Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,841	T→C	L69P (CTG→CCG)	tktB →	transketolase 2, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,841	0	T	C	100.0%	339.2 / NA	91	L69P (CTG→CCG)	tktB	transketolase 2, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/37);  total (54/37)

ACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACA  >  NC_000913/2579700‑2579985                                                                                                                                              |                                                                                                                                                 aCTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTAc                                                                                                                                           >  1:245757/1‑149 (MQ=255)    ctGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGt                                                                                                                                        <  1:875652/149‑1 (MQ=255)        tCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCt                                                                                                                                   >  1:1463319/1‑150 (MQ=255)         cATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTAc                                                                                                                                 >  2:1043612/1‑151 (MQ=255)           tCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACAt                                                                                                                               >  1:789388/1‑151 (MQ=255)           tCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACAt                                                                                                                               >  1:1286917/1‑151 (MQ=255)             ccGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCt                                                                                                                             >  1:640884/1‑151 (MQ=255)              cGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTg                                                                                                                            >  2:454634/1‑151 (MQ=255)               ggCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGa                                                                                                                           <  2:1426990/151‑1 (MQ=255)               ggCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGa                                                                                                                           <  2:414571/151‑1 (MQ=255)                gcgcgcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGa                                                                                                                           <  1:1208357/150‑1 (MQ=255)                 cgcgcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGAc                                                                                                                          <  1:1457948/150‑1 (MQ=255)                   cgcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCgg                                                                                                                       >  1:957565/1‑151 (MQ=255)                    gcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGt                                                                                                                      >  1:998254/1‑151 (MQ=255)                      tgATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTATAGTTTGCTACATCTGACCGGtt                                                                                                                     >  2:101260/2‑150 (MQ=255)                          gggCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGAc                                                                                                                <  1:1037952/151‑1 (MQ=255)                            gCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGtt                                                                                                                     >  2:7198/1‑144 (MQ=255)                            gCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGtt                                                                                                                     <  1:7198/144‑1 (MQ=255)                             cATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTg                                                                                                             <  2:643691/151‑1 (MQ=255)                             cATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTg                                                                                                             <  1:956115/151‑1 (MQ=255)                               tGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGcc                                                                                                           >  2:1230756/1‑151 (MQ=255)                                   tGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTg                                                                                                       >  2:954685/1‑151 (MQ=255)                                    gATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTg                                                                                                       <  2:579890/150‑1 (MQ=255)                                     atatTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTgg                                                                                                      >  2:1332322/1‑150 (MQ=255)                                          gCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGCCCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaa                                                                                                 <  2:1299203/150‑1 (MQ=255)                                          gCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaac                                                                                                <  1:477074/151‑1 (MQ=255)                                          gCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaa                                                                                                 <  1:1045692/150‑1 (MQ=255)                                          gCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaa                                                                                                 <  2:936695/150‑1 (MQ=255)                                           ccGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTAGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGCCCTGCCGTTGcacgaac                                                                                                >  2:1042273/1‑150 (MQ=255)                                             gAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactg                                                                                              >  2:98510/1‑150 (MQ=255)                                             gAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactg                                                                                              >  1:70453/1‑150 (MQ=255)                                                 tGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactgaaga                                                                                          >  2:1456936/1‑150 (MQ=255)                                                  gCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactgaagaac                                                                                        <  1:684416/151‑1 (MQ=255)                                                  gCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactgaagaac                                                                                        >  1:1470900/1‑151 (MQ=255)                                                   cTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGgaagaactgaagaact                                                                                       >  1:1042226/1‑151 (MQ=255)                                                     gtgGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACtt                                                                                      <  1:356328/150‑1 (MQ=255)                                                        gAACGATTTTCTTAAACATAGCCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGGCCTGCCGCTGGAAGAACTGAAGAACTTcc                                                                                    <  1:25139/149‑1 (MQ=255)                                                         aaCGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTc                                                                                 >  2:610771/1‑151 (MQ=255)                                                         aaCGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTc                                                                                 >  1:506850/1‑151 (MQ=255)                                                           cGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGAGCTGCCGCTGGAAGAACTGAAGAACTTCCGTc                                                                                 >  1:6202/1‑149 (MQ=255)                                                             aTTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGtt                                                                             <  1:768691/151‑1 (MQ=255)                                                                         aTAACCCTCCCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGaaaa                                                                  <  2:763258/150‑1 (MQ=255)                                                                         aTAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAAc                                                                 >  1:1037491/1‑151 (MQ=255)                                                                           aaCCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAAccc                                                               <  2:1148407/151‑1 (MQ=255)                                                                            aCCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAAcccc                                                              >  2:1236394/1‑151 (MQ=255)                                                                             cccTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTCCATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCa                                                             <  2:697075/151‑1 (MQ=255)                                                                             cccTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAAcccc                                                              >  2:1273713/1‑150 (MQ=255)                                                                              ccTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAAcccc                                                              >  2:689721/1‑149 (MQ=255)                                                                               cTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAgg                                                           >  1:426201/1‑151 (MQ=255)                                                                               cTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAgg                                                           >  2:215302/1‑151 (MQ=255)                                                                                 aCCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGcc                                                         >  1:304151/1‑151 (MQ=255)                                                                                 aCCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGcc                                                         >  1:1113033/1‑151 (MQ=255)                                                                                  ccGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGcc                                                         <  1:1332429/150‑1 (MQ=255)                                                                                   cGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCAc                                                       >  2:1027359/1‑151 (MQ=255)                                                                                    gACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCAcc                                                      >  2:791800/1‑151 (MQ=255)                                                                                       ccAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCgg                                                   >  2:53347/1‑151 (MQ=255)                                                                                       ccAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCgg                                                   <  1:746119/151‑1 (MQ=255)                                                                                              ggTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGAtt                                              <  2:1015592/149‑1 (MQ=255)                                                                                                 aTGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCt                                          >  1:390721/1‑150 (MQ=255)                                                                                                  tGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCtat                                        >  2:887649/1‑151 (MQ=255)                                                                                                      cgcgACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACg                                     <  2:1277634/150‑1 (MQ=255)                                                                                                       gcgACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCTCCCGGAGATTGGCTATACg                                     <  1:1043710/149‑1 (MQ=255)                                                                                                          aCCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAg                                 >  1:1064932/1‑150 (MQ=255)                                                                                                            cGCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCg                              >  2:309071/1‑151 (MQ=255)                                                                                                             gCTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGt                             <  2:70444/151‑1 (MQ=255)                                                                                                              cTTTATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGtt                            >  2:1159398/1‑151 (MQ=255)                                                                                                               tttATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTg                           >  1:1370701/1‑151 (MQ=255)                                                                                                                 tATTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGa                          <  2:718309/150‑1 (MQ=255)                                                                                                                  aTTCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGaa                         >  2:113609/1‑150 (MQ=255)                                                                                                                   ttCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGaaa                        <  1:606310/150‑1 (MQ=255)                                                                                                                   ttCTTTCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAac                       >  2:138008/1‑151 (MQ=255)                                                                                                                        tCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccacc                   <  1:109227/150‑1 (MQ=255)                                                                                                                        tCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccacc                   >  2:965405/1‑150 (MQ=255)                                                                                                                        tCCAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccacc                   >  1:300121/1‑150 (MQ=255)                                                                                                                         ccACCGGTCACGCGTCGATGCCGCGCTACAGTTTGCTACATCGGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAacc                      <  2:748214/146‑1 (MQ=255)                                                                                                                         ccAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccaccac                 <  1:1438208/151‑1 (MQ=255)                                                                                                                         ccAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccacca                  <  2:544550/150‑1 (MQ=255)                                                                                                                          cAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccaccacc                >  2:194241/1‑151 (MQ=255)                                                                                                                          cAACGGTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAaccaccacc                >  2:669582/1‑151 (MQ=255)                                                                                                                              ggTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGcc            >  2:730944/1‑151 (MQ=255)                                                                                                                               gTCACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGccc           <  2:1112934/151‑1 (MQ=255)                                                                                                                                 cACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGc         >  2:1172078/1‑151 (MQ=255)                                                                                                                                 cACGCGTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGc         >  2:834723/1‑151 (MQ=255)                                                                                                                                   cgcgTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGCCCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATCCGCCAGGCGTTGAACCCACCACCGGccc           <  1:737100/147‑1 (MQ=255)                                                                                                                                   cgcgTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCtt       <  2:789310/151‑1 (MQ=255)                                                                                                                                    gcgTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTg      >  1:1063029/1‑151 (MQ=255)                                                                                                                                     cgTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTgg     >  1:433152/1‑151 (MQ=255)                                                                                                                                      gTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTgg     >  1:381283/1‑150 (MQ=255)                                                                                                                                      gTCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGCCCCCCCGGAGATTGGCTATACGCCAGGCGTTGAAACCCCCGCCGGCCCGCTTGGa    <  2:957473/151‑1 (MQ=255)                                                                                                                                       tCGATGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGAc   >  2:139449/1‑151 (MQ=255)                                                                                                                                          aTGCCGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACa  >  2:383440/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                 ACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACA  >  NC_000913/2579700‑2579985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑