Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,784,753 T→C E88G (GAG→GGG)  yibN ← putative rhodanese‑related sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,784,7530TC98.8% 282.3 / ‑4.2 80E88G (GAG→GGG) yibNputative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (35/44);  total (35/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

TTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCTCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATC  >  NC_000913/3784610‑3784899
                                                                                                                                               |                                                                                                                                                  
tttCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAAc                                                                                                                                             >  1:599164/1‑151 (MQ=255)
   cgcccgccCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAt                                                                                                                                           >  1:7171/1‑150 (MQ=255)
   cGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAtt                                                                                                                                          >  2:1196630/1‑151 (MQ=255)
   cGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAtt                                                                                                                                          <  1:634724/151‑1 (MQ=255)
     ccagcccagcCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCTCACCAACAttgt                                                                                                                                        <  1:817796/151‑1 (MQ=255)
     ccagcccagcCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAttgt                                                                                                                                        <  1:102845/151‑1 (MQ=255)
      agagcctgccagcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAttgtt                                                                                                                                       >  1:1037641/8‑151 (MQ=255)
       agcccagccAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAGACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAttgttg                                                                                                                                      >  2:133209/1‑151 (MQ=255)
       agcccagccAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACAttgttg                                                                                                                                      <  2:1202987/151‑1 (MQ=255)
         cccagccAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGc                                                                                                                                    <  2:985959/151‑1 (MQ=255)
           cagccagcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCtt                                                                                                                                  <  2:1201804/151‑1 (MQ=255)
            agccagcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCttt                                                                                                                                 <  1:540021/151‑1 (MQ=255)
            agccagcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCttt                                                                                                                                 >  1:1122277/1‑151 (MQ=255)
            agccagcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCttt                                                                                                                                 <  2:1030269/151‑1 (MQ=255)
                agcGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGAtt                                                                                                                             <  2:400436/151‑1 (MQ=255)
                  cGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTc                                                                                                                           <  1:165442/151‑1 (MQ=255)
                   gACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTc                                                                                                                           >  1:1049586/1‑150 (MQ=255)
                   gACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCg                                                                                                                          >  1:505143/1‑151 (MQ=255)
                   gACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCg                                                                                                                          >  2:592366/1‑151 (MQ=255)
                   gACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCg                                                                                                                          <  1:1200522/151‑1 (MQ=255)
                       ccTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCg                                                                                                                      >  2:529103/1‑151 (MQ=255)
                       ccTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCg                                                                                                                      <  2:934335/151‑1 (MQ=255)
                          tcttTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCgg                                                                                                                     <  2:13955/149‑1 (MQ=255)
                          tcttTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCgg                                                                                                                     <  2:772586/149‑1 (MQ=255)
                           cttTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCa                                                                                                                   >  1:579428/1‑150 (MQ=255)
                            tttCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAAc                                                                                                                 >  2:871620/1‑151 (MQ=255)
                               cAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAAc                                                                                                                 >  2:301663/1‑148 (MQ=255)
                               cAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTACGCTCGGCAACAg                                                                                                               <  2:96539/150‑1 (MQ=255)
                                   aCGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTa                                                                                                           >  1:593338/1‑150 (MQ=255)
                                    cGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTaa                                                                                                          <  1:5770/150‑1 (MQ=255)
                                     gAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTaa                                                                                                          >  2:317634/1‑149 (MQ=255)
                                     gAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAAt                                                                                                         >  2:845045/1‑150 (MQ=255)
                                      aaTACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAAt                                                                                                         >  1:187206/1‑149 (MQ=255)
                                        tACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCTCGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAg                                                                                                       <  1:1124454/149‑1 (MQ=255)
                                           ttGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCt                                                                                                  >  2:680070/1‑151 (MQ=255)
                                            tGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAAcc                                                                                                   <  2:405962/149‑1 (MQ=255)
                                             gcgcAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTg                                                                                                 <  2:1198437/150‑1 (MQ=255)
                                             gcgcAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTg                                                                                                 <  1:484891/150‑1 (MQ=255)
                                             gcgcAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGc                                                                                                >  2:714662/1‑151 (MQ=255)
                                              cgcAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCg                                                                                               <  2:395581/151‑1 (MQ=255)
                                                  aaaCCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGatat                                                                                           <  2:900526/151‑1 (MQ=255)
                                                       aGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCt                                                                                      <  1:12273/151‑1 (MQ=255)
                                                           ttCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTa                                                                                   <  1:257822/150‑1 (MQ=255)
                                                           ttCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTAc                                                                                  >  2:598631/1‑151 (MQ=255)
                                                            tCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTAc                                                                                  <  2:929166/150‑1 (MQ=255)
                                                             cGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACg                                                                                 <  1:94022/150‑1 (MQ=255)
                                                                      gTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGtcatca                                                                       <  2:1228246/151‑1 (MQ=255)
                                                                      gTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGtcatc                                                                        >  2:1092350/1‑150 (MQ=255)
                                                                         tGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACg                                                                     <  2:421260/150‑1 (MQ=255)
                                                                          gCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCt                                                                   >  2:848380/1‑151 (MQ=255)
                                                                           cAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTg                                                                  <  2:367683/151‑1 (MQ=255)
                                                                            aGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTGCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGa                                                                 <  1:482609/151‑1 (MQ=255)
                                                                             ggCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGAc                                                                <  1:180061/151‑1 (MQ=255)
                                                                               cTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGt                                                              >  2:1167101/1‑151 (MQ=255)
                                                                                tCCTGGCACTGCATTCCAGAAACGTCAACCAAGATAACGGGTTTGTCTTTGTACTTCTCAAGTCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAGTAGAATCTGCGATATGTCTATTATGGAAGTCATCACGCTGAc                                                                >  1:187704/1‑148 (MQ=255)
                                                                                 ccTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGt                                                              <  1:1264768/149‑1 (MQ=255)
                                                                                 ccTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGt                                                              <  1:313506/149‑1 (MQ=255)
                                                                                 ccTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTa                                                             <  1:84002/150‑1 (MQ=255)
                                                                                   tGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAAt                                                          >  2:1275561/1‑151 (MQ=255)
                                                                                    ggCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTaaa                                                           <  2:282506/149‑1 (MQ=255)
                                                                                          gCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCcaca                                                     <  1:529075/149‑1 (MQ=255)
                                                                                          gCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACaa                                                    <  2:1235275/150‑1 (MQ=255)
                                                                                             tGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACcac                                                >  1:368050/1‑151 (MQ=255)
                                                                                                     ccGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGtctt                                        >  2:146469/1‑151 (MQ=255)
                                                                                                            ccACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTttgtt                                 >  1:603768/1‑151 (MQ=255)
                                                                                                                 aTAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATc                             >  1:550291/1‑150 (MQ=255)
                                                                                                                 aTAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCa                            <  1:751407/151‑1 (MQ=255)
                                                                                                                  tAACCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATc                             >  1:72350/1‑149 (MQ=255)
                                                                                                                    aCCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGAc                         <  1:592335/151‑1 (MQ=255)
                                                                                                                    aCCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGAc                         >  1:398519/1‑151 (MQ=255)
                                                                                                                    aCCGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGAc                         >  2:1266595/1‑151 (MQ=255)
                                                                                                                     ccGGTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGAcg                        >  2:323082/1‑151 (MQ=255)
                                                                                                                       ggTTTGTCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGAcgcg                      <  2:410954/151‑1 (MQ=255)
                                                                                                                             tCTTTGTGCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCtt                >  2:1281083/1‑151 (MQ=255)
                                                                                                                                 tgtgCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTcac             <  2:505172/150‑1 (MQ=255)
                                                                                                                                    gCTTCTCAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACg         <  2:1250633/151‑1 (MQ=255)
                                                                                                                                       tctcAAGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAg       <  2:880000/150‑1 (MQ=255)
                                                                                                                                          cAAGCCCACCAGCATTATGGGCTTGAATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGTCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTAATCAGACGCGTAGCTTCTCCTCGATTa     <  1:313688/149‑1 (MQ=255)
                                                                                                                                           aaGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATc  >  2:1220393/1‑151 (MQ=255)
                                                                                                                                           aaGCCCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATc  <  2:1003394/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTCTTTGTGCTTCTCAAGCTCACCAACATTGTTGGCTTTGATTTCGCTCGGCAACAGATTAATAGAACCTGCGATATGGCCTTTACGGAAGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATC  >  NC_000913/3784610‑3784899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: