Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,000,789	A→G	Y525C (TAC→TGC)	elfC →	putative outer membrane fimbrial subunit export usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,000,789	0	A	G	100.0%	280.8 / NA	76	Y525C (TAC→TGC)	elfC	putative outer membrane fimbrial subunit export usher protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/42);  total (34/42)

CCGACACCATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTACGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCAACCTGA  >  NC_000913/1000645‑1000936                                                                                                                                                 |                                                                                                                                                    ccGACACCATGTATAAACATATGGATGGCTACGACTTTAATGACGGTGATGATGAAGATACGCCGATGTGGTAGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATCTCTCCCAGCAATTAGGCGAGTGCGGTTc                                                                                                                                               <  2:258946/151‑1 (MQ=255)    acacCATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGtt                                                                                                                                            >  1:260923/1‑151 (MQ=255)    acacCATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGtt                                                                                                                                            >  2:167621/1‑151 (MQ=255)        cATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTtttat                                                                                                                                        >  2:200198/1‑151 (MQ=255)        cATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTtttat                                                                                                                                        >  1:1275540/1‑151 (MQ=255)         aTGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTtttatt                                                                                                                                       <  1:664944/151‑1 (MQ=255)            tataAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTtttattta                                                                                                                                     >  2:399044/1‑150 (MQ=255)              taAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAg                                                                                                                                   >  1:1245747/1‑150 (MQ=255)                  cATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTa                                                                                                                              <  1:310326/151‑1 (MQ=255)                   atatGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGt                                                                                                                               >  2:534642/1‑149 (MQ=255)                     atggatggCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGcc                                                                                                                           <  1:1102714/151‑1 (MQ=255)                     atggatggCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGcc                                                                                                                           >  1:993468/1‑151 (MQ=255)                       ggatggCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCa                                                                                                                          <  1:637930/150‑1 (MQ=255)                           ggCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCaa                                                                                                                      >  1:1230771/1‑150 (MQ=255)                              tACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACtt                                                                                                                  >  2:1250844/1‑151 (MQ=255)                              tACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACtt                                                                                                                  >  2:1083301/1‑151 (MQ=255)                                 gAATTTAATGACGGTGATGATGAAGATAAGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACt                                                                                                               >  1:1180861/1‑151 (MQ=255)                                       aaTGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCAt                                                                                                          >  1:522439/1‑150 (MQ=255)                                               tgatgatgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCa                                                                                                  >  2:237167/1‑150 (MQ=255)                                                 atgatgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAAc                                                                                                >  2:12753/1‑150 (MQ=255)                                                   gatgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAgg                                                                                             >  1:531552/1‑151 (MQ=255)                                                   gatgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAg                                                                                              <  1:228872/150‑1 (MQ=255)                                                    atgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAgg                                                                                             >  1:73682/1‑150 (MQ=255)                                                    atgaAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGa                                                                                            <  2:191745/151‑1 (MQ=255)                                                       aaGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCg                                                                                         <  1:1074628/151‑1 (MQ=255)                                                         gATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGc                                                                                       <  2:227503/151‑1 (MQ=255)                                                            aCGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTa                                                                                     <  1:832786/150‑1 (MQ=255)                                                            aCGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTAt                                                                                    <  2:254669/151‑1 (MQ=255)                                                              gCCGGTGAGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCTGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTAtt                                                                                   >  1:680623/1‑150 (MQ=255)                                                                cGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACa                                                                                <  1:312171/151‑1 (MQ=255)                                                                   tgtgGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGtt                                                                             >  1:264924/1‑151 (MQ=255)                                                                      ggTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTg                                                                           <  2:1185702/150‑1 (MQ=255)                                                                      ggTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTg                                                                           <  1:818562/150‑1 (MQ=255)                                                                       gTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGc                                                                         <  2:95555/151‑1 (MQ=255)                                                                         cgcgTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCt                                                                        <  2:999522/150‑1 (MQ=255)                                                                            gTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTacaa                                                                    <  2:509029/151‑1 (MQ=255)                                                                                  aCAATTTGTTTTATACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCTGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTGCAACACGc                                                               <  1:543497/150‑1 (MQ=255)                                                                                    aaTTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCaa                                                             >  1:528633/1‑150 (MQ=255)                                                                                     aTTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAAt                                                           >  2:1267114/1‑151 (MQ=255)                                                                                       ttGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAAtt                                                          <  1:534614/150‑1 (MQ=255)                                                                                       ttGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAAtt                                                          <  2:234126/150‑1 (MQ=255)                                                                                       ttGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAAtt                                                          <  1:1245302/150‑1 (MQ=255)                                                                                       ttGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAAtt                                                          <  1:1200120/150‑1 (MQ=255)                                                                                        tGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTa                                                         >  2:1053575/1‑150 (MQ=255)                                                                                         gTTTTACACCAAACGCGGCAAACTGCAGGACAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAACCTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTaa                                                        >  1:975040/1‑150 (MQ=255)                                                                                            ttACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGa                                                     <  1:101406/150‑1 (MQ=255)                                                                                             tACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGAt                                                    <  2:884211/150‑1 (MQ=255)                                                                                              acacCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCt                                                  <  1:100429/151‑1 (MQ=255)                                                                                              acacCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCt                                                  >  2:247630/1‑151 (MQ=255)                                                                                               cacCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTGGCCAGCAAACTTACTGGCATACGGATCAACAGGAGCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATc                                                   <  1:843178/149‑1 (MQ=255)                                                                                               cacCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCtt                                                 <  1:441823/151‑1 (MQ=255)                                                                                                         ggCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTgggg                                        <  2:998572/150‑1 (MQ=255)                                                                                                            aaaCTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGAttt                                    >  2:601302/1‑151 (MQ=255)                                                                                                             aaCTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTc                                   >  2:135796/1‑151 (MQ=255)                                                                                                             aaCTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTc                                   <  1:1203903/151‑1 (MQ=255)                                                                                                                 gCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCt                                 <  1:1131925/149‑1 (MQ=255)                                                                                                                 gCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCt                                 <  1:127572/149‑1 (MQ=255)                                                                                                                 gCAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTg                                <  1:335166/150‑1 (MQ=255)                                                                                                                  cAGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGa                              <  2:318868/151‑1 (MQ=255)                                                                                                                   aGGTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGa                              >  2:285103/1‑150 (MQ=255)                                                                                                                     gTCAATATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACt                           <  2:73680/151‑1 (MQ=255)                                                                                                                        aaTATCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTAc                         <  1:872275/150‑1 (MQ=255)                                                                                                                          tatCTCCCAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGt                      >  2:608733/1‑151 (MQ=255)                                                                                                                               cccAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTc                 >  1:941822/1‑151 (MQ=255)                                                                                                                               cccAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTc                 >  2:585870/1‑151 (MQ=255)                                                                                                                                 cAGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTccc               <  2:42042/151‑1 (MQ=255)                                                                                                                                  aGCAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCg              >  1:21428/1‑151 (MQ=255)                                                                                                                                    cAATTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTg            <  1:591168/151‑1 (MQ=255)                                                                                                                                     aaTTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTgg           <  1:1249382/151‑1 (MQ=255)                                                                                                                                     aaTTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTgg           >  1:149946/1‑151 (MQ=255)                                                                                                                                      aTTAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGt          >  2:841556/1‑151 (MQ=255)                                                                                                                                        tAGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTc         >  1:980038/1‑150 (MQ=255)                                                                                                                                         aGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCaa       <  1:12758/151‑1 (MQ=255)                                                                                                                                         aGGCGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCa        <  1:885249/150‑1 (MQ=255)                                                                                                                                            cGAGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCAACCt    <  2:778799/151‑1 (MQ=255)                                                                                                                                              aGTGCGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCAACCTGa  <  2:125340/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCGACACCATGTATAAACATATGGATGGCTACGAATTTAATGACGGTGATGATGAAGATACGCCGATGTGGTCGCGTTATTACAATTTGTTTTACACCAAACGTGGCAAACTGCAGGTCAATATCTCCCAGCAATTAGGCGAGTACGGTTCGTTTTATTTAAGTGGTAGCCAGCAAACTTACTGGCATACGGATCAACAGGATCGGCTATTACAGTTTGGCTACAACACGCAAATTAAAGATCTTTCGCTGGGGATTTCCTGGAACTACAGTAAGTCCCGTGGTCAACCTGA  >  NC_000913/1000645‑1000936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑