Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,612,540	T→C	W226R (TGG→CGG)	yhhT →	UPF0118 family putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,612,540	0	T	C	100.0%	275.7 / NA	75	W226R (TGG→CGG)	yhhT	UPF0118 family putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/27);  total (48/27)

ATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGT  >  NC_000913/3612394‑3612685                                                                                                                                                   |                                                                                                                                                  ataatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCggg                                                                                                                                                <  1:288121/150‑1 (MQ=255)  taatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGc                                                                                                                                               >  1:1150600/1‑150 (MQ=255)       cacaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACt                                                                                                                                         >  2:1194070/1‑151 (MQ=255)            aTTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGt                                                                                                                                    >  2:850462/1‑151 (MQ=255)            aTTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGt                                                                                                                                    <  1:224850/151‑1 (MQ=255)               cacaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGttt                                                                                                                                  <  2:566013/150‑1 (MQ=255)                  aTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGc                                                                                                                              >  1:920012/1‑151 (MQ=255)                  aTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGc                                                                                                                              >  2:442656/1‑151 (MQ=255)                   tCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGc                                                                                                                              >  1:508351/1‑150 (MQ=255)                     gcgGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTc                                                                                                                            >  2:689797/1‑150 (MQ=255)                     gcgGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCa                                                                                                                           <  2:4721/151‑1 (MQ=255)                        ggATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACt                                                                                                                        <  1:881148/151‑1 (MQ=255)                            tACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGt                                                                                                                    >  1:595474/1‑151 (MQ=255)                             acacCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTGAACTACGTg                                                                                                                   <  2:490993/151‑1 (MQ=255)                              cacCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGc                                                                                                                  >  2:330424/1‑151 (MQ=255)                              cacCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGc                                                                                                                  >  2:1205112/1‑151 (MQ=255)                               acCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGcc                                                                                                                 >  1:1224033/1‑151 (MQ=255)                                    gcACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAat                                                                                                             >  1:1239653/1‑150 (MQ=255)                                          aaaGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGgcg                                                                                                      >  1:113171/1‑151 (MQ=255)                                            aGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGCGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGcgcg                                                                                                    >  1:729119/1‑151 (MQ=255)                                             ggCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCgg                                                                                                   >  2:613173/1‑151 (MQ=255)                                                gTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTa                                                                                                 >  1:21988/1‑150 (MQ=255)                                                    cGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAttt                                                                                             <  2:373782/150‑1 (MQ=255)                                                    cGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAttt                                                                                             <  1:642963/150‑1 (MQ=255)                                                    cGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTc                                                                                            >  2:1254309/1‑151 (MQ=255)                                                     gCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTcc                                                                                           >  2:737998/1‑151 (MQ=255)                                                     gCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTcc                                                                                           >  1:1054233/1‑151 (MQ=255)                                                      cACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccg                                                                                          >  2:764772/1‑151 (MQ=255)                                                      cACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTcc                                                                                           >  1:564995/1‑150 (MQ=255)                                                       aCTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgc                                                                                         >  2:1026338/1‑151 (MQ=255)                                                         tATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgccg                                                                                       >  2:386639/1‑151 (MQ=255)                                                          aTCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgccg                                                                                       <  2:43041/150‑1 (MQ=255)                                                          aTCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGt                                                                                      >  1:148033/1‑151 (MQ=255)                                                                     aaGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATg                                                                            >  2:865854/1‑150 (MQ=255)                                                                     aaGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATg                                                                            >  2:233617/1‑150 (MQ=255)                                                                       gACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAt                                                                          <  1:210482/150‑1 (MQ=255)                                                                        aCGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTc                                                                        >  1:128262/1‑151 (MQ=255)                                                                        aCGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTc                                                                        >  2:187673/1‑151 (MQ=255)                                                                        aCGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGAAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTAGGTGCCCAATATCGGCGCGGTAATTTCCGCCGGACCGCCAATggt                                                                          >  1:1218752/1‑147 (MQ=255)                                                                         cGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTc                                                                        >  2:300760/1‑150 (MQ=255)                                                                            tACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGt                                                                    >  2:105515/1‑151 (MQ=255)                                                                              cTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAgggg                                                                   >  1:1187975/1‑148 (MQ=255)                                                                              cTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAgg                                                                     <  1:882010/148‑1 (MQ=255)                                                                                 aGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtgctg                                                                >  2:34148/1‑150 (MQ=255)                                                                                  gTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTgctgct                                                              <  1:1234850/151‑1 (MQ=255)                                                                                    ttATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGctgctg                                                             <  2:710168/150‑1 (MQ=255)                                                                                     tATGGACAGGTGTCATCGTCGGGCTGGGGTTGGAGCTGATGGGGGTGCAATTTGCGCTGCTGCGGGCAGTACTGGCGTTTTTCCTCAACTACGTGCCCAGTATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGt                                                            <  1:43844/150‑1 (MQ=255)                                                                                      aTGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGtt                                                           <  2:776322/150‑1 (MQ=255)                                                                                        ggACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTa                                                         <  2:968354/150‑1 (MQ=255)                                                                                                 tCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGttt                                                >  1:400531/1‑150 (MQ=255)                                                                                                      gTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGAGCAGTACTGGCGTTTTTGCTCAACTGCGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAAt                                          <  1:36518/151‑1 (MQ=255)                                                                                                       tCTGGCTGGGGCTGGGGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGa                                            <  1:683566/148‑1 (MQ=255)                                                                                                        ctggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGt                                        >  2:443515/1‑151 (MQ=255)                                                                                                        ctggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGt                                        >  2:1130319/1‑151 (MQ=255)                                                                                                          ggctggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTAt                                      >  2:685872/1‑151 (MQ=255)                                                                                                              ggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTg                                  <  1:795214/151‑1 (MQ=255)                                                                                                                  ctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCg                              >  2:150050/1‑151 (MQ=255)                                                                                                                    ggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCgg                             <  2:657903/150‑1 (MQ=255)                                                                                                                     gAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGgcg                           >  1:1004522/1‑151 (MQ=255)                                                                                                                     gAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGgcg                           >  2:122951/1‑151 (MQ=255)                                                                                                                       gCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGgcgc                          >  1:541737/1‑150 (MQ=255)                                                                                                                          gATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTg                      >  2:45380/1‑151 (MQ=255)                                                                                                                           aTGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGt                     >  2:364319/1‑151 (MQ=255)                                                                                                                            tGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGtt                    >  2:997117/1‑151 (MQ=255)                                                                                                                             gggggTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGttt                   <  1:1194036/151‑1 (MQ=255)                                                                                                                               gggTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGttttt                 <  1:188454/151‑1 (MQ=255)                                                                                                                                     aGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCATCTACGTGCCCACTATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGt           <  2:63602/151‑1 (MQ=255)                                                                                                                                     aGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGt           <  2:14428/151‑1 (MQ=255)                                                                                                                                       tttGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTcc         >  1:812587/1‑151 (MQ=255)                                                                                                                                        ttGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTcc         >  1:270319/1‑150 (MQ=255)                                                                                                                                         tGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCa        <  1:1181673/150‑1 (MQ=255)                                                                                                                                         tGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCa        <  1:1143540/150‑1 (MQ=255)                                                                                                                                            gcTGATGCGGGTAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCatat     <  1:393192/150‑1 (MQ=255)                                                                                                                                              tGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGt  >  2:312235/1‑151 (MQ=255)                                                                                                                                              tGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGt  >  1:913474/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGT  >  NC_000913/3612394‑3612685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑