Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	488,628	T→C	V698A (GTG→GCG)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	488,628	0	T	C	100.0%	314.9 / NA	86	V698A (GTG→GCG)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/39);  total (47/39)

TTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTT  >  NC_000913/488481‑488772                                                                                                                                                    |                                                                                                                                                 tttCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCt                                                                                                                                               >  1:163503/1‑151 (MQ=255)  ttCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTg                                                                                                                                              >  1:23140/1‑151 (MQ=255)   tCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTg                                                                                                                                              <  1:253421/150‑1 (MQ=255)         cTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATa                                                                                                                                        >  2:79234/1‑150 (MQ=255)           gCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATc                                                                                                                                     >  1:108551/1‑151 (MQ=255)             aTCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATccc                                                                                                                                   >  2:1054212/1‑151 (MQ=255)              tCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATc                                                                                                                                     >  1:613576/1‑148 (MQ=255)               cTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCg                                                                                                                                  >  2:1134105/1‑150 (MQ=255)                tGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGAt                                                                                                                                >  2:64907/1‑151 (MQ=255)                 gatggatgATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATccc                                                                                                                                   <  2:98959/147‑1 (MQ=255)                 gatggatgATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGAtt                                                                                                                               <  2:320405/151‑1 (MQ=255)                 gatggatgATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGAt                                                                                                                                <  1:516733/150‑1 (MQ=255)                 gatggatgATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCCATAATCCCGAt                                                                                                                                <  1:611847/150‑1 (MQ=255)                    ggatgATGTGCTGGGGCACGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGTTGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCCCGCTGTCGATAATccc                                                                                                                                   >  1:994681/1‑144 (MQ=255)                    ggaggaGGTGCAGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCATCCTGGTATGGCCGATGTACCGCGAAAGCTGGCGTGAGAAGGAGTCGCACACCATGCGACTAGTCACCATGACCGCGCTGTCGATAATCCCGATTg                                                                                                                              <  1:672441/149‑1 (MQ=255)                      atgatgTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCt                                                                                                                          <  2:1133802/151‑1 (MQ=255)                      atgatgTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCt                                                                                                                          >  2:938786/1‑151 (MQ=255)                        gatgTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTg                                                                                                                         <  2:512432/150‑1 (MQ=255)                          tgtgCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGAt                                                                                                                       <  1:970415/150‑1 (MQ=255)                              cTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGc                                                                                                                  >  2:3509/1‑151 (MQ=255)                                     aaGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGc                                                                                                           >  2:486190/1‑151 (MQ=255)                                         gatgatTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACa                                                                                                       <  2:345721/151‑1 (MQ=255)                                             atTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCt                                                                                                   <  1:448582/151‑1 (MQ=255)                                             atTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGAATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCt                                                                                                   >  1:1104174/1‑151 (MQ=255)                                              ttttCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGAGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCt                                                                                                   >  1:606629/1‑150 (MQ=255)                                               tttCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTAc                                                                                                 >  2:636917/1‑151 (MQ=255)                                                ttcttcAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACt                                                                                                >  2:1098291/1‑151 (MQ=255)                                                     cAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTac                                                                                           >  1:976678/1‑151 (MQ=255)                                                     cAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTac                                                                                           >  1:1190667/1‑151 (MQ=255)                                                        ccTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACt                                                                                        >  2:1069778/1‑151 (MQ=255)                                                          tgctgctgATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTAc                                                                                      >  1:277355/1‑151 (MQ=255)                                                          tgctgctgATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTAc                                                                                      >  1:849383/1‑151 (MQ=255)                                                           gctgctgATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACg                                                                                     <  2:963596/151‑1 (MQ=255)                                                              gctgATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTCCACTACGCTg                                                                                  <  1:647618/151‑1 (MQ=255)                                                               ctgATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGc                                                                                 >  2:471349/1‑151 (MQ=255)                                                                 gATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCg                                                                                <  1:190610/150‑1 (MQ=255)                                                                  aTTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTc                                                                              <  2:1008954/151‑1 (MQ=255)                                                                    tGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTg                                                                            >  1:953432/1‑151 (MQ=255)                                                                     gCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTGCTTCTACACTCCGCTGCGTCTgg                                                                           <  1:529660/151‑1 (MQ=255)                                                                     gCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTgg                                                                           <  1:1142222/151‑1 (MQ=255)                                                                     gCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTg                                                                            <  1:958526/150‑1 (MQ=255)                                                                         tCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAgg                                                                       >  1:613885/1‑151 (MQ=255)                                                                         tCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAg                                                                        <  1:1230910/150‑1 (MQ=255)                                                                          ccTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAgg                                                                       <  1:1036898/150‑1 (MQ=255)                                                                            tGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACg                                                                    >  2:1119331/1‑151 (MQ=255)                                                                             ggTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGAc                                                                     <  2:531868/149‑1 (MQ=255)                                                                                aTGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTgg                                                                >  1:6793/1‑151 (MQ=255)                                                                                  ggCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGAt                                                              >  1:147926/1‑151 (MQ=255)                                                                                  ggCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGAt                                                              >  1:306705/1‑151 (MQ=255)                                                                                   gCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGAtt                                                             >  2:7431/1‑151 (MQ=255)                                                                                    ccGATTTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTg                                                            >  1:391401/1‑151 (MQ=255)                                                                                     cGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTg                                                            >  2:279975/1‑150 (MQ=255)                                                                                     cGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTg                                                            >  1:411995/1‑150 (MQ=255)                                                                                     cGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTg                                                            >  2:300037/1‑150 (MQ=255)                                                                                      gATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGaa                                                          <  1:695917/151‑1 (MQ=255)                                                                                             cgcgAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGttt                                                   >  1:314261/1‑151 (MQ=255)                                                                                              gcgAAAGCTGGCGTTATAAAGAGTCGCACACCATGCGACTGGTCACTATTACCGCGCTGTCGATAATCCCCATTTCGCTAATGGTGCTGACTGCTACAGGCTACTTCTACAATACGCTGCGTCTTGCAGGACGCTGGATTGACACCGTTTa                                                  <  1:76061/151‑1 (MQ=255)                                                                                                gAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATc                                                <  1:1210638/151‑1 (MQ=255)                                                                                                 aaaGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCt                                               <  1:903211/151‑1 (MQ=255)                                                                                                 aaaGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCt                                               >  1:599060/1‑151 (MQ=255)                                                                                                  aaGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTg                                              >  1:1269361/1‑151 (MQ=255)                                                                                                  aaGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTg                                              <  2:814932/151‑1 (MQ=255)                                                                                                    gCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTgg                                             >  1:160077/1‑150 (MQ=255)                                                                                                    gCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGt                                            <  2:237217/151‑1 (MQ=255)                                                                                                       ggCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGa                                          <  2:808172/150‑1 (MQ=255)                                                                                                         cGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGatca                                       <  2:544397/151‑1 (MQ=255)                                                                                                         cGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGatc                                        >  2:822668/1‑150 (MQ=255)                                                                                                          gTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGatcat                                      <  1:500149/151‑1 (MQ=255)                                                                                                            gATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCt                                    >  2:703091/1‑151 (MQ=255)                                                                                                               aaaGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTgg                                  >  2:245878/1‑150 (MQ=255)                                                                                                                aaGAGTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGa                                 >  1:937224/1‑150 (MQ=255)                                                                                                                 agagTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAAc                               >  1:531695/1‑151 (MQ=255)                                                                                                                  gagTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAAcc                              <  1:393406/151‑1 (MQ=255)                                                                                                                  gagTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAAcc                              >  1:343382/1‑151 (MQ=255)                                                                                                                   agTCGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACct                             >  1:1192956/1‑151 (MQ=255)                                                                                                                      cGCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACctgc                           <  2:690864/150‑1 (MQ=255)                                                                                                                       gCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACctgctg                         <  1:503804/151‑1 (MQ=255)                                                                                                                       gCACACCATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACctgctg                         <  2:984409/151‑1 (MQ=255)                                                                                                                            ccATGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCa                    <  1:1054170/151‑1 (MQ=255)                                                                                                                              aTGCGACTGGTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTAcc                     >  2:380068/1‑148 (MQ=255)                                                                                                                                       gTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGc         <  1:367130/151‑1 (MQ=255)                                                                                                                                       gTCACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGc         >  1:519726/1‑151 (MQ=255)                                                                                                                                         cACCATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCg        <  2:407618/150‑1 (MQ=255)                                                                                                                                           ccATTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGACCTTGCTGTCCCAGACCGTTCTGCGTg      <  1:919913/150‑1 (MQ=255)                                                                                                                                             aTTACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCt   >  2:649048/1‑151 (MQ=255)                                                                                                                                               tACCGCGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGTAACCTGCTGTACCAGACGGTACTGCGTGGCtt  <  1:571980/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTT  >  NC_000913/488481‑488772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑