Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,476,595	A→G	pseudogene (12/15 nt)	pawZ →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,476,595	0	A	G	98.8%	312.6 / ‑3.7	85	pseudogene (12/15 nt)	pawZ	tRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (43/41);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTC  >  NC_000913/2476450‑2476741                                                                                                                                                  |                                                                                                                                                   aTGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATc                                                                                                                                               <  1:1317865/151‑1 (MQ=255)   ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCt                                                                                                                                             >  2:661522/1‑151 (MQ=255)   ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCt                                                                                                                                             >  1:1353956/1‑151 (MQ=255)       atatCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgt                                                                                                                                          <  2:988768/150‑1 (MQ=255)       atatCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgt                                                                                                                                          <  2:51566/150‑1 (MQ=255)        tatCGTGATCATTGTGAATTTAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACAACATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgtg                                                                                                                                         <  1:197193/150‑1 (MQ=255)         atCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGtt                                                                                                                                       >  1:761198/1‑151 (MQ=255)            gTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTAt                                                                                                                                    <  2:856338/151‑1 (MQ=255)              gACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATcc                                                                                                                                  >  1:274197/1‑151 (MQ=255)               aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATcc                                                                                                                                  <  2:49013/150‑1 (MQ=255)                 cATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTa                                                                                                                               <  2:1284694/151‑1 (MQ=255)                 cATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTa                                                                                                                               <  1:497852/151‑1 (MQ=255)                      tgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAAcc                                                                                                                          >  2:72066/1‑151 (MQ=255)                       gAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAAcc                                                                                                                          <  1:1344394/150‑1 (MQ=255)                          ttCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGAAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACAt                                                                                                                      >  2:599424/1‑151 (MQ=255)                           tCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACAt                                                                                                                      <  2:1215426/150‑1 (MQ=255)                            cAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaa                                                                                                                    <  2:380067/151‑1 (MQ=255)                             aaaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaa                                                                                                                    <  2:1395274/150‑1 (MQ=255)                              aaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaaaa                                                                                                                  <  2:498140/151‑1 (MQ=255)                                 aTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAAcc                                                                                                               >  1:433392/1‑151 (MQ=255)                                   aaGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGt                                                                                                             >  2:1453840/1‑151 (MQ=255)                                    aGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGt                                                                                                             >  2:1299962/1‑150 (MQ=255)                                      ctctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaa                                                                                                           >  2:871933/1‑150 (MQ=255)                                      ctctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaa                                                                                                           >  1:106476/1‑150 (MQ=255)                                       tctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaat                                                                                                         >  2:466810/1‑151 (MQ=255)                                          tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatta                                                                                                       >  1:1251546/1‑150 (MQ=255)                                          tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt                                                                                                        <  1:588632/149‑1 (MQ=255)                                          tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt                                                                                                        <  1:1266373/149‑1 (MQ=255)                                          tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaat                                                                                                         <  1:206235/148‑1 (MQ=255)                                           aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa                                                                                                      >  2:1125023/1‑150 (MQ=255)                                           aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa                                                                                                      >  1:808246/1‑150 (MQ=255)                                                gcgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGa                                                                                                <  2:1319951/151‑1 (MQ=255)                                                 cgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGaa                                                                                               <  1:14242/151‑1 (MQ=255)                                                      aTGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGc                                                                                          >  2:486281/1‑151 (MQ=255)                                                        gggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCa                                                                                         <  1:401856/150‑1 (MQ=255)                                                         ggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAgg                                                                                       <  2:660440/151‑1 (MQ=255)                                                          gTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGc                                                                                      >  1:1313642/1‑151 (MQ=255)                                                          gTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGc                                                                                      <  1:230093/151‑1 (MQ=255)                                                            aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGcc                                                                                     <  2:335962/150‑1 (MQ=255)                                                            aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGcc                                                                                     <  2:922178/150‑1 (MQ=255)                                                            aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  1:1059934/1‑151 (MQ=255)                                                            aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  2:498399/1‑151 (MQ=255)                                                             aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  1:203122/1‑150 (MQ=255)                                                             aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa                                                                                   <  1:1166597/151‑1 (MQ=255)                                                             aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa                                                                                   <  2:725724/151‑1 (MQ=255)                                                               aTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt                                                                                 >  1:1240817/1‑151 (MQ=255)                                                               aTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt                                                                                 <  1:26039/151‑1 (MQ=255)                                                                       tAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcac                                                                          >  1:1381159/1‑150 (MQ=255)                                                                        aaaaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca                                                                         <  2:591817/150‑1 (MQ=255)                                                                          aaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACAcc                                                                       >  1:587935/1‑150 (MQ=255)                                                                          aaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACAcc                                                                       >  1:206937/1‑150 (MQ=255)                                                                                   cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACt                                                                <  1:991777/148‑1 (MQ=255)                                                                                   cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCg                                                              >  2:1063943/1‑150 (MQ=255)                                                                                   cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGt                                                             >  1:870267/1‑151 (MQ=255)                                                                                    acaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTg                                                            >  1:803681/1‑151 (MQ=255)                                                                                          aaaaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGtttt                                                       >  2:145702/1‑150 (MQ=255)                                                                                            aaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAtt                                                    >  1:425794/1‑151 (MQ=255)                                                                                            aaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAtt                                                    >  2:833502/1‑151 (MQ=255)                                                                                              aaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTc                                                   >  2:584672/1‑150 (MQ=255)                                                                                                  ccATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTa                                               <  2:702838/150‑1 (MQ=255)                                                                                                  ccATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTAt                                              <  1:268997/151‑1 (MQ=255)                                                                                                     ttCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGa                                            >  2:853948/1‑150 (MQ=255)                                                                                                        caatcaatCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTg                                        >  1:812543/1‑151 (MQ=255)                                                                                                         aatcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTgg                                       >  1:1252132/1‑151 (MQ=255)                                                                                                         aatcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTgg                                       >  2:1009464/1‑151 (MQ=255)                                                                                                           tcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGtt                                     >  1:930796/1‑151 (MQ=255)                                                                                                              atcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGttttt                                  >  2:374217/1‑151 (MQ=255)                                                                                                               tcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGttttt                                  >  1:608653/1‑150 (MQ=255)                                                                                                                ccccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTa                                 <  2:1353850/150‑1 (MQ=255)                                                                                                                ccccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTAc                                >  2:1053797/1‑151 (MQ=255)                                                                                                                 cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTAcc                               >  2:1368539/1‑151 (MQ=255)                                                                                                                        gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtctc                        >  1:614382/1‑151 (MQ=255)                                                                                                                        gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtctc                        <  1:661595/151‑1 (MQ=255)                                                                                                                        gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtct                         <  2:1235051/150‑1 (MQ=255)                                                                                                                            cAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAg                    <  2:936281/151‑1 (MQ=255)                                                                                                                              aGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                  <  2:8360/151‑1 (MQ=255)                                                                                                                              aGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                  <  2:500736/151‑1 (MQ=255)                                                                                                                                tAGATGTCTGCAGGGGACGCCATCCTTGTTTTTATCCCTAAAACCACATAAAAACCGAAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGc                   >  1:256873/1‑148 (MQ=255)                                                                                                                                 aGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGa               <  2:331748/151‑1 (MQ=255)                                                                                                                                   aTGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGata             <  2:1137798/151‑1 (MQ=255)                                                                                                                                    tGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGata             >  1:1250606/1‑150 (MQ=255)                                                                                                                                      tCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataat           >  1:326245/1‑150 (MQ=255)                                                                                                                                         gCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataata          <  1:790942/148‑1 (MQ=255)                                                                                                                                         gCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataata          <  2:1398364/148‑1 (MQ=255)                                                                                                                                              ggACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTc  <  2:1109989/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTC  >  NC_000913/2476450‑2476741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑