MQ=255)                                                                                                 cAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAgcgc                                                  >  2:372827/1‑148 (MQ=255)                                                                                                 cAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAgcgc                                                  >  1:694952/1‑148 (MQ=255)                                                                                                  aGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGAc                                              >  1:1182412/1‑151 (MQ=255)                                                                                                        tACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGc                                         <  1:282340/150‑1 (MQ=255)                                                                                                        tACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGc                                         <  2:666486/150‑1 (MQ=255)                                                                                                         accaccTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCa                                       <  1:781809/151‑1 (MQ=255)                                                                                                           caccTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGcc                                        >  1:1364059/1‑148 (MQ=255)                                                                                                                ggggAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGtt                                  <  2:1461183/149‑1 (MQ=255)                                                                                                                ggggAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGtt                                  <  1:370344/149‑1 (MQ=255)                                                                                                                  ggAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTa                              <  1:522193/151‑1 (MQ=255)                                                                                                                   gAACCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTAc                             <  2:1034702/151‑1 (MQ=255)                                                                                                                    aaCCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTAc                             >  2:1287676/1‑150 (MQ=255)                                                                                                                    aaCCGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTAc                             >  2:809607/1‑150 (MQ=255)                                                                                                                       cGTTAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGtt                         <  1:974152/151‑1 (MQ=255)                                                                                                                         ttAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGttt                        <  2:631736/150‑1 (MQ=255)                                                                                                                          tAAGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTg                       <  2:465173/150‑1 (MQ=255)                                                                                                                           aaGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCg                     >  2:686829/1‑151 (MQ=255)                                                                                                                            aGTACCGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGa                    <  1:918737/151‑1 (MQ=255)                                                                                                                                ccGAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGc                >  2:892913/1‑151 (MQ=255)                                                                                                                                  gAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTa              <  2:1265811/151‑1 (MQ=255)                                                                                                                                  gAAGAGCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTa              <  1:1468574/151‑1 (MQ=255)                                                                                                                                   aaGAGCGTATTCGGATCCCGGGGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTa              >  2:205691/1‑150 (MQ=255)                                                                                                                                    agagCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAAc            <  1:322759/151‑1 (MQ=255)                                                                                                                                      agCGTATTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACt           >  1:647104/1‑150 (MQ=255)                                                                                                                                           aTTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTg     >  1:543851/1‑151 (MQ=255)                                                                                                                                           aTTCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTg     <  1:392689/151‑1 (MQ=255)                                                                                                                                            ttCGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTgc    >  2:1416263/1‑151 (MQ=255)                                                                                                                                              cGGATCCCGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTgccg  >  1:640458/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                AAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCG  >  NC_000913/231113‑231404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑