Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,254,557	A→G	V637A (GTA→GCA)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,254,557	0	A	G	100.0%	300.3 / NA	81	V637A (GTA→GCA)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/43);  total (38/43)

AACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATACGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTT  >  NC_000913/4254410‑4254702                                                                                                                                                    |                                                                                                                                                  aaCTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGt                                                                                                                                                <  2:1029830/151‑1 (MQ=255)   cTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTcg                                                                                                                                              >  1:473791/1‑151 (MQ=255)    tcatcaTCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTcgc                                                                                                                                             >  1:422473/1‑151 (MQ=255)     catcatCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTcgcg                                                                                                                                            <  1:416330/151‑1 (MQ=255)      atcatcTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCCGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTcgcg                                                                                                                                            <  1:206604/150‑1 (MQ=255)      atcatcTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCgg                                                                                                                                           >  1:717798/1‑151 (MQ=255)       tcatcTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCggg                                                                                                                                          >  1:933682/1‑151 (MQ=255)        catcTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGga                                                                                                                                         <  1:1119558/151‑1 (MQ=255)         atcTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCggg                                                                                                                                          >  2:119029/1‑149 (MQ=255)             tGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGGAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGgaga                                                                                                                                       <  2:154538/148‑1 (MQ=255)              gCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGAtgtg                                                                                                                                   >  2:259634/1‑151 (MQ=255)                aGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGc                                                                                                                                 <  2:729806/151‑1 (MQ=255)                     aaTCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGc                                                                                                                            <  2:667726/151‑1 (MQ=255)                       tCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATACCGTCCGGCAACTCGTCGCGATCCCAGCGCAGGCACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTg                                                                                                                          >  1:82891/1‑151 (MQ=255)                         aGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTggg                                                                                                                        <  2:1118396/151‑1 (MQ=255)                              atGACGTTGCATCTCGTTAACGAGCGCATCAATAACGTCCGGCAACTCCTCGCGATCCCAGCGCAGGAACAGCTACGCTTTCAGTATTGGGTCAAGCACATTGACGTGCTCCCTCAATGCGTCGCGGGATATGTGGCGATGCTGGGTGACg                                                                                                                   >  1:88614/2‑151 (MQ=255)                               tGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACg                                                                                                                   >  2:1263469/1‑150 (MQ=255)                               tGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACg                                                                                                                   >  1:1055652/1‑150 (MQ=255)                                        aTCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTgccgcc                                                                                                          >  1:1065134/1‑150 (MQ=255)                                            cATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCa                                                                                                         <  2:1130243/147‑1 (MQ=255)                                            cATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAg                                                                                                     <  1:17884/151‑1 (MQ=255)                                               ttGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCg                                                                                                   <  2:1123936/150‑1 (MQ=255)                                               ttGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCg                                                                                                   <  2:32822/150‑1 (MQ=255)                                                 gCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAg                                                                                                <  2:800216/151‑1 (MQ=255)                                                   cAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGgc                                                                                              <  1:426471/151‑1 (MQ=255)                                                   cAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGgc                                                                                              <  2:595598/151‑1 (MQ=255)                                                         aTCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCAcc                                                                                        >  1:1182928/1‑151 (MQ=255)                                                          tCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCAcc                                                                                        >  1:1221452/1‑150 (MQ=255)                                                              tcaCGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAATCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACa                                                                                    <  1:779699/148‑1 (MQ=255)                                                                 cGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACatat                                                                                 <  2:417970/150‑1 (MQ=255)                                                                  gTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATgc                                                                               >  2:91646/1‑151 (MQ=255)                                                                    ccGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATgcgc                                                                             >  2:810044/1‑151 (MQ=255)                                                                      ggcagtttgtcgcggtcgcgGGGGAGGAAGAGGTGCGTTTTCAGCGTTGGGGAAAGCGCATTGACGTGGTCCATCAATGCGTGGCGGGGGATGTGGCGATGCTGGGTGACGATTGCCGCCATGAGCGTAGGTAGCGCCAACATATGCGCa                                                                            <  1:380792/142‑1 (MQ=255)                                                                         aaCTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAAtgt                                                                        >  1:405232/1‑151 (MQ=255)                                                                         aaCTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAAtgt                                                                        <  2:506464/151‑1 (MQ=255)                                                                          aCTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAAtgtt                                                                       >  2:916319/1‑151 (MQ=255)                                                                           cTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATg                                                                                <  2:967179/141‑1 (MQ=255)                                                                           cTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATg                                                                                >  1:967143/1‑141 (MQ=255)                                                                                cgcgATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCg                                                                 <  2:1182962/151‑1 (MQ=255)                                                                                  cgATCCCAGCGCAGGAACATCTCCGCTTTCAGAATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGTTGTGGCGATGCTGGGGGACGATTGCCGCTATCAGAGTAGGCAGCACCAACATATGCGCGATGTTCTTGCGAt                                                               >  1:1008942/1‑151 (MQ=255)                                                                                  cgATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGAt                                                               <  2:674780/151‑1 (MQ=255)                                                                                     tCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGAtagt                                                            >  2:1140907/1‑151 (MQ=255)                                                                                      cccAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGAtagta                                                           <  2:570266/151‑1 (MQ=255)                                                                                         aGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCTATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGt                                                        >  1:679249/1‑151 (MQ=255)                                                                                          gcgcAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGtc                                                       >  2:2046/1‑151 (MQ=255)                                                                                           cgcAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGt                                                        <  2:745058/149‑1 (MQ=255)                                                                                              aGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGtcatc                                                    >  1:739990/1‑150 (MQ=255)                                                                                               ggAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAg                                                  <  1:119036/151‑1 (MQ=255)                                                                                                gAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGc                                                 <  2:299922/151‑1 (MQ=255)                                                                                                 aaCAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGc                                                 >  1:87422/1‑150 (MQ=255)                                                                                                 aaCAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCa                                                <  2:384129/151‑1 (MQ=255)                                                                                                   cAGATCCGCTTTCCGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGCCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGCAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCa                                                <  1:268990/149‑1 (MQ=255)                                                                                                     gCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGc                                            >  1:18668/1‑151 (MQ=255)                                                                                                          gCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCt                                       <  2:245868/151‑1 (MQ=255)                                                                                                             ttCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTcgc                                    >  1:957086/1‑151 (MQ=255)                                                                                                             ttCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTc                                      <  1:578097/149‑1 (MQ=255)                                                                                                             ttCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTc                                      <  2:346039/149‑1 (MQ=255)                                                                                                                  cATTGGGTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGc                               <  1:545092/151‑1 (MQ=255)                                                                                                                       ggTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAat                          <  1:387275/151‑1 (MQ=255)                                                                                                                        gTAAGGCACATTGACGTGCTCCATCAATGCGTCGCGGGATATGTGGCGATGCTGGGTGACGATTGCCGCCATAAACGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatg                         >  1:538339/1‑151 (MQ=255)                                                                                                                        gTAAAGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatg                         <  2:191478/151‑1 (MQ=255)                                                                                                                          aaaGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgat                       <  1:29784/151‑1 (MQ=255)                                                                                                                          aaaGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgat                       <  2:107576/151‑1 (MQ=255)                                                                                                                          aaaGCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatga                        >  1:492488/1‑150 (MQ=255)                                                                                                                          aaaGAACATTGACGGGCTCCATCAATGCGGCGCGGGAGATGTGGCGATGGTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgat                       >  1:205540/1‑151 (MQ=255)                                                                                                                             gCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgatgat                    <  2:1036065/151‑1 (MQ=255)                                                                                                                             gCACATTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgatgat                    <  2:717830/151‑1 (MQ=255)                                                                                                                               acaTTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAatgatgatg                   >  1:723603/1‑150 (MQ=255)                                                                                                                                caTTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGGCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTc                 <  1:628900/151‑1 (MQ=255)                                                                                                                                 aTTGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCg                >  2:605321/1‑151 (MQ=255)                                                                                                                                  ttGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCg                >  2:723531/1‑150 (MQ=255)                                                                                                                                   tGACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGcc              >  2:907349/1‑151 (MQ=255)                                                                                                                                    gACGTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCg             <  1:1121413/151‑1 (MQ=255)                                                                                                                                       gTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGAtt          <  1:625482/151‑1 (MQ=255)                                                                                                                                       gTGCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGAtt          >  2:1138047/1‑151 (MQ=255)                                                                                                                                         gCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtgt        >  2:535708/1‑151 (MQ=255)                                                                                                                                         gCTCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtg         <  2:520049/150‑1 (MQ=255)                                                                                                                                           tCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTgtgt      >  1:922610/1‑151 (MQ=255)                                                                                                                                           tCCATCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTACCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTgtgt      >  2:90775/1‑151 (MQ=255)                                                                                                                                              aTCAATGCGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCtt   >  2:277102/1‑151 (MQ=255)                                                                                                                                                cAATGCGTAGCGGGAGACGTGGCGATGCTGGGTGACGATTGCGGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGGTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCttt  <  1:166402/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAGCGCATCAATAACGTCCGGCAACTCGTCGCGATCCCAGCGCAGGAACAGCTCCGCTTTCAGCATTGGGTAAAGCACATTGACGTGCTCCATCAATACGTCGCGGGAGATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTT  >  NC_000913/4254410‑4254702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑