Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,187	A→G	K485K (AAA→AAG)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,187	0	A	G	100.0%	317.4 / NA	84	K485K (AAA→AAG)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/37);  total (47/37)

AGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGA  >  NC_000913/4632039‑4632332                                                                                                                                                     |                                                                                                                                                  agagCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCg                                                                                                                                                 >  2:101651/1‑151 (MQ=255) agagCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCg                                                                                                                                                 <  2:508208/151‑1 (MQ=255)       aGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTacac                                                                                                                                           >  1:382224/1‑151 (MQ=255)          cAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCTCTACACCa                                                                                                                                         <  1:690863/150‑1 (MQ=255)          cAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACAcc                                                                                                                                          >  1:151727/1‑149 (MQ=255)          cAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACAcc                                                                                                                                          >  2:666539/1‑149 (MQ=255)          cAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAg                                                                                                                                        <  2:270766/151‑1 (MQ=255)           aaaaaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGc                                                                                                                                       >  1:947205/1‑151 (MQ=255)            aaaaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCg                                                                                                                                      >  2:493999/1‑151 (MQ=255)             aaaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCgg                                                                                                                                     >  1:956589/1‑151 (MQ=255)              aaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCgg                                                                                                                                     >  1:121081/1‑150 (MQ=255)               aCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTa                                                                                                                                   <  1:332649/151‑1 (MQ=255)                cAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTa                                                                                                                                   >  1:900477/1‑150 (MQ=255)                 agagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTaa                                                                                                                                  <  2:1084600/150‑1 (MQ=255)                 agagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTa                                                                                                                                   >  1:327303/1‑149 (MQ=255)                 agagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAg                                                                                                                                 <  2:526740/151‑1 (MQ=255)                 agagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAg                                                                                                                                 <  1:748368/151‑1 (MQ=255)                  gagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAgg                                                                                                                                >  1:245811/1‑151 (MQ=255)                    gCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGga                                                                                                                               <  1:126742/150‑1 (MQ=255)                      aGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAgg                                                                                                                                >  2:181218/1‑147 (MQ=255)                       ggCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAgg                                                                                                                                >  2:580229/1‑146 (MQ=255)                        gCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATcc                                                                                                                          <  2:1051590/151‑1 (MQ=255)                         cTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATccc                                                                                                                         >  2:1044249/1‑151 (MQ=255)                          tCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCg                                                                                                                        >  1:803555/1‑151 (MQ=255)                           cAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGc                                                                                                                       >  2:855567/1‑151 (MQ=255)                             aCTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCaa                                                                                                                     <  2:41803/151‑1 (MQ=255)                             aCTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCa                                                                                                                      >  2:600769/1‑150 (MQ=255)                               tGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCaa                                                                                                                     >  2:618925/1‑149 (MQ=255)                                ggCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAg                                                                                                                   <  2:655817/150‑1 (MQ=255)                                   gcgGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTAAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTAt                                                                                                               >  1:1193765/1‑151 (MQ=255)                                    cgGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATg                                                                                                              <  1:349515/151‑1 (MQ=255)                                    cgGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATg                                                                                                              <  1:1089870/151‑1 (MQ=255)                                                aacaacCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTg                                                                                                  >  1:96279/1‑151 (MQ=255)                                                 acaacCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGc                                                                                                 <  2:603588/151‑1 (MQ=255)                                                   aacCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGTTc                                                                                               >  1:898285/1‑151 (MQ=255)                                                    acCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCg                                                                                              >  2:290197/1‑151 (MQ=255)                                                       aaTGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGt                                                                                             >  1:1268845/1‑149 (MQ=255)                                                              ggATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAgc                                                                                    <  1:1242367/151‑1 (MQ=255)                                                                  cTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGcc                                                                                 >  1:12390/1‑150 (MQ=255)                                                                     aGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTgg                                                                              <  1:101762/150‑1 (MQ=255)                                                                     aGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGa                                                                             <  2:434811/151‑1 (MQ=255)                                                                         ttCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATcc                                                                         >  1:575655/1‑151 (MQ=255)                                                                           cAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCg                                                                        <  1:503821/150‑1 (MQ=255)                                                                            aGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGa                                                                       >  1:1223225/1‑150 (MQ=255)                                                                                 aCGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTg                                                                  >  1:35354/1‑150 (MQ=255)                                                                                 aCGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGa                                                                 <  1:988446/151‑1 (MQ=255)                                                                                   gATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGTAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGaa                                                                >  2:576086/1‑150 (MQ=255)                                                                                   gATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGaa                                                                >  1:1107648/1‑150 (MQ=255)                                                                                       gCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCAc                                                           >  2:515405/1‑151 (MQ=255)                                                                                            gAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTa                                                      <  2:475144/151‑1 (MQ=255)                                                                                            gAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTa                                                      <  2:1082740/151‑1 (MQ=255)                                                                                              aGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAgg                                                    >  1:299759/1‑151 (MQ=255)                                                                                               gCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAggg                                                   >  1:1234588/1‑151 (MQ=255)                                                                                                  gtgGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGcc                                                <  2:1253877/151‑1 (MQ=255)                                                                                                    gggATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGcc                                                >  2:1250904/1‑149 (MQ=255)                                                                                                     ggATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGc                                             <  1:1201515/151‑1 (MQ=255)                                                                                                      gATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCg                                            <  2:407944/151‑1 (MQ=255)                                                                                                       atcatcTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCgg                                           <  2:549030/151‑1 (MQ=255)                                                                                                          atcTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCtt                                        >  2:1148232/1‑151 (MQ=255)                                                                                                              ggAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCtt                                        <  2:900518/147‑1 (MQ=255)                                                                                                              ggAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATg                                    <  2:35349/151‑1 (MQ=255)                                                                                                                aaGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGc                                   >  2:1019147/1‑150 (MQ=255)                                                                                                                aaGAGCGATTCCCGCCGGCTTACAACGCTCTTCTCAAGCGCTACACCAGCGGTACGTAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGc                                   >  1:1118342/1‑150 (MQ=255)                                                                                                                 agagCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGc                                   >  2:451377/1‑149 (MQ=255)                                                                                                                   agCGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGc                                   >  2:659848/1‑147 (MQ=255)                                                                                                                     cGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGAtt                              <  2:1035677/150‑1 (MQ=255)                                                                                                                     cGATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCCCAAAGCTATGCGATGGCGATTGCTCGTCAGGACAGCGCCTGGCATCCGAACGTGAACTCACCGGAAGGGCCCAGCAGCTTGATGCa                                  >  1:1201683/1‑146 (MQ=255)                                                                                                                      gATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTa                             <  1:1143886/150‑1 (MQ=255)                                                                                                                      gATTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTAt                            >  2:578061/1‑151 (MQ=255)                                                                                                                       aTTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATg                           >  2:418038/1‑151 (MQ=255)                                                                                                                       aTTCCCGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATg                           >  1:923479/1‑151 (MQ=255)                                                                                                                          cccGCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCt                        <  1:1041344/151‑1 (MQ=255)                                                                                                                             gCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGTTTGATGCAGATTATGCCTGGt                     <  1:254268/151‑1 (MQ=255)                                                                                                                             gCTGGCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGt                     >  2:774390/1‑151 (MQ=255)                                                                                                                                ggCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTAc                   >  1:472991/1‑150 (MQ=255)                                                                                                                                 gCTTACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAg                 <  2:327326/151‑1 (MQ=255)                                                                                                                                   ttACAACGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCg               >  1:1117050/1‑151 (MQ=255)                                                                                                                                       aaCGATCTTTTCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGAccc           <  2:151727/151‑1 (MQ=255)                                                                                                                                              ttttCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACgg     >  1:809481/1‑150 (MQ=255)                                                                                                                                               tttCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATa        <  1:11755/146‑1 (MQ=255)                                                                                                                                               tttCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACgg     <  2:447784/149‑1 (MQ=255)                                                                                                                                                ttCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTg   >  2:1166760/1‑150 (MQ=255)                                                                                                                                                 tCAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGa  <  1:855530/150‑1 (MQ=255)                                                                                                                                                  cAAGCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGGCAGGAGAGCGCCTGGAATCCGAAATTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCAGACGGTGa  <  1:494472/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGA  >  NC_000913/4632039‑4632332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑