Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,013,234	T→C	S412P (TCA→CCA)	pqiA →	paraquat‑inducible, SoxRS‑regulated inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,013,234	0	T	C	100.0%	361.4 / NA	93	S412P (TCA→CCA)	pqiA	paraquat‑inducible, SoxRS‑regulated inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/40);  total (53/40)

CGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCA  >  NC_000913/1013088‑1013380                                                                                                                                                   |                                                                                                                                                   cgGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAAGGTCAATGTTTTCTGCTATGACGTTTGATCCGCGGTTGTCGTGGGTTCGTCAACCGGAACCa                                                                                                                                                  <  1:231250/149‑1 (MQ=255)  ggTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCagag                                                                                                                                               <  2:1268998/151‑1 (MQ=255)        ctcGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATg                                                                                                                                           >  1:449541/1‑149 (MQ=255)         tcGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATgagg                                                                                                                                        >  1:223506/1‑151 (MQ=255)          cGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATgagg                                                                                                                                        >  2:1031590/1‑150 (MQ=255)          cGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATgagg                                                                                                                                        >  2:142437/1‑150 (MQ=255)            gcgcTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGt                                                                                                                                     >  2:1019770/1‑151 (MQ=255)             cgcTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTc                                                                                                                                    <  1:78137/151‑1 (MQ=255)               cTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCt                                                                                                                                  >  2:745208/1‑151 (MQ=255)                     cGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACa                                                                                                                              <  1:244661/149‑1 (MQ=255)                      gTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTa                                                                                                                           <  2:528603/151‑1 (MQ=255)                          gggAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATgg                                                                                                                        >  2:714618/1‑150 (MQ=255)                           ggAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGaa                                                                                                                      <  1:1148075/151‑1 (MQ=255)                            gAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAAt                                                                                                                     <  1:213025/151‑1 (MQ=255)                            gAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAAt                                                                                                                     >  2:431888/1‑151 (MQ=255)                            gAGGCTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAAt                                                                                                                     >  1:255253/1‑151 (MQ=255)                                 ttAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCtaat                                                                                                                >  2:962398/1‑151 (MQ=255)                                      gAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATgg                                                                                                           <  1:1125031/151‑1 (MQ=255)                                       aGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATggg                                                                                                          <  1:477820/151‑1 (MQ=255)                                        gTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATgggg                                                                                                         >  2:650287/1‑151 (MQ=255)                                        gTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATgggg                                                                                                         >  1:50073/1‑151 (MQ=255)                                        gTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATgggg                                                                                                         >  2:713358/1‑151 (MQ=255)                                          aTTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGaa                                                                                                       <  2:804204/151‑1 (MQ=255)                                          aTTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGaa                                                                                                       <  2:1004698/151‑1 (MQ=255)                                          aTTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGa                                                                                                        >  1:63505/1‑150 (MQ=255)                                          aTTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGa                                                                                                        <  2:1071598/150‑1 (MQ=255)                                          aTTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGa                                                                                                        <  1:1085776/150‑1 (MQ=255)                                             tATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGcc                                                                                                    >  2:975239/1‑151 (MQ=255)                                              attcGGCAATGGGAGCATTAATGTTTGCTTTAGTCGGCATAATGACATTGTTTTCTGCTATGACGATTGACCCGCGTTTGTCGTGGGGTTGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAgac                                                                                                    >  1:645803/4‑148 (MQ=255)                                                ccGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCaa                                                                                                  <  1:714585/150‑1 (MQ=255)                                                   gCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATc                                                                                              >  1:853008/1‑151 (MQ=255)                                                    cAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATcc                                                                                             >  1:321730/1‑151 (MQ=255)                                                    cAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATcc                                                                                             <  1:737160/151‑1 (MQ=255)                                                      aTGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAg                                                                                           <  2:540116/151‑1 (MQ=255)                                                      aTGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGCTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATc                                                                                              >  1:310797/1‑148 (MQ=255)                                                            gCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGt                                                                                      <  1:729597/150‑1 (MQ=255)                                                             cATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTg                                                                                     >  2:156002/1‑150 (MQ=255)                                                              aTTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATACAGAAAgaga                                                                                    >  1:8890/1‑150 (MQ=255)                                                                tAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTga                                                                                    >  1:1050936/1‑148 (MQ=255)                                                                 aaTGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTgaagaa                                                                                <  1:720786/151‑1 (MQ=255)                                                                 aaTGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTgaaga                                                                                 >  1:790763/1‑150 (MQ=255)                                                                  aTGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTgaaga                                                                                 <  2:391675/149‑1 (MQ=255)                                                                     tttGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTgg                                                                            >  1:383230/1‑151 (MQ=255)                                                                      ttGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTgg                                                                            <  1:638845/150‑1 (MQ=255)                                                                      ttGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAAGCCAGAAAGTGAAGAACTgg                                                                            <  1:311248/150‑1 (MQ=255)                                                                        gCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGt                                                                           >  2:616386/1‑149 (MQ=255)                                                                          tttAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTcc                                                                       >  1:347749/1‑151 (MQ=255)                                                                           ttAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTccc                                                                      >  2:1126545/1‑151 (MQ=255)                                                                            tAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCg                                                                     >  2:185401/1‑151 (MQ=255)                                                                             aGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCgt                                                                    <  1:743211/151‑1 (MQ=255)                                                                             aGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCg                                                                     <  2:666147/150‑1 (MQ=255)                                                                              gtcgtcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCgtg                                                                   <  2:5433/151‑1 (MQ=255)                                                                              gtcgtcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCgtg                                                                   <  2:293301/151‑1 (MQ=255)                                                                              gtcgtcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGAGAAGAACTGGTCTCCCgtg                                                                   >  2:339479/1‑151 (MQ=255)                                                                                cgtcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGtgtg                                                                 <  1:1194629/151‑1 (MQ=255)                                                                                cgtcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGtgtg                                                                 >  1:354029/1‑151 (MQ=255)                                                                                  tcATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTgg                                                                >  1:1216648/1‑150 (MQ=255)                                                                                        tGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTc                                                         >  2:913200/1‑151 (MQ=255)                                                                                          aCAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCt                                                       <  1:1024694/151‑1 (MQ=255)                                                                                           cAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCt                                                       >  2:527150/1‑150 (MQ=255)                                                                                            aaTGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTAt                                                     <  2:1282416/151‑1 (MQ=255)                                                                                              tGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCg                                                   >  1:136550/1‑151 (MQ=255)                                                                                               gTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGt                                                  >  2:1125015/1‑151 (MQ=255)                                                                                                  ttCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCAc                                               >  1:1185200/1‑151 (MQ=255)                                                                                                  ttCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCAc                                               >  1:927547/1‑151 (MQ=255)                                                                                                    cTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACgg                                             >  2:304073/1‑151 (MQ=255)                                                                                                    cTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACgg                                             >  2:43269/1‑151 (MQ=255)                                                                                                      gCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGgc                                            <  2:853046/150‑1 (MQ=255)                                                                                                        tATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCt                                         >  1:475142/1‑151 (MQ=255)                                                                                                           gACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCAt                                      >  1:923298/1‑151 (MQ=255)                                                                                                             cGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTg                                    <  2:1266862/151‑1 (MQ=255)                                                                                                             cGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTg                                    >  1:272318/1‑151 (MQ=255)                                                                                                               tttGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTggg                                  >  2:943517/1‑151 (MQ=255)                                                                                                                ttGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTgggg                                 >  1:744592/1‑151 (MQ=255)                                                                                                                ttGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTgggg                                 >  2:793271/1‑151 (MQ=255)                                                                                                                 tGACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTgggg                                 <  1:447420/150‑1 (MQ=255)                                                                                                                  gACCCGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGcc                               >  2:209304/1‑151 (MQ=255)                                                                                                                     ccGCGTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTgg                            <  1:141015/151‑1 (MQ=255)                                                                                                                       gcgTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGt                          <  2:522149/151‑1 (MQ=255)                                                                                                                         gTTTGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCGATCGTCACGGCGCTCATTGGGGCCTGGGTTc                        <  2:940050/151‑1 (MQ=255)                                                                                                                            tGTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCtt                      <  1:1261854/150‑1 (MQ=255)                                                                                                                             gTCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttt                     <  2:1059352/150‑1 (MQ=255)                                                                                                                              tCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttttt                   >  1:587119/1‑151 (MQ=255)                                                                                                                              tCGTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttttt                   >  2:886456/1‑151 (MQ=255)                                                                                                                                gTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCtttttt                  >  2:198881/1‑150 (MQ=255)                                                                                                                                gTGGGATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTa                 <  1:1282169/151‑1 (MQ=255)                                                                                                                                   ggATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCa              <  1:309560/151‑1 (MQ=255)                                                                                                                                   ggATCGTCAACCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCa              <  1:858174/151‑1 (MQ=255)                                                                                                                                           aaCCTGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGcc      >  1:868884/1‑151 (MQ=255)                                                                                                                                               tGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATc   >  2:296021/1‑150 (MQ=255)                                                                                                                                               tGAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATc   >  2:428430/1‑150 (MQ=255)                                                                                                                                                gAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATc   >  2:1174859/1‑149 (MQ=255)                                                                                                                                                gAACCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCa  <  1:975202/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCA  >  NC_000913/1013088‑1013380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑