Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 96,085 C→T V28V (GTC→GTT mraY → phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091396,0850CT98.6% 274.2 / NA 70V28V (GTC→GTTmraYphospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (44/25);  minor base G (1/0);  total (45/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATT  >  NC_000913/95938‑96232
                                                                                                                                                   |                                                                                                                                                   
tAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAg                                                                                                                                                   <  2:874939/150‑1 (MQ=255)
 aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGcc                                                                                                                                                 <  1:533611/151‑1 (MQ=255)
 aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGcc                                                                                                                                                 >  2:864388/1‑151 (MQ=255)
  gTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCct                                                                                                                                                <  2:57386/151‑1 (MQ=255)
   ttAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCTGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCctg                                                                                                                                               >  1:14956/1‑151 (MQ=255)
     aaGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCctgct                                                                                                                                             >  1:617676/1‑151 (MQ=255)
      aGGGTGCACGTAGTGCCGCAAGGGAAGAGGGAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCctgctg                                                                                                                                            >  1:378308/1‑151 (MQ=255)
        ggTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGAc                                                                                                                                          >  2:923066/1‑151 (MQ=255)
            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTATAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACcgcg                                                                                                                                      >  2:571663/1‑151 (MQ=255)
            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACcgcg                                                                                                                                      >  1:1155022/1‑151 (MQ=255)
             aCGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCgcgc                                                                                                                                     >  1:1190942/1‑151 (MQ=255)
             aCGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCgcgc                                                                                                                                     >  1:829429/1‑151 (MQ=255)
              cGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCt                                                                                                                                    >  2:232646/1‑151 (MQ=255)
              cGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCt                                                                                                                                    >  1:857880/1‑151 (MQ=255)
                          aTGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCAtt                                                                                                                        <  1:752339/151‑1 (MQ=255)
                               agagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTgg                                                                                                                    >  2:908787/1‑150 (MQ=255)
                               agagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTgg                                                                                                                    >  1:657123/1‑150 (MQ=255)
                                 agGTAGGACGCGCTTTACAGGAGAATGGGACATGGTAGGTTGGCTGGCCGAACATTTGGTCAAATATTATGCCGGCTTTAAGGTCTTTTCCTATCTGACGTTTCGCGCCATCGTGAGCCTGCTGACCGCGCTGTTCATCTCATtgtg                                                                                                                     >  1:368470/1‑147 (MQ=255)
                                   gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATggg                                                                                                               >  2:613297/1‑151 (MQ=255)
                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                                                                                                              <  1:349500/150‑1 (MQ=255)
                                        aCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCcg                                                                                                           <  2:608908/150‑1 (MQ=255)
                                         cgcgCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCcgcg                                                                                                         >  1:314707/1‑151 (MQ=255)
                                             cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTAt                                                                                                      >  1:731396/1‑150 (MQ=255)
                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg                                                                                                     <  2:299060/149‑1 (MQ=255)
                                                   aGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCt                                                                                               >  2:500328/1‑151 (MQ=255)
                                                    ggAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTc                                                                                              >  2:1244739/1‑151 (MQ=255)
                                                      agaATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAt                                                                                            >  1:477250/1‑151 (MQ=255)
                                                       gaATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt                                                                                           <  1:427317/151‑1 (MQ=255)
                                                         aTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCTAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTg                                                                                         >  2:1204133/1‑151 (MQ=255)
                                                         aTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTg                                                                                         >  1:1162198/1‑151 (MQ=255)
                                                                    tAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACttt                                                                               <  2:333483/150‑1 (MQ=255)
                                                                    tAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTc                                                                              >  2:976217/1‑151 (MQ=255)
                                                                          ggctggcCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTgg                                                                        <  2:150309/151‑1 (MQ=255)
                                                                              ggcCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCAGCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCa                                                                     <  1:693715/150‑1 (MQ=255)
                                                                               gcCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAgg                                                                   >  2:181433/1‑151 (MQ=255)
                                                                               gcCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAgg                                                                   >  1:329035/1‑151 (MQ=255)
                                                                                 cGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAggtg                                                                 <  2:238584/151‑1 (MQ=255)
                                                                                   aaCATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAggtg                                                                 >  1:1006101/1‑149 (MQ=255)
                                                                                    aCATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGgtggtg                                                              <  2:683231/151‑1 (MQ=255)
                                                                                    aCATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGgtggtg                                                              >  2:235846/1‑151 (MQ=255)
                                                                                      aTTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCg                                                            >  2:959463/1‑151 (MQ=255)
                                                                                       tttGGGCAACTATTCGTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCg                                                            >  1:882919/1‑150 (MQ=255)
                                                                                        ttGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGt                                                           >  2:822958/1‑150 (MQ=255)
                                                                                          ggTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTaa                                                         <  1:554436/150‑1 (MQ=255)
                                                                                          cgTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAac                                                        >  1:164131/2‑151 (MQ=255)
                                                                                            tCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAacg                                                       >  2:507230/1‑150 (MQ=255)
                                                                                             cAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAacgac                                                     >  1:19272/1‑151 (MQ=255)
                                                                                             cAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAacgac                                                     >  1:1178820/1‑151 (MQ=255)
                                                                                             cAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAacga                                                      <  1:216179/150‑1 (MQ=255)
                                                                                                atatTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGt                                                  >  2:1284025/1‑151 (MQ=255)
                                                                                                atatTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGt                                                  >  1:467373/1‑151 (MQ=255)
                                                                                                atatTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGt                                                  >  1:304047/1‑151 (MQ=255)
                                                                                                      ttCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGa                                             >  2:1165919/1‑150 (MQ=255)
                                                                                                       tCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGaa                                            <  1:1106994/150‑1 (MQ=255)
                                                                                                        ccGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATc                                          >  1:769705/1‑151 (MQ=255)
                                                                                                         cGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATca                                         <  2:504615/151‑1 (MQ=255)
                                                                                                            cTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCacac                                      >  1:869237/1‑151 (MQ=255)
                                                                                                                aaCGTCTTTTCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTc                                   >  1:255913/1‑150 (MQ=255)
                                                                                                                       tttCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCaa                              <  2:14951/148‑1 (MQ=255)
                                                                                                                        ttCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAgcgc                          >  2:458289/1‑151 (MQ=255)
                                                                                                                         tCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAgcgc                          <  2:106989/150‑1 (MQ=255)
                                                                                                                         tCCTATCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAgcgc                          <  2:938983/150‑1 (MQ=255)
                                                                                                                             aTCTGACGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTa                      <  1:387023/150‑1 (MQ=255)
                                                                                                                                   cGTTTCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGAc               <  1:26628/151‑1 (MQ=255)
                                                                                                                                     tttCGCGCCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGAcc              >  1:851630/1‑150 (MQ=255)
                                                                                                                                           gcCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCg       >  2:558182/1‑151 (MQ=255)
                                                                                                                                           gcCATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCg       <  2:585789/151‑1 (MQ=255)
                                                                                                                                             cATCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCggg     <  2:479346/151‑1 (MQ=255)
                                                                                                                                               tCGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGAt   >  1:733594/1‑151 (MQ=255)
                                                                                                                                                cGTTAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGAtt  <  1:882647/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATT  >  NC_000913/95938‑96232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: