Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,782	A→G	E224G (GAA→GGA)	yceG →	septation protein, ampicillin sensitivity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,782	0	A	G	100.0%	279.4 / NA	75	E224G (GAA→GGA)	yceG	septation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/33);  total (42/33)

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAAC  >  NC_000913/1154635‑1154927                                                                                                                                                    |                                                                                                                                                  tCGCGTTACTCAAGCGGGCGCAAAAAAAAAGGGTAAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCTTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAc                                                                                                                                                 <  2:1289486/150‑1 (MQ=255)  cgcgTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGAcgc                                                                                                                                               >  2:207692/1‑151 (MQ=255)            aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTg                                                                                                                                     >  2:342931/1‑151 (MQ=255)            aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTg                                                                                                                                     >  2:364584/1‑151 (MQ=255)                gagcgCAAAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCt                                                                                                                                  <  2:624005/150‑1 (MQ=255)                    gcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGt                                                                                                                              >  2:921451/1‑150 (MQ=255)                      acaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                           >  1:1212941/1‑151 (MQ=255)                        aaaaaaaTGGTGAAGGCGTTCGATAGCGCCTGGGAAGGGGGTGCGGACGGTTTGCTTTATAAAAATAAAAACCATTGGGTGACGAGGGCTTCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                            <  1:720882/145‑1 (MQ=255)                        aaGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAttt                                                                                                                          <  1:872693/150‑1 (MQ=255)                         aGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTAt                                                                                                                        >  2:878332/1‑151 (MQ=255)                           aaaaGGGTGAAAGCGGTCGATAGCGCCTGGGAGGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTGGGTGACGATGGCATCAATTACCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCa                                                                                                                      <  1:530625/151‑1 (MQ=255)                            aaaTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCa                                                                                                                      >  2:468521/1‑150 (MQ=255)                                   gAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGttt                                                                                                               >  2:969132/1‑150 (MQ=255)                                    aaaGCTGTCTATAGCGCAGGGGAGGGGCGGGGGGTCGGTATGCGTTAAAAATATAAAATCGGATTGGATACGCGGGCATCACTTACGGAAAAAAAAGCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTa                                                                                                              <  1:901123/150‑1 (MQ=255)                                    aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTAc                                                                                                             >  1:87070/1‑151 (MQ=255)                                           tCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTgg                                                                                                      >  2:622246/1‑151 (MQ=255)                                            cGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGATGCCTCAGTATTTATCAACCGTTTACGCACTGGt                                                                                                     >  1:1167451/1‑151 (MQ=255)                                                 gcgcCTGGGAAGGGCGTGCGGACGGTCTGCCTTAAAAAGATAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTAt                                                                                                   <  2:513109/148‑1 (MQ=255)                                                  cgcCGGGGAGGGGCGTGCGGACGGTCTGCCTTAAAAAGATAAAACCCAGTTGGTGAGGATGGCATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTa                                                                                                    <  1:95201/146‑1 (MQ=255)                                                   gcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGcc                                                                                              >  2:390123/1‑151 (MQ=255)                                                      tGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGc                                                                                           >  2:548148/1‑151 (MQ=255)                                                      tGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGc                                                                                           >  2:910477/1‑151 (MQ=255)                                                          aaGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgac                                                                                       <  2:188045/151‑1 (MQ=255)                                                            gggCGTGCGGACGGTTTGTTTTATAAAGAAAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                     <  2:242578/151‑1 (MQ=255)                                                                gTGGGGAGGGTCTGCCTTAAAAAGAAAAAAACCAGTTGGTGACGTTGGCATCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgac                                                                                   <  2:871511/149‑1 (MQ=255)                                                                   cGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccgac                                                                              >  1:65542/1‑151 (MQ=255)                                                                        ggTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTg                                                                          >  2:918526/1‑150 (MQ=255)                                                                        ggTCTGCCTAAAAAAGAAAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGt                                                                           <  1:1228753/149‑1 (MQ=255)                                                                           cggCCTTATAAAGAAAAAAACCAGGTGTTGACGATGTCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGt                                                                           <  1:696502/144‑1 (MQ=255)                                                                                  ataAAGATAAAAACCAGTTGGTGACGATGGCACCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                                  <  2:1179262/148‑1 (MQ=255)                                                                                  aaaaaGAAAAAAACCAGTTGGTGACGATGGCATAAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGa                                                                <  1:821247/148‑1 (MQ=255)                                                                                   taAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                              >  2:278978/1‑151 (MQ=255)                                                                                    aaaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATgg                                                             >  2:1219924/1‑151 (MQ=255)                                                                                    aaaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATgg                                                             <  2:899505/151‑1 (MQ=255)                                                                                     aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                            >  2:783560/1‑151 (MQ=255)                                                                                     aaGATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                            <  2:169501/151‑1 (MQ=255)                                                                                      aGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                            >  2:397309/1‑150 (MQ=255)                                                                                          aaaacCCGGTTGGTGACGATGGCATAAATTATGAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGagag                                                        <  1:522317/150‑1 (MQ=255)                                                                                          aaaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                       >  1:248031/1‑151 (MQ=255)                                                                                          aaaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                       >  1:938782/1‑151 (MQ=255)                                                                                          aaaaaCCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGagag                                                        <  1:1118629/150‑1 (MQ=255)                                                                                           aaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCg                                                      >  2:515816/1‑151 (MQ=255)                                                                                               ccAGTTGGGGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTta                                                   <  2:1225324/150‑1 (MQ=255)                                                                                                cAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTtat                                                  >  1:744076/1‑150 (MQ=255)                                                                                                   ttGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATg                                              >  1:58417/1‑151 (MQ=255)                                                                                                    tGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATgg                                             >  1:1051763/1‑151 (MQ=255)                                                                                                       ggACGATGACATCATTTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                          <  1:384186/150‑1 (MQ=255)                                                                                                        gACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                          >  2:1266214/1‑150 (MQ=255)                                                                                                        gACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                          >  2:283789/1‑150 (MQ=255)                                                                                                        gACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                          >  1:428916/1‑150 (MQ=255)                                                                                                              ggCATCAATTTTCGAAAAAGAACCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaaa                                         <  1:665222/145‑1 (MQ=255)                                                                                                               gCATGAATGATCGAAAAAGAATCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACtt                                      <  1:8492/147‑1 (MQ=255)                                                                                                               gCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                     <  1:214918/148‑1 (MQ=255)                                                                                                                   cAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                              >  1:662082/1‑151 (MQ=255)                                                                                                                   cAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGACGGGAGAGCGTTATAATGGCAAACTTTCTCGTg                               >  1:6763/1‑150 (MQ=255)                                                                                                                    aaTTATAAAAAAGGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGTAGAGCGTTATAATGGCAAACTTTCTCGTGCa                             <  1:1265179/151‑1 (MQ=255)                                                                                                                      taaTCAAAAAAAAATCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCa                             <  1:730374/147‑1 (MQ=255)                                                                                                                       tATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                          >  1:460872/1‑151 (MQ=255)                                                                                                                       tATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGa                           <  2:1235794/150‑1 (MQ=255)                                                                                                                         tCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGa                           <  2:19725/148‑1 (MQ=255)                                                                                                                           gAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                      >  2:629812/1‑151 (MQ=255)                                                                                                                             aaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGa                     >  1:404191/1‑150 (MQ=255)                                                                                                                              aaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGaaa                   <  2:823833/151‑1 (MQ=255)                                                                                                                               aaaaaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                  <  1:1019458/148‑1 (MQ=255)                                                                                                                               aaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                  >  2:995662/1‑151 (MQ=255)                                                                                                                               aaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAAc                  >  1:872190/1‑151 (MQ=255)                                                                                                                                 gAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGc                >  2:580698/1‑151 (MQ=255)                                                                                                                                  aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc               >  2:1144414/1‑151 (MQ=255)                                                                                                                                     ccgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc            >  1:690963/1‑151 (MQ=255)                                                                                                                                      cgccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACa           <  2:248039/151‑1 (MQ=255)                                                                                                                                         cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg        >  2:589419/1‑151 (MQ=255)                                                                                                                                          gTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt       <  2:860126/151‑1 (MQ=255)                                                                                                                                             gCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGtata    <  2:1224798/151‑1 (MQ=255)                                                                                                                                                aGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGGTGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAac  >  2:632567/1‑150 (MQ=255)                                                                                                                                                aGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAac  >  2:1244073/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAAC  >  NC_000913/1154635‑1154927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑