Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 42.6% | 62.0 / 42.4 | 61 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (2/33); new base T (26/0); total (28/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.10e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACA > NC_000913/3206291‑3206578 | aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACa < 1:476944/151‑1 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACaaa > 2:405793/1‑150 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGAGAATTTTTTCCGAaaaa > 1:775432/1‑150 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACaaa < 1:337242/149‑1 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATa < 2:1242518/151‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCCGACAAATa > 2:34307/1‑149 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCATACcaaaaaa > 1:901992/1‑146 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCACACAAAAACa > 2:701686/1‑150 (MQ=255) aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTTAGAAAAAAACaaaaa > 1:368567/1‑149 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAAATTTTTTAGACCAAAAAAAAAAATgggg > 1:99311/1‑149 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGc < 1:636919/150‑1 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGGGAATTTTTTCAGACAAATACAAAAAATGGAGt > 1:914589/1‑147 (MQ=255) ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa < 2:121361/151‑1 (MQ=255) cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTTAGACAAAAACAAAAAATGGGGTTGCCat > 1:312428/1‑151 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAAGTTTTTTAGGCAAATATaaaaattaaaggtgccct > 2:948686/1‑137 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTTATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga < 1:513202/151‑1 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga < 2:168845/151‑1 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga < 2:384521/149‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTTAGACAAAAACAAAAAAAGGAGTTGGCATGATGAgggga > 1:729050/1‑146 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTTAATTTTTTTACAAAAAAACAAAAAATAGAGGTGGCATGATGAg > 1:829021/1‑145 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta < 2:61771/150‑1 (MQ=255) cattcTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACACTGATAGTGGT‑AGTTTGTTGCTGTTAATTTTTCCAGACGAATACAAAAACTTGACTTGCCAGGATGAGCGAAAGcaata < 1:438922/150‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGAGAAATTTTTCAGAAAAAAACAAAAACTGGAGTTGCCATCATTAGCGAAAGgaaaaa > 2:779954/1‑150 (MQ=255) ttcTAATTTCCGGG‑TGAATCAAGAACTGGTTATTACATAATCCCGGTTGCAAAAAAAATAAAACCCCAATTTTAGTTGTAAGTTTG‑GGGGGTTAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata < 2:582431/147‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa < 2:1184417/149‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa < 2:242554/148‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 1:36749/150‑1 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga < 2:576706/130‑1 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 2:1183091/151‑1 (MQ=255) tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt < 2:507923/150‑1 (MQ=255) cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAAGGAGTTGCCATGATGAGGGAAAGGAATAAGAAACAAGGCGTGaa > 2:552937/1‑142 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa < 1:974127/151‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag < 2:1111426/151‑1 (MQ=255) cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATTGAGTTGCCATGATGAGCGAAAGAAATAAAAAAAAGGCAGTGAATAAATTGACag > 2:336074/1‑149 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:70680/149‑1 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt < 1:140916/150‑1 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg < 2:977936/150‑1 (MQ=255) aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATAGGGTTGGCAATGTGGGCGAAAGAAATAAGGAACATGGAGTGAAAAAATTGACAGAGAATGtt > 2:481176/1‑150 (MQ=255) aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt < 2:665709/151‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa < 2:112535/151‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa < 1:24231/151‑1 (MQ=255) aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCCTGATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAATTGAAAGAGATTTTCGCTAACttt > 2:468068/1‑149 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGCGAATTTTTTCAGAAAAAAAAAAAAAAATGAGGTGGCCAGAAGAGCGGAAATAAAAAAAAAAAGGGAGGGGATAAGATGACAGAGAATGTTGCCaaaatt > 1:314787/1‑144 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGGAATTTTTCAGACAAAAACAAAAACAGGGGGTGTCACGCAGTGCGAAAGCAAAAAAAAACAAGGCGTGAAAAAGGTGGCAGAGAATGGTGCTAACTTTTACGcc > 2:1086829/1‑149 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATTGAGTTGCCATGATGAGCGGAAAGAAAAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTTCcccc > 2:288855/1‑148 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTAAATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt < 1:279305/151‑1 (MQ=255) tataAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAATGGAGGTGGCCTGATGAGGGGAAGGAATAAACAACAAGCAGGGAATAAATTGACAGAGATTGTCGCTAACTTTAACCCCCTGAttttt > 2:638958/1‑148 (MQ=255) aaaaacCCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCAAGACAAATACAAAAACTGGAGTTGCATTGATGGGAAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGCAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTAc < 1:701652/149‑1 (MQ=255) aaaaCCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAAATGGAGGTGCCCTGATGAGCGGAAATAAAAAACAACAAGCAGGGAATAAAGTGACAGAGATTTTTGCTAAATTTACCGCCATGATTTCTTccc > 2:274359/1‑151 (MQ=255) aaaCCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCg < 1:627120/151‑1 (MQ=255) aaCCACATTTTTAGTGGT‑AGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa < 2:241917/151‑1 (MQ=255) tttttAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAAGGAGTTGGCCTGATGAGCGAAAGGAATAAACAAAAAGCAGTGAATAAATTTACAGAGATTGTCGCTAACTTTATCCCCCTGATTTTTTCCCGAATGCCt > 2:384841/1‑150 (MQ=255) tttttAGTGGT‑AGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTTGGGGTGCCCTGATGAGCGGAAAAAATAAACAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTAACGCCCTGATTTTTACCCCAATGc > 2:149835/1‑148 (MQ=255) ttttAGTGGT‑AGTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAACCTGGAGTTGCCATGATGGGCAAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCtg < 2:56573/150‑1 (MQ=255) tttAGTGGT‑AGTTTGTGGCGGGGGATTTTTTCCGACAAAAAAAAAAAATGGAGTTGGCATGAGGAGCGAAAGTAAAAAACAACAGGGAGGGAATAAGTTGACAGAGATTGTTGCTAACTTTATCGCCATGATTTTTTCCCGAAAGcc > 1:891781/1‑147 (MQ=255) aGTGGT‑AGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAAAGGAGTTGCCATGATGAGCGGAAGGAATAAACAACAGGCAGGGAAAAAATTGACAGAGATTTTGGCTAACTTTACCCCCATGATTTTTACCCCAAAGcccg > 2:1093529/1‑144 (MQ=255) ttggtgGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCAAAAGAAATAAGCAACAGGCAGTAAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg < 1:580320/146‑1 (MQ=255) ggCGGTAATTTTTCCCAAAAAAATACAAAAACTGGAGTTCCCATGATGAGGAAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa < 1:1172109/150‑1 (MQ=255) tGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa > 2:861014/1‑150 (MQ=255) aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc > 1:448342/1‑150 (MQ=255) tttttCCAGCCAAATAAAAAACCGGGAGTTGCCAGGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGCGATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa < 2:88488/149‑1 (MQ=255) | ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGT‑AGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACA > NC_000913/3206291‑3206578 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |