Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 755,804 A→G D82G (GAC→GGC)  sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,8040AG98.6% 243.9 / ‑3.8 71D82G (GAC→GGC) sdhDsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (35/35);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCC  >  NC_000913/755663‑755951
                                                                                                                                             |                                                                                                                                                   
gTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTa                                                                                                                                             >  1:936865/1‑150 (MQ=255)
      tCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTGCGCCTCTGCGTTCACCAAAGTGTTCGCCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAAcc                                                                                                                                         <  2:1061957/148‑1 (MQ=255)
       cGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTg                                                                                                                                     <  2:1129074/151‑1 (MQ=255)
        gCTACCAGTGGCGAGCTGACATATGAAGCCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGCGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATACCTGGATCGGCATGTGGCAGTTGTTGACCGGCTACGTTAAACAGCTg                                                                                                                                     >  1:698556/1‑150 (MQ=255)
          tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGc                                                                                                                                   >  1:97443/1‑150 (MQ=255)
           aCCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGc                                                                                                                                   <  2:54918/149‑1 (MQ=255)
           aCCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCt                                                                                                                                  <  1:1046005/150‑1 (MQ=255)
            ccAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCttt                                                                                                                                >  2:873395/1‑151 (MQ=255)
             cAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTg                                                                                                                               >  1:1177959/1‑151 (MQ=255)
              aGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTgc                                                                                                                              <  2:349511/151‑1 (MQ=255)
               gTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTgcg                                                                                                                             <  2:249860/151‑1 (MQ=255)
                  gCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCt                                                                                                                          <  1:142284/151‑1 (MQ=255)
                   cGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCt                                                                                                                          >  1:740/1‑150 (MQ=255)
                   cGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTg                                                                                                                         <  2:338301/151‑1 (MQ=255)
                     aGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGAt                                                                                                                       <  2:160266/151‑1 (MQ=255)
                      gCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGAtg                                                                                                                      >  2:464023/1‑151 (MQ=255)
                      gCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGAtg                                                                                                                      <  1:619520/151‑1 (MQ=255)
                      gCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGAtg                                                                                                                      >  2:773452/1‑151 (MQ=255)
                              atGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                                                                               <  2:1163077/150‑1 (MQ=255)
                                 aaGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGt                                                                                                           <  2:245113/151‑1 (MQ=255)
                                      tGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTg                                                                                                      >  2:280410/1‑151 (MQ=255)
                                       ggATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGAtt                                                                                                       <  1:550967/149‑1 (MQ=255)
                                           cGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                                                                 <  2:508131/151‑1 (MQ=255)
                                               ttcttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCa                                                                                              <  1:380990/150‑1 (MQ=255)
                                                      ccTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTggtggt                                                                                      >  2:470976/1‑151 (MQ=255)
                                                      ccTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTggtggt                                                                                      <  1:656670/151‑1 (MQ=255)
                                                       ctctGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGtt                                                                                     >  2:517128/1‑151 (MQ=255)
                                                         ctGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGttt                                                                                    >  2:995447/1‑150 (MQ=255)
                                                         ctGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGttt                                                                                    >  1:991603/1‑150 (MQ=255)
                                                           gCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTa                                                                                   <  2:225617/149‑1 (MQ=255)
                                                           gCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACg                                                                                 >  1:1209049/1‑151 (MQ=255)
                                                                cACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                                                                            <  2:1266959/151‑1 (MQ=255)
                                                                     aaGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATgg                                                                       >  2:511649/1‑151 (MQ=255)
                                                                      aGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGTTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGa                                                                      <  1:998326/151‑1 (MQ=255)
                                                                        tgtTCACCCTGCTGGCGCTGTGTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGTTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTTGTTGCACTGGTGGTTTACGTGATTTATGGAtt                                                                    >  1:73510/1‑151 (MQ=255)
                                                                            cACCCTGCTGGAGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTAGtt                                                                <  2:517358/151‑1 (MQ=255)
                                                                               ccTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg                                                             <  2:845881/151‑1 (MQ=255)
                                                                                ctgctgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtgg                                                            >  1:612949/1‑151 (MQ=255)
                                                                                  gctgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtg                                                          >  2:384083/1‑151 (MQ=255)
                                                                                   ctgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgt                                                         >  2:578127/1‑151 (MQ=255)
                                                                                    tgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtg                                                        >  1:423638/1‑151 (MQ=255)
                                                                                    tgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgcggtgtg                                                        >  2:162410/1‑151 (MQ=255)
                                                                                     ggCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtgg                                                       <  2:756390/151‑1 (MQ=255)
                                                                                     ggCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtgg                                                       <  1:785040/151‑1 (MQ=255)
                                                                                          tGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgt                                                  >  1:599714/1‑151 (MQ=255)
                                                                                          tGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtg                                                   >  1:1217555/1‑150 (MQ=255)
                                                                                           gTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgtg                                                 >  2:1143446/1‑151 (MQ=255)
                                                                                           gTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgt                                                  <  2:279699/150‑1 (MQ=255)
                                                                                            ttttCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgtg                                                 >  1:724870/1‑150 (MQ=255)
                                                                                             tttCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgtgt                                                <  2:130806/150‑1 (MQ=255)
                                                                                                cTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatg                                            >  2:1156525/1‑151 (MQ=255)
                                                                                                  aTCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatga                                           <  1:661925/150‑1 (MQ=255)
                                                                                                  aTCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatga                                           <  2:155022/150‑1 (MQ=255)
                                                                                                  aTCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgatg                                            <  1:730466/149‑1 (MQ=255)
                                                                                                  aTCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgat                                             <  2:698592/148‑1 (MQ=255)
                                                                                                  aTCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgat                                             <  2:974267/148‑1 (MQ=255)
                                                                                                       gATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGc                                     >  1:93665/1‑151 (MQ=255)
                                                                                                        aTCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGcc                                    >  1:378179/1‑151 (MQ=255)
                                                                                                         tCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGcc                                    >  2:866287/1‑150 (MQ=255)
                                                                                                            aTGCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTc                                >  2:142820/1‑151 (MQ=255)
                                                                                                              gCCTGGATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCa                               <  1:49018/150‑1 (MQ=255)
                                                                                                               ccTGGATCTGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCa                               <  1:530584/149‑1 (MQ=255)
                                                                                                               ccTGGAGCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTc                                >  1:178851/1‑148 (MQ=255)
                                                                                                                  ggATCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGaa                          <  1:745566/151‑1 (MQ=255)
                                                                                                                     tCGGCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAAttt                       >  2:313390/1‑151 (MQ=255)
                                                                                                                       ggCATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGa                     <  2:613336/151‑1 (MQ=255)
                                                                                                                         cATGTGGCAGGTGTTGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATg                   <  2:984671/151‑1 (MQ=255)
                                                                                                                                       tGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGt     >  2:630578/1‑151 (MQ=255)
                                                                                                                                       tGACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGt     >  1:263784/1‑151 (MQ=255)
                                                                                                                                        gACCGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTg    >  2:1178723/1‑151 (MQ=255)
                                                                                                                                          ccGGCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGcc  <  2:336371/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCC  >  NC_000913/755663‑755951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: