Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,909 T→C K1460R (AAA→AGA)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,9090TC100.0% 381.0 / NA 103K1460R (AAA→AGA) yfhMbacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/45);  total (58/45)

Only 100 of 105 total aligned reads displayed.

GCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTTTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGT  >  NC_000913/2647763‑2648056
                                                                                                                                                  |                                                                                                                                                   
gCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCtctctc                                                                                                                                                 <  2:249877/151‑1 (MQ=255)
gCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCtctctc                                                                                                                                                 <  1:1111311/151‑1 (MQ=255)
 cTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCg                                                                                                                                                >  1:884274/1‑151 (MQ=255)
  tCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCTCTCTCTCGc                                                                                                                                               >  1:481112/1‑151 (MQ=255)
   cGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCg                                                                                                                                                <  1:1241867/149‑1 (MQ=255)
   cGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGcc                                                                                                                                              >  1:241439/1‑151 (MQ=255)
   cGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGc                                                                                                                                               <  1:396528/150‑1 (MQ=255)
    gATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCt                                                                                                                                             >  1:38329/1‑151 (MQ=255)
       ttGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTc                                                                                                                                          >  2:638621/1‑151 (MQ=255)
        tGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTc                                                                                                                                          >  2:576732/1‑150 (MQ=255)
          cagcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgc                                                                                                                                       >  2:694446/1‑151 (MQ=255)
          cagcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgc                                                                                                                                       >  1:45786/1‑151 (MQ=255)
           agcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgct                                                                                                                                      <  1:1405878/151‑1 (MQ=255)
           agcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgct                                                                                                                                      >  1:1409986/1‑151 (MQ=255)
           agcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGAGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgct                                                                                                                                      >  1:846716/1‑151 (MQ=255)
            gcaCATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgctg                                                                                                                                     <  2:699716/151‑1 (MQ=255)
             cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgctgc                                                                                                                                    <  2:552040/151‑1 (MQ=255)
              acaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAgctgct                                                                                                                                   >  2:479236/1‑151 (MQ=255)
               caTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTc                                                                                                                                  <  1:275471/151‑1 (MQ=255)
                aTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCa                                                                                                                                 <  1:1216232/151‑1 (MQ=255)
                  tGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAg                                                                                                                                <  2:1296112/150‑1 (MQ=255)
                   gTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGc                                                                                                                               <  1:1251974/150‑1 (MQ=255)
                     tCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTg                                                                                                                             <  1:86898/150‑1 (MQ=255)
                        ccGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTgaga                                                                                                                          >  1:665733/1‑150 (MQ=255)
                         cGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGaa                                                                                                                         >  2:408841/1‑150 (MQ=255)
                          ggTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGaa                                                                                                                         >  2:173838/1‑149 (MQ=255)
                          ggTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAag                                                                                                                       <  2:525309/151‑1 (MQ=255)
                            tAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAag                                                                                                                       >  1:1325420/1‑149 (MQ=255)
                                  tgcggattgcggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTgc                                                                                                               >  1:1087461/1‑151 (MQ=255)
                                    cggattgcggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTgcgc                                                                                                             >  2:1210234/1‑151 (MQ=255)
                                        ttgcggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGc                                                                                                         >  1:285171/1‑151 (MQ=255)
                                         tgcggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGGGACAAGTTGATCACTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGcc                                                                                                        >  1:354740/1‑151 (MQ=255)
                                           cggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCAt                                                                                                      <  1:647556/151‑1 (MQ=255)
                                           cggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCAt                                                                                                      >  2:617536/1‑151 (MQ=255)
                                            ggatAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCAtt                                                                                                     >  1:1325864/1‑151 (MQ=255)
                                                 aCCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCg                                                                                                <  1:230997/151‑1 (MQ=255)
                                                 aCCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCg                                                                                                >  1:146453/1‑151 (MQ=255)
                                                 aCCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCg                                                                                                <  2:849450/151‑1 (MQ=255)
                                                   cGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCggg                                                                                              >  2:432363/1‑151 (MQ=255)
                                                        gCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAAt                                                                                         >  2:230728/1‑151 (MQ=255)
                                                         cATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATc                                                                                        <  1:78202/151‑1 (MQ=255)
                                                         cATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATc                                                                                        >  1:868961/1‑151 (MQ=255)
                                                            ccATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTg                                                                                     <  2:201100/151‑1 (MQ=255)
                                                               tACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAAt                                                                                  >  1:1292354/1‑151 (MQ=255)
                                                                aCGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAAt                                                                                  <  1:1234292/150‑1 (MQ=255)
                                                                aCGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAAt                                                                                  <  1:849307/150‑1 (MQ=255)
                                                                aCGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATc                                                                                 >  2:1000777/1‑151 (MQ=255)
                                                                aCGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATc                                                                                 <  1:282491/151‑1 (MQ=255)
                                                                 cGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCg                                                                                >  2:55045/1‑151 (MQ=255)
                                                                 cGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCg                                                                                <  1:891429/151‑1 (MQ=255)
                                                                   cAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCgca                                                                              <  2:589961/151‑3 (MQ=255)
                                                                   cAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCgc                                                                               >  2:557838/1‑149 (MQ=255)
                                                                   cAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCgc                                                                               >  1:785733/1‑149 (MQ=255)
                                                                    aaCCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCAc                                                                             >  1:858392/1‑151 (MQ=255)
                                                                       cATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACggg                                                                          <  2:224970/151‑1 (MQ=255)
                                                                          aaCGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGc                                                                         >  2:60911/1‑149 (MQ=255)
                                                                            cGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGcc                                                                     >  1:956455/1‑151 (MQ=255)
                                                                               cTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAgg                                                                  >  2:684258/1‑151 (MQ=255)
                                                                                tGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAgg                                                                  >  2:880359/1‑150 (MQ=255)
                                                                                tGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAgg                                                                  >  2:340406/1‑150 (MQ=255)
                                                                                    cGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGaaca                                                              <  1:1003298/150‑1 (MQ=255)
                                                                                    cGCCACTGTTGCTCACCTGCAAGGTCACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGaaca                                                              <  1:347832/150‑1 (MQ=255)
                                                                                        aCTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAAcgcg                                                         >  2:1366311/1‑151 (MQ=255)
                                                                                                 cACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACttt                                                  <  1:829693/149‑1 (MQ=255)
                                                                                                   ccTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTg                                              >  1:1384131/1‑151 (MQ=255)
                                                                                                   ccTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTg                                              <  1:609781/151‑1 (MQ=255)
                                                                                                   ccTGCAAGGTGACAAGTTGATCACTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTg                                              <  2:354693/151‑1 (MQ=255)
                                                                                                      gCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGc                                             <  2:611710/149‑1 (MQ=255)
                                                                                                       cAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCgac                                          <  2:1129983/151‑3 (MQ=255)
                                                                                                       cAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTc                                          <  2:90732/151‑1 (MQ=255)
                                                                                                       cAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTc                                          <  1:442838/151‑1 (MQ=255)
                                                                                                        aaGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCg                                         >  2:283497/1‑151 (MQ=255)
                                                                                                        aaGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCg                                         >  2:157326/1‑151 (MQ=255)
                                                                                                         aGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGa                                        <  1:986355/151‑1 (MQ=255)
                                                                                                           gTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGAt                                       >  1:96882/1‑150 (MQ=255)
                                                                                                               cAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGcc                                   >  2:371134/1‑150 (MQ=255)
                                                                                                                aaGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGcc                                   >  2:722446/1‑149 (MQ=255)
                                                                                                                aaGTTGATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGcc                                   >  2:591409/1‑149 (MQ=255)
                                                                                                                     gATCGCTATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGtt                             <  2:1351445/150‑1 (MQ=255)
                                                                                                                           tATTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCaa                       >  1:147525/1‑150 (MQ=255)
                                                                                                                            aTTCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAAc                     <  1:459958/151‑1 (MQ=255)
                                                                                                                             ttCAGATTGCTGTTCAGCGCTCTCTCGCCTGTCGGCTCCTCAGCTGAGAAAGCGGTTTGCGCCTGCCATTTACCGGGCAACTCCTGACTCGCACGGGCAGCCAGCAACGACGCGTTACTTTCCTGCGTTGATACCCAGCGTTCACCaaa                      >  2:1276383/1‑149 (MQ=255)
                                                                                                                              tCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACg                    <  1:84146/150‑1 (MQ=255)
                                                                                                                              tCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACg                    <  1:32080/150‑1 (MQ=255)
                                                                                                                              tCAGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACg                    <  1:230751/150‑1 (MQ=255)
                                                                                                                                aGATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCct                 >  2:137764/1‑151 (MQ=255)
                                                                                                                                 gATTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCct                 <  1:1188124/150‑1 (MQ=255)
                                                                                                                                  aTTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctgc               <  2:124221/151‑1 (MQ=255)
                                                                                                                                  aTTGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctgc               >  1:288057/1‑151 (MQ=255)
                                                                                                                                   ttGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGATTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctgct              >  1:1219176/1‑151 (MQ=255)
                                                                                                                                    tGCTGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctgctg             >  1:1085931/1‑151 (MQ=255)
                                                                                                                                       tGTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCg           >  1:238155/1‑150 (MQ=255)
                                                                                                                                        gTTTTGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGa          <  1:213230/150‑1 (MQ=255)
                                                                                                                                          tttGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGaa         >  2:242579/1‑149 (MQ=255)
                                                                                                                                           ttGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgt      >  2:509210/1‑151 (MQ=255)
                                                                                                                                            tGCGCTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgt      <  1:694524/150‑1 (MQ=255)
                                                                                                                                             gcgcTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgtgt    <  1:432419/151‑1 (MQ=255)
                                                                                                                                              cgcTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgtgtg   <  1:1119720/151‑1 (MQ=255)
                                                                                                                                              cgcTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgtgtg   >  1:1072474/1‑151 (MQ=255)
                                                                                                                                               gcTCTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGCACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgcgtgt  >  1:188319/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCTGCAAGGTGACAAGTTGATCGCTATTCAGATTGCTGTTTTGCGCTTTCTCGCCTGTCAGCTGCTCAGCTGAGAAAGAGGTTTGCGCCTGCCATTTACCGGGTAAATCCTGAATCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGT  >  NC_000913/2647763‑2648056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑