Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,647,150	G→A	A59V (GCT→GTT)	pbpC ←	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,647,150	0	G	A	100.0%	349.4 / NA	85	A59V (GCT→GTT)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/42);  total (43/42)

AGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCA  >  NC_000913/2647006‑2647295                                                                                                                                                 |                                                                                                                                                  agaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCgg                                                                                                                                             >  2:1468336/1‑151 (MQ=255)    tCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCg                                                                                                                                           >  2:1153539/1‑150 (MQ=255)       tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGaa                                                                                                                                         <  2:472681/149‑1 (MQ=255)       tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGaa                                                                                                                                         <  1:1195732/149‑1 (MQ=255)       tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGa                                                                                                                                          <  1:304556/148‑1 (MQ=255)            tgctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGcc                                                                                                                                    >  2:1426001/1‑149 (MQ=255)                gcgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAgcg                                                                                                                              <  2:1041907/151‑1 (MQ=255)                gcgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAgcg                                                                                                                              >  2:1343193/1‑151 (MQ=255)                 cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCagag                                                                                                                                <  2:964260/148‑1 (MQ=255)                      gcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTAc                                                                                                                        >  2:745418/1‑151 (MQ=255)                            gagaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCt                                                                                                                  <  2:772221/151‑1 (MQ=255)                            gagaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCt                                                                                                                  <  1:465581/151‑1 (MQ=255)                             agaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTg                                                                                                                 >  2:495038/1‑151 (MQ=255)                                  ggATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGcc                                                                                                             >  1:1298084/1‑150 (MQ=255)                                  ggATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCa                                                                                                            <  1:1450237/151‑1 (MQ=255)                                    aTTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACg                                                                                                          <  1:30817/151‑1 (MQ=255)                                         cccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCa                                                                                                      <  2:572143/150‑1 (MQ=255)                                         cccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCAc                                                                                                     <  2:249072/151‑1 (MQ=255)                                          ccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACt                                                                                                    >  1:1145489/1‑151 (MQ=255)                                               gATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGc                                                                                               <  1:867176/151‑1 (MQ=255)                                                aTGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCg                                                                                              >  2:699052/1‑151 (MQ=255)                                                      ccAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGAtt                                                                                         <  2:365466/150‑1 (MQ=255)                                                      ccAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTg                                                                                        >  2:433987/1‑151 (MQ=255)                                                        aGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGAc                                                                                      >  2:808282/1‑151 (MQ=255)                                                          aaCCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt                                                                                    <  1:664567/151‑1 (MQ=255)                                                                cGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGc                                                                               >  1:1239644/1‑150 (MQ=255)                                                                 gATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGc                                                                               >  2:138214/1‑149 (MQ=255)                                                                    cTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                          >  1:1384338/1‑151 (MQ=255)                                                                    cTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                          >  2:423764/1‑151 (MQ=255)                                                                      tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCa                                                                              <  2:996515/145‑1 (MQ=255)                                                                      tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                          <  1:446157/149‑1 (MQ=255)                                                                      tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCg                                                                           <  2:134430/148‑1 (MQ=255)                                                                          aaTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTagag                                                                     <  1:1186764/150‑1 (MQ=255)                                                                             tGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                  >  1:724036/1‑150 (MQ=255)                                                                              gATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                  >  2:1052710/1‑149 (MQ=255)                                                                                  aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                            <  1:617792/151‑1 (MQ=255)                                                                                  aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                            <  1:1153635/151‑1 (MQ=255)                                                                                       ttCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCt                                                       >  1:468909/1‑151 (MQ=255)                                                                                        tCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTg                                                      >  2:45566/1‑151 (MQ=255)                                                                                         cAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGc                                                     >  1:795872/1‑151 (MQ=255)                                                                                               aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTcccc                                                <  2:779807/150‑1 (MQ=255)                                                                                               aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                               <  1:1222758/151‑1 (MQ=255)                                                                                                  gTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGc                                            >  2:1245689/1‑151 (MQ=255)                                                                                                    ggAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTg                                          <  1:1176405/151‑1 (MQ=255)                                                                                                      agaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGc                                         >  2:1314978/1‑150 (MQ=255)                                                                                                       gaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                        >  2:1266372/1‑150 (MQ=255)                                                                                                        aaaCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAg                                      <  1:1128577/151‑1 (MQ=255)                                                                                                          aCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaa                                    <  1:1158443/151‑1 (MQ=255)                                                                                                          aCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGa                                     >  1:1021370/1‑150 (MQ=255)                                                                                                           cATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaa                                    >  1:1425639/1‑150 (MQ=255)                                                                                                            aTCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTCTATCTGCTCCCCACGCTGCAAGaaa                                   >  1:927908/1‑150 (MQ=255)                                                                                                               ttCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                               >  2:212133/1‑151 (MQ=255)                                                                                                                tCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaaaa                                  <  1:973716/147‑1 (MQ=255)                                                                                                                 cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                               >  2:1075639/1‑133 (MQ=255)                                                                                                                 cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                               <  1:1075740/133‑1 (MQ=255)                                                                                                                 cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                               >  1:246718/1‑149 (MQ=255)                                                                                                                  gATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGgaga                            <  2:298297/151‑1 (MQ=255)                                                                                                                   aTTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGaa                           <  2:351925/151‑1 (MQ=255)                                                                                                                     tGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGaa                           >  1:872100/1‑149 (MQ=255)                                                                                                                      gTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAgg                        >  1:1136693/1‑151 (MQ=255)                                                                                                                       ttACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAggg                       >  1:1246603/1‑151 (MQ=255)                                                                                                                        tACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAggg                       <  1:423820/150‑1 (MQ=255)                                                                                                                         aCCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcg                     <  1:1306183/151‑1 (MQ=255)                                                                                                                         aCCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgc                      <  1:620578/150‑1 (MQ=255)                                                                                                                          ccGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcgc                    <  1:188248/151‑1 (MQ=255)                                                                                                                          ccGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcgc                    >  1:837135/1‑151 (MQ=255)                                                                                                                          ccGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcgc                    >  2:708235/1‑151 (MQ=255)                                                                                                                           cGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcg                     >  2:957966/1‑149 (MQ=255)                                                                                                                           cGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGcgcg                   >  2:1174739/1‑151 (MQ=255)                                                                                                                            ggATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCgg                  <  2:365722/151‑1 (MQ=255)                                                                                                                            ggATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCgg                  >  1:515669/1‑151 (MQ=255)                                                                                                                              aTAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCt                <  2:152679/151‑1 (MQ=255)                                                                                                                              aTAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCt                <  1:745496/151‑1 (MQ=255)                                                                                                                                aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc              >  2:1138450/1‑151 (MQ=255)                                                                                                                                 aCGCCAGATGCCGTCAACATCGGCGAAGCGGCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGcc             >  2:233619/1‑151 (MQ=255)                                                                                                                                    ccAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAAc          >  1:886666/1‑151 (MQ=255)                                                                                                                                      aGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGt        <  1:108426/151‑1 (MQ=255)                                                                                                                                       gATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGtt       <  1:915509/151‑1 (MQ=255)                                                                                                                                       gATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGtt       <  2:1297979/151‑1 (MQ=255)                                                                                                                                         tGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTAt     >  2:553477/1‑151 (MQ=255)                                                                                                                                          gCCGTTAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATc    >  2:571194/1‑151 (MQ=255)                                                                                                                                          gCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTAt     <  2:395562/150‑1 (MQ=255)                                                                                                                                          gCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATc    <  1:1052805/151‑1 (MQ=255)                                                                                                                                          gCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATc    >  2:1261654/1‑151 (MQ=255)                                                                                                                                            cGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCca  <  2:318397/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCA  >  NC_000913/2647006‑2647295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑