Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,624	A→G	A49A (GCA→GCG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,624	0	A	G	100.0%	259.2 / NA	71	A49A (GCA→GCG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/48);  total (23/48)

CGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTA  >  NC_000913/4215476‑4215771                                                                                                                                                     |                                                                                                                                                    cGATGACTGATCAGGCAACAACAACCGATGAACTCGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTGCTggcggc                                                                                                                                                   <  1:1051697/151‑1 (MQ=255)       cTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGTAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCAt                                                                                                                                             >  1:907798/1‑151 (MQ=255)         gAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCAt                                                                                                                                             <  2:1284365/149‑1 (MQ=255)         gAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCAt                                                                                                                                             <  2:477487/149‑1 (MQ=255)          aaCAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTca                                                                                                                                          <  2:167687/151‑1 (MQ=255)            cAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTcagc                                                                                                                                        <  1:786715/151‑1 (MQ=255)                    aacaacCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCaa                                                                                                                                <  1:720518/151‑1 (MQ=255)                     acaacCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGTAGCAGCAAg                                                                                                                               <  2:1206306/151‑1 (MQ=255)                      caacCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGa                                                                                                                              <  2:823191/151‑1 (MQ=255)                        acCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGata                                                                                                                            <  1:724194/151‑1 (MQ=255)                            aTGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTg                                                                                                                         >  1:505278/1‑150 (MQ=255)                               agcTGGCTTTCACAAGGCCGTATGGCGAGCAGGATAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCATCAGCAGTATGATATTGAt                                                                                                                       <  1:504196/147‑1 (MQ=255)                               aaCTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATa                                                                                                                      <  2:736945/150‑1 (MQ=255)                                aCTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACACCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAAc                                                                                                                    <  1:1017123/151‑1 (MQ=255)                                      tttCACAAGGCCGTATGGCGAGCAGGAGAAGCACATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGGGCGCATTCAGCAGCAGCAAGATATTGATAACGGAAc                                                                                                               >  1:884905/1‑150 (MQ=255)                                       ttCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGATCg                                                                                                              <  2:891752/150‑1 (MQ=255)                                       ttCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACg                                                                                                              <  2:242959/150‑1 (MQ=255)                                        tCACAAGGCCGTATTGCGAGCAGGAGAAGCAAATTCTTACTGCCGCAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGtt                                                                                                            >  1:954153/1‑151 (MQ=255)                                         cacaAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTg                                                                                                           <  1:71743/151‑1 (MQ=255)                                         cacaAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTg                                                                                                           <  1:882007/151‑1 (MQ=255)                                           caAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGcc                                                                                                         >  2:456817/1‑151 (MQ=255)                                            aaGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCt                                                                                                        >  2:289221/1‑151 (MQ=255)                                                 cGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGAttt                                                                                                   <  2:577428/151‑1 (MQ=255)                                                  gTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGAtttt                                                                                                  >  1:468725/1‑151 (MQ=255)                                                      ggCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTAttt                                                                                              <  2:217905/151‑1 (MQ=255)                                                        cGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCg                                                                                            <  2:171722/151‑1 (MQ=255)                                                        cGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCg                                                                                            <  2:1013890/151‑1 (MQ=255)                                                         gAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCgg                                                                                           <  1:878489/151‑1 (MQ=255)                                                          aGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGa                                                                                          <  2:1085202/151‑1 (MQ=255)                                                            cAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGaaa                                                                                        <  1:652737/151‑1 (MQ=255)                                                            cAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGaaa                                                                                        <  2:393650/151‑1 (MQ=255)                                                                 gaAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCt                                                                                   <  2:933959/151‑1 (MQ=255)                                                                   aGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTc                                                                                 <  1:1254195/151‑1 (MQ=255)                                                                     cAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCa                                                                               <  1:410112/151‑1 (MQ=255)                                                                         ttCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTcg                                                                           >  1:74745/1‑151 (MQ=255)                                                                             tACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTcgcg                                                                         <  2:107515/149‑1 (MQ=255)                                                                             tACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTcgcg                                                                         <  2:1152423/149‑1 (MQ=255)                                                                             tACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGAt                                                                       >  1:797518/1‑151 (MQ=255)                                                                                  ccGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGa                                                                  <  2:147551/151‑1 (MQ=255)                                                                                   cGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGAt                                                                 <  1:487553/151‑1 (MQ=255)                                                                                     aaGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTg                                                               >  2:690503/1‑151 (MQ=255)                                                                                     aaGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTg                                                               <  2:578876/151‑1 (MQ=255)                                                                                         ggTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGaaa                                                           <  1:791405/151‑1 (MQ=255)                                                                                          gTAGAATTTCTGTCTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAGGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGaa                                                            <  2:191685/149‑1 (MQ=255)                                                                                          gTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGaaaa                                                          <  2:66815/151‑1 (MQ=255)                                                                                            aGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAAtt                                                        <  1:245230/151‑1 (MQ=255)                                                                                              aaTTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTcg                                                      <  2:501480/151‑1 (MQ=255)                                                                                               aTTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTcgc                                                     >  2:1211047/1‑151 (MQ=255)                                                                                                    tgactgaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGAt                                                >  2:599620/1‑151 (MQ=255)                                                                                                    tgactgaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGAt                                                >  1:1035536/1‑151 (MQ=255)                                                                                                         gaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTcc                                             <  1:123206/149‑1 (MQ=255)                                                                                                         gaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGAGTcca                                            <  1:83416/150‑2 (MQ=255)                                                                                                          aGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGc                                          >  1:922099/1‑151 (MQ=255)                                                                                                           gCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCg                                         <  2:29957/151‑1 (MQ=255)                                                                                                            cTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCgg                                        >  1:407400/1‑151 (MQ=255)                                                                                                                 gACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCTGACtt                                    <  1:1177775/150‑1 (MQ=255)                                                                                                                 gACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACtt                                    <  2:820155/150‑1 (MQ=255)                                                                                                                 gACGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTa                                   <  1:1059852/151‑1 (MQ=255)                                                                                                                   cGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACtt                                    >  1:128188/1‑148 (MQ=255)                                                                                                                   cGCATTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTGCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTaga                                 <  1:923540/151‑1 (MQ=255)                                                                                                                      aTTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTaga                                 >  1:732165/1‑148 (MQ=255)                                                                                                                      aTTTTACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTaga                                 >  2:1220128/1‑148 (MQ=255)                                                                                                                         ttACGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGAccg                           >  2:534134/1‑151 (MQ=255)                                                                                                                            cGCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCGTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGAccgt                          <  1:944054/149‑2 (MQ=255)                                                                                                                             gCCACAACGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACcgccgc                       <  2:29919/151‑1 (MQ=255)                                                                                                                                    cGCAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAgaga                <  2:487087/151‑1 (MQ=255)                                                                                                                                      cAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATa              <  2:583616/151‑1 (MQ=255)                                                                                                                                      cAATAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATa              <  2:498230/151‑1 (MQ=255)                                                                                                                                       aaTAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATa              >  1:537360/1‑150 (MQ=255)                                                                                                                                        aTAAACTTCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAAc            >  1:1076955/1‑151 (MQ=255)                                                                                                                                              ttCTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGccc      >  2:867961/1‑151 (MQ=255)                                                                                                                                                cTGGCGGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCgg    >  2:647476/1‑151 (MQ=255)                                                                                                                                                  ggcggcGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTa  <  1:977926/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTA  >  NC_000913/4215476‑4215771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑