Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	741,429	A→G	S376S (TCT→TCC)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,429	0	A	G	100.0%	253.7 / NA	66	S376S (TCT→TCC)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCA  >  NC_000913/741282‑741574                                                                                                                                                    |                                                                                                                                                  gATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAggga                                                                                                                                                 >  2:1072754/1‑150 (MQ=255)    cgcgCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGggaaga                                                                                                                                              <  1:1039477/150‑1 (MQ=255)     gcgcCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAagag                                                                                                                                             >  2:249368/1‑150 (MQ=255)          aGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAc                                                                                                                                         >  1:865378/1‑149 (MQ=255)           gAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCACCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCa                                                                                                                                       <  1:285020/150‑1 (MQ=255)             aaGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGCAGAGTGACCATa                                                                                                                                     >  1:168075/1‑150 (MQ=255)                  gcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGc                                                                                                                               >  2:582743/1‑151 (MQ=255)                   caTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCg                                                                                                                              >  2:210263/1‑151 (MQ=255)                   caTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCg                                                                                                                              <  2:786965/151‑1 (MQ=255)                     tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCgg                                                                                                                             >  2:839310/1‑150 (MQ=255)                      gTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGa                                                                                                                            <  2:494180/150‑1 (MQ=255)                      gTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACAGGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGa                                                                                                                            <  2:971296/150‑1 (MQ=255)                        aGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGAcc                                                                                                                          <  2:739888/150‑1 (MQ=255)                        aGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGAcc                                                                                                                          <  1:855040/150‑1 (MQ=255)                         gATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAg                                                                                                                        <  1:115400/151‑1 (MQ=255)                          aTGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGCGGCAGGGAAGAGTGACCATACATTGCGGACCAGc                                                                                                                       >  1:950858/1‑151 (MQ=255)                              cGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGgc                                                                                                                   >  2:974805/1‑151 (MQ=255)                                      aCGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAgg                                                                                                           >  1:328351/1‑151 (MQ=255)                                      aCGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAgg                                                                                                           <  2:1028410/151‑1 (MQ=255)                                      aCGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAgg                                                                                                           >  2:410683/1‑151 (MQ=255)                                            gTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGaa                                                                                                     <  2:950050/151‑1 (MQ=255)                                                 acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGc                                                                                                  >  1:343182/1‑149 (MQ=255)                                                  ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGc                                                                                                  >  2:162730/1‑148 (MQ=255)                                                   cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAg                                                                                                >  2:1223283/1‑149 (MQ=255)                                                   cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGaa                                                                                              <  1:1145880/151‑1 (MQ=255)                                                     gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATc                                                                                            <  2:792035/151‑1 (MQ=255)                                                       gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCg                                                                                          <  1:582712/151‑1 (MQ=255)                                                       gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCg                                                                                          >  2:153024/1‑151 (MQ=255)                                                         tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGgc                                                                                        >  2:766440/1‑151 (MQ=255)                                                             aaTGCGCGTAATTTGCGACATGGCACCCGGGTCGATAAACAGTTCCGCAACGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCt                                                                                     <  1:1089091/150‑1 (MQ=255)                                                                  gcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCtcatca                                                                                <  2:1048016/150‑1 (MQ=255)                                                                   cgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCagg                                                                              >  2:782718/1‑151 (MQ=255)                                                                        tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggcca                                                                           >  1:834317/1‑149 (MQ=255)                                                                         ttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggccaag                                                                         <  2:309922/150‑1 (MQ=255)                                                                          tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggccaagg                                                                        <  1:756661/150‑1 (MQ=255)                                                                          tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggccaagg                                                                        <  2:1060861/150‑1 (MQ=255)                                                                          tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggccaagg                                                                        <  2:92091/150‑1 (MQ=255)                                                                             gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAggccaaggccaa                                                                    <  2:1028608/151‑1 (MQ=255)                                                                                 tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGc                                                                >  2:641555/1‑151 (MQ=255)                                                                                     aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAAt                                                            <  2:833878/151‑1 (MQ=255)                                                                                        cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTc                                                         >  2:32381/1‑151 (MQ=255)                                                                                         gggTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTc                                                         <  1:970151/150‑1 (MQ=255)                                                                                                 aaaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGGGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAg                                                   >  2:665865/1‑148 (MQ=255)                                                                                                 aaaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCACCCAGATg                                                >  2:785365/1‑151 (MQ=255)                                                                                                  aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgc                                               >  2:418061/1‑151 (MQ=255)                                                                                                    cAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCTCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcgc                                             <  2:1157679/151‑1 (MQ=255)                                                                                                          tgCAAAGCCCATCACCGCAAGGCCTAATACCATCAGTGGCAGGGAAGAGAGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGt                                        >  1:182134/2‑150 (MQ=255)                                                                                                               aGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGAtt                                  <  1:410653/151‑1 (MQ=255)                                                                                                                gCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGAtt                                  >  2:827831/1‑150 (MQ=255)                                                                                                                      cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgcg                           <  2:528397/151‑1 (MQ=255)                                                                                                                      cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgc                            <  1:236302/150‑1 (MQ=255)                                                                                                                        ccGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGa                          <  2:821239/150‑1 (MQ=255)                                                                                                                         ccgcAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGaca                        <  1:289574/148‑1 (MQ=255)                                                                                                                         cGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGac                         >  2:663763/1‑150 (MQ=255)                                                                                                                         cGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGac                         >  1:36776/1‑150 (MQ=255)                                                                                                                           ccAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACAct                      <  1:556355/151‑1 (MQ=255)                                                                                                                            cAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActc                     >  2:498806/1‑151 (MQ=255)                                                                                                                            cAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActc                     >  2:638690/1‑151 (MQ=255)                                                                                                                                ccTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtt                 >  2:579294/1‑151 (MQ=255)                                                                                                                                ccTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtt                 <  1:644778/151‑1 (MQ=255)                                                                                                                                   aaTACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtttt               <  2:1101518/150‑1 (MQ=255)                                                                                                                                   aaTACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCttt                <  1:1265415/149‑1 (MQ=255)                                                                                                                                     tACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTacc            >  2:219947/1‑151 (MQ=255)                                                                                                                                      aCCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTacc            >  2:919487/1‑150 (MQ=255)                                                                                                                                           cAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTAccacccac      <  1:249360/151‑1 (MQ=255)                                                                                                                                           cAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTAccacccac      <  1:1131135/151‑1 (MQ=255)                                                                                                                                               ggCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCa  <  1:285752/151‑1 (MQ=255)                                                                                                                                               ggCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCa  <  1:1072714/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCCA  >  NC_000913/741282‑741574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑