Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	781,084	T→C	noncoding (17/76 nt)	valZ →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,084	0	T	C	97.6%	272.5 / ‑6.4	83	noncoding (17/76 nt)	valZ	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (46/35);  total (47/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTT  >  NC_000913/780936‑781227                                                                                                                                                     |                                                                                                                                                aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCgg                                                                                                                                               <  2:96317/151‑1 (MQ=255) aGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCgg                                                                                                                                               <  2:1289515/151‑1 (MQ=255)  gATGTTAAGCGCGTAGGATAACGTGGCGTGAGCAACGGCCCATGGGGCGAGGGAAGAGAGTCGTCCTGCACGCCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                              <  1:27443/151‑1 (MQ=38)   aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                              >  2:1051168/1‑150 (MQ=255)   aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                              >  2:305688/1‑150 (MQ=255)     gTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGgaga                                                                                                                                           <  2:93794/151‑1 (MQ=255)     gTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGgag                                                                                                                                            <  2:1052977/150‑1 (MQ=255)      ttAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGgag                                                                                                                                            >  2:1053512/1‑149 (MQ=255)        aaGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGc                                                                                                                                         <  2:1115018/150‑1 (MQ=255)            gtgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTc                                                                                                                                    >  2:219175/1‑151 (MQ=255)            gtgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTc                                                                                                                                    >  2:995783/1‑151 (MQ=255)                 ggATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCtt                                                                                                                                >  2:702416/1‑150 (MQ=255)                 ggATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCtt                                                                                                                                >  2:1170440/1‑150 (MQ=255)                  gATAACGTTGCGTCAATAACGTCGCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTAc                                                                                                                              <  1:740931/151‑1 (MQ=255)                   aTAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTAc                                                                                                                              >  2:620877/1‑150 (MQ=255)                     aaCGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGGGATTAGCTCAGCCGGGAGAGCACCTCCCTTACa                                                                                                                             >  1:194839/1‑149 (MQ=255)                       cGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCTCCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAagga                                                                                                                         <  2:830190/151‑1 (MQ=255)                         ttGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAaggagg                                                                                                                       >  1:1153388/1‑151 (MQ=255)                         ttGCCTCAGCAACGGCCCGTAGGGCGACCGAACCGAGTTATCCTGCACGACCCACCACTTATGACGGCGGGTTCGGTGAAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAag                                                                                                                           <  1:414844/147‑1 (MQ=37)                            cGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAggggg                                                                                                                    >  1:366989/1‑151 (MQ=255)                                aGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                                                                                                >  2:116284/1‑151 (MQ=255)                                aGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                                                                                                >  2:172161/1‑151 (MQ=255)                                   aaCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                                                                                             >  2:285596/1‑151 (MQ=255)                                    aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                                                                                             >  2:986113/1‑150 (MQ=255)                                     cGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                                                                                           <  2:1035674/151‑1 (MQ=255)                                      gtagcGTAGGGGGAGCGATGAGAGTCATGCTGCACGACCCGCCACAAATGACGGTGGGTTCGGAGAAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcg                                                                                                              <  1:1123422/143‑1 (MQ=21)                                      ggCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                                                                                           >  1:885794/1‑150 (MQ=255)                                        cccGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTGCg                                                                                                         >  1:1219776/1‑150 (MQ=255)                                           gTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                                                                                     >  1:600545/1‑151 (MQ=255)                                            tAGGGCGAGCGAAGCGAGTCATCCTGCACGACGCACCACTATTGACGGTGGGTTCCGTGGAAGTATCTTTTAGTATACAGCGCAGTATCGCGAGATCAGCACAGCTGGGTGAGCACCTCCCTTACAAGGAGGGGGACGGCGGTTCTATcc                                                                                                     >  1:77910/1‑150 (MQ=21)                                             aGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                                                                                                    <  2:1098078/150‑1 (MQ=255)                                              gggCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                                                                                                    <  2:760836/149‑1 (MQ=255)                                                 cgagggaAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtca                                                                                                <  2:1259590/150‑1 (MQ=255)                                                     cgaagcgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc                                                                                           <  2:962074/151‑1 (MQ=255)                                                       aagcgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca                                                                                         <  1:583487/151‑1 (MQ=255)                                                          cgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccacc                                                                                       >  2:311909/1‑150 (MQ=255)                                                          cgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACccacca                                                                                      <  2:872641/151‑1 (MQ=255)                                                            aGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACccacca                                                                                      >  2:1164581/1‑149 (MQ=255)                                                             gTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACt                                                                                    <  1:1053470/150‑1 (MQ=255)                                                                  ccTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTc                                                                              <  2:1159903/151‑1 (MQ=255)                                                                    tGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGt                                                                            >  2:1100974/1‑151 (MQ=255)                                                                          aCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                      <  1:286433/151‑1 (MQ=255)                                                                          aCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                      >  2:720661/1‑151 (MQ=255)                                                                           cccACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                      <  1:1267786/150‑1 (MQ=255)                                                                            ccaccaCTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCt                                                                       >  1:239638/1‑148 (MQ=255)                                                                            ccaccaCTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                      >  2:351415/1‑149 (MQ=255)                                                                                    aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTag                                                             <  2:952209/150‑1 (MQ=255)                                                                                       gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGCTCCCGTCATCACCCACCACTCGGGTCGGTAGCTCAGGTGGTagag                                                           >  2:682780/1‑149 (MQ=255)                                                                                           gTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                     <  1:723166/151‑1 (MQ=255)                                                                                           gTGGGTACGGTGGATGTTCTTGGTTGTCTCCAGCGCAGTAGCGTGCTCTTCGCATAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCa                                                         <  2:64993/147‑1 (MQ=14)                                                                                            tGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                     >  2:1270462/1‑150 (MQ=255)                                                                                              ggTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                   >  2:1218527/1‑150 (MQ=255)                                                                                                 tCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                <  2:922576/150‑1 (MQ=255)                                                                                                   ggTGGAAGTAGTTTGTAGTATCCAGCGAAGTACCGGGTAATTAGCTCAGCCTGGAGAGCACCCCCCTTACAAGGATGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                             <  1:1112258/151‑1 (MQ=14)                                                                                                     tGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGGTGACTTTTaa                                             >  2:1062382/1‑149 (MQ=255)                                                                                                     tGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGGGCAGTTGACTTTTTAt                                            >  1:935701/1‑150 (MQ=255)                                                                                                      ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCa                                          <  1:85035/151‑1 (MQ=255)                                                                                                       gAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCa                                          >  2:707526/1‑150 (MQ=255)                                                                                                        aaGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGGCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                        >  2:361784/1‑151 (MQ=255)                                                                                                            agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                     >  1:616086/1‑150 (MQ=38)                                                                                                            agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                     >  1:672328/1‑150 (MQ=38)                                                                                                             gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                    <  2:1012244/150‑1 (MQ=38)                                                                                                                tGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                 >  2:909901/1‑150 (MQ=25)                                                                                                                   agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCCCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCCTCCCCCACCCCGCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                              <  1:579842/150‑1 (MQ=11)                                                                                                                        ccAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGAGCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                          <  2:1244525/149‑1 (MQ=25)                                                                                                                         cAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGCAACCATCATCACCCACCACTCGGGGCGGTAGTTCAGTTTGTAGAGAAGTTGCAGTTTAATCAATTGGTCGCAgggt                            >  1:775470/1‑144 (MQ=11)                                                                                                                           gcgcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                     >  2:311681/1‑151 (MQ=25)                                                                                                                           gcgcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                     <  1:164720/151‑1 (MQ=25)                                                                                                                           gcgcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                     <  2:1153422/151‑1 (MQ=25)                                                                                                                            cgcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                    >  2:548769/1‑151 (MQ=25)                                                                                                                             gcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                    <  2:724870/150‑1 (MQ=25)                                                                                                                             gcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                    >  1:23573/1‑150 (MQ=25)                                                                                                                             gcAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                   >  1:186673/1‑151 (MQ=25)                                                                                                                               aGTATCGAGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCCCCCACCACACGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                 <  2:351276/151‑1 (MQ=9)                                                                                                                                gTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc                >  2:618426/1‑151 (MQ=18)                                                                                                                                gTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc                <  2:885834/151‑1 (MQ=18)                                                                                                                                     gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGGTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGGATCCTGCACGAccc           >  1:72522/1‑151 (MQ=255)                                                                                                                                      ggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTTATCAATTGGTCGCAGGTTCGAATCCTGCACGAacc           >  2:1046949/1‑150 (MQ=16)                                                                                                                                        tGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccacc        >  1:192402/1‑151 (MQ=18)                                                                                                                                         gATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccacca       <  2:525813/151‑1 (MQ=18)                                                                                                                                          aTTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccacc        >  2:669254/1‑149 (MQ=18)                                                                                                                                           ttAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGGTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAGGActccccgg      >  1:15898/1‑143 (MQ=11)                                                                                                                                               ttCAGCCGGGAGAGCACCTCCCTTACAAGGGGGGGGTCGGCGGGTCCATCCCGTCATTACCCACCCCTCCGGTCGATAGCATCCCTGGTAGAGCAGGTGGCTTTTCCTCCAACTGTCGCCGGATGGGATCCCTCACGCCGCACCATtttt  >  1:1270133/2‑150 (MQ=2)                                                                                                                                                     |                                                                                                                                                AGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTT  >  NC_000913/780936‑781227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑